AT5G66290

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0026703
Physical Location & Seq
Reverse (-)
26477296 .. 26479590
2295 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66290.3

Sequence Viewer

Length: 609 bp
ATGGGAGCATCAGATTCAACTCTGCTAGGCTCTCAGGAAAATCGAGGTGATGTGATTACGACTATATCTCATCGATCAGAAATAGTCGATCCCATTCTCGAGAATCTTAAAGCTCTCACAGTAAGTAGGCCAATATTGAAGTCTCCTCCCACGGAAAGTAGCTTAACAGATATCTTAGTGAGAAAAGCTCTATCCAGTTCCTCTTCCAATACGGTTGATCCTCAAATACTTGTAGAGCTCTTCTCCATTTACCGAGAATGGCAAGAGAACAAAGCTCAAGAAATTACCAAGAGACAGGAAGATATAGAAAACAAGATAGAAGTCGCAGATGCTTTGGCGACTAAACTTTTACAGAGGTTTAACCACTCTGTTTCTGCAATGAGGACAACTGCCCAGCATTTATCTCAAGTTCATGGTTTGCAGGTGGAGCTCGGGGAGCTTAAAGGAAGATTGACAGAGGTGATCAGCAACTGCGATACATTATGTAAGAGAATTAATTCTGAGGGACCTGAATCTCTGAGGTCCACAGTGGCGCCTTTTTCTCTTGCATCTCCCGATTCGGTCAGTATAAATACTACTACAACTCTGTCTTCTAAACCCGAGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

202

Amino Acids

22.1

Weight (kDa)

5.44

Isoelectric Point (pI)

