AT5G05840

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013405
Physical Location & Seq
Reverse (-)
1757065 .. 1760666
3602 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05840.1

Sequence Viewer

Length: 1320 bp
ATGAGGAAACTTTGTCCGAATTACAACCTCGAGGACGGGTTAGAGACCGTCCTAGAGGTTCCCATGCCTGAGGAGTTATTTGCTGCGTCCAAGACAAAACCTGGATGGAACCAAATGAAATCTTACTGGTCAAAACCTACCGCTACTGCTACCGGAACCGCAACAGCCACAAACATGACAAGACTCTTTGGTGGTCGTAATGCAGAGATCCAGCTCCTTCTCGGAGTTGTTGGGGCTCCTTTGATCCCTCTCCCCGTTCAGCCTGACCACCATAACGATTATGAGAATCCCATCCACAAAGACATCAAAGACCAACCTCTCGAGATGTCGATGGCGCAATACATAGTAAAGCAGTACATAGCAGCGGTAGGAGGCGATCGAGCGTTAAACGCGGTAGAGAGCATGTATGCGATGGGAAAAGTGAGGATGACAGCATCAGAGTTTTGCACAGGAGAAGGAAGCTTGAACAGCAAAATGGTAAAGGCAAGGAGTATCAAGAGTGGAGGAGGAGAAGTAGGAGGCTTTGTGCTGTGGCAAAAGGGGATAGAGTTATGGTGTCTTGAGCTTGTCGTTTCAGGCTGCAAAATCAGTGCCGGTAGTGACGCCAAAGTCGCTTGGAGACAAACTCCGTGGCATCCTTCTCATGCCTCTCGTGGTCCTCCTAGACCATTGCGCCGTTTCCTCCAGGGTCTTGATCCAAAATCAACGGCTAATCTATTTGCAAGATCAGTATGCATGGGAGAGAAGAAGATAAATGATGAAGACTGTTTCATACTCAAACTTGATGCTGAGCCATCGGCGCTTAAGGCGAGAAGCAGCAGCAATGTTGAGATCATACGGCACACTGTGTGGGGATGTTTCAGCCAGAGAACAGGTCTTTTGATTCAACTTGAAGACTCTCACCTTCTAAGAATCAAAGCTCAAGACGATAACAGTATATTTTGGGAGACAACAATGGAGTCTTTGATCCAAGATTACAGGACCGTTGATGGTATCTTGGTAGCACACGCTGGTAAAAGCTCGGTGTCTCTGTTTCGGTTTGGTGAGAACTCGGATAATCATTCGAGGACTCGGATGGAAGAGACTTGGGAGATTGAGGAAATGGATTTTAACATTAAGGGTCTGTCTATGGATTGTTTTTTACCGCCTTCTGACCTGAAGAAGGACGATGATGAAGAAGAAGAAATCGAATGTGGTTTAGCTGCTAATAATGAGAAGCTGCCTATGAAAATCCGAAGCGCTTCTTTGAGGATTAGTTCCTCAAAGGTCTTAGCAATTGTGGAAGAAGAAGATGAATCAGAGGTCACTGAAGAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

439

Amino Acids

48.86

Weight (kDa)

