AT5G60080
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0026978
Physical Location & Seq
Reverse (-)
24193181 .. 24194909
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60080.1

Sequence Viewer

Length: 1134 bp
ATGAGTAATCGTTTCTTATGCTGTTTAGGAGGCGGAGGCTCAAGTAAAGTAATGCAAGATCCATCGGAACTACCGCAACAACAACAACCATCGGATTCGTCGGATTCATCGGATACACCGGGACAACAAAGTGAAGGTGACGACGAAGCTGACGACGAAGATCCGAGTTCTATAGTTAAATTTCGATGGAATGATGTCGTAGAGGGAACCGATAACTTCGCGATAACACATTTGATCGGCCAAGGAACCTACGGCAAAGTCTATCGCTGCAAGTTCCCAGAAGGTCACAAGGTTGGAGCTGCAAAGATTCATAGTAATGGTATCACAGATGGTCTTAGTGAGACCGTAGCAGAGACTACAACGTTATATGCAGCTGATCACCCAAATGTGATTAAACTTGTCGGCAAATATTATGGGATGGAAAAAAGTGTCCTAGTCTACGAATTCATGCCTAATGGCTCTCTTGACCACCATCTCTTTGCTCACGCGAGGCAGGTTCAGGGTCTTACTCTGCCAACTAGGGTTTTGGATTGGAACACGAGGTTGAGAATTGCTGTTGGTGTAGCCGAAGGTCTTGTTTATGTACACCAGGGGCTTTATGCGATTCACAGAGATATCAAAGTCGAAAACATTTTACTTGACAAGGATTTTGTGCCGAAGCTGACTGATTTTGGATTTGCGACTAAGATCGTTTACAATAGTGATGGTGTTGAGAGGCTGAGGGAGTTCAATACGCGTGGAACTCAAGGATACATAGCACCGGAAGCAGATGAGTTCGCGTTGGTTTCGACTAAGTCCGATATCTATAGCTATGGAGTTCTTCTGTTAGTGCTTTTAACTGGAAGGAAAGCCTATGATCTGGCGAGACCTGTTGCGAAAGAGAAACTCACTGATTGGTTAATGCCAGTATGGACTAGATTGGAGTATGCGCCTATGATAGTTGATGTTGCGCTCGGTAATAAGTATTCAATTGAAGGTTTAAACAGACTACTTCAAACTGCGAGGATGTGTATAAACCCACAGGCACTTGAACGGCCTGCCATGGATTTTGTGGAAACGATGGTTCGTGAAGCTGCAGCTTTCCCGGTCCCAAAGGAGCCCCTGGTGAAAGAGAGGATTTCGACTTCGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

377

Amino Acids

41.9

Weight (kDa)

5.46

Isoelectric Point (pI)

