FvH4_2g31320
MYB Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0024041
Physical Location & Seq
Reverse (-)
24033454 .. 24040981
7528 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g31320.t1

Sequence Viewer

Length: 1431 bp
ATGAGGGGCTTTGCTTTTAAGCACCTCTGTTTTCAGTCCAAAAGCCAAAGCCAGCTATCAGGTGCTTCTGAAAGCCTGAAGCGCTTCCAAAGCACTGTGGCGCTGCTCTCTTTCCGTTCTCTCTTCTCCCTCTGCCCTTCGTTTTCGCTCACCACTTTCAGTCCTTCAGGGAAGCTTGTGCAGATCGAGCACGCTTTGACGGCAGTCGGGTCGGGTCAGACCTCTTTGGGTATCAAAGGTAATGTTGCTGCTCCGGGTTTTTACCTTAAGCTTGTGAGAGCTAGAGTTTATAGGCTTTGTGATTTGATGGGTGAGCTGGTGTTTAGCCTCTGCAGTCCTCCCCTAAATGCCCAGATGGCAAAAGCGTCGGCTGTTTGGAGCGAACAATTAGTACAAATATTTTGTGACTTGTGTATGAAGGAGGTGGACAATAATAACCGTCCAAACACTCATCTTAGTAAAGAGGGATATGAGAATGTGATACGTAATTTTGAAAAAGAAACGGGTAAACTATACACCAAAAAACAAATGAAAAACAAGTGGGATAACCTTAAAGAGCAGTGGAAACTATGGAAAGACTTGAAGGGTAAACAGACCGGTCTTGGTTGGGATCACAAGCTCCAGACTATAGATGCATCCGAAGATTGGTGGCGTGAGAAAATCAAGGAGAACAAGGAGTATGCTAAGTTACAGAAAAAAGGAATTACACCTGAGTTTGAAGACAAGTTGGATAAGTTATTTTCAGGAGTTGTAGCCACCGGTAGGTATGCTTGTACACCAGGTTCTTCACTACCTCAACCAACAAGTCCGGTTCACTCTGACAATAAACAAGACCTTGAAGAAAGTGGTGATTCATACGATATTCAGCCTACATCTACCCAAGGTATAAGAAAAAGAAGTGAGAGAATTGAAAAAGGTAAGGGAGTGGCTGCAAAAAAGGGGAAGGTGGGAGGTGCTGCTCTTCTGGCTACCAAAATTGATAGAATGTGTGAGGCAATTGAGAGCAGAAGTACCGCAACTTCAATTGCTAAGGGAGGCGCTAGCATCAAAGATGTGATGAAGGATGTTACTTCGTTGCCGGGTGGCGAGCCTTGTTCTCCATTGTGGTTCTTTGCCACGGATTTGTTTGTGTGCCAAGAGAAAAGAGAGATGTTTAGCACTATGGAAGATACTAACATGAGGTCGGAAGCAGAGCGTTTTCAACATTCCACAGAAACTATTAGTAGGTATTTTGGAAAGGCACTAGATATTTTGTATCAATATAGTGAAGAAATTATCAAACCAACAGATCCTCATTTTAATGGCATTGCCCCGGAGATCATGAATGATAGGAGATACATGCCACATTTTAAGGCTCCAAAGAAGGGCTCTAGGGGCCTTGAGGGCCAAATTTGGACATCTCCACTCACCTCCATCCCTCTAGCTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

477

Amino Acids

53.35

Weight (kDa)

9.1

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasome_A_N PF10584 48 - 68 3.2e-07 Proteasome subunit A N-terminal signature
Myb_DNA-bind_3 PF12776 126 - 219 8e-21 Myb/SANT-like DNA-binding domain
DUF8040 PF26138 388 - 418 9.2e-06 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024041)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22411 FvH4_2g31320 FvH4_7g08971

