FvH4_2g05762

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Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0019198
Physical Location & Seq
Forward (+)
4723955 .. 4724592
638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g05762.t1

Sequence Viewer

Length: 441 bp
ATGGCATCTTCACTAACCCTAAGGCGCCTCCTCATGCCAAAATCCCTTGCTCCAATATCATCATCGACTCGCTCTATCACCACCACCCTGTCGGCTGCCGCCAAGCACTTCATCTCCACTGCTCCTGCTGATTACGGCGACGACGGCGAGGGGGAGTGGTACACGCAGGAGACGGACAAAGCTTATTATGTGAGGTTGAATTTGCCGGAATATGAGAAAAAAGACGTGAAGGTGGACGTGGACCGAAAAGAGAACATTCTTACCGTCACTGGCCGGGCCGGGCATGGCAGGCATTGGACCTCTTATGACCTGCCGGAGGAATGCATCATCGATTACACCAAGGCAGAGATGAACGACGGCGTGCTGAAGGTTGTTGTGCCTAAGGTCCTCGATGAAGACGAGGATCAGAAGAGTGAAAGCTTTAGTGTCCACATAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

147

Amino Acids

16.43

Weight (kDa)

5.02

Isoelectric Point (pI)

47.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 56 - 136 6.9e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019198)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05762
pyrus_communis pycom01g19310
rosa_laevigata RLG00000014742
rosa_multiflora Rmu_sc0007004.1_g000009
rosa_samantha Rh6AG083300 Rh6BG077900 Rh6CG072500 Rh6DG069700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 318
AccB1I GGYRCC 1 cut(s) 24
AciI CCGC 1 cut(s) 99
AclWI GGATC 1 cut(s) 411
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 25
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 1 cut(s) 199
AjiI CACGTC 2 cut(s) 226, 238
AluBI AGCT 2 cut(s) 182, 420
AluI AGCT 2 cut(s) 182, 420
Alw26I GTCTC 1 cut(s) 164
AlwI GGATC 1 cut(s) 411
AoxI GGCC 2 cut(s) 271, 276
ApeKI GCWGC 1 cut(s) 95
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 27
AspS9I GGNCC 4 cut(s) 241, 276, 297, 385
AsuC2I CCSGG 2 cut(s) 275, 280
AsuHPI GGTGA 1 cut(s) 70
AvaII GGWCC 3 cut(s) 241, 297, 385
AxyI CCTNAGG 2 cut(s) 20, 381
BanI GGYRCC 1 cut(s) 24
BbsI GAAGAC 1 cut(s) 402
BbvI GCAGC 1 cut(s) 82
BceAI ACGGC 3 cut(s) 151, 160, 373
BcnI CCSGG 2 cut(s) 275, 280
BcoDI GTCTC 1 cut(s) 164
BfoI RGCGCY 1 cut(s) 28
BfuAI ACCTGC 1 cut(s) 318
BisI GCNGC 2 cut(s) 96, 99
BlsI GCNGC 2 cut(s) 97, 100
Bme1390I CCNGG 2 cut(s) 275, 280
Bme18I GGWCC 3 cut(s) 241, 297, 385
BmgBI CACGTC 2 cut(s) 226, 238
BmgT120I GGNCC 4 cut(s) 241, 276, 297, 385
BmiI GGNNCC 1 cut(s) 26
BmrFI CCNGG 2 cut(s) 275, 280
BmsI GCATC 2 cut(s) 14, 333
BpiI GAAGAC 1 cut(s) 402
BpuMI CCSGG 2 cut(s) 275, 280
Bsa29I ATCGAT 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 25
BsaJI CCNNGG 1 cut(s) 339
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse1I ACTGG 1 cut(s) 274
Bse21I CCTNAGG 2 cut(s) 20, 381
BseCI ATCGAT 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 339
BseLI CCNNNNNNNGG 1 cut(s) 316
