FvH4_2g10300

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0016257
Physical Location & Seq
Forward (+)
9139490 .. 9141393
1904 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10300.t1

Sequence Viewer

Length: 1386 bp
ATGTTGGACCAGCTCACGCACCGAGGGCTGGCCCAACTTGGCAAACTATATGGCGGTCTTGTCCATCTCCAAATAGGCGTGCTACATATCATGGCTGTATCAACCCCTGAAATGGCACGCGAAATCCTCCAAGCCCAAGACAGTGTCTTTGCTAATCGGCCCGCCAATGTTACAATTGCCTACTTGACATACGACCGAGCCGACATGGCGTTTGCCAACTACGGCCCTTTTTGGCGTCGCATGCGTAAAATTTGTGTCATGAATATATTTAGCCGAAAACGGGCCGAATCATGGGCCTCGGTACGTGAGGAAATCGATGAAACTGTTCGGACTGTGGCGGAAAAAGTTGGTTCACCAGTAAACATTGGGGAGTTGGTTTTCGCCCTCACACGAAACATTACCTACCGAGCTGCGTTTGGCTCAAAATCGCATGAAGGGCAAGACGAATTTATGAAGATTTTGCAAGAATTTTCAAAGCTTTTTGGAGCATTCAATATGCAAGATTTCCTACCATGGTTGGGTTGGATGCATGGACAAGCATTCCAAAATAGAATGGCCAAAGCTCGCAAATCGTTAGATGTGTTCATCGATAAGATTATCGATGACCACATGACTAAGAAGACTACTAAGGAAAATGGCATTGAGGCTGAGACAGATATGGTGGATGAATTACTCGCTTTTATTAGAAATGATGCTTCAATGGACATTGATGACTCCAATTCTGGCTTTGCCCTCACCAGAGATAATATCAAGGCGCTCGTAATGGATGTCATGTTTGGTGGAACTGAAACAGTTGCGTCAGTAATCGAATGGACCCTTGCAGAGCTTATGAAGAGTCCACAAGATCTCATAAAGGTTCAACAAGAGCTCGTTGATGTTGTAGGGTTGAACCGTAGAGTGCAAGAAACCGACCTGGAAAACCTAACCTACCTCAAATGTGCAGTCAAGGAAGCCCTCCGTCTCCACCCACCAATCCCTCTCCTCCTCCACGAGGCCGCCGAGGACGGCGTCGTGGCCGGGTACTCATTCCCCGCAGGATCACGGGTCTACATCAACGCGTGGGCGATAGGGCGTGACCCTTCTGCATGGGACGAGCCTGACAAGTTCAAACCCTCAAGGTTTCTGAAAGATGGCTCCCCGGATTTCAAAGGGAATGACTTCGAGTTCATCCCATTCGGGTCCGGCAGGAGGTCTTGTCCGGGCATGGCGCTAGGGCTTTATGGGTTGGAGATGGCTGTGGCTCATCTGCTTCATTGTTTTACGTGGGAGTTGCCGGATGGGATGAAGGCTAGTGAGCTTGACATGAACGACGTGTTTGGATTGACTGCACCAAGAGCTGTTCAGCTCGTTGCTGTGCCACACTACAGGTTGAACTGCCCGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00940 ko01100 ko01110 map00940 map01100 map01110
Pfam Domains
Protein Families
p450

Protein Analysis

462

Amino Acids

51.81

Weight (kDa)

5.56

Isoelectric Point (pI)