5.28

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 135 - 383 2.9e-122 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05840
fragaria_vesca FvH4_7g23730 FvH4_7g23730 FvH4_7g23730
malus_domestica MD01G1144500.v1.1
prunus_persica Prupe.2G243900_v2.0.a1
pyrus_communis pycom01g16410 pycom07g19460
rosa_chinensis RchiOBHm_Chr1g0369031
rosa_laevigata RLG00000027163
rosa_multiflora Rmu_sc0004250.1_g000029
rosa_roxburghii Rroxscaffold_4G00288070
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354100 Rh1BG317500 Rh1CG332000 Rh1DG348400
rosa_wichuraiana Rw1G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 608
AbsI CCTCGAGG 1 cut(s) 29
AccII CGCG 1 cut(s) 392
AciI CCGC 5 cut(s) 141, 159, 365, 392, 1145
AclWI GGATC 4 cut(s) 202, 238, 689, 961
AcuI CTGAAG 1 cut(s) 1178
AcyI GRCGYC 1 cut(s) 603
AdeI CACNNNGTG 2 cut(s) 847, 849
AfaI GTAC 1 cut(s) 356
AfeI AGCGCT 1 cut(s) 1240
AfiI CCNNNNNNNGG 1 cut(s) 1162
AflII CTTAAG 1 cut(s) 803
AgsI TTSAA 3 cut(s) 466, 887, 893
AjnI CCWGG 2 cut(s) 100, 684
AluBI AGCT 7 cut(s) 214, 462, 565, 920, 1020, 1202, 1219
AluI AGCT 7 cut(s) 214, 462, 565, 920, 1020, 1202, 1219
Alw26I GTCTC 6 cut(s) 38, 613, 941, 1032, 1076, 1307
AlwI GGATC 4 cut(s) 202, 238, 689, 961
Ama87I CYCGRG 2 cut(s) 29, 320
Aor51HI AGCGCT 1 cut(s) 1240
ApeKI GCWGC 7 cut(s) 83, 362, 579, 816, 819, 1202, 1219
Asp700I GAANNNNTTC 1 cut(s) 1240
AspLEI GCGC 4 cut(s) 337, 675, 802, 1241
AspS9I GGNCC 2 cut(s) 656, 981
AsuHPI GGTGA 2 cut(s) 893, 1055
AvaI CYCGRG 2 cut(s) 29, 320
AvaII GGWCC 2 cut(s) 656, 981
AxyI CCTNAGG 1 cut(s) 69
BanII GRGCYC 1 cut(s) 238
BauI CACGAG 1 cut(s) 651
BbsI GAAGAC 2 cut(s) 768, 900
BbvI GCAGC 7 cut(s) 70, 374, 566, 828, 831, 1189, 1206
BccI CCATC 7 cut(s) 99, 299, 325, 406, 802, 983, 1069
BceAI ACGGC 3 cut(s) 660, 723, 854
BciT130I CCWGG 2 cut(s) 102, 686
BcoDI GTCTC 6 cut(s) 38, 613, 941, 1032, 1076, 1307
BfaI CTAG 2 cut(s) 53, 663
BfoI RGCGCY 2 cut(s) 803, 1242
BfrI CTTAAG 1 cut(s) 803
BisI GCNGC 7 cut(s) 84, 363, 580, 817, 820, 1203, 1220
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 7 cut(s) 85, 364, 581, 818, 821, 1204, 1221
Bme1390I CCNGG 2 cut(s) 102, 686
Bme18I GGWCC 2 cut(s) 656, 981
BmeT110I CYCGRG 2 cut(s) 29, 320
BmgT120I GGNCC 2 cut(s) 656, 981
BmiI GGNNCC 4 cut(s) 60, 110, 157, 237
BmrFI CCNGG 2 cut(s) 102, 686
BmsI GCATC 3 cut(s) 443, 643, 775
BpiI GAAGAC 2 cut(s) 768, 900
BplI GAGNNNNNCTC 2 cut(s) 610, 642
BpmI CTGGAG 1 cut(s) 668
Bpu1102I GCTNAGC 1 cut(s) 789
BpuEI CTTGAG 2 cut(s) 581, 906
BsaHI GRCGYC 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 2 cut(s) 629, 685
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 4 cut(s) 498, 510, 528, 540