33.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 75 - 287 4.1e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 75 - 286 7.4e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60080 AT5G60090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 484
AccI GTMKAC 1 cut(s) 438
AccII CGCG 4 cut(s) 221, 488, 736, 779
AciI CCGC 2 cut(s) 33, 74
AclI AACGTT 1 cut(s) 362
AclWI GGATC 2 cut(s) 53, 155
AcoI YGGCCR 1 cut(s) 238
AcsI RAATTY 2 cut(s) 179, 443
AfaI GTAC 1 cut(s) 585
AflIII ACRYGT 1 cut(s) 734
AgsI TTSAA 5 cut(s) 730, 969, 974, 995, 1031
AjnI CCWGG 2 cut(s) 588, 1101
AluBI AGCT 7 cut(s) 149, 299, 374, 661, 810, 1073, 1079
AluI AGCT 7 cut(s) 149, 299, 374, 661, 810, 1073, 1079
Alw26I GTCTC 3 cut(s) 335, 347, 859
AlwI GGATC 2 cut(s) 53, 155
AoxI GGCC 2 cut(s) 238, 1034
ApeKI GCWGC 5 cut(s) 267, 299, 371, 1073, 1076
ApoI RAATTY 2 cut(s) 179, 443
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 2 cut(s) 931, 952
AspS9I GGNCC 1 cut(s) 1087
AsuC2I CCSGG 2 cut(s) 120, 1085
AsuHPI GGTGA 3 cut(s) 149, 371, 1117
AsuII TTCGAA 1 cut(s) 1127
AvaII GGWCC 1 cut(s) 1087
BanII GRGCYC 1 cut(s) 1101
BauI CACGAG 1 cut(s) 538
BbvCI CCTCAGC 1 cut(s) 719
BbvI GCAGC 5 cut(s) 254, 286, 383, 1060, 1088
BccI CCATC 8 cut(s) 70, 97, 180, 323, 412, 480, 698, 1054
BceAI ACGGC 2 cut(s) 268, 1049
BciT130I CCWGG 2 cut(s) 590, 1103
BciVI GTATCC 2 cut(s) 106, 743
BclI TGATCA 1 cut(s) 376
BcnI CCSGG 2 cut(s) 120, 1085
BcoDI GTCTC 3 cut(s) 335, 347, 859
BfaI CTAG 3 cut(s) 434, 519, 915
BfmI CTRYAG 3 cut(s) 171, 805, 1074
BfuAI ACCTGC 1 cut(s) 484
BfuI GTATCC 2 cut(s) 106, 743
BisI GCNGC 5 cut(s) 268, 300, 372, 1074, 1077
BlsI GCNGC 5 cut(s) 269, 301, 373, 1075, 1078
Bme1390I CCNGG 4 cut(s) 120, 590, 1085, 1103
Bme18I GGWCC 1 cut(s) 1087
BmgT120I GGNCC 1 cut(s) 1087
BmiI GGNNCC 4 cut(s) 208, 247, 1089, 1098
BmrFI CCNGG 4 cut(s) 120, 590, 1085, 1103
Bpu10I CCTNAGC 1 cut(s) 719
Bpu14I TTCGAA 1 cut(s) 1127
BpuEI CTTGAG 2 cut(s) 25, 729
BpuMI CCSGG 2 cut(s) 120, 1085
BsaI GGTCTC 2 cut(s) 335, 859
BsaJI CCNNGG 4 cut(s) 241, 589, 1041, 1101
BsaWI WCCGGW 1 cut(s) 760
Bse1I ACTGG 2 cut(s) 844, 905
BseBI CCWGG 2 cut(s) 590, 1103
BseDI CCNNGG 4 cut(s) 241, 589, 1041, 1101
BseGI GGATG 2 cut(s) 423, 1011
BseMII CTCAG 1 cut(s) 710
BseNI ACTGG 2 cut(s) 844, 905
BseXI GCAGC 5 cut(s) 254, 286, 383, 1060, 1088
Bsh1236I CGCG 4 cut(s) 221, 488, 736, 779