39.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 7 - 443 7.2e-93 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016257)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g10300
malus_domestica MD10G1064200.v1.1
prunus_persica Prupe.8G085900_v2.0.a1
pyrus_communis pycom10g04980 pycom10g05010
rosa_chinensis RchiOBHm_Chr6g0267501
rosa_laevigata RLG00000013998
rosa_multiflora Rmu_sc0012005.1_g000011
rosa_roxburghii Rroxscaffold_7G00200690
rosa_rugosa Rorug06G0033100
rosa_samantha Rh6AG154600 Rh6BG155200 Rh6CG150500 Rh6DG140400
rosa_wichuraiana Rw6G013270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1047
AccII CGCG 2 cut(s) 120, 1058
AciI CCGC 6 cut(s) 54, 162, 338, 996, 1032, 1379
AclWI GGATC 1 cut(s) 1045
AcoI YGGCCR 2 cut(s) 555, 1014
AcsI RAATTY 3 cut(s) 249, 446, 467
AcyI GRCGYC 2 cut(s) 235, 1008
AfaI GTAC 2 cut(s) 303, 1022
AfiI CCNNNNNNNGG 7 cut(s) 28, 112, 280, 291, 518, 991, 1188
AflIII ACRYGT 2 cut(s) 1056, 1311
AgsI TTSAA 8 cut(s) 474, 493, 699, 860, 889, 1108, 1147, 1372
AjiI CACGTC 1 cut(s) 1312
AjnI CCWGG 1 cut(s) 912
AluBI AGCT 9 cut(s) 13, 410, 478, 563, 826, 868, 1297, 1337, 1345
AluI AGCT 9 cut(s) 13, 410, 478, 563, 826, 868, 1297, 1337, 1345
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 2 cut(s) 644, 965
AlwI GGATC 1 cut(s) 1045
AoxI GGCC 8 cut(s) 30, 158, 223, 282, 294, 555, 993, 1014
ApeKI GCWGC 1 cut(s) 410
ApoI RAATTY 3 cut(s) 249, 446, 467
ArsI GACNNNNNNTTYG 6 cut(s) 131, 163, 194, 226, 927, 959
Asp700I GAANNNNTTC 2 cut(s) 324, 1157
AspLEI GCGC 2 cut(s) 757, 1210
AspS9I GGNCC 8 cut(s) 7, 31, 159, 224, 282, 294, 813, 1179
AsuC2I CCSGG 3 cut(s) 1018, 1139, 1200
AsuHPI GGTGA 2 cut(s) 345, 727
AvaII GGWCC 3 cut(s) 7, 813, 1179
BalI TGGCCA 1 cut(s) 557
BanII GRGCYC 1 cut(s) 870
BauI CACGAG 1 cut(s) 989
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 1 cut(s) 397
BccI CCATC 4 cut(s) 72, 1124, 1225, 1271
BceAI ACGGC 2 cut(s) 238, 1021
BciT130I CCWGG 1 cut(s) 914
BcnI CCSGG 3 cut(s) 1018, 1139, 1200
BcoDI GTCTC 2 cut(s) 644, 965
BfaI CTAG 2 cut(s) 1211, 1290
BfmI CTRYAG 1 cut(s) 1363
BfoI RGCGCY 2 cut(s) 758, 1211
BglI GCCNNNNNGGC 1 cut(s) 206
BglII AGATCT 1 cut(s) 844
BisI GCNGC 2 cut(s) 411, 996
BlsI GCNGC 2 cut(s) 412, 997
Bme1390I CCNGG 4 cut(s) 914, 1018, 1139, 1200
Bme18I GGWCC 3 cut(s) 7, 813, 1179
BmgBI CACGTC 1 cut(s) 1312
BmgT120I GGNCC 8 cut(s) 7, 31, 159, 224, 282, 294, 813, 1179
BmiI GGNNCC 3 cut(s) 815, 1135, 1180
BmrFI CCNGG 4 cut(s) 914, 1018, 1139, 1200
BmsI GCATC 2 cut(s) 516, 682
BpiI GAAGAC 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 1099
BpuMI CCSGG 3 cut(s) 1018, 1139, 1200
Bsa29I ATCGAT 3 cut(s) 315, 588, 600
BsaAI YACGTR 2 cut(s) 305, 1263
BsaHI GRCGYC 2 cut(s) 235, 1008
BsaJI CCNNGG 5 cut(s) 22, 297, 512, 999, 1137