Bsc4I CCNNNNNNNGG 1 cut(s) 1162
Bse118I RCCGGY 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 131
Bse21I CCTNAGG 1 cut(s) 69
Bse3DI GCAATG 2 cut(s) 668, 829
BseBI CCWGG 2 cut(s) 102, 686
BseDI CCNNGG 2 cut(s) 629, 685
BseGI GGATG 6 cut(s) 110, 291, 432, 634, 860, 1080
BseLI CCNNNNNNNGG 1 cut(s) 1162
BseMI GCAATG 2 cut(s) 668, 829
BseMII CTCAG 2 cut(s) 60, 780
BseNI ACTGG 1 cut(s) 131
BseRI GAGGAG 3 cut(s) 86, 519, 522
BseXI GCAGC 7 cut(s) 70, 374, 566, 828, 831, 1189, 1206
Bsh1236I CGCG 1 cut(s) 392
Bsh1285I CGRYCG 1 cut(s) 379
BsiEI CGRYCG 1 cut(s) 379
BsiHKCI CYCGRG 2 cut(s) 29, 320
BsiSI CCGG 2 cut(s) 153, 594
BslI CCNNNNNNNGG 1 cut(s) 1162
BsmAI GTCTC 6 cut(s) 38, 613, 941, 1032, 1076, 1307
Bso31I GGTCTC 1 cut(s) 38
BsoBI CYCGRG 2 cut(s) 29, 320
Bsp1286I GDGCHC 1 cut(s) 238
Bsp143I GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
Bsp1720I GCTNAGC 1 cut(s) 789
BspACI CCGC 5 cut(s) 141, 159, 365, 392, 1145
BspCNI CTCAG 2 cut(s) 61, 781
BspFNI CGCG 1 cut(s) 392
BspLI GGNNCC 4 cut(s) 60, 110, 157, 237
BspPI GGATC 4 cut(s) 202, 238, 689, 961
BspTI CTTAAG 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 668, 829
BsrFI RCCGGY 1 cut(s) 593
BsrI ACTGG 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 593
BssECI CCNNGG 2 cut(s) 629, 685
BssMI GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
BssNI GRCGYC 1 cut(s) 603
BssSI CACGAG 1 cut(s) 651
Bst2BI CACGAG 1 cut(s) 651
Bst2UI CCWGG 2 cut(s) 102, 686
Bst4CI ACNGT 5 cut(s) 49, 767, 847, 935, 985
Bst6I CTCTTC 2 cut(s) 1074, 1305
BstACI GRCGYC 1 cut(s) 603
BstAFI CTTAAG 1 cut(s) 803
BstDEI CTNAG 4 cut(s) 69, 789, 908, 1270
BstDSI CCRYGG 1 cut(s) 629
BstENI CCTNNNNNAGG 1 cut(s) 1160
BstF5I GGATG 6 cut(s) 110, 291, 432, 634, 860, 1080
BstFNI CGCG 1 cut(s) 392
BstH2I RGCGCY 2 cut(s) 803, 1242
BstHHI GCGC 4 cut(s) 337, 675, 802, 1241
BstKTI GATC 7 cut(s) 210, 246, 379, 697, 728, 834, 969
BstMAI GTCTC 6 cut(s) 38, 613, 941, 1032, 1076, 1307
BstMBI GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
BstMCI CGRYCG 1 cut(s) 379
BstMWI GCNNNNNNNGC 5 cut(s) 389, 468, 611, 799, 806
BstNI CCWGG 2 cut(s) 102, 686
BstNSI RCATGY 1 cut(s) 406
BstSCI CCNGG 2 cut(s) 100, 684
BstUI CGCG 1 cut(s) 392
BstV1I GCAGC 7 cut(s) 70, 374, 566, 828, 831, 1189, 1206
BstV2I GAAGAC 2 cut(s) 768, 900
BstX2I RGATCY 1 cut(s) 207
BstYI RGATCY 1 cut(s) 207
Bsu36I CCTNAGG 1 cut(s) 69
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 6 cut(s) 110, 291, 432, 634, 860, 1080
BtsIMutI CAGTG 3 cut(s) 595, 843, 1305
CfoI GCGC 4 cut(s) 337, 675, 802, 1241
Cfr10I RCCGGY 1 cut(s) 593
Cfr13I GGNCC 2 cut(s) 656, 981
CseI GACGC 2 cut(s) 75, 611
