AT5G60090
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0026978
Physical Location & Seq
Reverse (-)
24195939 .. 24197865
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60090.1

Sequence Viewer

Length: 1197 bp
ATGAAACGTTTCTTATGCTGTCTCAAAGGAGGAAGCTCAAGTAACGCAGCGCAAGGTAACGACGAGAACGACGAAGTAGATGTCGAGCGCCTATTTGGGAATAACGAGCGCCTCAATCATCTTTGGAGAAATCATCTCCGACTCCCTGACGATCGTGAAGTTGCAGATCCGACTGCTGCTCTGTTACCAGTATCTCCACCTCTACCTGATAATTGTGGAGAATCATTTCTATGGCGTGAGCTCGTAAACGGAACCATAAACTTCCGGGACGAATTTTATCTCGGCAAAGGCAACTTCGGCGAAGTCCATCGCTGCTATTTCTCAAGACTTAATGTGGATGGAGCGGTAAAGGTTCAAAATCCACAGAATCCAACTGGTCATGTTGAGTTCTTGGCAGAGGTTACAACGTTACGTGCAACTAATCATCCAAATGTGATTAGACTTCTTGGCAACTGTTACGGCCAAAGGAACCGTGCTATAATTTACGAATTCATGGCCAACGGAGATCTTGAGCGTCACATCTTTGCACACAAAACGATGGTGGAGGGTCCGCGACCTAAGGGTCTAACACTACCAATTAGGGTTTTGGATTGGTACACGAGGATGAAAATTGCTGTGGGTGTAGCCAAGGGCCTGGTTTATCTACACGAGGAGCTGAAAGTGATTAACAGAGATGTCAAGGCCGGAAACATATTACTTGATGCAGATTTTGTTCCGAAGCTGACAGATTTTGGATTGGCGACTAAAATCGTTGAAGATGAAAACGGTGTTGAGCAGCAGCAACGGATCATTCCGATGAAGGCATCTATGGGATACATTGCACCGGAAGGAGAGATTTCCTGGTTGGTTTCGACTAAGACTGATGTCTATAGCTATGGAGCTTTTCTGTTAGTGCTTTTCACCGGAAGGAAACCTTTTTATTCGGATAATCCTGCTGGGAAAAAGAATCTCACTGATTGGTTTTTGAGAGTATGGGATAGGATCGAGGATGCGCCTGTGAAAGCTGATGTTGCGCTCGGTAATAGGTACTCGGTTGAAGGTTTAAAGAAGATATTTGATACTGCGAGGATGTGTATAAAGGCAGAGAGACTTGAACGGCCTACCATGAGTGAAGTGGAAGCGATGGTTCTCGAAGCTGCAGCTTTCCCGGTCCAAGTGCCGCGCCAGGTGACAAGGAGGCGTTCGGCTTCAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr

Protein Analysis

398

Amino Acids

44.98

Weight (kDa)

7.63

Isoelectric Point (pI)

