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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 58 |
| AciI | CCGC | 2 cut(s) | 47, 94 |
| AclWI | GGATC | 3 cut(s) | 49, 84, 104 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 58 |
| AlwI | GGATC | 3 cut(s) | 49, 84, 104 |
| AoxI | GGCC | 2 cut(s) | 49, 61 |
| AspS9I | GGNCC | 2 cut(s) | 49, 61 |
| BanII | GRGCYC | 1 cut(s) | 105 |
| BfaI | CTAG | 1 cut(s) | 11 |
| BmgT120I | GGNCC | 2 cut(s) | 49, 61 |
| BsaBI | GATNNNNATC | 1 cut(s) | 75 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 58 |
| Bse8I | GATNNNNATC | 1 cut(s) | 75 |
| BseJI | GATNNNNATC | 1 cut(s) | 75 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 58 |
| BshFI | GGCC | 2 cut(s) | 51, 63 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 58 |
| BsnI | GGCC | 2 cut(s) | 51, 63 |
| Bsp1286I | GDGCHC | 1 cut(s) | 105 |
| Bsp143I | GATC | 4 cut(s) | 19, 41, 76, 96 |
| BspACI | CCGC | 2 cut(s) | 47, 94 |
| BspANI | GGCC | 2 cut(s) | 51, 63 |
| BspPI | GGATC | 3 cut(s) | 49, 84, 104 |
| BspQI | GCTCTTC | 1 cut(s) | 12 |
| BssMI | GATC | 4 cut(s) | 19, 41, 76, 96 |
| Bst6I | CTCTTC | 1 cut(s) | 12 |
| BstC8I | GCNNGC | 1 cut(s) | 49 |
| BstKTI | GATC | 4 cut(s) | 22, 44, 79, 99 |
| BstMBI | GATC | 4 cut(s) | 19, 41, 76, 96 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 11, 100 |
| BsuRI | GGCC | 2 cut(s) | 51, 63 |
| Cac8I | GCNNGC | 1 cut(s) | 49 |
| Cfr13I | GGNCC | 2 cut(s) | 49, 61 |
| CviAII | CATG | 1 cut(s) | 38 |
| CviJI | RGCY | 5 cut(s) | 5, 14, 51, 63, 103 |
| CviKI_1 | RGCY | 5 cut(s) | 5, 14, 51, 63, 103 |
| DpnI | GATC | 4 cut(s) | 21, 43, 78, 98 |
| DpnII | GATC | 4 cut(s) | 19, 41, 76, 96 |
| Eam1104I | CTCTTC | 1 cut(s) | 12 |
| EarI | CTCTTC | 1 cut(s) | 12 |
| Eco24I | GRGCYC | 1 cut(s) | 105 |
| EcoT38I | GRGCYC | 1 cut(s) | 105 |
| FaeI | CATG | 1 cut(s) | 41 |
| FaiI | YATR | 3 cut(s) | 29, 39, 90 |
| FatI | CATG | 1 cut(s) | 37 |
| FauI | CCCGC | 1 cut(s) | 40 |
| FriOI | GRGCYC | 1 cut(s) | 105 |
| FspBI | CTAG | 1 cut(s) | 11 |
| HaeIII | GGCC | 2 cut(s) | 51, 63 |
| Hin1II | CATG | 1 cut(s) | 41 |
| Hpy188I | TCNGA | 1 cut(s) | 107 |
| Hpy188III | TCNNGA | 1 cut(s) | 23 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 11, 100 |
| Hsp92II | CATG | 1 cut(s) | 41 |
| Kzo9I | GATC | 4 cut(s) | 19, 41, 76, 96 |
| LguI | GCTCTTC | 1 cut(s) | 12 |
| MaeI | CTAG | 1 cut(s) | 11 |
| MalI | GATC | 4 cut(s) | 21, 43, 78, 98 |
| MboI | GATC | 4 cut(s) | 19, 41, 76, 96 |
| MhlI | GDGCHC | 1 cut(s) | 105 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 11, 100 |
| NdeII | GATC | 4 cut(s) | 19, 41, 76, 96 |
| NlaIII | CATG | 1 cut(s) | 41 |
| PciSI | GCTCTTC | 1 cut(s) | 12 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 58 |
| PspPI | GGNCC | 2 cut(s) | 49, 61 |
| SapI | GCTCTTC | 1 cut(s) | 12 |
| Sau3AI | GATC | 4 cut(s) | 19, 41, 76, 96 |
| Sau96I | GGNCC | 2 cut(s) | 49, 61 |
| SduI | GDGCHC | 1 cut(s) | 105 |
| SgeI | CNNG | 6 cut(s) | 23, 35, 50, 60, 65, 92 |
| SsiI | CCGC | 2 cut(s) | 47, 94 |
| SspMI | CTAG | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 24 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 58 |
| XspI | CTAG | 1 cut(s) | 11 |