56.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0026703)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66290
rosa_wichuraiana Rw0G001770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 412
AccB1I GGYRCC 1 cut(s) 532
AclWI GGATC 2 cut(s) 83, 212
AcyI GRCGYC 1 cut(s) 533
AgsI TTSAA 2 cut(s) 18, 139
AluBI AGCT 7 cut(s) 113, 162, 188, 238, 275, 430, 439
AluI AGCT 7 cut(s) 113, 162, 188, 238, 275, 430, 439
Alw21I GWGCWC 2 cut(s) 240, 432
Alw26I GTCTC 2 cut(s) 147, 286
AlwI GGATC 2 cut(s) 83, 212
AlwNI CAGNNNCTG 1 cut(s) 471
Ama87I CYCGRG 3 cut(s) 98, 431, 599
AoxI GGCC 1 cut(s) 128
AseI ATTAAT 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 496
AspLEI GCGC 1 cut(s) 535
AspS9I GGNCC 2 cut(s) 506, 522
AsuHPI GGTGA 2 cut(s) 59, 472
AvaI CYCGRG 3 cut(s) 98, 431, 599
AvaII GGWCC 2 cut(s) 506, 522
BanI GGYRCC 1 cut(s) 532
BanII GRGCYC 2 cut(s) 240, 432
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 2 cut(s) 240, 432
BclI TGATCA 1 cut(s) 462
BcoDI GTCTC 2 cut(s) 147, 286
BfaI CTAG 1 cut(s) 26
BfoI RGCGCY 1 cut(s) 536
BfuAI ACCTGC 1 cut(s) 412
Bme18I GGWCC 2 cut(s) 506, 522
BmeT110I CYCGRG 3 cut(s) 98, 431, 599
BmgT120I GGNCC 2 cut(s) 506, 522
BmiI GGNNCC 2 cut(s) 507, 534
BmsI GCATC 3 cut(s) 17, 319, 557
BpiI GAAGAC 1 cut(s) 582
BplI GAGNNNNNCTC 4 cut(s) 172, 204, 227, 259
BpuEI CTTGAG 2 cut(s) 261, 390
Bsa29I ATCGAT 1 cut(s) 73
BsaHI GRCGYC 1 cut(s) 533
BsaJI CCNNGG 2 cut(s) 150, 600
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 1 cut(s) 384
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 150, 600
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 3 cut(s) 47, 492, 509
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 135
BseYI CCCAGC 1 cut(s) 393
BshFI GGCC 1 cut(s) 130
BshNI GGYRCC 1 cut(s) 532
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 240, 432
BsiHKCI CYCGRG 3 cut(s) 98, 431, 599
BslFI GGGAC 1 cut(s) 519
BsmAI GTCTC 2 cut(s) 147, 286
BsmFI GGGAC 1 cut(s) 519
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 3 cut(s) 98, 431, 599
Bsp1286I GDGCHC 2 cut(s) 240, 432
Bsp143I GATC 4 cut(s) 74, 88, 217, 462
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 3 cut(s) 46, 493, 510
BspDI ATCGAT 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 507, 534
BspMI ACCTGC 1 cut(s) 412
BspPI GGATC 2 cut(s) 83, 212
BspQI GCTCTTC 1 cut(s) 245
BspT107I GGYRCC 1 cut(s) 532
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 150, 600
BssMI GATC 4 cut(s) 74, 88, 217, 462
BssNI GRCGYC 1 cut(s) 533
Bst4CI ACNGT 3 cut(s) 121, 214, 529
Bst6I CTCTTC 2 cut(s) 208, 245
BstACI GRCGYC 1 cut(s) 533
BstDEI CTNAG 4 cut(s) 33, 175, 501, 518
BstDSI CCRYGG 1 cut(s) 150
BstH2I RGCGCY 1 cut(s) 536
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 4 cut(s) 77, 91, 220, 465
BstMAI GTCTC 2 cut(s) 147, 286
BstMBI GATC 4 cut(s) 74, 88, 217, 462
BstMWI GCNNNNNNNGC 2 cut(s) 427, 436
BstV2I GAAGAC 1 cut(s) 582
Bsu15I ATCGAT 1 cut(s) 73
BsuRI GGCC 1 cut(s) 130
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 534
BveI ACCTGC 1 cut(s) 412
CaiI CAGNNNCTG 1 cut(s) 471
CfoI GCGC 1 cut(s) 535
Cfr13I GGNCC 2 cut(s) 506, 522
ClaI ATCGAT 1 cut(s) 73
CviAII CATG 1 cut(s) 413
CviJI RGCY 9 cut(s) 30, 113, 130, 162, 188, 238, 275, 430, 439
CviKI_1 RGCY 9 cut(s) 30, 113, 130, 162, 188, 238, 275, 430, 439
DdeI CTNAG 4 cut(s) 33, 175, 501, 518
DinI GGCGCC 1 cut(s) 534
DpnI GATC 4 cut(s) 76, 90, 219, 464
DpnII GATC 4 cut(s) 74, 88, 217, 462
Eam1104I CTCTTC 2 cut(s) 208, 245
EarI CTCTTC 2 cut(s) 208, 245