28.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 93 - 372 3e-35 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 94 - 373 1.4e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0026978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60080 AT5G60090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 561
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 2 cut(s) 553, 1162
AciI CCGC 3 cut(s) 344, 551, 1160
AclI AACGTT 2 cut(s) 7, 407
AclWI GGATC 3 cut(s) 161, 794, 989
AcoI YGGCCR 2 cut(s) 460, 495
AcsI RAATTY 2 cut(s) 272, 488
AfaI GTAC 2 cut(s) 596, 1028
AgsI TTSAA 5 cut(s) 356, 755, 1037, 1094, 1191
AjnI CCWGG 3 cut(s) 633, 839, 1164
AluBI AGCT 9 cut(s) 36, 241, 655, 721, 873, 881, 1004, 1136, 1142
AluI AGCT 9 cut(s) 36, 241, 655, 721, 873, 881, 1004, 1136, 1142
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 2 cut(s) 26, 1081
AlwI GGATC 3 cut(s) 161, 794, 989
AoxI GGCC 5 cut(s) 460, 495, 631, 681, 1097
ApeKI GCWGC 7 cut(s) 47, 176, 312, 775, 778, 1136, 1139
ApoI RAATTY 2 cut(s) 272, 488
ArsI GACNNNNNNTTYG 2 cut(s) 66, 98
Asp700I GAANNNNTTC 2 cut(s) 8, 225
AspLEI GCGC 6 cut(s) 52, 90, 111, 994, 1015, 1164
AspS9I GGNCC 3 cut(s) 548, 631, 1150
AsuC2I CCSGG 2 cut(s) 266, 1148
AsuHPI GGTGA 2 cut(s) 892, 1180
AvaII GGWCC 2 cut(s) 548, 1150
AxyI CCTNAGG 1 cut(s) 558
BalI TGGCCA 1 cut(s) 497
BanII GRGCYC 1 cut(s) 243
BauI CACGAG 2 cut(s) 598, 647
Bbv12I GWGCWC 1 cut(s) 243
BbvI GCAGC 7 cut(s) 59, 163, 299, 787, 790, 1123, 1151
BccI CCATC 4 cut(s) 315, 332, 532, 1117
BceAI ACGGC 2 cut(s) 475, 1112
BciT130I CCWGG 3 cut(s) 635, 841, 1166
BciVI GTATCC 1 cut(s) 806
BcnI CCSGG 2 cut(s) 266, 1148
BcoDI GTCTC 2 cut(s) 26, 1081
BfmI CTRYAG 2 cut(s) 868, 1137
BfoI RGCGCY 2 cut(s) 91, 112
BfuI GTATCC 1 cut(s) 806
BglII AGATCT 1 cut(s) 505
BisI GCNGC 8 cut(s) 48, 177, 313, 776, 779, 1137, 1140, 1160
BlsI GCNGC 8 cut(s) 49, 178, 314, 777, 780, 1138, 1141, 1161
Bme1390I CCNGG 5 cut(s) 266, 635, 841, 1148, 1166
Bme18I GGWCC 2 cut(s) 548, 1150
BmgT120I GGNCC 3 cut(s) 548, 631, 1150
BmiI GGNNCC 3 cut(s) 253, 470, 549
BmrFI CCNGG 5 cut(s) 266, 635, 841, 1148, 1166
BmsI GCATC 3 cut(s) 691, 812, 979
BoxI GACNNNNGTC 1 cut(s) 863
BpuEI CTTGAG 3 cut(s) 22, 307, 530
BpuMI CCSGG 2 cut(s) 266, 1148
BsaAI YACGTR 1 cut(s) 413
BsaJI CCNNGG 1 cut(s) 627
BsaWI WCCGGW 2 cut(s) 823, 902
Bse1I ACTGG 2 cut(s) 188, 379
Bse21I CCTNAGG 1 cut(s) 558
Bse3DI GCAATG 1 cut(s) 816
BseBI CCWGG 3 cut(s) 635, 841, 1166
BseDI CCNNGG 1 cut(s) 627
BseGI GGATG 5 cut(s) 343, 424, 609, 994, 1074
BseMI GCAATG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 188, 379
