Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0026685

Physical Location & Seq

Genomic Coordinates Reverse (-)

3960938 .. 3963624

Gene Structure 2687 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G12250.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1350 bp

ATGAGAGAAATCCTTCACATTCAAGGTGGTCAATGTGGGAACCAGATTGGTTCCAAGTTCTGGGAAGTTGTATGTGATGAGCATGGTATTGATCCCACTGGTCGTTACGTTGGAAACTCTGATCTGCAGTTGGAGCGTGTCAATGTTTACTATAACGAGGCATCCTGCGGAAGATATGTTCCCCGTGCAATTCTCATGGATCTTGAGCCTGGTACTATGGACAGTGTCAGAACTGGACCTTATGGTCAAATCTTCAGGCCTGACAACTTTGTTTTCGGGCAATCTGGTGCTGGAAACAACTGGGCTAAAGGGCATTACACTGAAGGAGCTGAGCTTATTGATGCTGTACTCGATGTTGTACGCAAAGAGGCTGAGAATTGCGACTGTCTTCAAGGTTTCCAAGTATGTCACTCACTTGGTGGAGGCACCGGGTCTGGAATGGGAACTCTGCTCATATCTAAGATCAGGGAAGAGTATCCTGATAGAATGATGCTTACATTTTCTGTCTTCCCATCACCAAAGGTCTCTGACACAGTGGTTGAGCCATACAATGCGACACTTTCAGTTCATCAGCTGGTGGAAAATGCTGATGAGTGTATGGTTTTGGACAATGAAGCCCTTTACGACATCTGTTTTAGAACACTTAAGCTTACCACTCCTAGCTTTGGTGATCTGAATCATCTTATCTCTGCAACCATGAGTGGAGTTACATGCTGTCTTAGGTTCCCGGGTCAGCTCAACTCTGATCTGAGGAAGCTCGCAGTGAACCTCATTCCTTTCCCTCGTCTCCACTTTTTCATGGTTGGTTTTGCCCCTCTCACCTCCCGTGGGTCTCAGCAGTACCGTGCACTCACTGTCCCTGAGCTGACCCAACAGATGTGGGATTCAAAGAACATGATGTGCGCAGCAGACCCGCGCCACGGGCGGTACCTAACTGCCTCGGCCATGTTCCGTGGCAAGATGAGCACAAAAGAAGTGGACGAGCAGATGATAAACGTACAGAACAAAAACTCTTCCTACTTTGTGGAATGGATTCCAAACAACGTGAAGTCAAGCGTCTGTGACATAGCTCCCCGAGGTCTCTCAATGGCATCGACATTTATTGGGAATTCCACATCGATCCAAGAGATGTTTAGGCGGGTGAGCGAGCAGTTCACTGCTATGTTCAGGAGGAAAGCTTTCTTGCATTGGTACACAGGTGAAGGAATGGACGAGATGGAGTTTACTGAAGCTGAGAGCAACATGAACGATCTAGTCTCAGAGTACCAGCAATACCAAGACGCAACTGCAGATGACGAAGGCGAGTATGAAGAAGACGAGGATGAAGAAGAGATATTGGATCATGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005200 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005886 GO:0006950 GO:0006970 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009266 GO:0009409 GO:0009628 GO:0009651 GO:0009987 GO:0015630 GO:0016020 GO:0016043 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044424 GO:0044464 GO:0050896 GO:0071840 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04145 ko04540 ko05130 map04145 map04540 map05130

Pfam Domains

Protein Families

Tubulin Tubulin_C

Protein Analysis

449

Amino Acids

50.59

Weight (kDa)

4.72

Isoelectric Point (pI)

36.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Tubulin	PF00091	3 - 211	7.3e-65	Tubulin/FtsZ family, GTPase domain
Tubulin_C	PF03953	261 - 382	1e-39	Tubulin C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0026685)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12250
prunus_persica	Prupe.7G028500_v2.0.a1

