FvH4_2g22411

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0024041
Physical Location & Seq
Forward (+)
18375354 .. 18376000
647 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g22411.t1

Sequence Viewer

Length: 498 bp
ATGAGGGGCTTTGCTTTTAAGCACCTCTGTTTTCAGTCCAAAAGCCAAAGCCAGCTATCAGGTGCTTCTGAAAGCCTGAAGCGCTTCCAAAGCACTGTGGCGCTGCTCTCTTTCCGTTCTCTCTTCTCTCTCTGCCCTTCGTTTTCGCTCACCACTTTCAGTCCTTCAGGGAAGCTTGTGCAGATCGAGCACGCTTTGACGGCAGTCGGGTCGGGTCAGACCTCTTTGGGTATCAAAGGTAATGTTGCTGCTCCGGGTTTTTACCTTAAGCTTGTGAGAGCTAGAGTTTATAGGCTTTGTGATTTGATGGGTGAGCTGGTGTTTAGCCTCTGCAGTCCTCCCCTAAATGCCCAGGTTGTCAAACACAGTCCATCTATTGTTCCAGACTTCCAGTTGCTACCATCTATTTGGCCCTATTTATACTGCTATATGCTATACCTGCAGTTTGATTCTCACAATCCTTTCCGCTGTGTGGCACCTTTCATTTACTTCATTTGA

Protein Analysis

166

Amino Acids

18.42

Weight (kDa)

9.39

Isoelectric Point (pI)

50.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome_A_N PF10584 48 - 68 8.2e-08 Proteasome subunit A N-terminal signature
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024041)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22411 FvH4_2g31320 FvH4_7g08971