BshFI GGCC 2 cut(s) 240, 1036
BsiSI CCGG 3 cut(s) 119, 761, 1085
BslFI GGGAC 2 cut(s) 135, 1073
BsmAI GTCTC 3 cut(s) 335, 347, 859
BsmFI GGGAC 2 cut(s) 135, 1073
BsnI GGCC 2 cut(s) 240, 1036
Bso31I GGTCTC 2 cut(s) 335, 859
Bsp119I TTCGAA 1 cut(s) 1127
Bsp1286I GDGCHC 1 cut(s) 1101
Bsp1407I TGTACA 1 cut(s) 583
Bsp143I GATC 6 cut(s) 58, 160, 234, 376, 687, 856
Bsp19I CCATGG 1 cut(s) 1041
Bsp68I TCGCGA 1 cut(s) 221
BspACI CCGC 2 cut(s) 33, 74
BspANI GGCC 2 cut(s) 240, 1036
BspCNI CTCAG 1 cut(s) 711
BspFNI CGCG 4 cut(s) 221, 488, 736, 779
BspLI GGNNCC 4 cut(s) 208, 247, 1089, 1098
BspMAI CTGCAG 1 cut(s) 1078
BspMI ACCTGC 1 cut(s) 484
BspPI GGATC 2 cut(s) 53, 155
BspT104I TTCGAA 1 cut(s) 1127
BspTNI GGTCTC 2 cut(s) 335, 859
BsrGI TGTACA 1 cut(s) 583
BsrI ACTGG 2 cut(s) 844, 905
BssECI CCNNGG 4 cut(s) 241, 589, 1041, 1101
BssMI GATC 6 cut(s) 58, 160, 234, 376, 687, 856
BssSI CACGAG 1 cut(s) 538
BssT1I CCWWGG 2 cut(s) 241, 1041
Bst2BI CACGAG 1 cut(s) 538
Bst2UI CCWGG 2 cut(s) 590, 1103
Bst4CI ACNGT 1 cut(s) 346
BstAUI TGTACA 1 cut(s) 583
BstBI TTCGAA 1 cut(s) 1127
BstC8I GCNNGC 1 cut(s) 1038
BstDEI CTNAG 4 cut(s) 335, 684, 719, 792
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 2 cut(s) 423, 1011
BstFNI CGCG 4 cut(s) 221, 488, 736, 779
BstHHI GCGC 2 cut(s) 931, 952
BstKTI GATC 6 cut(s) 61, 163, 237, 379, 690, 859
BstMAI GTCTC 3 cut(s) 335, 347, 859
BstMBI GATC 6 cut(s) 58, 160, 234, 376, 687, 856
BstMWI GCNNNNNNNGC 1 cut(s) 764
BstNI CCWGG 2 cut(s) 590, 1103
BstSCI CCNGG 4 cut(s) 118, 588, 1083, 1101
BstSFI CTRYAG 3 cut(s) 171, 805, 1074
BstUI CGCG 4 cut(s) 221, 488, 736, 779
BstV1I GCAGC 5 cut(s) 254, 286, 383, 1060, 1088
BstX2I RGATCY 2 cut(s) 58, 160
BstYI RGATCY 2 cut(s) 58, 160
BsuI GTATCC 2 cut(s) 106, 743
BsuRI GGCC 2 cut(s) 240, 1036
BtgI CCRYGG 1 cut(s) 1041
BtsCI GGATG 2 cut(s) 423, 1011
BtsIMutI CAGTG 1 cut(s) 888
BtuMI TCGCGA 1 cut(s) 221
BveI ACCTGC 1 cut(s) 484
Cac8I GCNNGC 1 cut(s) 1038
CfoI GCGC 2 cut(s) 931, 952
Cfr13I GGNCC 1 cut(s) 1087
Csp6I GTAC 1 cut(s) 584
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 2 cut(s) 448, 1042
CviQI GTAC 1 cut(s) 584
DdeI CTNAG 4 cut(s) 335, 684, 719, 792
DpnI GATC 6 cut(s) 60, 162, 236, 378, 689, 858
DpnII GATC 6 cut(s) 58, 160, 234, 376, 687, 856
DraI TTTAAA 1 cut(s) 981
EaeI YGGCCR 1 cut(s) 238
EciI GGCGGA 1 cut(s) 48
