FvH4_7g23730

Protein of unknown function (DUF620)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013405
Physical Location & Seq
Reverse (-)
18448563 .. 18450807
2245 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23730.t3

Sequence Viewer

Length: 1299 bp
ATGAGGAAGCTTTGTCCCAACTTGGACCGTGAAGACGGCTTGGAAACTGTTCTGGAGGTTCCAATCCCAGAAGAGATATTCTCTCCTACTAACAAAAGCCCTAAGATTTCATGGCAGGGCATGAAGTCATGGATGAAATCATCAAACACCGAGCATTCAACTGTATTCGGAAGCAAAAGCGCAGAGATCCAGCTCTTGCTCGGAGTTATTGGAGCACCACTGATTCCTATTACTATTCCCAACAACGATCACCAACTCATCAATCGCAACATCAAGGATCAGCCTATTGAAGCTTCAATGGCTAAATACATTGTGAAGCAATACATAGCTGCAGTGGGTGGGGAGACTGCCTTGAACTCTATAGACAGCATGTATGCAATGGGAAAGGTGAAGATGGCAGCTTCAGAGTTTTGTACTGGGGAAGCAAAGTTAAATAGTCCCAAGAGAAAGATGAAGAACTTGAAATCACTTGAAAATGGTGAAATGGGAGGATTTGTACTATGGCAGAAGAGGCCAGAGCTATGGTGCTTGGAGCTGGTGGTGTCTGGCTGCAAGATTAGCGCCGGGAGTGATGGCAAGGTGGCTTGGCGACAAACCCCCTGGCATCACTCCCACGCTTCTCGAGGCCCGCCTCGTCCCCTCAGACGCCTCCTTCAGGGACTTGATCCAAGGTCAACTGCAAATTTGTTCAGCAATTCAGTTTGTATTGGAGAGAAGCCAATCAACGAAGAAGACTGCTTCATACTTAAAGTGGAAGCCGAACCCTCAACGCTCCGAGCAAGAAGCAGCAGCAACGTAGAGATAATACGACACACCGTTTGGGGATACTTCAGCCAAAGAACAGGTCTCCTAGTCCAACTAGAAGACTCCCACCTCCTGAGAATCAAGACCTCCGGCAACGACAGCATTTTCTGGGAAACCACAATGGAGTCGTACATCCAAGACTACAGAACCATCGACGGCGTTAACATCGCACATTCCGGGAGGACAAGCGTGTCGTTGGTGAGGTTTGGTGAGAGCAGTGAAGCGCATTCGAGGACTAGAATGGAAGAGGTTTGGGACATTGAGGAAGTTGATTTCAACATTAAGGGTTTGTCTATGGACTGCTTCTTGCCTCCTGGTGACTTGAAGACAGAGGAAGAAGGAGGGTGTGGGATGGTGACTAATAATACCAAGGCAAAGTTACCGTTCAGGATTTTATCATCTTCAAAAGTGCGAGCTTCCAAAATAGCCGCCATTGATGTTTATGATGATGATTCAGAGAGCAGTAGTGGAAGTAGTACTGACCAAGAACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF620

Protein Analysis

433

Amino Acids

48.03

Weight (kDa)