Ecl136II GAGCTC 2 cut(s) 238, 430
Eco24I GRGCYC 2 cut(s) 240, 432
Eco32I GATATC 1 cut(s) 172
Eco47I GGWCC 2 cut(s) 506, 522
Eco53kI GAGCTC 2 cut(s) 238, 430
Eco88I CYCGRG 3 cut(s) 98, 431, 599
EcoICRI GAGCTC 2 cut(s) 238, 430
EcoO109I RGGNCCY 1 cut(s) 506
EcoRV GATATC 1 cut(s) 172
EcoT38I GRGCYC 2 cut(s) 240, 432
EgeI GGCGCC 1 cut(s) 534
EheI GGCGCC 1 cut(s) 534
FaeI CATG 1 cut(s) 416
FaiI YATR 6 cut(s) 65, 305, 414, 484, 569, 607
FaqI GGGAC 1 cut(s) 519
FatI CATG 1 cut(s) 412
FbaI TGATCA 1 cut(s) 462
FriOI GRGCYC 2 cut(s) 240, 432
FspBI CTAG 1 cut(s) 26
GlaI GCGC 1 cut(s) 534
GsaI CCCAGC 1 cut(s) 397
HaeII RGCGCY 1 cut(s) 536
HaeIII GGCC 1 cut(s) 130
HhaI GCGC 1 cut(s) 535
Hin1I GRCGYC 1 cut(s) 533
Hin1II CATG 1 cut(s) 416
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HinfI GANTC 4 cut(s) 14, 103, 512, 557
HphI GGTGA 2 cut(s) 59, 472
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 4 cut(s) 13, 79, 502, 519
Hpy188III TCNNGA 5 cut(s) 35, 98, 100, 278, 554
Hpy8I GTNNAC 1 cut(s) 525
HpyCH4III ACNGT 3 cut(s) 121, 214, 529
HpyCH4V TGCA 3 cut(s) 377, 421, 548
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 436
HpyF3I CTNAG 4 cut(s) 33, 175, 501, 518
Hsp92I GRCGYC 1 cut(s) 533
Hsp92II CATG 1 cut(s) 416
HspAI GCGC 1 cut(s) 533
KasI GGCGCC 1 cut(s) 532
Ksp22I TGATCA 1 cut(s) 462
Kzo9I GATC 4 cut(s) 74, 88, 217, 462
LguI GCTCTTC 1 cut(s) 245
LmnI GCTCC 3 cut(s) 5, 427, 436
LpnPI CCDG 6 cut(s) 20, 208, 281, 407, 407, 522
LweI GCATC 3 cut(s) 17, 319, 557
MaeI CTAG 1 cut(s) 26
MalI GATC 4 cut(s) 76, 90, 219, 464
MboI GATC 4 cut(s) 74, 88, 217, 462
MboII GAAGA 5 cut(s) 195, 232, 311, 459, 582
MhlI GDGCHC 2 cut(s) 240, 432
MluCI AATT 3 cut(s) 282, 492, 496
Mly113I GGCGCC 1 cut(s) 533
MroXI GAANNNNTTC 1 cut(s) 496
MseI TTAA 5 cut(s) 108, 164, 360, 441, 495
MwoI GCNNNNNNNGC 2 cut(s) 427, 436
NarI GGCGCC 1 cut(s) 533
NdeII GATC 4 cut(s) 74, 88, 217, 462
NlaIII CATG 1 cut(s) 416
NlaIV GGNNCC 2 cut(s) 507, 534
PaeR7I CTCGAG 1 cut(s) 98
PaqCI CACCTGC 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 245
PdmI GAANNNNTTC 1 cut(s) 496
PfeI GAWTC 4 cut(s) 14, 103, 512, 557
PluTI GGCGCC 1 cut(s) 536
PpuMI RGGWCCY 1 cut(s) 506
PshBI ATTAAT 1 cut(s) 495
Psp124BI GAGCTC 2 cut(s) 240, 432
Psp5II RGGWCCY 1 cut(s) 506
PspFI CCCAGC 1 cut(s) 393
PspN4I GGNNCC 2 cut(s) 507, 534
PspPI GGNCC 2 cut(s) 506, 522
PspPPI RGGWCCY 1 cut(s) 506
PstNI CAGNNNCTG 1 cut(s) 471
SacI GAGCTC 2 cut(s) 240, 432
SapI GCTCTTC 1 cut(s) 245
SaqAI TTAA 5 cut(s) 108, 164, 360, 441, 495
Sau3AI GATC 4 cut(s) 74, 88, 217, 462
Sau96I GGNCC 2 cut(s) 506, 522
SduI GDGCHC 2 cut(s) 240, 432
SfaNI GCATC 3 cut(s) 17, 319, 557
SfoI GGCGCC 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 2 cut(s) 506, 522
SlaI CTCGAG 1 cut(s) 98
SmlI CTYRAG 3 cut(s) 98, 276, 405
SmoI CTYRAG 3 cut(s) 98, 276, 405
Sse9I AATT 3 cut(s) 282, 492, 496
SspDI GGCGCC 1 cut(s) 532
SspI AATATT 1 cut(s) 135
SspMI CTAG 1 cut(s) 26
SstI GAGCTC 2 cut(s) 240, 432
TaaI ACNGT 3 cut(s) 121, 214, 529
TaqI TCGA 4 cut(s) 43, 73, 87, 99
TaqII GACCGA 1 cut(s) 550
TasI AATT 3 cut(s) 282, 492, 496
TfiI GAWTC 4 cut(s) 14, 103, 512, 557
Tru1I TTAA 5 cut(s) 108, 164, 360, 441, 495
Tru9I TTAA 5 cut(s) 108, 164, 360, 441, 495
TscAI CASTG 1 cut(s) 534
TspDTI ATGAA 1 cut(s) 401
TspGWI ACGGA 1 cut(s) 167
TspRI CASTG 1 cut(s) 534
VpaK11BI GGWCC 2 cut(s) 506, 522
VspI ATTAAT 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 98
XmnI GAANNNNTTC 1 cut(s) 496
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.