BsaXI ACNNNNNCTCC 2 cut(s) 1180, 1210
Bsc4I CCNNNNNNNGG 7 cut(s) 28, 112, 280, 291, 518, 991, 1188
Bse1I ACTGG 1 cut(s) 356
BseBI CCWGG 1 cut(s) 914
BseCI ATCGAT 3 cut(s) 315, 588, 600
BseDI CCNNGG 5 cut(s) 22, 297, 512, 999, 1137
BseGI GGATG 6 cut(s) 531, 670, 772, 1167, 1282, 1287
BseLI CCNNNNNNNGG 7 cut(s) 28, 112, 280, 291, 518, 991, 1188
BseMII CTCAG 1 cut(s) 639
BseNI ACTGG 1 cut(s) 356
BseRI GAGGAG 2 cut(s) 971, 974
BseXI GCAGC 1 cut(s) 397
BsgI GTGCAG 2 cut(s) 960, 1311
Bsh1236I CGCG 2 cut(s) 120, 1058
Bsh1285I CGRYCG 1 cut(s) 196
BshFI GGCC 8 cut(s) 32, 160, 225, 284, 296, 557, 995, 1016
BshVI ATCGAT 3 cut(s) 315, 588, 600
BsiEI CGRYCG 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 870
BsiSI CCGG 5 cut(s) 1017, 1139, 1182, 1199, 1274
BslFI GGGAC 1 cut(s) 1103
BslI CCNNNNNNNGG 7 cut(s) 28, 112, 280, 291, 518, 991, 1188
BsmAI GTCTC 2 cut(s) 644, 965
BsmBI CGTCTC 1 cut(s) 965
BsmFI GGGAC 1 cut(s) 1103
BsmI GAATGC 2 cut(s) 488, 539
BsnI GGCC 8 cut(s) 32, 160, 225, 284, 296, 557, 995, 1016
Bsp1286I GDGCHC 1 cut(s) 870
Bsp143I GATC 2 cut(s) 844, 1037
Bsp19I CCATGG 1 cut(s) 512
BspACI CCGC 6 cut(s) 54, 162, 338, 996, 1032, 1379
BspANI GGCC 8 cut(s) 32, 160, 225, 284, 296, 557, 995, 1016
BspCNI CTCAG 1 cut(s) 640
BspDI ATCGAT 3 cut(s) 315, 588, 600
BspFNI CGCG 2 cut(s) 120, 1058
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 3 cut(s) 815, 1135, 1180
BspPI GGATC 1 cut(s) 1045
BsrI ACTGG 1 cut(s) 356
BssECI CCNNGG 5 cut(s) 22, 297, 512, 999, 1137
BssMI GATC 2 cut(s) 844, 1037
BssNI GRCGYC 2 cut(s) 235, 1008
BssSI CACGAG 1 cut(s) 989
BssT1I CCWWGG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 989
Bst2UI CCWGG 1 cut(s) 914
Bst4CI ACNGT 5 cut(s) 143, 325, 334, 793, 893
Bst6I CTCTTC 1 cut(s) 827
BstACI GRCGYC 2 cut(s) 235, 1008
BstBAI YACGTR 2 cut(s) 305, 1263
BstC8I GCNNGC 7 cut(s) 30, 80, 118, 162, 242, 565, 1379
BstDEI CTNAG 3 cut(s) 615, 627, 648
BstDSI CCRYGG 1 cut(s) 512
BstENI CCTNNNNNAGG 1 cut(s) 989
BstF5I GGATG 6 cut(s) 531, 670, 772, 1167, 1282, 1287
BstFNI CGCG 2 cut(s) 120, 1058
BstH2I RGCGCY 2 cut(s) 758, 1211
BstHHI GCGC 2 cut(s) 757, 1210
BstKTI GATC 2 cut(s) 847, 1040
BstMAI GTCTC 2 cut(s) 644, 965
BstMBI GATC 2 cut(s) 844, 1037
BstMCI CGRYCG 1 cut(s) 196
BstMWI GCNNNNNNNGC 5 cut(s) 25, 206, 241, 436, 1334
BstNI CCWGG 1 cut(s) 914
BstNSI RCATGY 1 cut(s) 244
BstSCI CCNGG 4 cut(s) 912, 1016, 1137, 1198
BstSFI CTRYAG 1 cut(s) 1363
BstUI CGCG 2 cut(s) 120, 1058
BstV1I GCAGC 1 cut(s) 397
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 1 cut(s) 844
BstYI RGATCY 1 cut(s) 844
Bsu15I ATCGAT 3 cut(s) 315, 588, 600
BsuRI GGCC 8 cut(s) 32, 160, 225, 284, 296, 557, 995, 1016
BsuTUI ATCGAT 3 cut(s) 315, 588, 600
BtgI CCRYGG 1 cut(s) 512
BtrI CACGTC 1 cut(s) 1312