6.06

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF620 PF04788 86 - 335 2.3e-126 Protein of unknown function (DUF620)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013405)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05840
fragaria_vesca FvH4_7g23730 FvH4_7g23730 FvH4_7g23730
malus_domestica MD01G1144500.v1.1
prunus_persica Prupe.2G243900_v2.0.a1
pyrus_communis pycom01g16410 pycom07g19460
rosa_chinensis RchiOBHm_Chr1g0369031
rosa_laevigata RLG00000027163
rosa_multiflora Rmu_sc0004250.1_g000029
rosa_roxburghii Rroxscaffold_4G00288070
rosa_rugosa Rorug01G0347000
rosa_samantha Rh1AG354100 Rh1BG317500 Rh1CG332000 Rh1DG348400
rosa_wichuraiana Rw1G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 994
AciI CCGC 2 cut(s) 629, 1233
AclWI GGATC 3 cut(s) 181, 285, 659
AcsI RAATTY 1 cut(s) 682
AcuI CTGAAG 3 cut(s) 387, 638, 814
AcyI GRCGYC 1 cut(s) 646
AfaI GTAC 4 cut(s) 415, 498, 935, 1282
AfiI CCNNNNNNNGG 1 cut(s) 655
AgsI TTSAA 9 cut(s) 159, 290, 297, 355, 463, 473, 1081, 1129, 1209
AjnI CCWGG 2 cut(s) 599, 1117
AluBI AGCT 8 cut(s) 10, 193, 293, 329, 401, 520, 535, 1220
AluI AGCT 8 cut(s) 10, 193, 293, 329, 401, 520, 535, 1220
Alw21I GWGCWC 1 cut(s) 217
Alw26I GTCTC 2 cut(s) 338, 851
AlwI GGATC 3 cut(s) 181, 285, 659
Ama87I CYCGRG 1 cut(s) 621
AoxI GGCC 2 cut(s) 512, 625
ApeKI GCWGC 5 cut(s) 329, 398, 549, 786, 789
ApoI RAATTY 1 cut(s) 682
ArsI GACNNNNNNTTYG 4 cut(s) 801, 829, 833, 861
Asp700I GAANNNNTTC 1 cut(s) 48
AspLEI GCGC 3 cut(s) 182, 563, 1030
AspS9I GGNCC 2 cut(s) 25, 626
AsuC2I CCSGG 2 cut(s) 565, 982
AsuHPI GGTGA 7 cut(s) 242, 400, 491, 1015, 1025, 1133, 1171
AvaI CYCGRG 1 cut(s) 621
AvaII GGWCC 1 cut(s) 25
BbsI GAAGAC 4 cut(s) 39, 738, 870, 1136
Bbv12I GWGCWC 1 cut(s) 217
BbvI GCAGC 5 cut(s) 316, 410, 536, 798, 801
BccI CCATC 4 cut(s) 388, 566, 962, 1150
BceAI ACGGC 2 cut(s) 52, 976
BciT130I CCWGG 2 cut(s) 601, 1119
BciVI GTATCC 1 cut(s) 818
BcnI CCSGG 2 cut(s) 565, 982
BcoDI GTCTC 2 cut(s) 338, 851
BfaI CTAG 3 cut(s) 851, 860, 1041
BfmI CTRYAG 3 cut(s) 330, 360, 946
BfoI RGCGCY 1 cut(s) 564
BfuI GTATCC 1 cut(s) 818
BisI GCNGC 6 cut(s) 330, 399, 550, 787, 790, 1233
BlsI GCNGC 6 cut(s) 331, 400, 551, 788, 791, 1234
BmcAI AGTACT 1 cut(s) 1282
Bme1390I CCNGG 4 cut(s) 565, 601, 982, 1119
Bme18I GGWCC 1 cut(s) 25
BmeT110I CYCGRG 1 cut(s) 621
BmgT120I GGNCC 2 cut(s) 25, 626
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 4 cut(s) 565, 601, 982, 1119
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 613
BmuI ACTGGG 1 cut(s) 426
BpiI GAAGAC 4 cut(s) 39, 738, 870, 1136
BplI GAGNNNNNCTC 2 cut(s) 65, 97