BseRI GAGGAG 1 cut(s) 665
BseXI GCAGC 7 cut(s) 59, 163, 299, 787, 790, 1123, 1151
BseYI CCCAGC 1 cut(s) 935
Bsh1236I CGCG 2 cut(s) 553, 1162
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 5 cut(s) 462, 497, 633, 683, 1099
BsiEI CGRYCG 1 cut(s) 154
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 5 cut(s) 265, 684, 824, 903, 1148
BslFI GGGAC 1 cut(s) 281
BsmAI GTCTC 2 cut(s) 26, 1081
BsmFI GGGAC 1 cut(s) 281
BsnI GGCC 5 cut(s) 462, 497, 633, 683, 1099
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 5 cut(s) 151, 166, 505, 786, 981
BspACI CCGC 3 cut(s) 344, 551, 1160
BspANI GGCC 5 cut(s) 462, 497, 633, 683, 1099
BspFNI CGCG 2 cut(s) 553, 1162
BspLI GGNNCC 3 cut(s) 253, 470, 549
BspMAI CTGCAG 1 cut(s) 1141
BspPI GGATC 3 cut(s) 161, 794, 989
BsrBI CCGCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 816
BsrI ACTGG 2 cut(s) 188, 379
BssECI CCNNGG 1 cut(s) 627
BssMI GATC 5 cut(s) 151, 166, 505, 786, 981
BssSI CACGAG 2 cut(s) 598, 647
BssT1I CCWWGG 1 cut(s) 627
Bst2BI CACGAG 2 cut(s) 598, 647
Bst2UI CCWGG 3 cut(s) 635, 841, 1166
Bst4CI ACNGT 3 cut(s) 455, 473, 767
BstBAI YACGTR 1 cut(s) 413
BstDEI CTNAG 2 cut(s) 558, 855
BstF5I GGATG 5 cut(s) 343, 424, 609, 994, 1074
BstFNI CGCG 2 cut(s) 553, 1162
BstH2I RGCGCY 2 cut(s) 91, 112
BstHHI GCGC 6 cut(s) 52, 90, 111, 994, 1015, 1164
BstKTI GATC 5 cut(s) 154, 169, 508, 789, 984
BstMAI GTCTC 2 cut(s) 26, 1081
BstMBI GATC 5 cut(s) 151, 166, 505, 786, 981
BstMCI CGRYCG 1 cut(s) 154
BstMWI GCNNNNNNNGC 2 cut(s) 297, 1010
BstNI CCWGG 3 cut(s) 635, 841, 1166
BstPAI GACNNNNGTC 1 cut(s) 863
BstSCI CCNGG 5 cut(s) 264, 633, 839, 1146, 1164
BstSFI CTRYAG 2 cut(s) 868, 1137
BstUI CGCG 2 cut(s) 553, 1162
BstV1I GCAGC 7 cut(s) 59, 163, 299, 787, 790, 1123, 1151
BstX2I RGATCY 2 cut(s) 166, 505
BstXI CCANNNNNNTGG 1 cut(s) 634
BstYI RGATCY 2 cut(s) 166, 505
Bsu36I CCTNAGG 1 cut(s) 558
BsuI GTATCC 1 cut(s) 806
BsuRI GGCC 5 cut(s) 462, 497, 633, 683, 1099
BtgZI GCGATG 2 cut(s) 293, 1136
BtsCI GGATG 5 cut(s) 343, 424, 609, 994, 1074
BtsIMutI CAGTG 1 cut(s) 951
CfoI GCGC 6 cut(s) 52, 90, 111, 994, 1015, 1164
Cfr13I GGNCC 3 cut(s) 548, 631, 1150
CseI GACGC 1 cut(s) 503
Csp6I GTAC 2 cut(s) 595, 1027
CviAII CATG 3 cut(s) 380, 493, 1105
CviQI GTAC 2 cut(s) 595, 1027
DdeI CTNAG 2 cut(s) 558, 855
DpnI GATC 5 cut(s) 153, 168, 507, 788, 983
DpnII GATC 5 cut(s) 151, 166, 505, 786, 981
DraI TTTAAA 1 cut(s) 1044
DrdI GACNNNNNNGTC 1 cut(s) 561
DseDI GACNNNNNNGTC 1 cut(s) 561
EaeI YGGCCR 2 cut(s) 460, 495
Ecl136II GAGCTC 1 cut(s) 241
Eco130I CCWWGG 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 243