BtsCI GGATG 6 cut(s) 531, 670, 772, 1167, 1282, 1287
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 7 cut(s) 30, 80, 118, 162, 242, 565, 1379
CciI TCATGA 1 cut(s) 258
CfoI GCGC 2 cut(s) 757, 1210
Cfr13I GGNCC 8 cut(s) 7, 31, 159, 224, 282, 294, 813, 1179
ClaI ATCGAT 3 cut(s) 315, 588, 600
CseI GACGC 3 cut(s) 224, 786, 997
Csp6I GTAC 2 cut(s) 302, 1021
CviQI GTAC 2 cut(s) 302, 1021
DdeI CTNAG 3 cut(s) 615, 627, 648
DpnI GATC 2 cut(s) 846, 1039
DpnII GATC 2 cut(s) 844, 1037
EaeI YGGCCR 2 cut(s) 555, 1014
Eam1104I CTCTTC 1 cut(s) 827
EarI CTCTTC 1 cut(s) 827
EciI GGCGGA 1 cut(s) 353
Ecl136II GAGCTC 1 cut(s) 868
Eco130I CCWWGG 1 cut(s) 512
Eco24I GRGCYC 1 cut(s) 870
Eco47I GGWCC 3 cut(s) 7, 813, 1179
Eco53kI GAGCTC 1 cut(s) 868
EcoICRI GAGCTC 1 cut(s) 868
EcoNI CCTNNNNNAGG 1 cut(s) 989
EcoRII CCWGG 1 cut(s) 912
EcoT14I CCWWGG 1 cut(s) 512
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 512
Esp3I CGTCTC 1 cut(s) 965
FaqI GGGAC 1 cut(s) 1103
FauI CCCGC 2 cut(s) 169, 1039
FblI GTMKAC 1 cut(s) 1047
Fnu4HI GCNGC 2 cut(s) 411, 996
FokI GGATG 6 cut(s) 538, 677, 779, 1154, 1289, 1294
FriOI GRGCYC 1 cut(s) 870
Fsp4HI GCNGC 2 cut(s) 411, 996
FspBI CTAG 2 cut(s) 1211, 1290
GlaI GCGC 2 cut(s) 756, 1209
GluI GCNGC 2 cut(s) 411, 996
HaeII RGCGCY 2 cut(s) 758, 1211
HaeIII GGCC 8 cut(s) 32, 160, 225, 284, 296, 557, 995, 1016
HapII CCGG 5 cut(s) 1017, 1139, 1182, 1199, 1274
HgaI GACGC 3 cut(s) 224, 786, 997
HhaI GCGC 2 cut(s) 757, 1210
Hin1I GRCGYC 2 cut(s) 235, 1008
Hin6I GCGC 2 cut(s) 755, 1208
HinP1I GCGC 2 cut(s) 755, 1208
HindIII AAGCTT 1 cut(s) 476
HinfI GANTC 3 cut(s) 287, 713, 835
HpaII CCGG 5 cut(s) 1017, 1139, 1182, 1199, 1274
HphI GGTGA 2 cut(s) 345, 727
Hpy166II GTNNAC 4 cut(s) 353, 361, 839, 1048
Hpy188I TCNGA 2 cut(s) 330, 1125
Hpy188III TCNNGA 1 cut(s) 259
Hpy8I GTNNAC 4 cut(s) 353, 361, 839, 1048
Hpy99I CGWCG 3 cut(s) 240, 1013, 1313
HpyAV CCTTC 3 cut(s) 428, 1089, 1279
HpyCH4III ACNGT 5 cut(s) 143, 325, 334, 793, 893
HpyCH4IV ACGT 3 cut(s) 304, 1262, 1311
HpyCH4V TGCA 8 cut(s) 463, 499, 529, 821, 901, 941, 1085, 1328
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 206, 241, 436, 1334
HpyF3I CTNAG 3 cut(s) 615, 627, 648
HpySE526I ACGT 3 cut(s) 304, 1262, 1311
Hsp92I GRCGYC 2 cut(s) 235, 1008
HspAI GCGC 2 cut(s) 755, 1208
Kzo9I GATC 2 cut(s) 844, 1037
LmnI GCTCC 2 cut(s) 485, 1139
Lsp1109I GCAGC 1 cut(s) 397
LweI GCATC 2 cut(s) 516, 682
MaeI CTAG 2 cut(s) 1211, 1290
MaeII ACGT 3 cut(s) 304, 1262, 1311
MaeIII GTNAC 2 cut(s) 169, 1073
MalI GATC 2 cut(s) 846, 1039
MboI GATC 2 cut(s) 844, 1037
MboII GAAGA 3 cut(s) 466, 631, 844
MfeI CAATTG 1 cut(s) 174
MflI RGATCY 1 cut(s) 844
MhlI GDGCHC 1 cut(s) 870
MlsI TGGCCA 1 cut(s) 557
MluCI AATT 6 cut(s) 174, 249, 446, 467, 668, 718
MluI ACGCGT 1 cut(s) 1056