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1014
AclWI GGATC 2 cut(s) 618, 1283
AcsI RAATTY 1 cut(s) 1389
AcuI CTGAAG 2 cut(s) 98, 150
AfaI GTAC 3 cut(s) 393, 775, 1012
AfeI AGCGCT 1 cut(s) 83
AfiI CCNNNNNNNGG 3 cut(s) 645, 762, 1364
AflII CTTAAG 1 cut(s) 266
AgeI ACCGGT 2 cut(s) 596, 758
AgsI TTSAA 7 cut(s) 494, 583, 719, 839, 911, 1023, 1202
AjnI CCWGG 1 cut(s) 778
AluBI AGCT 7 cut(s) 55, 175, 271, 281, 316, 619, 1424
AluI AGCT 7 cut(s) 55, 175, 271, 281, 316, 619, 1424
Alw21I GWGCWC 1 cut(s) 192
AlwI GGATC 2 cut(s) 618, 1283
Aor51HI AGCGCT 1 cut(s) 83
AoxI GGCC 2 cut(s) 1375, 1384
ApeKI GCWGC 4 cut(s) 103, 248, 929, 956
ApoI RAATTY 1 cut(s) 1389
AsiGI ACCGGT 2 cut(s) 596, 758
Asp700I GAANNNNTTC 1 cut(s) 83
AspLEI GCGC 3 cut(s) 84, 103, 1040
AspS9I GGNCC 2 cut(s) 1375, 1384
AsuC2I CCSGG 3 cut(s) 255, 1080, 1313
AsuHPI GGTGA 4 cut(s) 142, 323, 860, 1399
AsuNHI GCTAGC 1 cut(s) 1040
BanII GRGCYC 1 cut(s) 1370
BarI GAAGNNNNNNTAC 2 cut(s) 1003, 1035
BbsI GAAGAC 1 cut(s) 726
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 4 cut(s) 90, 235, 916, 943
BccI CCATC 3 cut(s) 301, 349, 1421
BceAI ACGGC 1 cut(s) 216
BcgI CGANNNNNNTGC 4 cut(s) 192, 226, 348, 382
BciT130I CCWGG 1 cut(s) 780
BcnI CCSGG 3 cut(s) 255, 1080, 1313
BfaI CTAG 6 cut(s) 282, 1041, 1244, 1371, 1421, 1429
BfmI CTRYAG 2 cut(s) 331, 627
BfoI RGCGCY 3 cut(s) 85, 104, 1041
BfrI CTTAAG 1 cut(s) 266
BglI GCCNNNNNGGC 2 cut(s) 356, 1383
BisI GCNGC 4 cut(s) 104, 249, 930, 957
BlsI GCNGC 4 cut(s) 105, 250, 931, 958
Bme1390I CCNGG 4 cut(s) 255, 780, 1080, 1313
BmgT120I GGNCC 2 cut(s) 1375, 1384
BmiI GGNNCC 2 cut(s) 1356, 1376
BmrFI CCNGG 4 cut(s) 255, 780, 1080, 1313
BmsI GCATC 3 cut(s) 622, 644, 1053
BmtI GCTAGC 1 cut(s) 1044
BoxI GACNNNNGTC 1 cut(s) 203
BpiI GAAGAC 1 cut(s) 726
BpmI CTGGAG 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 1029
BpuEI CTTGAG 1 cut(s) 1400
BpuMI CCSGG 3 cut(s) 255, 1080, 1313
BsaAI YACGTR 1 cut(s) 485
BsaJI CCNNGG 3 cut(s) 880, 1116, 1311
BsaWI WCCGGW 3 cut(s) 596, 758, 808
Bsc4I CCNNNNNNNGG 3 cut(s) 645, 762, 1364
Bse118I RCCGGY 2 cut(s) 596, 758
Bse3DI GCAATG 1 cut(s) 1305
BseBI CCWGG 1 cut(s) 780
BseDI CCNNGG 3 cut(s) 880, 1116, 1311
BseGI GGATG 3 cut(s) 635, 1069, 1413
BseLI CCNNNNNNNGG 3 cut(s) 645, 762, 1364
BseMI GCAATG 1 cut(s) 1305
BseMII CTCAG 1 cut(s) 702
BseXI GCAGC 4 cut(s) 90, 235, 916, 943
BsgI GTGCAG 1 cut(s) 200
BshFI GGCC 2 cut(s) 1377, 1386
BshTI ACCGGT 2 cut(s) 596, 758
BsiHKAI GWGCWC 1 cut(s) 192