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	243
Acc16I	TGCGCA	1 cut(s)	904
Acc65I	GGTACC	1 cut(s)	927
AccB1I	GGYRCC	2 cut(s)	425, 927
AccB7I	CCANNNNNTGG	1 cut(s)	60
AccII	CGCG	1 cut(s)	916
AciI	CCGC	4 cut(s)	168, 914, 925, 1138
AclWI	GGATC	4 cut(s)	86, 207, 1114, 1347
AcoI	YGGCCR	1 cut(s)	942
AcsI	RAATTY	1 cut(s)	1108
AcuI	CTGAAG	3 cut(s)	238, 342, 1248
AdeI	CACNNNGTG	1 cut(s)	419
AfaI	GTAC	8 cut(s)	214, 348, 360, 842, 929, 999, 1193, 1265
AfiI	CCNNNNNNNGG	4 cut(s)	60, 665, 828, 920
AflII	CTTAAG	1 cut(s)	644
AgsI	TTSAA	3 cut(s)	23, 392, 888
AjnI	CCWGG	1 cut(s)	208
AloI	GAACNNNNNNTCC	2 cut(s)	162, 194
Alw21I	GWGCWC	2 cut(s)	850, 968
Alw26I	GTCTC	5 cut(s)	529, 791, 837, 1085, 1261
Alw44I	GTGCAC	1 cut(s)	846
AlwI	GGATC	4 cut(s)	86, 207, 1114, 1347
Ama87I	CYCGRG	2 cut(s)	727, 1074
AoxI	GGCC	2 cut(s)	257, 942
ApaLI	GTGCAC	1 cut(s)	846
ApeKI	GCWGC	1 cut(s)	905
ApoI	RAATTY	1 cut(s)	1108
Asp700I	GAANNNNTTC	3 cut(s)	12, 1032, 1178
Asp718I	GGTACC	1 cut(s)	927
AspLEI	GCGC	2 cut(s)	905, 918
AspS9I	GGNCC	1 cut(s)	236
AsuC2I	CCSGG	3 cut(s)	430, 728, 729
AsuHPI	GGTGA	5 cut(s)	507, 680, 811, 1153, 1211
AvaI	CYCGRG	2 cut(s)	727, 1074
AvaII	GGWCC	1 cut(s)	236
BaeGI	GKGCMC	1 cut(s)	850
BaeI	ACNNNNGTAYC	2 cut(s)	1183, 1216
BanI	GGYRCC	2 cut(s)	425, 927
BbsI	GAAGAC	3 cut(s)	380, 499, 1320
Bbv12I	GWGCWC	2 cut(s)	850, 968
BbvI	GCAGC	1 cut(s)	917
BccI	CCATC	2 cut(s)	520, 1210
BciT130I	CCWGG	1 cut(s)	210
BciVI	GTATCC	1 cut(s)	486
BcnI	CCSGG	3 cut(s)	430, 728, 729
BcoDI	GTCTC	5 cut(s)	529, 791, 837, 1085, 1261
BfaI	CTAG	2 cut(s)	660, 1253
BfmI	CTRYAG	2 cut(s)	125, 1287
BfrI	CTTAAG	1 cut(s)	644
BfuI	GTATCC	1 cut(s)	486
BisI	GCNGC	1 cut(s)	906
BlpI	GCTNAGC	1 cut(s)	330
BlsI	GCNGC	1 cut(s)	907
Bme1390I	CCNGG	4 cut(s)	210, 430, 728, 729
Bme18I	GGWCC	1 cut(s)	236
BmeT110I	CYCGRG	2 cut(s)	727, 1074
BmgT120I	GGNCC	1 cut(s)	236
BmiI	GGNNCC	5 cut(s)	41, 52, 427, 725, 929
BmrFI	CCNGG	4 cut(s)	210, 430, 728, 729
BmrI	ACTGGG	1 cut(s)	310
BmsI	GCATC	4 cut(s)	170, 331, 480, 1100
BmuI	ACTGGG	1 cut(s)	310
BpiI	GAAGAC	3 cut(s)	380, 499, 1320
Bpu10I	CCTNAGC	1 cut(s)	861
Bpu1102I	GCTNAGC	1 cut(s)	330
BpuEI	CTTGAG	1 cut(s)	224
BpuMI	CCSGG	3 cut(s)	430, 728, 729