Csp6I GTAC 1 cut(s) 355
CspCI CAANNNNNGTGG 2 cut(s) 611, 646
CviAII CATG 5 cut(s) 64, 175, 403, 644, 736
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 4 cut(s) 69, 789, 908, 1270
DpnI GATC 7 cut(s) 209, 245, 378, 696, 727, 833, 968
DpnII GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
DraIII CACNNNGTG 2 cut(s) 847, 849
DrdI GACNNNNNNGTC 1 cut(s) 608
DseDI GACNNNNNNGTC 1 cut(s) 608
Eam1104I CTCTTC 2 cut(s) 1074, 1305
EarI CTCTTC 2 cut(s) 1074, 1305
Eco24I GRGCYC 1 cut(s) 238
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 656, 981
Eco47III AGCGCT 1 cut(s) 1240
Eco57I CTGAAG 1 cut(s) 1178
Eco81I CCTNAGG 1 cut(s) 69
Eco88I CYCGRG 2 cut(s) 29, 320
EcoNI CCTNNNNNAGG 1 cut(s) 1160
EcoRII CCWGG 2 cut(s) 100, 684
EcoT22I ATGCAT 1 cut(s) 737
EcoT38I GRGCYC 1 cut(s) 238
FaeI CATG 5 cut(s) 67, 178, 406, 647, 739
FalI AAGNNNNNCTT 2 cut(s) 1228, 1260
FatI CATG 5 cut(s) 63, 174, 402, 643, 735
Fnu4HI GCNGC 7 cut(s) 84, 363, 580, 817, 820, 1203, 1220
FokI GGATG 6 cut(s) 117, 278, 439, 621, 867, 1087
FriOI GRGCYC 1 cut(s) 238
Fsp4HI GCNGC 7 cut(s) 84, 363, 580, 817, 820, 1203, 1220
FspBI CTAG 2 cut(s) 53, 663
GlaI GCGC 4 cut(s) 336, 674, 801, 1240
GluI GCNGC 7 cut(s) 84, 363, 580, 817, 820, 1203, 1220
GsuI CTGGAG 1 cut(s) 668
HaeII RGCGCY 2 cut(s) 803, 1242
HapII CCGG 2 cut(s) 153, 594
HgaI GACGC 2 cut(s) 75, 611
HhaI GCGC 4 cut(s) 337, 675, 802, 1241
Hin1I GRCGYC 1 cut(s) 603
Hin1II CATG 5 cut(s) 67, 178, 406, 647, 739
Hin6I GCGC 4 cut(s) 335, 673, 800, 1239
HinP1I GCGC 4 cut(s) 335, 673, 800, 1239
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 8 cut(s) 183, 286, 883, 896, 912, 959, 1069, 1295
HpaII CCGG 2 cut(s) 153, 594
HphI GGTGA 2 cut(s) 893, 1055
Hpy188I TCNGA 8 cut(s) 18, 224, 439, 1054, 1074, 1153, 1235, 1300
Hpy188III TCNNGA 6 cut(s) 320, 322, 496, 560, 692, 923
HpyAV CCTTC 6 cut(s) 227, 449, 648, 914, 1156, 1158
HpyCH4III ACNGT 5 cut(s) 49, 767, 847, 935, 985
HpyCH4V TGCA 5 cut(s) 203, 447, 582, 722, 735
HpyF10VI GCNNNNNNNGC 5 cut(s) 389, 468, 611, 799, 806
HpyF3I CTNAG 4 cut(s) 69, 789, 908, 1270
Hsp92I GRCGYC 1 cut(s) 603
Hsp92II CATG 5 cut(s) 67, 178, 406, 647, 739
HspAI GCGC 4 cut(s) 335, 673, 800, 1239
Kzo9I GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
LmnI GCTCC 2 cut(s) 219, 241
Lsp1109I GCAGC 7 cut(s) 70, 374, 566, 828, 831, 1189, 1206
LweI GCATC 3 cut(s) 443, 643, 775
MaeI CTAG 2 cut(s) 53, 663
MaeIII GTNAC 2 cut(s) 599, 1303
MalI GATC 7 cut(s) 209, 245, 378, 696, 727, 833, 968
MboI GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
MfeI CAATTG 1 cut(s) 1275
MflI RGATCY 1 cut(s) 207
MhlI GDGCHC 1 cut(s) 238
MluCI AATT 2 cut(s) 19, 1275