Eco130I CCWWGG 2 cut(s) 241, 1041
Eco24I GRGCYC 1 cut(s) 1101
Eco31I GGTCTC 2 cut(s) 335, 859
Eco32I GATATC 2 cut(s) 616, 802
Eco47I GGWCC 1 cut(s) 1087
EcoRI GAATTC 1 cut(s) 443
EcoRII CCWGG 2 cut(s) 588, 1101
EcoRV GATATC 2 cut(s) 616, 802
EcoT14I CCWWGG 2 cut(s) 241, 1041
EcoT38I GRGCYC 1 cut(s) 1101
ErhI CCWWGG 2 cut(s) 241, 1041
FaeI CATG 2 cut(s) 451, 1045
FaqI GGGAC 2 cut(s) 135, 1073
FatI CATG 2 cut(s) 447, 1041
FbaI TGATCA 1 cut(s) 376
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 5 cut(s) 268, 300, 372, 1074, 1077
FokI GGATG 2 cut(s) 430, 1018
FriOI GRGCYC 1 cut(s) 1101
Fsp4HI GCNGC 5 cut(s) 268, 300, 372, 1074, 1077
FspBI CTAG 3 cut(s) 434, 519, 915
GlaI GCGC 2 cut(s) 930, 951
GluI GCNGC 5 cut(s) 268, 300, 372, 1074, 1077
HaeIII GGCC 2 cut(s) 240, 1036
HapII CCGG 3 cut(s) 119, 761, 1085
HhaI GCGC 2 cut(s) 931, 952
Hin1II CATG 2 cut(s) 451, 1045
Hin6I GCGC 2 cut(s) 929, 950
HinP1I GCGC 2 cut(s) 929, 950
HinfI GANTC 4 cut(s) 95, 104, 307, 604
HpaII CCGG 3 cut(s) 119, 761, 1085
HphI GGTGA 3 cut(s) 149, 371, 1117
Hpy166II GTNNAC 3 cut(s) 439, 586, 694
Hpy188I TCNGA 6 cut(s) 67, 94, 103, 112, 165, 799
Hpy188III TCNNGA 3 cut(s) 220, 464, 1067
Hpy8I GTNNAC 3 cut(s) 439, 586, 694
Hpy99I CGWCG 3 cut(s) 103, 146, 158
HpyAV CCTTC 5 cut(s) 128, 275, 563, 837, 968
HpyCH4III ACNGT 1 cut(s) 346
HpyCH4IV ACGT 1 cut(s) 362
HpyCH4V TGCA 5 cut(s) 55, 270, 302, 371, 1076
HpyF10VI GCNNNNNNNGC 1 cut(s) 764
HpyF3I CTNAG 4 cut(s) 335, 684, 719, 792
HpySE526I ACGT 1 cut(s) 362
Hsp92II CATG 2 cut(s) 451, 1045
HspAI GCGC 2 cut(s) 929, 950
Ksp22I TGATCA 1 cut(s) 376
Kzo9I GATC 6 cut(s) 58, 160, 234, 376, 687, 856
LmnI GCTCC 2 cut(s) 296, 1096
Lsp1109I GCAGC 5 cut(s) 254, 286, 383, 1060, 1088
MaeI CTAG 3 cut(s) 434, 519, 915
MaeII ACGT 1 cut(s) 362
MaeIII GTNAC 2 cut(s) 137, 284
MalI GATC 6 cut(s) 60, 162, 236, 378, 689, 858
MboI GATC 6 cut(s) 58, 160, 234, 376, 687, 856
MboII GAAGA 2 cut(s) 170, 812
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 2 cut(s) 58, 160
MhlI GDGCHC 1 cut(s) 1101
MluCI AATT 4 cut(s) 179, 443, 549, 969
MluI ACGCGT 1 cut(s) 734
MmeI TCCRAC 2 cut(s) 81, 274
MnlI CCTC 9 cut(s) 23, 29, 196, 483, 534, 708, 714, 996, 1107
MseI TTAA 5 cut(s) 177, 393, 836, 899, 980
MslI CAYNNNNRTG 2 cut(s) 315, 384
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 3 cut(s) 119, 761, 1085
MspR9I CCNGG 4 cut(s) 120, 590, 1085, 1103