Bsa29I	ATCGAT	1 cut(s)	1118
BsaBI	GATNNNNATC	1 cut(s)	675
BsaI	GGTCTC	3 cut(s)	529, 837, 1085
BsaJI	CCNNGG	6 cut(s)	727, 826, 919, 939, 952, 1075
Bsc4I	CCNNNNNNNGG	4 cut(s)	60, 665, 828, 920
Bse1I	ACTGG	3 cut(s)	103, 238, 305
Bse8I	GATNNNNATC	1 cut(s)	675
BseBI	CCWGG	1 cut(s)	210
BseCI	ATCGAT	1 cut(s)	1118
BseDI	CCNNGG	6 cut(s)	727, 826, 919, 939, 952, 1075
BseGI	GGATG	2 cut(s)	161, 1327
BseJI	GATNNNNATC	1 cut(s)	675
BseLI	CCNNNNNNNGG	4 cut(s)	60, 665, 828, 920
BseMII	CTCAG	7 cut(s)	321, 363, 740, 848, 852, 1224, 1272
BseNI	ACTGG	3 cut(s)	103, 238, 305
BseSI	GKGCMC	1 cut(s)	850
BseXI	GCAGC	1 cut(s)	917
Bsh1236I	CGCG	1 cut(s)	916
BshFI	GGCC	2 cut(s)	259, 944
BshNI	GGYRCC	2 cut(s)	425, 927
BshVI	ATCGAT	1 cut(s)	1118
BsiHKAI	GWGCWC	2 cut(s)	850, 968
BsiHKCI	CYCGRG	2 cut(s)	727, 1074
BsiSI	CCGG	2 cut(s)	429, 728
BslFI	GGGAC	1 cut(s)	842
BslI	CCNNNNNNNGG	4 cut(s)	60, 665, 828, 920
BsmAI	GTCTC	5 cut(s)	529, 791, 837, 1085, 1261
BsmBI	CGTCTC	1 cut(s)	791
BsmFI	GGGAC	1 cut(s)	842
BsnI	GGCC	2 cut(s)	259, 944
Bso31I	GGTCTC	3 cut(s)	529, 837, 1085
BsoBI	CYCGRG	2 cut(s)	727, 1074
Bsp1286I	GDGCHC	2 cut(s)	850, 968
Bsp143I	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
Bsp1720I	GCTNAGC	1 cut(s)	330
BspACI	CCGC	4 cut(s)	168, 914, 925, 1138
BspANI	GGCC	2 cut(s)	259, 944
BspCNI	CTCAG	7 cut(s)	322, 364, 741, 847, 853, 1225, 1271
BspDI	ATCGAT	1 cut(s)	1118
BspFNI	CGCG	1 cut(s)	916
BspHI	TCATGA	1 cut(s)	1342
BspLI	GGNNCC	5 cut(s)	41, 52, 427, 725, 929
BspMAI	CTGCAG	2 cut(s)	129, 1291
BspPI	GGATC	4 cut(s)	86, 207, 1114, 1347
BspT107I	GGYRCC	2 cut(s)	425, 927
BspTI	CTTAAG	1 cut(s)	644
BspTNI	GGTCTC	3 cut(s)	529, 837, 1085
BsrI	ACTGG	3 cut(s)	103, 238, 305
BssECI	CCNNGG	6 cut(s)	727, 826, 919, 939, 952, 1075
BssMI	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
Bst2UI	CCWGG	1 cut(s)	210
Bst4CI	ACNGT	5 cut(s)	224, 386, 535, 845, 856
Bst6I	CTCTTC	3 cut(s)	465, 1018, 1323
BstAFI	CTTAAG	1 cut(s)	644
BstC8I	GCNNGC	2 cut(s)	759, 1148
BstDEI	CTNAG	9 cut(s)	330, 372, 459, 719, 749, 834, 861, 1233, 1258
BstDSI	CCRYGG	3 cut(s)	826, 919, 952
BstF5I	GGATG	2 cut(s)	161, 1327
BstFNI	CGCG	1 cut(s)	916
BstHHI	GCGC	2 cut(s)	905, 918