MlyI GAGTC 4 cut(s) 177, 890, 968, 1063
Mph1103I ATGCAT 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 1240
MseI TTAA 4 cut(s) 386, 804, 1110, 1116
MslI CAYNNNNRTG 1 cut(s) 173
MspA1I CMGCKG 1 cut(s) 365
MspCI CTTAAG 1 cut(s) 803
MspI CCGG 2 cut(s) 153, 594
MspR9I CCNGG 2 cut(s) 102, 686
MunI CAATTG 1 cut(s) 1275
MvaI CCWGG 2 cut(s) 102, 686
MvnI CGCG 1 cut(s) 392
MwoI GCNNNNNNNGC 5 cut(s) 389, 468, 611, 799, 806
NdeII GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
NlaIII CATG 5 cut(s) 67, 178, 406, 647, 739
NlaIV GGNNCC 4 cut(s) 60, 110, 157, 237
NmuCI GTSAC 2 cut(s) 599, 1303
NsiI ATGCAT 1 cut(s) 737
NspI RCATGY 1 cut(s) 406
PaeR7I CTCGAG 2 cut(s) 29, 320
PdmI GAANNNNTTC 1 cut(s) 1240
PfeI GAWTC 4 cut(s) 286, 883, 912, 1295
PkrI GCNGC 7 cut(s) 85, 364, 581, 818, 821, 1204, 1221
Ple19I CGATCG 1 cut(s) 379
PleI GAGTC 4 cut(s) 177, 890, 967, 1063
PpsI GAGTC 4 cut(s) 177, 890, 967, 1063
Psp6I CCWGG 2 cut(s) 100, 684
PspGI CCWGG 2 cut(s) 100, 684
PspN4I GGNNCC 4 cut(s) 60, 110, 157, 237
PspPI GGNCC 2 cut(s) 656, 981
PspXI VCTCGAGB 1 cut(s) 29
PsuI RGATCY 1 cut(s) 207
PvuI CGATCG 1 cut(s) 379
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 1 cut(s) 173
SaqAI TTAA 4 cut(s) 386, 804, 1110, 1116
SatI GCNGC 7 cut(s) 84, 363, 580, 817, 820, 1203, 1220
Sau3AI GATC 7 cut(s) 207, 243, 376, 694, 725, 831, 966
Sau96I GGNCC 2 cut(s) 656, 981
SchI GAGTC 4 cut(s) 177, 890, 968, 1063
ScrFI CCNGG 2 cut(s) 102, 686
SduI GDGCHC 1 cut(s) 238
SfaNI GCATC 3 cut(s) 443, 643, 775
Sfr274I CTCGAG 2 cut(s) 29, 320
SinI GGWCC 2 cut(s) 656, 981
SlaI CTCGAG 2 cut(s) 29, 320
SmiMI CAYNNNNRTG 1 cut(s) 173
SmlI CTYRAG 5 cut(s) 29, 320, 560, 803, 921
SmoI CTYRAG 5 cut(s) 29, 320, 560, 803, 921
Sse9I AATT 2 cut(s) 19, 1275
SsiI CCGC 5 cut(s) 141, 159, 365, 392, 1145
SspMI CTAG 2 cut(s) 53, 663
StyD4I CCNGG 2 cut(s) 100, 684
TaaI ACNGT 5 cut(s) 49, 767, 847, 935, 985
TaqI TCGA 6 cut(s) 30, 321, 329, 379, 1064, 1188
TasI AATT 2 cut(s) 19, 1275
TatI WGTACW 1 cut(s) 354
TfiI GAWTC 4 cut(s) 286, 883, 912, 1295
Tru1I TTAA 4 cut(s) 386, 804, 1110, 1116
Tru9I TTAA 4 cut(s) 386, 804, 1110, 1116
TscAI CASTG 3 cut(s) 595, 850, 1312
TseFI GTSAC 2 cut(s) 599, 1303
TseI GCWGC 7 cut(s) 83, 362, 579, 816, 819, 1202, 1219
Tsp45I GTSAC 2 cut(s) 599, 1303
TspDTI ATGAA 6 cut(s) 131, 760, 774, 1188, 1241, 1308
TspGWI ACGGA 1 cut(s) 618
TspRI CASTG 3 cut(s) 595, 850, 1312
Vha464I CTTAAG 1 cut(s) 803
VpaK11BI GGWCC 2 cut(s) 656, 981
XagI CCTNNNNNAGG 1 cut(s) 1160
XceI RCATGY 1 cut(s) 406
XhoI CTCGAG 2 cut(s) 29, 320
XmnI GAANNNNTTC 1 cut(s) 1240
XspI CTAG 2 cut(s) 53, 663
Zsp2I ATGCAT 1 cut(s) 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.