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 475
AciI CCGC 1 cut(s) 466
AcuI CTGAAG 2 cut(s) 98, 150
AfeI AGCGCT 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 472
AflII CTTAAG 1 cut(s) 266
AjnI CCWGG 1 cut(s) 351
AluBI AGCT 5 cut(s) 55, 175, 271, 281, 316
AluI AGCT 5 cut(s) 55, 175, 271, 281, 316
Alw21I GWGCWC 1 cut(s) 192
Aor51HI AGCGCT 1 cut(s) 83
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 2 cut(s) 103, 248
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 2 cut(s) 84, 103
AspS9I GGNCC 1 cut(s) 411
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 2 cut(s) 142, 323
BanI GGYRCC 1 cut(s) 475
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 90, 235
BccI CCATC 3 cut(s) 301, 379, 409
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciT130I CCWGG 1 cut(s) 353
BcnI CCSGG 1 cut(s) 255
BfaI CTAG 1 cut(s) 282
BfmI CTRYAG 2 cut(s) 331, 440
BfoI RGCGCY 2 cut(s) 85, 104
BfrI CTTAAG 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 447
BisI GCNGC 2 cut(s) 104, 249
BlsI GCNGC 2 cut(s) 105, 250
Bme1390I CCNGG 2 cut(s) 255, 353
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 1 cut(s) 477
BmrFI CCNGG 2 cut(s) 255, 353
BoxI GACNNNNGTC 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 255
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 1 cut(s) 472
Bse1I ACTGG 1 cut(s) 391
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 472
BseNI ACTGG 1 cut(s) 391
BseXI GCAGC 2 cut(s) 90, 235
BsgI GTGCAG 1 cut(s) 200
BshFI GGCC 1 cut(s) 412
BshNI GGYRCC 1 cut(s) 475
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 472
BsnI GGCC 1 cut(s) 412
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 1 cut(s) 183
BspACI CCGC 1 cut(s) 466
BspANI GGCC 1 cut(s) 412
BspLI GGNNCC 1 cut(s) 477
BspMAI CTGCAG 2 cut(s) 335, 444
BspMI ACCTGC 1 cut(s) 447
BspT107I GGYRCC 1 cut(s) 475
BspTI CTTAAG 1 cut(s) 266
BsrI ACTGG 1 cut(s) 391
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 353
Bst4CI ACNGT 2 cut(s) 97, 368
Bst6I CTCTTC 1 cut(s) 128
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 53, 192
BstH2I RGCGCY 2 cut(s) 85, 104
BstHHI GCGC 2 cut(s) 84, 103
BstKTI GATC 1 cut(s) 186
BstMBI GATC 1 cut(s) 183
BstMWI GCNNNNNNNGC 5 cut(s) 81, 90, 187, 200, 439
BstNI CCWGG 1 cut(s) 353
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 2 cut(s) 253, 351
BstSFI CTRYAG 2 cut(s) 331, 440
BstV1I GCAGC 2 cut(s) 90, 235
BstXI CCANNNNNNTGG 1 cut(s) 408
BsuRI GGCC 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 93
BveI ACCTGC 1 cut(s) 447
Cac8I GCNNGC 2 cut(s) 53, 192
CfoI GCGC 2 cut(s) 84, 103
Cfr13I GGNCC 1 cut(s) 411
DpnI GATC 1 cut(s) 185
DpnII GATC 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
Eco47III AGCGCT 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 98, 150
EcoRII CCWGG 1 cut(s) 351
FaiI YATR 5 cut(s) 291, 421, 429, 431, 436
Fnu4HI GCNGC 2 cut(s) 104, 249
Fsp4HI GCNGC 2 cut(s) 104, 249
FspBI CTAG 1 cut(s) 282
GlaI GCGC 2 cut(s) 83, 102
GluI GCNGC 2 cut(s) 104, 249
HaeII RGCGCY 2 cut(s) 85, 104
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 254
HhaI GCGC 2 cut(s) 84, 103
Hin6I GCGC 2 cut(s) 82, 101
HinP1I GCGC 2 cut(s) 82, 101
HindIII AAGCTT 2 cut(s) 173, 269
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 142, 323
Hpy188I TCNGA 2 cut(s) 70, 219
Hpy188III TCNNGA 1 cut(s) 383
HpyAV CCTTC 2 cut(s) 147, 174
HpyCH4III ACNGT 2 cut(s) 97, 368
HpyCH4V TGCA 3 cut(s) 181, 333, 442
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 90, 187, 200, 439
HspAI GCGC 2 cut(s) 82, 101
Kzo9I GATC 1 cut(s) 183
LmnI GCTCC 1 cut(s) 256
Lsp1109I GCAGC 2 cut(s) 90, 235
MaeI CTAG 1 cut(s) 282
MalI GATC 1 cut(s) 185
MboI GATC 1 cut(s) 183
MboII GAAGA 1 cut(s) 115
MhlI GDGCHC 1 cut(s) 192
MnlI CCTC 4 cut(s) 35, 232, 338, 348
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 2 cut(s) 18, 267
MspA1I CMGCKG 1 cut(s) 468
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 2 cut(s) 255, 353
MvaI CCWGG 1 cut(s) 353
MwoI GCNNNNNNNGC 5 cut(s) 81, 90, 187, 200, 439
NciI CCSGG 1 cut(s) 255
NdeII GATC 1 cut(s) 183
NlaIV GGNNCC 1 cut(s) 477
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 449
PkrI GCNGC 2 cut(s) 105, 250
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspN4I GGNNCC 1 cut(s) 477
PspPI GGNCC 1 cut(s) 411
PstI CTGCAG 2 cut(s) 335, 444
SaqAI TTAA 2 cut(s) 18, 267
SatI GCNGC 2 cut(s) 104, 249
Sau3AI GATC 1 cut(s) 183
Sau96I GGNCC 1 cut(s) 411
ScrFI CCNGG 2 cut(s) 255, 353
SduI GDGCHC 1 cut(s) 192
SfcI CTRYAG 2 cut(s) 331, 440
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SsiI CCGC 1 cut(s) 466
SspMI CTAG 1 cut(s) 282
StyD4I CCNGG 2 cut(s) 253, 351
TaaI ACNGT 2 cut(s) 97, 368
TaqI TCGA 1 cut(s) 186
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 2 cut(s) 18, 267
Tru9I TTAA 2 cut(s) 18, 267
TscAI CASTG 1 cut(s) 100
TseI GCWGC 2 cut(s) 103, 248
TspDTI ATGAA 2 cut(s) 472, 481
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 1 cut(s) 100
Vha464I CTTAAG 1 cut(s) 266
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 1 cut(s) 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.