BstKTI	GATC	9 cut(s)	94, 124, 202, 465, 673, 748, 1122, 1252, 1342
BstMAI	GTCTC	5 cut(s)	529, 791, 837, 1085, 1261
BstMBI	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
BstMWI	GCNNNNNNNGC	3 cut(s)	133, 922, 963
BstNI	CCWGG	1 cut(s)	210
BstNSI	RCATGY	1 cut(s)	714
BstSCI	CCNGG	4 cut(s)	208, 428, 726, 727
BstSFI	CTRYAG	2 cut(s)	125, 1287
BstSLI	GKGCMC	1 cut(s)	850
BstUI	CGCG	1 cut(s)	916
BstV1I	GCAGC	1 cut(s)	917
BstV2I	GAAGAC	3 cut(s)	380, 499, 1320
BstX2I	RGATCY	1 cut(s)	199
BstYI	RGATCY	1 cut(s)	199
Bsu15I	ATCGAT	1 cut(s)	1118
BsuI	GTATCC	1 cut(s)	486
BsuRI	GGCC	2 cut(s)	259, 944
BsuTUI	ATCGAT	1 cut(s)	1118
BtgI	CCRYGG	3 cut(s)	826, 919, 952
BtsCI	GGATG	2 cut(s)	161, 1327
BtsI	GCAGTG	2 cut(s)	768, 1155
BtsIMutI	CAGTG	7 cut(s)	96, 229, 318, 540, 768, 852, 1155
Cac8I	GCNNGC	2 cut(s)	759, 1148
CciI	TCATGA	1 cut(s)	1342
CfoI	GCGC	2 cut(s)	905, 918
Cfr13I	GGNCC	1 cut(s)	236
Cfr9I	CCCGGG	1 cut(s)	727
ClaI	ATCGAT	1 cut(s)	1118
CseI	GACGC	2 cut(s)	1045, 1289
Csp6I	GTAC	8 cut(s)	213, 347, 359, 841, 928, 998, 1192, 1264
CspCI	CAANNNNNGTGG	4 cut(s)	860, 895, 957, 992
CviAII	CATG	9 cut(s)	83, 196, 697, 711, 799, 895, 946, 1243, 1343
CviQI	GTAC	8 cut(s)	213, 347, 359, 841, 928, 998, 1192, 1264
DdeI	CTNAG	9 cut(s)	330, 372, 459, 719, 749, 834, 861, 1233, 1258
DpnI	GATC	9 cut(s)	93, 123, 201, 464, 672, 747, 1121, 1251, 1341
DpnII	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
DraIII	CACNNNGTG	1 cut(s)	419
DrdI	GACNNNNNNGTC	1 cut(s)	243
DseDI	GACNNNNNNGTC	1 cut(s)	243
EaeI	YGGCCR	1 cut(s)	942
Eam1104I	CTCTTC	3 cut(s)	465, 1018, 1323
EarI	CTCTTC	3 cut(s)	465, 1018, 1323
Eco147I	AGGCCT	1 cut(s)	259
Eco31I	GGTCTC	3 cut(s)	529, 837, 1085
Eco47I	GGWCC	1 cut(s)	236
Eco57I	CTGAAG	3 cut(s)	238, 342, 1248
Eco88I	CYCGRG	2 cut(s)	727, 1074
EcoRI	GAATTC	1 cut(s)	1108
EcoRII	CCWGG	1 cut(s)	208
Esp3I	CGTCTC	1 cut(s)	791
FaeI	CATG	9 cut(s)	86, 199, 700, 714, 802, 898, 949, 1246, 1346
FaqI	GGGAC	1 cut(s)	842
FatI	CATG	9 cut(s)	82, 195, 696, 710, 798, 894, 945, 1242, 1342
FauI	CCCGC	2 cut(s)	921, 1131
Fnu4HI	GCNGC	1 cut(s)	906
FokI	GGATG	2 cut(s)	148, 1334
Fsp4HI	GCNGC	1 cut(s)	906
FspBI	CTAG	2 cut(s)	660, 1253
FspI	TGCGCA	1 cut(s)	904
GlaI	GCGC	2 cut(s)	904, 917