BsiSI CCGG 6 cut(s) 254, 597, 759, 809, 1079, 1313
BslI CCNNNNNNNGG 3 cut(s) 645, 762, 1364
BsnI GGCC 2 cut(s) 1377, 1386
Bsp1286I GDGCHC 2 cut(s) 192, 1370
Bsp1407I TGTACA 1 cut(s) 773
Bsp143I GATC 4 cut(s) 183, 610, 1288, 1317
BspACI CCGC 1 cut(s) 1014
BspANI GGCC 2 cut(s) 1377, 1386
BspCNI CTCAG 1 cut(s) 703
BspHI TCATGA 1 cut(s) 1320
BspLI GGNNCC 2 cut(s) 1356, 1376
BspMAI CTGCAG 1 cut(s) 335
BspOI GCTAGC 1 cut(s) 1044
BspPI GGATC 2 cut(s) 618, 1283
BspQI GCTCTTC 1 cut(s) 966
BspTI CTTAAG 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 1305
BsrFI RCCGGY 2 cut(s) 596, 758
BsrGI TGTACA 1 cut(s) 773
BssAI RCCGGY 2 cut(s) 596, 758
BssECI CCNNGG 3 cut(s) 880, 1116, 1311
BssMI GATC 4 cut(s) 183, 610, 1288, 1317
BssT1I CCWWGG 1 cut(s) 880
Bst2UI CCWGG 1 cut(s) 780
Bst4CI ACNGT 2 cut(s) 97, 440
Bst6I CTCTTC 2 cut(s) 128, 966
BstAFI CTTAAG 1 cut(s) 266
BstAUI TGTACA 1 cut(s) 773
BstBAI YACGTR 1 cut(s) 485
BstC8I GCNNGC 4 cut(s) 53, 192, 1042, 1088
BstDEI CTNAG 4 cut(s) 455, 684, 711, 1029
BstDSI CCRYGG 1 cut(s) 1116
BstF5I GGATG 3 cut(s) 635, 1069, 1413
BstH2I RGCGCY 3 cut(s) 85, 104, 1041
BstHHI GCGC 3 cut(s) 84, 103, 1040
BstKTI GATC 4 cut(s) 186, 613, 1291, 1320
BstMBI GATC 4 cut(s) 183, 610, 1288, 1317
BstMWI GCNNNNNNNGC 8 cut(s) 81, 90, 187, 200, 356, 965, 1374, 1383
BstNI CCWGG 1 cut(s) 780
BstNSI RCATGY 1 cut(s) 1342
BstPAI GACNNNNGTC 1 cut(s) 203
BstSCI CCNGG 4 cut(s) 253, 778, 1078, 1311
BstSFI CTRYAG 2 cut(s) 331, 627
BstSNI TACGTA 1 cut(s) 485
BstV1I GCAGC 4 cut(s) 90, 235, 916, 943
BstV2I GAAGAC 1 cut(s) 726
BstX2I RGATCY 1 cut(s) 1288
BstYI RGATCY 1 cut(s) 1288
BsuRI GGCC 2 cut(s) 1377, 1386
BtgI CCRYGG 1 cut(s) 1116
BtsCI GGATG 3 cut(s) 635, 1069, 1413
BtsI GCAGTG 1 cut(s) 566
BtsIMutI CAGTG 2 cut(s) 93, 566
Cac8I GCNNGC 4 cut(s) 53, 192, 1042, 1088
CciI TCATGA 1 cut(s) 1320
CfoI GCGC 3 cut(s) 84, 103, 1040
Cfr10I RCCGGY 2 cut(s) 596, 758
Cfr13I GGNCC 2 cut(s) 1375, 1384
CseI GACGC 1 cut(s) 354
CsiI ACCWGGT 1 cut(s) 778
Csp6I GTAC 3 cut(s) 392, 774, 1011
CspAI ACCGGT 2 cut(s) 596, 758
CviAII CATG 3 cut(s) 1177, 1321, 1339
CviQI GTAC 3 cut(s) 392, 774, 1011
DdeI CTNAG 4 cut(s) 455, 684, 711, 1029
DpnI GATC 4 cut(s) 185, 612, 1290, 1319
DpnII GATC 4 cut(s) 183, 610, 1288, 1317
Eam1104I CTCTTC 2 cut(s) 128, 966
EarI CTCTTC 2 cut(s) 128, 966
Eco105I TACGTA 1 cut(s) 485
Eco130I CCWWGG 1 cut(s) 880
Eco24I GRGCYC 1 cut(s) 1370
Eco47III AGCGCT 1 cut(s) 83
Eco57I CTGAAG 2 cut(s) 98, 150
EcoO109I RGGNCCY 1 cut(s) 1375
