Rh6CG072500

response to heat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0019198
Physical Location & Seq
Forward (+)
7401656 .. 7402443
788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG072500.1

Sequence Viewer

Length: 453 bp
ATGGCATCTTCACTAACCCTAAGGCGGCTCCTGTCCTCAAAACTTATGCCCAAATCCCTTGCTCCAATATCATCATCGACTCGCTCTATCACCACCACCCTGTCGGCTGCCTCCAAGTACTTCATCTCCAGTGCTCCTGCTGATTACGGCGACGACGGCGAGGGGGAGTGGTACGCGCAAGAGACGGAAAAAGCTTATTATGTCCGATTGAATTTGCATGAATATGAGAAAAAGGACGTGAAGGTGGACGTGAACCGAAAGGAGAACATTCTTACCGTCACTGGCCGGGCCGGGCATGGCAGGCATTGGACCTCTTATAACCTGCCGGAGGAATGCATTATCGATTACACCAAGGCAGAGATGAACGACGGTGTGCTGAAGGTTGTTGTGCCCAAGGTCCTTGATGAAGAAGAGGATCAGAAGAGTGAAGTTCTTAGTGTCAACATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

150

Amino Acids

16.86

Weight (kDa)

5.3

Isoelectric Point (pI)

52.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 60 - 141 1.7e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019198)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g05762
pyrus_communis pycom01g19310
rosa_laevigata RLG00000014742
rosa_multiflora Rmu_sc0007004.1_g000009
rosa_samantha Rh6AG083300 Rh6BG077900 Rh6CG072500 Rh6DG069700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 318
Acc36I ACCTGC 1 cut(s) 330
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 25
AclWI GGATC 1 cut(s) 423
AcoI YGGCCR 1 cut(s) 283
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 398
AfaI GTAC 2 cut(s) 119, 173
AfiI CCNNNNNNNGG 2 cut(s) 24, 328
AgsI TTSAA 1 cut(s) 211
AjiI CACGTC 2 cut(s) 238, 250
AluBI AGCT 1 cut(s) 194
AluI AGCT 1 cut(s) 194
Alw21I GWGCWC 1 cut(s) 136
Alw26I GTCTC 1 cut(s) 176
AlwI GGATC 1 cut(s) 423
AoxI GGCC 2 cut(s) 283, 288
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 211
AspLEI GCGC 1 cut(s) 178
AspS9I GGNCC 3 cut(s) 288, 309, 397
AsuC2I CCSGG 2 cut(s) 287, 292
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 2 cut(s) 309, 397
AxyI CCTNAGG 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 393
Bbv12I GWGCWC 1 cut(s) 136
BbvI GCAGC 1 cut(s) 94
BceAI ACGGC 2 cut(s) 163, 172
BcnI CCSGG 2 cut(s) 287, 292
BcoDI GTCTC 1 cut(s) 176
BfuAI ACCTGC 1 cut(s) 330
BisI GCNGC 2 cut(s) 26, 108
BlsI GCNGC 2 cut(s) 27, 109
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 2 cut(s) 287, 292
Bme18I GGWCC 2 cut(s) 309, 397
BmgBI CACGTC 2 cut(s) 238, 250
BmgT120I GGNCC 3 cut(s) 288, 309, 397
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 2 cut(s) 287, 292
BmsI GCATC 1 cut(s) 14
BpmI CTGGAG 1 cut(s) 112
BpuMI CCSGG 2 cut(s) 287, 292
Bsa29I ATCGAT 1 cut(s) 342
BsaJI CCNNGG 2 cut(s) 351, 393
BsaXI ACNNNNNCTCC 2 cut(s) 110, 140
Bsc4I CCNNNNNNNGG 2 cut(s) 24, 328
Bse1I ACTGG 2 cut(s) 129, 286
Bse21I CCTNAGG 1 cut(s) 20