GluI	GCNGC	1 cut(s)	906
HaeIII	GGCC	2 cut(s)	259, 944
HapII	CCGG	2 cut(s)	429, 728
HgaI	GACGC	2 cut(s)	1045, 1289
HhaI	GCGC	2 cut(s)	905, 918
Hin1II	CATG	9 cut(s)	86, 199, 700, 714, 802, 898, 949, 1246, 1346
Hin6I	GCGC	2 cut(s)	903, 916
HinP1I	GCGC	2 cut(s)	903, 916
HindIII	AAGCTT	2 cut(s)	647, 1176
HinfI	GANTC	3 cut(s)	676, 884, 1033
HpaII	CCGG	2 cut(s)	429, 728
HphI	GGTGA	5 cut(s)	507, 680, 811, 1153, 1211
Hpy166II	GTNNAC	7 cut(s)	148, 766, 848, 979, 1155, 1194, 1224
Hpy188I	TCNGA	7 cut(s)	121, 230, 529, 675, 745, 750, 1261
Hpy188III	TCNNGA	5 cut(s)	203, 435, 479, 1168, 1343
Hpy8I	GTNNAC	7 cut(s)	148, 766, 848, 979, 1155, 1194, 1224
HpyAV	CCTTC	4 cut(s)	23, 317, 1196, 1292
HpyCH4III	ACNGT	5 cut(s)	224, 386, 535, 845, 856
HpyCH4IV	ACGT	3 cut(s)	108, 996, 1044
HpyCH4V	TGCA	6 cut(s)	127, 188, 692, 848, 1186, 1289
HpyF10VI	GCNNNNNNNGC	3 cut(s)	133, 922, 963
HpyF3I	CTNAG	9 cut(s)	330, 372, 459, 719, 749, 834, 861, 1233, 1258
HpySE526I	ACGT	3 cut(s)	108, 996, 1044
Hsp92II	CATG	9 cut(s)	86, 199, 700, 714, 802, 898, 949, 1246, 1346
HspAI	GCGC	2 cut(s)	903, 916
KpnI	GGTACC	1 cut(s)	931
Kzo9I	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
LmnI	GCTCC	3 cut(s)	133, 326, 1075
Lsp1109I	GCAGC	1 cut(s)	917
LweI	GCATC	4 cut(s)	170, 331, 480, 1100
MaeI	CTAG	2 cut(s)	660, 1253
MaeII	ACGT	3 cut(s)	108, 996, 1044
MaeIII	GTNAC	4 cut(s)	104, 407, 706, 1061
MalI	GATC	9 cut(s)	93, 123, 201, 464, 672, 747, 1121, 1251, 1341
MboI	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
MflI	RGATCY	1 cut(s)	199
MhlI	GDGCHC	2 cut(s)	850, 968
MluCI	AATT	3 cut(s)	189, 376, 1108
MmeI	TCCRAC	2 cut(s)	91, 111
MroXI	GAANNNNTTC	3 cut(s)	12, 1032, 1178
MseI	TTAA	1 cut(s)	645
MslI	CAYNNNNRTG	1 cut(s)	1160
MspA1I	CMGCKG	1 cut(s)	574
MspCI	CTTAAG	1 cut(s)	644
MspI	CCGG	2 cut(s)	429, 728
MspR9I	CCNGG	4 cut(s)	210, 430, 728, 729
MvaI	CCWGG	1 cut(s)	210
MvnI	CGCG	1 cut(s)	916
MwoI	GCNNNNNNNGC	3 cut(s)	133, 922, 963
NciI	CCSGG	3 cut(s)	430, 728, 729
NdeII	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
NlaIII	CATG	9 cut(s)	86, 199, 700, 714, 802, 898, 949, 1246, 1346
NlaIV	GGNNCC	5 cut(s)	41, 52, 427, 725, 929
NmeAIII	GCCGAG	1 cut(s)	920
NmuCI	GTSAC	2 cut(s)	407, 1061