Eco47I GGWCC 2 cut(s) 548, 1150
Eco53kI GAGCTC 1 cut(s) 241
Eco81I CCTNAGG 1 cut(s) 558
EcoICRI GAGCTC 1 cut(s) 241
EcoO109I RGGNCCY 1 cut(s) 631
EcoRI GAATTC 1 cut(s) 488
EcoRII CCWGG 3 cut(s) 633, 839, 1164
EcoT14I CCWWGG 1 cut(s) 627
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 1 cut(s) 627
FaeI CATG 3 cut(s) 383, 496, 1108
FalI AAGNNNNNCTT 2 cut(s) 898, 930
FaqI GGGAC 1 cut(s) 281
FatI CATG 3 cut(s) 379, 492, 1104
Fnu4HI GCNGC 8 cut(s) 48, 177, 313, 776, 779, 1137, 1140, 1160
FokI GGATG 5 cut(s) 350, 411, 616, 1001, 1081
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 8 cut(s) 48, 177, 313, 776, 779, 1137, 1140, 1160
GlaI GCGC 6 cut(s) 51, 89, 110, 993, 1014, 1163
GluI GCNGC 8 cut(s) 48, 177, 313, 776, 779, 1137, 1140, 1160
GsaI CCCAGC 1 cut(s) 939
HaeII RGCGCY 2 cut(s) 91, 112
HaeIII GGCC 5 cut(s) 462, 497, 633, 683, 1099
HapII CCGG 5 cut(s) 265, 684, 824, 903, 1148
HgaI GACGC 1 cut(s) 503
HhaI GCGC 6 cut(s) 52, 90, 111, 994, 1015, 1164
Hin1II CATG 3 cut(s) 383, 496, 1108
Hin6I GCGC 6 cut(s) 50, 88, 109, 992, 1013, 1162
HinP1I GCGC 6 cut(s) 50, 88, 109, 992, 1013, 1162
HinfI GANTC 4 cut(s) 141, 221, 367, 946
HpaII CCGG 5 cut(s) 265, 684, 824, 903, 1148
HphI GGTGA 2 cut(s) 892, 1180
Hpy166II GTNNAC 2 cut(s) 247, 597
Hpy188I TCNGA 5 cut(s) 140, 171, 717, 795, 925
Hpy188III TCNNGA 4 cut(s) 155, 324, 509, 1130
Hpy8I GTNNAC 2 cut(s) 247, 597
Hpy99I CGWCG 2 cut(s) 65, 74
HpyAV CCTTC 4 cut(s) 793, 821, 900, 1031
HpyCH4III ACNGT 3 cut(s) 455, 473, 767
HpyCH4IV ACGT 3 cut(s) 7, 407, 412
HpyCH4V TGCA 6 cut(s) 164, 416, 527, 704, 821, 1139
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 1010
HpyF3I CTNAG 2 cut(s) 558, 855
HpySE526I ACGT 3 cut(s) 7, 407, 412
Hsp92II CATG 3 cut(s) 383, 496, 1108
HspAI GCGC 6 cut(s) 50, 88, 109, 992, 1013, 1162
Kzo9I GATC 5 cut(s) 151, 166, 505, 786, 981
LmnI GCTCC 3 cut(s) 341, 652, 878
Lsp1109I GCAGC 7 cut(s) 59, 163, 299, 787, 790, 1123, 1151
LweI GCATC 3 cut(s) 691, 812, 979
MaeII ACGT 3 cut(s) 7, 407, 412
MaeIII GTNAC 8 cut(s) 41, 56, 183, 400, 408, 455, 515, 1168
MalI GATC 5 cut(s) 153, 168, 507, 788, 983
MbiI CCGCTC 1 cut(s) 344
MboI GATC 5 cut(s) 151, 166, 505, 786, 981
MboII GAAGA 2 cut(s) 767, 1060
MflI RGATCY 2 cut(s) 166, 505
MhlI GDGCHC 1 cut(s) 243
MlsI TGGCCA 1 cut(s) 497
MluCI AATT 6 cut(s) 211, 272, 480, 488, 576, 609
MluNI TGGCCA 1 cut(s) 497
MlyI GAGTC 1 cut(s) 135
MmeI TCCRAC 3 cut(s) 163, 194, 395
Mox20I TGGCCA 1 cut(s) 497
MroXI GAANNNNTTC 2 cut(s) 8, 225
MscI TGGCCA 1 cut(s) 497
MseI TTAA 3 cut(s) 330, 666, 1043
MslI CAYNNNNRTG 4 cut(s) 229, 429, 602, 794
Msp20I TGGCCA 1 cut(s) 497