BpmI CTGGAG 1 cut(s) 74
BpuMI CCSGG 2 cut(s) 565, 982
BsaHI GRCGYC 1 cut(s) 646
BsaI GGTCTC 1 cut(s) 851
BsaJI CCNNGG 3 cut(s) 599, 668, 1173
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bsc4I CCNNNNNNNGG 1 cut(s) 655
Bse1I ACTGG 1 cut(s) 421
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 2 cut(s) 601, 1119
BseDI CCNNGG 3 cut(s) 599, 668, 1173
BseGI GGATG 3 cut(s) 138, 936, 1161
BseLI CCNNNNNNNGG 1 cut(s) 655
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 2 cut(s) 655, 869
BseNI ACTGG 1 cut(s) 421
BseXI GCAGC 5 cut(s) 316, 410, 536, 798, 801
BshFI GGCC 2 cut(s) 514, 627
BsiHKAI GWGCWC 1 cut(s) 217
BsiHKCI CYCGRG 1 cut(s) 621
BsiSI CCGG 3 cut(s) 564, 894, 981
BslFI GGGAC 4 cut(s) 423, 621, 672, 1073
BslI CCNNNNNNNGG 1 cut(s) 655
BsmAI GTCTC 2 cut(s) 338, 851
BsmFI GGGAC 4 cut(s) 423, 621, 672, 1073
BsmI GAATGC 2 cut(s) 154, 1030
BsnI GGCC 2 cut(s) 514, 627
Bso31I GGTCTC 1 cut(s) 851
BsoBI CYCGRG 1 cut(s) 621
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 4 cut(s) 186, 247, 277, 664
BspACI CCGC 2 cut(s) 629, 1233
BspANI GGCC 2 cut(s) 514, 627
BspCNI CTCAG 2 cut(s) 654, 870
BspLI GGNNCC 1 cut(s) 60
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 3 cut(s) 181, 285, 659
BspTNI GGTCTC 1 cut(s) 851
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 421
BssECI CCNNGG 3 cut(s) 599, 668, 1173
BssMI GATC 4 cut(s) 186, 247, 277, 664
BssNI GRCGYC 1 cut(s) 646
BssT1I CCWWGG 2 cut(s) 668, 1173
Bst2UI CCWGG 2 cut(s) 601, 1119
Bst4CI ACNGT 6 cut(s) 29, 49, 163, 817, 1188, 1296
Bst6I CTCTTC 3 cut(s) 66, 503, 1044
BstACI GRCGYC 1 cut(s) 646
BstC8I GCNNGC 2 cut(s) 629, 1218
BstDEI CTNAG 3 cut(s) 102, 641, 878
BstENI CCTNNNNNAGG 1 cut(s) 653
BstF5I GGATG 3 cut(s) 138, 936, 1161
BstH2I RGCGCY 1 cut(s) 564
BstHHI GCGC 3 cut(s) 182, 563, 1030
BstKTI GATC 4 cut(s) 189, 250, 280, 667
BstMAI GTCTC 2 cut(s) 338, 851
BstMBI GATC 4 cut(s) 186, 247, 277, 664
BstMWI GCNNNNNNNGC 4 cut(s) 299, 511, 558, 903
BstNI CCWGG 2 cut(s) 601, 1119
BstNSI RCATGY 1 cut(s) 373
BstSCI CCNGG 4 cut(s) 563, 599, 980, 1117
BstSFI CTRYAG 3 cut(s) 330, 360, 946
BstV1I GCAGC 5 cut(s) 316, 410, 536, 798, 801
BstV2I GAAGAC 4 cut(s) 39, 738, 870, 1136
BstX2I RGATCY 1 cut(s) 186
BstXI CCANNNNNNTGG 1 cut(s) 522
BstYI RGATCY 1 cut(s) 186
BsuI GTATCC 1 cut(s) 818
BsuRI GGCC 2 cut(s) 514, 627
BtgZI GCGATG 1 cut(s) 955
BtsCI GGATG 3 cut(s) 138, 936, 1161
BtsI GCAGTG 2 cut(s) 339, 1027
BtsIMutI CAGTG 3 cut(s) 218, 339, 1027
Cac8I GCNNGC 2 cut(s) 629, 1218
CfoI GCGC 3 cut(s) 182, 563, 1030
Cfr13I GGNCC 2 cut(s) 25, 626
CseI GACGC 1 cut(s) 654