NsbI	TGCGCA	1 cut(s)	904
NspI	RCATGY	1 cut(s)	714
PagI	TCATGA	1 cut(s)	1342
PceI	AGGCCT	1 cut(s)	259
PdmI	GAANNNNTTC	3 cut(s)	12, 1032, 1178
PfeI	GAWTC	3 cut(s)	676, 884, 1033
PflFI	GACNNNGTC	1 cut(s)	224
PflMI	CCANNNNNTGG	1 cut(s)	60
PkrI	GCNGC	1 cut(s)	907
Psp6I	CCWGG	1 cut(s)	208
PspGI	CCWGG	1 cut(s)	208
PspN4I	GGNNCC	5 cut(s)	41, 52, 427, 725, 929
PspPI	GGNCC	1 cut(s)	236
PstI	CTGCAG	2 cut(s)	129, 1291
PsuI	RGATCY	1 cut(s)	199
PsyI	GACNNNGTC	1 cut(s)	224
PvuII	CAGCTG	1 cut(s)	574
RsaI	GTAC	8 cut(s)	214, 348, 360, 842, 929, 999, 1193, 1265
RsaNI	GTAC	8 cut(s)	213, 347, 359, 841, 928, 998, 1192, 1264
RseI	CAYNNNNRTG	1 cut(s)	1160
SaqAI	TTAA	1 cut(s)	645
SatI	GCNGC	1 cut(s)	906
Sau3AI	GATC	9 cut(s)	91, 121, 199, 462, 670, 745, 1119, 1249, 1339
Sau96I	GGNCC	1 cut(s)	236
ScrFI	CCNGG	4 cut(s)	210, 430, 728, 729
SduI	GDGCHC	2 cut(s)	850, 968
SfaNI	GCATC	4 cut(s)	170, 331, 480, 1100
SfcI	CTRYAG	2 cut(s)	125, 1287
SinI	GGWCC	1 cut(s)	236
SmaI	CCCGGG	1 cut(s)	729
SmiMI	CAYNNNNRTG	1 cut(s)	1160
SmlI	CTYRAG	2 cut(s)	203, 644
SmoI	CTYRAG	2 cut(s)	203, 644
Sse9I	AATT	3 cut(s)	189, 376, 1108
SseBI	AGGCCT	1 cut(s)	259
SsiI	CCGC	4 cut(s)	168, 914, 925, 1138
SspMI	CTAG	2 cut(s)	660, 1253
StuI	AGGCCT	1 cut(s)	259
StyD4I	CCNGG	4 cut(s)	208, 428, 726, 727
TaaI	ACNGT	5 cut(s)	224, 386, 535, 845, 856
TaiI	ACGT	3 cut(s)	111, 999, 1047
TaqI	TCGA	3 cut(s)	351, 1094, 1118
TasI	AATT	3 cut(s)	189, 376, 1108
TatI	WGTACW	1 cut(s)	346
TfiI	GAWTC	3 cut(s)	676, 884, 1033
Tru1I	TTAA	1 cut(s)	645
Tru9I	TTAA	1 cut(s)	645
TscAI	CASTG	7 cut(s)	103, 229, 325, 540, 768, 859, 1162
TseFI	GTSAC	2 cut(s)	407, 1061
TseI	GCWGC	1 cut(s)	905
Tsp45I	GTSAC	2 cut(s)	407, 1061
TspDTI	ATGAA	6 cut(s)	557, 627, 787, 1259, 1323, 1338
TspGWI	ACGGA	1 cut(s)	941
TspMI	CCCGGG	1 cut(s)	727
TspRI	CASTG	7 cut(s)	103, 229, 325, 540, 768, 859, 1162
Tth111I	GACNNNGTC	1 cut(s)	224
Van91I	CCANNNNNTGG	1 cut(s)	60
Vha464I	CTTAAG	1 cut(s)	644
VneI	GTGCAC	1 cut(s)	846
VpaK11BI	GGWCC	1 cut(s)	236
XapI	RAATTY	1 cut(s)	1108
XceI	RCATGY	1 cut(s)	714
XmaI	CCCGGG	1 cut(s)	727
XmnI	GAANNNNTTC	3 cut(s)	12, 1032, 1178
XspI	CTAG	2 cut(s)	660, 1253

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.