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 2 cut(s) 351, 393
BseLI CCNNNNNNNGG 2 cut(s) 24, 328
BseNI ACTGG 2 cut(s) 129, 286
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 1 cut(s) 94
Bsh1236I CGCG 1 cut(s) 176
BshFI GGCC 2 cut(s) 285, 290
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 1 cut(s) 136
BsiSI CCGG 3 cut(s) 286, 291, 326
BslI CCNNNNNNNGG 2 cut(s) 24, 328
BsmAI GTCTC 1 cut(s) 176
BsmBI CGTCTC 1 cut(s) 176
BsmI GAATGC 1 cut(s) 338
BsnI GGCC 2 cut(s) 285, 290
Bsp1286I GDGCHC 2 cut(s) 136, 393
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 1 cut(s) 25
BspANI GGCC 2 cut(s) 285, 290
BspDI ATCGAT 1 cut(s) 342
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 29
BspMI ACCTGC 1 cut(s) 330
BspPI GGATC 1 cut(s) 423
BsrI ACTGG 2 cut(s) 129, 286
BssECI CCNNGG 2 cut(s) 351, 393
BssMI GATC 1 cut(s) 415
BssT1I CCWWGG 2 cut(s) 351, 393
Bst4CI ACNGT 2 cut(s) 277, 371
Bst6I CTCTTC 2 cut(s) 405, 416
BstC8I GCNNGC 1 cut(s) 302
BstDEI CTNAG 2 cut(s) 20, 434
BstENI CCTNNNNNAGG 1 cut(s) 326
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 1 cut(s) 178
BstKTI GATC 1 cut(s) 418
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 2 cut(s) 156, 301
BstSCI CCNGG 2 cut(s) 285, 290
BstSLI GKGCMC 1 cut(s) 393
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 94
Bsu15I ATCGAT 1 cut(s) 342
Bsu36I CCTNAGG 1 cut(s) 20
BsuRI GGCC 2 cut(s) 285, 290
BsuTUI ATCGAT 1 cut(s) 342
BtrI CACGTC 2 cut(s) 238, 250
BtsIMutI CAGTG 2 cut(s) 136, 279
BveI ACCTGC 1 cut(s) 330
Cac8I GCNNGC 1 cut(s) 302
CfoI GCGC 1 cut(s) 178
Cfr13I GGNCC 3 cut(s) 288, 309, 397
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 2 cut(s) 118, 172
CviAII CATG 2 cut(s) 218, 296
CviJI RGCY 5 cut(s) 28, 107, 194, 285, 290
CviKI_1 RGCY 5 cut(s) 28, 107, 194, 285, 290
CviQI GTAC 2 cut(s) 118, 172
DdeI CTNAG 2 cut(s) 20, 434
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 283
Eam1104I CTCTTC 2 cut(s) 405, 416
EarI CTCTTC 2 cut(s) 405, 416
Eco130I CCWWGG 2 cut(s) 351, 393
Eco47I GGWCC 2 cut(s) 309, 397
Eco57I CTGAAG 1 cut(s) 398
Eco81I CCTNAGG 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 326
EcoO109I RGGNCCY 1 cut(s) 397
EcoT14I CCWWGG 2 cut(s) 351, 393
EcoT22I ATGCAT 1 cut(s) 338
ErhI CCWWGG 2 cut(s) 351, 393
Esp3I CGTCTC 1 cut(s) 176
FaeI CATG 2 cut(s) 221, 299
FaiI YATR 7 cut(s) 47, 201, 219, 225, 297, 318, 446
FatI CATG 2 cut(s) 217, 295
Fnu4HI GCNGC 2 cut(s) 26, 108
Fsp4HI GCNGC 2 cut(s) 26, 108
GlaI GCGC 1 cut(s) 177
GluI GCNGC 2 cut(s) 26, 108
GsuI CTGGAG 1 cut(s) 112
HaeIII GGCC 2 cut(s) 285, 290
HapII CCGG 3 cut(s) 286, 291, 326
HhaI GCGC 1 cut(s) 178
Hin1II CATG 2 cut(s) 221, 299
Hin6I GCGC 1 cut(s) 176
HinP1I GCGC 1 cut(s) 176
HincII GTYRAC 1 cut(s) 442
HindII GTYRAC 1 cut(s) 442