Csp6I GTAC 4 cut(s) 414, 497, 934, 1281
CviAII CATG 4 cut(s) 111, 121, 129, 370
CviQI GTAC 4 cut(s) 414, 497, 934, 1281
DdeI CTNAG 3 cut(s) 102, 641, 878
DpnI GATC 4 cut(s) 188, 249, 279, 666
DpnII GATC 4 cut(s) 186, 247, 277, 664
DrdI GACNNNNNNGTC 1 cut(s) 994
DseDI GACNNNNNNGTC 1 cut(s) 994
Eam1104I CTCTTC 3 cut(s) 66, 503, 1044
EarI CTCTTC 3 cut(s) 66, 503, 1044
Eco130I CCWWGG 2 cut(s) 668, 1173
Eco31I GGTCTC 1 cut(s) 851
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 3 cut(s) 387, 638, 814
Eco88I CYCGRG 1 cut(s) 621
EcoNI CCTNNNNNAGG 1 cut(s) 653
EcoRII CCWGG 2 cut(s) 599, 1117
EcoT14I CCWWGG 2 cut(s) 668, 1173
ErhI CCWWGG 2 cut(s) 668, 1173
FaeI CATG 4 cut(s) 114, 124, 132, 373
FaqI GGGAC 4 cut(s) 423, 621, 672, 1073
FatI CATG 4 cut(s) 110, 120, 128, 369
FauI CCCGC 1 cut(s) 636
Fnu4HI GCNGC 6 cut(s) 330, 399, 550, 787, 790, 1233
FokI GGATG 3 cut(s) 145, 923, 1168
Fsp4HI GCNGC 6 cut(s) 330, 399, 550, 787, 790, 1233
FspBI CTAG 3 cut(s) 851, 860, 1041
GlaI GCGC 3 cut(s) 181, 562, 1029
GluI GCNGC 6 cut(s) 330, 399, 550, 787, 790, 1233
GsuI CTGGAG 1 cut(s) 74
HaeII RGCGCY 1 cut(s) 564
HaeIII GGCC 2 cut(s) 514, 627
HapII CCGG 3 cut(s) 564, 894, 981
HgaI GACGC 1 cut(s) 654
HhaI GCGC 3 cut(s) 182, 563, 1030
Hin1I GRCGYC 1 cut(s) 646
Hin1II CATG 4 cut(s) 114, 124, 132, 373
Hin6I GCGC 3 cut(s) 180, 561, 1028
HinP1I GCGC 3 cut(s) 180, 561, 1028
HincII GTYRAC 2 cut(s) 675, 967
HindII GTYRAC 2 cut(s) 675, 967
HindIII AAGCTT 2 cut(s) 8, 291
HinfI GANTC 5 cut(s) 223, 866, 882, 929, 1256
HpaI GTTAAC 1 cut(s) 967
HpaII CCGG 3 cut(s) 564, 894, 981
HphI GGTGA 7 cut(s) 242, 400, 491, 1015, 1025, 1133, 1171
Hpy166II GTNNAC 2 cut(s) 675, 967
Hpy188I TCNGA 6 cut(s) 170, 203, 406, 644, 776, 1261
Hpy188III TCNNGA 5 cut(s) 53, 621, 877, 886, 1192
Hpy8I GTNNAC 2 cut(s) 675, 967
Hpy99I CGWCG 1 cut(s) 962
HpyAV CCTTC 2 cut(s) 662, 1136
HpyCH4III ACNGT 6 cut(s) 29, 49, 163, 817, 1188, 1296
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 4 cut(s) 332, 377, 552, 680
HpyF10VI GCNNNNNNNGC 4 cut(s) 299, 511, 558, 903
HpyF3I CTNAG 3 cut(s) 102, 641, 878
HpySE526I ACGT 1 cut(s) 795
Hsp92I GRCGYC 1 cut(s) 646
Hsp92II CATG 4 cut(s) 114, 124, 132, 373
HspAI GCGC 3 cut(s) 180, 561, 1028
KspAI GTTAAC 1 cut(s) 967
Kzo9I GATC 4 cut(s) 186, 247, 277, 664
LmnI GCTCC 3 cut(s) 212, 532, 777
Lsp1109I GCAGC 5 cut(s) 316, 410, 536, 798, 801
LweI GCATC 1 cut(s) 613
MaeI CTAG 3 cut(s) 851, 860, 1041
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 3 cut(s) 1121, 1159, 1182
MalI GATC 4 cut(s) 188, 249, 279, 666
MboI GATC 4 cut(s) 186, 247, 277, 664
