ATCG00770

One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0020235
Physical Location & Seq
Reverse (-)
80068 .. 80472
405 bp
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UTR
Exon/CDS
Intron
ATCG00770.1

Sequence Viewer

Length: 405 bp
ATGGGGAAAGACACCATTGCTGATATAATAACCTCTATACGAAATGCTGACATGAATAGAAAAGGAACGGTTCGGATAGGATCGACTAACATCACGGAAAGTATTGTTAAAATCCTTTTACGAGAGGGTTTTATCGAAAACGTAAGGAAACATCGCGAAAACAACCAATATTTTTTGATTTTAACCCTAAGACATAGACGAAATAAGAAAGAATCCTACAAAACGATTTTAAATTTAAAGAGAATAAGTCGACCGGGTCTACGAATCTATTCTAACTCTCAACGAATTCCACGAATTTTAGGCGGAATAGGAATTGTAATCCTTTCGACTTCTCAAGGTATAATGACAGACCGAGAAGCTCGACTAAAAAGAATCGGCGGAGAAATTTTGTGTTATATATGGTAA

Protein Analysis

134

Amino Acids

15.48

Weight (kDa)

10.94

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S8 PF00410 4 - 134 4.8e-42 Ribosomal protein S8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020235)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00770
rosa_chinensis RchiOBHm_CPg0501871
rosa_roxburghii Rroxscaffold_2G00119000
rosa_samantha Rh1CG032600
rosa_wichuraiana Rw7G013540 Rw7G019280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 250, 259
AccII CGCG 1 cut(s) 156
AciI CCGC 2 cut(s) 303, 378
AclWI GGATC 1 cut(s) 88
AcsI RAATTY 4 cut(s) 232, 285, 294, 384
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
AlwI GGATC 1 cut(s) 88
ApoI RAATTY 4 cut(s) 232, 285, 294, 384
Asp700I GAANNNNTTC 1 cut(s) 268
AsuC2I CCSGG 1 cut(s) 255
BcnI CCSGG 1 cut(s) 255
Bme1390I CCNGG 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 15
BseMI GCAATG 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 254
BsiEI CGRYCG 1 cut(s) 254
BsiSI CCGG 1 cut(s) 254
Bsp143I GATC 1 cut(s) 80
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 2 cut(s) 303, 378
BspFNI CGCG 1 cut(s) 156
BspPI GGATC 1 cut(s) 88
BsrDI GCAATG 1 cut(s) 15
BssMI GATC 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 70
BstDEI CTNAG 1 cut(s) 188
BstFNI CGCG 1 cut(s) 156
BstKTI GATC 1 cut(s) 83
BstMBI GATC 1 cut(s) 80
BstMCI CGRYCG 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 253
BstUI CGCG 1 cut(s) 156
BtgZI GCGATG 1 cut(s) 137
BtuMI TCGCGA 1 cut(s) 156
CviAII CATG 1 cut(s) 52
CviJI RGCY 1 cut(s) 359
CviKI_1 RGCY 1 cut(s) 359
DdeI CTNAG 1 cut(s) 188
DpnI GATC 1 cut(s) 82
DpnII GATC 1 cut(s) 80
DraI TTTAAA 2 cut(s) 231, 237
EciI GGCGGA 2 cut(s) 318, 393
EcoRI GAATTC 1 cut(s) 285
FaeI CATG 1 cut(s) 55
FaiI YATR 8 cut(s) 26, 38, 53, 195, 341, 396, 398, 400
FatI CATG 1 cut(s) 51
FblI GTMKAC 2 cut(s) 250, 259
HapII CCGG 1 cut(s) 254
Hin1II CATG 1 cut(s) 55
HincII GTYRAC 1 cut(s) 251
HindII GTYRAC 1 cut(s) 251
HinfI GANTC 3 cut(s) 212, 264, 372
HpaII CCGG 1 cut(s) 254
Hpy166II GTNNAC 2 cut(s) 251, 260
Hpy188I TCNGA 1 cut(s) 75
Hpy188III TCNNGA 1 cut(s) 155
Hpy8I GTNNAC 2 cut(s) 251, 260
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 141
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 141
Hsp92II CATG 1 cut(s) 55
Kzo9I GATC 1 cut(s) 80
LpnPI CCDG 1 cut(s) 267
MaeII ACGT 1 cut(s) 141
MalI GATC 1 cut(s) 82
MboI GATC 1 cut(s) 80
MluCI AATT 5 cut(s) 232, 285, 294, 312, 384
MnlI CCTC 2 cut(s) 43, 118
MroXI GAANNNNTTC 1 cut(s) 268
MseI TTAA 4 cut(s) 108, 182, 230, 236
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 156
NciI CCSGG 1 cut(s) 255
NdeII GATC 1 cut(s) 80
NlaIII CATG 1 cut(s) 55
NruI TCGCGA 1 cut(s) 156
PcsI WCGNNNNNNNCGW 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 268
PfeI GAWTC 3 cut(s) 212, 264, 372
PflFI GACNNNGTC 1 cut(s) 255
PsyI GACNNNGTC 1 cut(s) 255
RruI TCGCGA 1 cut(s) 156
SalI GTCGAC 1 cut(s) 249
SaqAI TTAA 4 cut(s) 108, 182, 230, 236
Sau3AI GATC 1 cut(s) 80
ScrFI CCNGG 1 cut(s) 255
SetI ASST 4 cut(s) 35, 144, 340, 361
SmlI CTYRAG 1 cut(s) 333
SmoI CTYRAG 1 cut(s) 333
Sse9I AATT 5 cut(s) 232, 285, 294, 312, 384
SsiI CCGC 2 cut(s) 303, 378
SspI AATATT 1 cut(s) 170
StyD4I CCNGG 1 cut(s) 253
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 144
TaqI TCGA 5 cut(s) 83, 135, 250, 326, 361
TaqII GACCGA 1 cut(s) 366
TasI AATT 5 cut(s) 232, 285, 294, 312, 384
TfiI GAWTC 3 cut(s) 212, 264, 372
Tru1I TTAA 4 cut(s) 108, 182, 230, 236
Tru9I TTAA 4 cut(s) 108, 182, 230, 236
TspDTI ATGAA 1 cut(s) 68
TspGWI ACGGA 1 cut(s) 110
Tth111I GACNNNGTC 1 cut(s) 255
XapI RAATTY 4 cut(s) 232, 285, 294, 384
XmiI GTMKAC 2 cut(s) 250, 259
XmnI GAANNNNTTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.