BseNI ACTGG 1 cut(s) 274
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 82
BshFI GGCC 2 cut(s) 273, 278
BshNI GGYRCC 1 cut(s) 24
BshVI ATCGAT 1 cut(s) 330
BsiSI CCGG 4 cut(s) 206, 274, 279, 314
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 1 cut(s) 164
BsmBI CGTCTC 1 cut(s) 164
BsmI GAATGC 1 cut(s) 326
BsnI GGCC 2 cut(s) 273, 278
Bsp143I GATC 1 cut(s) 403
BspACI CCGC 1 cut(s) 99
BspANI GGCC 2 cut(s) 273, 278
BspDI ATCGAT 1 cut(s) 330
BspLI GGNNCC 1 cut(s) 26
BspMI ACCTGC 1 cut(s) 318
BspPI GGATC 1 cut(s) 411
BspT107I GGYRCC 1 cut(s) 24
BsrI ACTGG 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 339
BssMI GATC 1 cut(s) 403
BssNI GRCGYC 1 cut(s) 25
BssT1I CCWWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 404
BstACI GRCGYC 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 290, 362
BstDEI CTNAG 2 cut(s) 20, 381
BstENI CCTNNNNNAGG 1 cut(s) 314
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 1 cut(s) 27
BstKTI GATC 1 cut(s) 406
BstMAI GTCTC 1 cut(s) 164
BstMBI GATC 1 cut(s) 403
BstMWI GCNNNNNNNGC 2 cut(s) 144, 289
BstSCI CCNGG 2 cut(s) 273, 278
BstV1I GCAGC 1 cut(s) 82
BstV2I GAAGAC 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 330
Bsu36I CCTNAGG 2 cut(s) 20, 381
BsuRI GGCC 2 cut(s) 273, 278
BsuTUI ATCGAT 1 cut(s) 330
BtrI CACGTC 2 cut(s) 226, 238
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 267
BveI ACCTGC 1 cut(s) 318
Cac8I GCNNGC 2 cut(s) 290, 362
CfoI GCGC 1 cut(s) 27
Cfr13I GGNCC 4 cut(s) 241, 276, 297, 385
ClaI ATCGAT 1 cut(s) 330
Csp6I GTAC 1 cut(s) 160
CviAII CATG 2 cut(s) 34, 284
CviJI RGCY 5 cut(s) 95, 182, 273, 278, 420
CviKI_1 RGCY 5 cut(s) 95, 182, 273, 278, 420
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 2 cut(s) 20, 381
DinI GGCGCC 1 cut(s) 26
DpnI GATC 1 cut(s) 405
DpnII GATC 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 404
EarI CTCTTC 1 cut(s) 404
Eco130I CCWWGG 1 cut(s) 339
Eco47I GGWCC 3 cut(s) 241, 297, 385
Eco57I CTGAAG 1 cut(s) 386
Eco81I CCTNAGG 2 cut(s) 20, 381
EcoNI CCTNNNNNAGG 1 cut(s) 314
EcoO109I RGGNCCY 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 339
EcoT22I ATGCAT 1 cut(s) 326
EgeI GGCGCC 1 cut(s) 26
EheI GGCGCC 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 339
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 2 cut(s) 37, 287
FaiI YATR 6 cut(s) 35, 189, 213, 285, 306, 434
FatI CATG 2 cut(s) 33, 283
Fnu4HI GCNGC 2 cut(s) 96, 99
Fsp4HI GCNGC 2 cut(s) 96, 99
GlaI GCGC 1 cut(s) 26
GluI GCNGC 2 cut(s) 96, 99
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 273, 278
HapII CCGG 4 cut(s) 206, 274, 279, 314
HhaI GCGC 1 cut(s) 27
Hin1I GRCGYC 1 cut(s) 25
Hin1II CATG 2 cut(s) 37, 287
Hin6I GCGC 1 cut(s) 25
HinP1I GCGC 1 cut(s) 25
HindIII AAGCTT 2 cut(s) 180, 418