MspI CCGG 5 cut(s) 265, 684, 824, 903, 1148
MspR9I CCNGG 5 cut(s) 266, 635, 841, 1148, 1166
MvaI CCWGG 3 cut(s) 635, 841, 1166
MvnI CGCG 2 cut(s) 553, 1162
MwoI GCNNNNNNNGC 2 cut(s) 297, 1010
NciI CCSGG 2 cut(s) 266, 1148
NdeII GATC 5 cut(s) 151, 166, 505, 786, 981
NlaIII CATG 3 cut(s) 383, 496, 1108
NlaIV GGNNCC 3 cut(s) 253, 470, 549
NmeAIII GCCGAG 1 cut(s) 261
NmuCI GTSAC 2 cut(s) 515, 1168
PcsI WCGNNNNNNNCGW 2 cut(s) 66, 69
PdmI GAANNNNTTC 2 cut(s) 8, 225
PfeI GAWTC 3 cut(s) 221, 367, 946
PfoI TCCNGGA 1 cut(s) 264
PkrI GCNGC 8 cut(s) 49, 178, 314, 777, 780, 1138, 1141, 1161
Ple19I CGATCG 1 cut(s) 154
PleI GAGTC 1 cut(s) 135
PpsI GAGTC 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 413
PshAI GACNNNNGTC 1 cut(s) 863
Psp124BI GAGCTC 1 cut(s) 243
Psp1406I AACGTT 2 cut(s) 7, 407
Psp6I CCWGG 3 cut(s) 633, 839, 1164
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 3 cut(s) 633, 839, 1164
PspN4I GGNNCC 3 cut(s) 253, 470, 549
PspPI GGNCC 3 cut(s) 548, 631, 1150
PstI CTGCAG 1 cut(s) 1141
PsuI RGATCY 2 cut(s) 166, 505
PvuI CGATCG 1 cut(s) 154
RsaI GTAC 2 cut(s) 596, 1028
RsaNI GTAC 2 cut(s) 595, 1027
RseI CAYNNNNRTG 4 cut(s) 229, 429, 602, 794
SacI GAGCTC 1 cut(s) 243
SaqAI TTAA 3 cut(s) 330, 666, 1043
SatI GCNGC 8 cut(s) 48, 177, 313, 776, 779, 1137, 1140, 1160
Sau3AI GATC 5 cut(s) 151, 166, 505, 786, 981
Sau96I GGNCC 3 cut(s) 548, 631, 1150
SchI GAGTC 1 cut(s) 135
ScrFI CCNGG 5 cut(s) 266, 635, 841, 1148, 1166
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 3 cut(s) 691, 812, 979
SfcI CTRYAG 2 cut(s) 868, 1137
SinI GGWCC 2 cut(s) 548, 1150
SmiMI CAYNNNNRTG 4 cut(s) 229, 429, 602, 794
SmlI CTYRAG 3 cut(s) 37, 322, 509
SmoI CTYRAG 3 cut(s) 37, 322, 509
Sse9I AATT 6 cut(s) 211, 272, 480, 488, 576, 609
SsiI CCGC 3 cut(s) 344, 551, 1160
SstI GAGCTC 1 cut(s) 243
StyD4I CCNGG 5 cut(s) 264, 633, 839, 1146, 1164
StyI CCWWGG 1 cut(s) 627
TaaI ACNGT 3 cut(s) 455, 473, 767
TaiI ACGT 3 cut(s) 10, 410, 415
TaqI TCGA 4 cut(s) 84, 851, 984, 1131
TasI AATT 6 cut(s) 211, 272, 480, 488, 576, 609
TauI GCSGC 1 cut(s) 1162
TfiI GAWTC 3 cut(s) 221, 367, 946
Tru1I TTAA 3 cut(s) 330, 666, 1043
Tru9I TTAA 3 cut(s) 330, 666, 1043
TscAI CASTG 1 cut(s) 958
TseFI GTSAC 2 cut(s) 515, 1168
TseI GCWGC 7 cut(s) 47, 176, 312, 775, 778, 1136, 1139
Tsp45I GTSAC 2 cut(s) 515, 1168
TspDTI ATGAA 5 cut(s) 17, 481, 620, 774, 812
TspGWI ACGGA 3 cut(s) 264, 516, 799
TspRI CASTG 1 cut(s) 958
VpaK11BI GGWCC 2 cut(s) 548, 1150
XapI RAATTY 2 cut(s) 272, 488
XcmI CCANNNNNNNNNTGG 1 cut(s) 1111
XmnI GAANNNNTTC 2 cut(s) 8, 225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.