MssI GTTTAAAC 1 cut(s) 981
MunI CAATTG 1 cut(s) 969
MvaI CCWGG 2 cut(s) 590, 1103
MvnI CGCG 4 cut(s) 221, 488, 736, 779
MwoI GCNNNNNNNGC 1 cut(s) 764
NciI CCSGG 2 cut(s) 120, 1085
NcoI CCATGG 1 cut(s) 1041
NdeII GATC 6 cut(s) 58, 160, 234, 376, 687, 856
NlaIII CATG 2 cut(s) 451, 1045
NlaIV GGNNCC 4 cut(s) 208, 247, 1089, 1098
NmuCI GTSAC 2 cut(s) 137, 284
NruI TCGCGA 1 cut(s) 221
NspV TTCGAA 1 cut(s) 1127
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PfeI GAWTC 4 cut(s) 95, 104, 307, 604
PflFI GACNNNGTC 1 cut(s) 793
PkrI GCNGC 5 cut(s) 269, 301, 373, 1075, 1078
PmeI GTTTAAAC 1 cut(s) 981
Psp1406I AACGTT 1 cut(s) 362
Psp6I CCWGG 2 cut(s) 588, 1101
PspGI CCWGG 2 cut(s) 588, 1101
PspN4I GGNNCC 4 cut(s) 208, 247, 1089, 1098
PspPI GGNCC 1 cut(s) 1087
PstI CTGCAG 1 cut(s) 1078
PsuI RGATCY 2 cut(s) 58, 160
PsyI GACNNNGTC 1 cut(s) 793
PvuII CAGCTG 1 cut(s) 374
RruI TCGCGA 1 cut(s) 221
RsaI GTAC 1 cut(s) 585
RsaNI GTAC 1 cut(s) 584
RseI CAYNNNNRTG 2 cut(s) 315, 384
SaqAI TTAA 5 cut(s) 177, 393, 836, 899, 980
SatI GCNGC 5 cut(s) 268, 300, 372, 1074, 1077
Sau3AI GATC 6 cut(s) 58, 160, 234, 376, 687, 856
Sau96I GGNCC 1 cut(s) 1087
ScrFI CCNGG 4 cut(s) 120, 590, 1085, 1103
SduI GDGCHC 1 cut(s) 1101
SfcI CTRYAG 3 cut(s) 171, 805, 1074
SfuI TTCGAA 1 cut(s) 1127
SinI GGWCC 1 cut(s) 1087
SmiMI CAYNNNNRTG 2 cut(s) 315, 384
SmlI CTYRAG 2 cut(s) 40, 744
SmoI CTYRAG 2 cut(s) 40, 744
Sse9I AATT 4 cut(s) 179, 443, 549, 969
SsiI CCGC 2 cut(s) 33, 74
SspI AATATT 1 cut(s) 410
SspMI CTAG 3 cut(s) 434, 519, 915
StyD4I CCNGG 4 cut(s) 118, 588, 1083, 1101
StyI CCWWGG 2 cut(s) 241, 1041
TaaI ACNGT 1 cut(s) 346
TaiI ACGT 1 cut(s) 365
TaqI TCGA 5 cut(s) 184, 624, 788, 1121, 1127
TasI AATT 4 cut(s) 179, 443, 549, 969
TatI WGTACW 1 cut(s) 583
TfiI GAWTC 4 cut(s) 95, 104, 307, 604
Tru1I TTAA 5 cut(s) 177, 393, 836, 899, 980
Tru9I TTAA 5 cut(s) 177, 393, 836, 899, 980
TscAI CASTG 1 cut(s) 895
TseFI GTSAC 2 cut(s) 137, 284
TseI GCWGC 5 cut(s) 267, 299, 371, 1073, 1076
Tsp45I GTSAC 2 cut(s) 137, 284
TspDTI ATGAA 3 cut(s) 96, 299, 436
TspRI CASTG 1 cut(s) 895
Tth111I GACNNNGTC 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 1087
XapI RAATTY 2 cut(s) 179, 443
XcmI CCANNNNNNNNNTGG 1 cut(s) 1048
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 3 cut(s) 434, 519, 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.