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 1 cut(s) 79
HpaII CCGG 3 cut(s) 286, 291, 326
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 3 cut(s) 247, 253, 442
Hpy188I TCNGA 2 cut(s) 206, 420
Hpy8I GTNNAC 3 cut(s) 247, 253, 442
Hpy99I CGWCG 3 cut(s) 155, 158, 371
HpyAV CCTTC 2 cut(s) 235, 373
HpyCH4III ACNGT 2 cut(s) 277, 371
HpyCH4IV ACGT 2 cut(s) 237, 249
HpyCH4V TGCA 2 cut(s) 217, 336
HpyF10VI GCNNNNNNNGC 2 cut(s) 156, 301
HpyF3I CTNAG 2 cut(s) 20, 434
HpySE526I ACGT 2 cut(s) 237, 249
Hsp92II CATG 2 cut(s) 221, 299
HspAI GCGC 1 cut(s) 176
Kzo9I GATC 1 cut(s) 415
LmnI GCTCC 3 cut(s) 33, 67, 139
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 1 cut(s) 14
MaeII ACGT 2 cut(s) 237, 249
MaeIII GTNAC 1 cut(s) 277
MalI GATC 1 cut(s) 417
MboI GATC 1 cut(s) 415
MboII GAAGA 3 cut(s) 419, 422, 433
MhlI GDGCHC 2 cut(s) 136, 393
MluCI AATT 1 cut(s) 211
MlyI GAGTC 1 cut(s) 73
MnlI CCTC 6 cut(s) 46, 121, 154, 322, 322, 406
Mph1103I ATGCAT 1 cut(s) 338
MslI CAYNNNNRTG 1 cut(s) 222
MspI CCGG 3 cut(s) 286, 291, 326
MspR9I CCNGG 2 cut(s) 287, 292
Mva1269I GAATGC 1 cut(s) 338
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 2 cut(s) 156, 301
NciI CCSGG 2 cut(s) 287, 292
NdeII GATC 1 cut(s) 415
NlaIII CATG 2 cut(s) 221, 299
NlaIV GGNNCC 1 cut(s) 29
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 338
PctI GAATGC 1 cut(s) 338
PkrI GCNGC 2 cut(s) 27, 109
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 397
PsiI TTATAA 1 cut(s) 318
Psp5II RGGWCCY 1 cut(s) 397
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 3 cut(s) 288, 309, 397
PspPPI RGGWCCY 1 cut(s) 397
RsaI GTAC 2 cut(s) 119, 173
RsaNI GTAC 2 cut(s) 118, 172
RseI CAYNNNNRTG 1 cut(s) 222
SatI GCNGC 2 cut(s) 26, 108
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 3 cut(s) 288, 309, 397
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 1 cut(s) 73
ScrFI CCNGG 2 cut(s) 287, 292
SduI GDGCHC 2 cut(s) 136, 393
SetI ASST 8 cut(s) 196, 240, 246, 252, 314, 324, 384, 399
SfaNI GCATC 1 cut(s) 14
SinI GGWCC 2 cut(s) 309, 397
SmiMI CAYNNNNRTG 1 cut(s) 222
Sse9I AATT 1 cut(s) 211
SsiI CCGC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 285, 290
StyI CCWWGG 2 cut(s) 351, 393
TaaI ACNGT 2 cut(s) 277, 371
TaiI ACGT 2 cut(s) 240, 252
TaqI TCGA 2 cut(s) 77, 342
TasI AATT 1 cut(s) 211
TatI WGTACW 1 cut(s) 117
TauI GCSGC 1 cut(s) 28
TscAI CASTG 2 cut(s) 136, 286
TseFI GTSAC 1 cut(s) 277
TseI GCWGC 1 cut(s) 107
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 112, 234, 377, 420
TspGWI ACGGA 1 cut(s) 200
TspRI CASTG 2 cut(s) 136, 286
VpaK11BI GGWCC 2 cut(s) 309, 397
XagI CCTNNNNNAGG 1 cut(s) 326
XapI RAATTY 1 cut(s) 211
ZrmI AGTACT 1 cut(s) 119
Zsp2I ATGCAT 1 cut(s) 338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.