MflI RGATCY 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 2 cut(s) 682, 694
MlyI GAGTC 2 cut(s) 860, 938
MmeI TCCRAC 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 48
MseI TTAA 4 cut(s) 431, 747, 966, 1086
MspI CCGG 3 cut(s) 564, 894, 981
MspR9I CCNGG 4 cut(s) 565, 601, 982, 1119
Mva1269I GAATGC 2 cut(s) 154, 1030
MvaI CCWGG 2 cut(s) 601, 1119
MwoI GCNNNNNNNGC 4 cut(s) 299, 511, 558, 903
NciI CCSGG 2 cut(s) 565, 982
NdeII GATC 4 cut(s) 186, 247, 277, 664
NlaIII CATG 4 cut(s) 114, 124, 132, 373
NlaIV GGNNCC 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 1121, 1159
NspI RCATGY 1 cut(s) 373
PaeR7I CTCGAG 1 cut(s) 621
PctI GAATGC 2 cut(s) 154, 1030
PdmI GAANNNNTTC 1 cut(s) 48
PfeI GAWTC 3 cut(s) 223, 882, 1256
PfoI TCCNGGA 1 cut(s) 980
PkrI GCNGC 6 cut(s) 331, 400, 551, 788, 791, 1234
PleI GAGTC 2 cut(s) 860, 937
PpsI GAGTC 2 cut(s) 860, 937
Psp6I CCWGG 2 cut(s) 599, 1117
PspGI CCWGG 2 cut(s) 599, 1117
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 2 cut(s) 25, 626
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 1 cut(s) 186
RsaI GTAC 4 cut(s) 415, 498, 935, 1282
RsaNI GTAC 4 cut(s) 414, 497, 934, 1281
SaqAI TTAA 4 cut(s) 431, 747, 966, 1086
SatI GCNGC 6 cut(s) 330, 399, 550, 787, 790, 1233
Sau3AI GATC 4 cut(s) 186, 247, 277, 664
Sau96I GGNCC 2 cut(s) 25, 626
ScaI AGTACT 1 cut(s) 1282
SchI GAGTC 2 cut(s) 860, 938
ScrFI CCNGG 4 cut(s) 565, 601, 982, 1119
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 3 cut(s) 330, 360, 946
Sfr274I CTCGAG 1 cut(s) 621
SinI GGWCC 1 cut(s) 25
SlaI CTCGAG 1 cut(s) 621
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
Sse9I AATT 2 cut(s) 682, 694
SsiI CCGC 2 cut(s) 629, 1233
SspMI CTAG 3 cut(s) 851, 860, 1041
StyD4I CCNGG 4 cut(s) 563, 599, 980, 1117
StyI CCWWGG 2 cut(s) 668, 1173
TaaI ACNGT 6 cut(s) 29, 49, 163, 817, 1188, 1296
TaiI ACGT 1 cut(s) 798
TaqI TCGA 3 cut(s) 622, 957, 1034
TasI AATT 2 cut(s) 682, 694
TatI WGTACW 3 cut(s) 413, 496, 1280
TauI GCSGC 1 cut(s) 1235
TfiI GAWTC 3 cut(s) 223, 882, 1256
Tru1I TTAA 4 cut(s) 431, 747, 966, 1086
Tru9I TTAA 4 cut(s) 431, 747, 966, 1086
TscAI CASTG 3 cut(s) 225, 339, 1027
TseFI GTSAC 2 cut(s) 1121, 1159
TseI GCWGC 5 cut(s) 329, 398, 549, 786, 789
Tsp45I GTSAC 2 cut(s) 1121, 1159
TspDTI ATGAA 5 cut(s) 99, 137, 149, 467, 730
TspRI CASTG 3 cut(s) 225, 339, 1027
VpaK11BI GGWCC 1 cut(s) 25
XagI CCTNNNNNAGG 1 cut(s) 653
XapI RAATTY 1 cut(s) 682
XceI RCATGY 1 cut(s) 373
XhoI CTCGAG 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 48
XspI CTAG 3 cut(s) 851, 860, 1041
ZrmI AGTACT 1 cut(s) 1282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.