MluNI TGGCCA 1 cut(s) 557
MlyI GAGTC 2 cut(s) 707, 844
MmeI TCCRAC 2 cut(s) 503, 1206
Mox20I TGGCCA 1 cut(s) 557
Mph1103I ATGCAT 1 cut(s) 531
MroXI GAANNNNTTC 2 cut(s) 324, 1157
MscI TGGCCA 1 cut(s) 557
Msp20I TGGCCA 1 cut(s) 557
MspI CCGG 5 cut(s) 1017, 1139, 1182, 1199, 1274
MspR9I CCNGG 4 cut(s) 914, 1018, 1139, 1200
MunI CAATTG 1 cut(s) 174
Mva1269I GAATGC 2 cut(s) 488, 539
MvaI CCWGG 1 cut(s) 914
MvnI CGCG 2 cut(s) 120, 1058
MwoI GCNNNNNNNGC 5 cut(s) 25, 206, 241, 436, 1334
NciI CCSGG 3 cut(s) 1018, 1139, 1200
NcoI CCATGG 1 cut(s) 512
NdeII GATC 2 cut(s) 844, 1037
NlaIV GGNNCC 3 cut(s) 815, 1135, 1180
NmeAIII GCCGAG 1 cut(s) 1024
NmuCI GTSAC 1 cut(s) 1073
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 244
PaeI GCATGC 1 cut(s) 244
PagI TCATGA 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 996
PctI GAATGC 2 cut(s) 488, 539
PdmI GAANNNNTTC 2 cut(s) 324, 1157
PfeI GAWTC 1 cut(s) 287
PflFI GACNNNGTC 2 cut(s) 143, 1007
PkrI GCNGC 2 cut(s) 412, 997
PleI GAGTC 2 cut(s) 707, 843
PpsI GAGTC 2 cut(s) 707, 843
Ppu21I YACGTR 2 cut(s) 305, 1263
Psp124BI GAGCTC 1 cut(s) 870
Psp6I CCWGG 1 cut(s) 912
PspGI CCWGG 1 cut(s) 912
PspN4I GGNNCC 3 cut(s) 815, 1135, 1180
PspPI GGNCC 8 cut(s) 7, 31, 159, 224, 282, 294, 813, 1179
PsuI RGATCY 1 cut(s) 844
PsyI GACNNNGTC 2 cut(s) 143, 1007
RsaI GTAC 2 cut(s) 303, 1022
RsaNI GTAC 2 cut(s) 302, 1021
SacI GAGCTC 1 cut(s) 870
SatI GCNGC 2 cut(s) 411, 996
Sau3AI GATC 2 cut(s) 844, 1037
Sau96I GGNCC 8 cut(s) 7, 31, 159, 224, 282, 294, 813, 1179
SchI GAGTC 2 cut(s) 707, 844
ScrFI CCNGG 4 cut(s) 914, 1018, 1139, 1200
SduI GDGCHC 1 cut(s) 870
SfaNI GCATC 2 cut(s) 516, 682
SfcI CTRYAG 1 cut(s) 1363
SinI GGWCC 3 cut(s) 7, 813, 1179
SmlI CTYRAG 1 cut(s) 1114
SmoI CTYRAG 1 cut(s) 1114
SphI GCATGC 1 cut(s) 244
Sse9I AATT 6 cut(s) 174, 249, 446, 467, 668, 718
SsiI CCGC 6 cut(s) 54, 162, 338, 996, 1032, 1379
SspMI CTAG 2 cut(s) 1211, 1290
SstI GAGCTC 1 cut(s) 870
StyD4I CCNGG 4 cut(s) 912, 1016, 1137, 1198
StyI CCWWGG 1 cut(s) 512
TaaI ACNGT 5 cut(s) 143, 325, 334, 793, 893
TaiI ACGT 3 cut(s) 307, 1265, 1314
TaqI TCGA 5 cut(s) 315, 588, 600, 807, 1161
TaqII GACCGA 1 cut(s) 210
TasI AATT 6 cut(s) 174, 249, 446, 467, 668, 718
TauI GCSGC 1 cut(s) 998
TfiI GAWTC 1 cut(s) 287
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 1073
TseI GCWGC 1 cut(s) 410
Tsp45I GTSAC 1 cut(s) 1073
TspGWI ACGGA 1 cut(s) 947
TspRI CASTG 1 cut(s) 148
Tth111I GACNNNGTC 2 cut(s) 143, 1007
VpaK11BI GGWCC 3 cut(s) 7, 813, 1179
XagI CCTNNNNNAGG 1 cut(s) 989
XapI RAATTY 3 cut(s) 249, 446, 467
XceI RCATGY 1 cut(s) 244
XcmI CCANNNNNNNNNTGG 1 cut(s) 519
XmiI GTMKAC 1 cut(s) 1047
XmnI GAANNNNTTC 2 cut(s) 324, 1157
XspI CTAG 2 cut(s) 1211, 1290
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.