HinfI GANTC 1 cut(s) 67
HpaII CCGG 4 cut(s) 206, 274, 279, 314
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 4 cut(s) 162, 235, 241, 430
Hpy188I TCNGA 1 cut(s) 408
Hpy8I GTNNAC 4 cut(s) 162, 235, 241, 430
Hpy99I CGWCG 3 cut(s) 143, 146, 359
HpyAV CCTTC 2 cut(s) 223, 361
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4IV ACGT 2 cut(s) 225, 237
HpyCH4V TGCA 1 cut(s) 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 144, 289
HpyF3I CTNAG 2 cut(s) 20, 381
HpySE526I ACGT 2 cut(s) 225, 237
Hsp92I GRCGYC 1 cut(s) 25
Hsp92II CATG 2 cut(s) 37, 287
HspAI GCGC 1 cut(s) 25
KasI GGCGCC 1 cut(s) 24
Kzo9I GATC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 55, 127
Lsp1109I GCAGC 1 cut(s) 82
LweI GCATC 2 cut(s) 14, 333
MaeII ACGT 2 cut(s) 225, 237
MaeIII GTNAC 1 cut(s) 265
MalI GATC 1 cut(s) 405
MboI GATC 1 cut(s) 403
MboII GAAGA 2 cut(s) 407, 421
MluCI AATT 1 cut(s) 199
Mly113I GGCGCC 1 cut(s) 25
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 8 cut(s) 38, 41, 142, 186, 310, 310, 394, 398
Mph1103I ATGCAT 1 cut(s) 326
MspI CCGG 4 cut(s) 206, 274, 279, 314
MspR9I CCNGG 2 cut(s) 275, 280
Mva1269I GAATGC 1 cut(s) 326
MwoI GCNNNNNNNGC 2 cut(s) 144, 289
NarI GGCGCC 1 cut(s) 25
NciI CCSGG 2 cut(s) 275, 280
NdeII GATC 1 cut(s) 403
NlaIII CATG 2 cut(s) 37, 287
NlaIV GGNNCC 1 cut(s) 26
NmuCI GTSAC 1 cut(s) 265
NsiI ATGCAT 1 cut(s) 326
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 1 cut(s) 326
PkrI GCNGC 2 cut(s) 97, 100
PleI GAGTC 1 cut(s) 61
PluTI GGCGCC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 61
PpuMI RGGWCCY 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 385
PspN4I GGNNCC 1 cut(s) 26
PspPI GGNCC 4 cut(s) 241, 276, 297, 385
PspPPI RGGWCCY 1 cut(s) 385
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SatI GCNGC 2 cut(s) 96, 99
Sau3AI GATC 1 cut(s) 403
Sau96I GGNCC 4 cut(s) 241, 276, 297, 385
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 2 cut(s) 275, 280
SfaNI GCATC 2 cut(s) 14, 333
SfoI GGCGCC 1 cut(s) 26
SinI GGWCC 3 cut(s) 241, 297, 385
Sse9I AATT 1 cut(s) 199
SsiI CCGC 1 cut(s) 99
SspDI GGCGCC 1 cut(s) 24
StyD4I CCNGG 2 cut(s) 273, 278
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 1 cut(s) 265
TaiI ACGT 2 cut(s) 228, 240
TaqI TCGA 3 cut(s) 65, 330, 390
TaqII GACCGA 1 cut(s) 258
TasI AATT 1 cut(s) 199
TauI GCSGC 1 cut(s) 101
TscAI CASTG 2 cut(s) 124, 274
TseFI GTSAC 1 cut(s) 265
TseI GCWGC 1 cut(s) 95
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 3 cut(s) 100, 365, 408
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 2 cut(s) 124, 274
VpaK11BI GGWCC 3 cut(s) 241, 297, 385
XagI CCTNNNNNAGG 1 cut(s) 314
XapI RAATTY 1 cut(s) 199
Zsp2I ATGCAT 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.