EcoRII CCWGG 1 cut(s) 778
EcoT14I CCWWGG 1 cut(s) 880
EcoT22I ATGCAT 1 cut(s) 637
EcoT38I GRGCYC 1 cut(s) 1370
ErhI CCWWGG 1 cut(s) 880
FaeI CATG 3 cut(s) 1180, 1324, 1342
FatI CATG 3 cut(s) 1176, 1320, 1338
Fnu4HI GCNGC 4 cut(s) 104, 249, 930, 957
FokI GGATG 3 cut(s) 622, 1076, 1400
FriOI GRGCYC 1 cut(s) 1370
Fsp4HI GCNGC 4 cut(s) 104, 249, 930, 957
FspBI CTAG 6 cut(s) 282, 1041, 1244, 1371, 1421, 1429
GlaI GCGC 3 cut(s) 83, 102, 1039
GluI GCNGC 4 cut(s) 104, 249, 930, 957
GsuI CTGGAG 1 cut(s) 605
HaeII RGCGCY 3 cut(s) 85, 104, 1041
HaeIII GGCC 2 cut(s) 1377, 1386
HapII CCGG 6 cut(s) 254, 597, 759, 809, 1079, 1313
HgaI GACGC 1 cut(s) 354
HhaI GCGC 3 cut(s) 84, 103, 1040
Hin1II CATG 3 cut(s) 1180, 1324, 1342
Hin6I GCGC 3 cut(s) 82, 101, 1038
HinP1I GCGC 3 cut(s) 82, 101, 1038
HindIII AAGCTT 2 cut(s) 173, 269
HinfI GANTC 1 cut(s) 851
HpaII CCGG 6 cut(s) 254, 597, 759, 809, 1079, 1313
HphI GGTGA 4 cut(s) 142, 323, 860, 1399
Hpy166II GTNNAC 5 cut(s) 427, 509, 590, 776, 814
Hpy188I TCNGA 5 cut(s) 70, 219, 640, 820, 1186
Hpy188III TCNNGA 3 cut(s) 622, 744, 1321
Hpy8I GTNNAC 5 cut(s) 427, 509, 590, 776, 814
Hpy99I CGWCG 1 cut(s) 370
HpyAV CCTTC 7 cut(s) 147, 174, 412, 577, 937, 1054, 1357
HpyCH4III ACNGT 2 cut(s) 97, 440
HpyCH4IV ACGT 1 cut(s) 484
HpyCH4V TGCA 4 cut(s) 181, 333, 635, 932
HpyF10VI GCNNNNNNNGC 8 cut(s) 81, 90, 187, 200, 356, 965, 1374, 1383
HpyF3I CTNAG 4 cut(s) 455, 684, 711, 1029
HpySE526I ACGT 1 cut(s) 484
Hsp92II CATG 3 cut(s) 1180, 1324, 1342
HspAI GCGC 3 cut(s) 82, 101, 1038
Kzo9I GATC 4 cut(s) 183, 610, 1288, 1317
LguI GCTCTTC 1 cut(s) 966
LmnI GCTCC 4 cut(s) 256, 378, 624, 1360
Lsp1109I GCAGC 4 cut(s) 90, 235, 916, 943
LweI GCATC 3 cut(s) 622, 644, 1053
MabI ACCWGGT 1 cut(s) 778
MaeI CTAG 6 cut(s) 282, 1041, 1244, 1371, 1421, 1429
MaeII ACGT 1 cut(s) 484
MaeIII GTNAC 3 cut(s) 404, 687, 1066
MalI GATC 4 cut(s) 185, 612, 1290, 1319
MboI GATC 4 cut(s) 183, 610, 1288, 1317
MboII GAAGA 8 cut(s) 115, 653, 731, 777, 851, 953, 1178, 1280
MfeI CAATTG 2 cut(s) 996, 1023
MflI RGATCY 1 cut(s) 1288
MhlI GDGCHC 2 cut(s) 192, 1370
MluCI AATT 9 cut(s) 386, 487, 702, 906, 975, 996, 1023, 1272, 1389
MmeI TCCRAC 2 cut(s) 708, 1164
Mph1103I ATGCAT 1 cut(s) 637
MroXI GAANNNNTTC 1 cut(s) 83
MseI TTAA 5 cut(s) 18, 267, 552, 1299, 1350
MslI CAYNNNNRTG 1 cut(s) 1299
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 6 cut(s) 254, 597, 759, 809, 1079, 1313
MspR9I CCNGG 4 cut(s) 255, 780, 1080, 1313
MunI CAATTG 2 cut(s) 996, 1023
MvaI CCWGG 1 cut(s) 780
MwoI GCNNNNNNNGC 8 cut(s) 81, 90, 187, 200, 356, 965, 1374, 1383
NciI CCSGG 3 cut(s) 255, 1080, 1313
NdeII GATC 4 cut(s) 183, 610, 1288, 1317
NheI GCTAGC 1 cut(s) 1040
NlaIII CATG 3 cut(s) 1180, 1324, 1342
NlaIV GGNNCC 2 cut(s) 1356, 1376
NmuCI GTSAC 1 cut(s) 404
NsiI ATGCAT 1 cut(s) 637
NspI RCATGY 1 cut(s) 1342
PagI TCATGA 1 cut(s) 1320
PciSI GCTCTTC 1 cut(s) 966
PdmI GAANNNNTTC 1 cut(s) 83
PfeI GAWTC 1 cut(s) 851
PinAI ACCGGT 2 cut(s) 596, 758
PkrI GCNGC 4 cut(s) 105, 250, 931, 958
Ppu21I YACGTR 1 cut(s) 485
PshAI GACNNNNGTC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 778
PspGI CCWGG 1 cut(s) 778
PspN4I GGNNCC 2 cut(s) 1356, 1376
PspPI GGNCC 2 cut(s) 1375, 1384
PsrI GAACNNNNNNTAC 4 cut(s) 375, 407, 766, 798
PstI CTGCAG 1 cut(s) 335
PsuI RGATCY 1 cut(s) 1288
RsaI GTAC 3 cut(s) 393, 775, 1012
RsaNI GTAC 3 cut(s) 392, 774, 1011
RseI CAYNNNNRTG 1 cut(s) 1299
SapI GCTCTTC 1 cut(s) 966
SaqAI TTAA 5 cut(s) 18, 267, 552, 1299, 1350
SatI GCNGC 4 cut(s) 104, 249, 930, 957
Sau3AI GATC 4 cut(s) 183, 610, 1288, 1317
Sau96I GGNCC 2 cut(s) 1375, 1384
ScrFI CCNGG 4 cut(s) 255, 780, 1080, 1313
SduI GDGCHC 2 cut(s) 192, 1370
SexAI ACCWGGT 1 cut(s) 778
SfaNI GCATC 3 cut(s) 622, 644, 1053
SfcI CTRYAG 2 cut(s) 331, 627
SfiI GGCCNNNNNGGCC 1 cut(s) 1383
SmiMI CAYNNNNRTG 1 cut(s) 1299
SmlI CTYRAG 2 cut(s) 266, 1379
SmoI CTYRAG 2 cut(s) 266, 1379
SnaBI TACGTA 1 cut(s) 485
Sse9I AATT 9 cut(s) 386, 487, 702, 906, 975, 996, 1023, 1272, 1389
SsiI CCGC 1 cut(s) 1014
SspI AATATT 1 cut(s) 399
SspMI CTAG 6 cut(s) 282, 1041, 1244, 1371, 1421, 1429
StyD4I CCNGG 4 cut(s) 253, 778, 1078, 1311
StyI CCWWGG 1 cut(s) 880
TaaI ACNGT 2 cut(s) 97, 440
TaiI ACGT 1 cut(s) 487
TaqI TCGA 1 cut(s) 186
TasI AATT 9 cut(s) 386, 487, 702, 906, 975, 996, 1023, 1272, 1389
TatI WGTACW 2 cut(s) 391, 773
TfiI GAWTC 1 cut(s) 851
Tru1I TTAA 5 cut(s) 18, 267, 552, 1299, 1350
Tru9I TTAA 5 cut(s) 18, 267, 552, 1299, 1350
TscAI CASTG 2 cut(s) 100, 566
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 4 cut(s) 103, 248, 929, 956
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 5 cut(s) 431, 545, 843, 1073, 1337
TspGWI ACGGA 2 cut(s) 104, 1133
TspRI CASTG 2 cut(s) 100, 566
Vha464I CTTAAG 1 cut(s) 266
XapI RAATTY 1 cut(s) 1389
XceI RCATGY 1 cut(s) 1342
XmnI GAANNNNTTC 1 cut(s) 83
XspI CTAG 6 cut(s) 282, 1041, 1244, 1371, 1421, 1429
Zsp2I ATGCAT 1 cut(s) 637
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.