FvH4_4g00110

Splicing factor 3B subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0027870
Physical Location & Seq
Reverse (-)
133959 .. 134330
372 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00110.t1

Sequence Viewer

Length: 372 bp
ATGTCAGACCCAGAGATAGCCAAGACCCAGGAGGAGCGGAAGAGGATGGAGCAGCAATTGGCCTCCATGAACTTCGTCACCTACGACGCCGAGTTCTACGGCGGCACCGACAAGGCCGACTACGTCTCCTCCATCCCCGTCAACGACGAGGAAGACAACCTCGACCCCGTCGAGAACGACGTCGTACGGAGGATGGCGTCGTACACGGCCCCCAAGTCGCTGATGAACGACATGCCCAGAGGCGGTGAGGATGACGAGGCCTCGGGGATGCTCCGGTCGAAGAAGATCATCGACCGCGAGGATGCTTACCGGCGCCGGAGGCTGAACCGGATCATATCGCCGGAGCGGCACGACCCGTTTGCGGCGGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
SF3b1

Protein Analysis

124

Amino Acids

14.12

Weight (kDa)

4.78

Isoelectric Point (pI)

72.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0027870)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00110
pyrus_communis pycom01g06170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 183
AccB1I GGYRCC 2 cut(s) 104, 312
AccBSI CCGCTC 2 cut(s) 37, 346
AccII CGCG 1 cut(s) 297
AciI CCGC 7 cut(s) 37, 102, 243, 295, 346, 362, 365
AclWI GGATC 1 cut(s) 338
AcyI GRCGYC 4 cut(s) 87, 180, 197, 313
AfaI GTAC 2 cut(s) 186, 203
AfiI CCNNNNNNNGG 2 cut(s) 242, 361
AjnI CCWGG 1 cut(s) 27
Alw26I GTCTC 1 cut(s) 130
AlwI GGATC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 262
AoxI GGCC 4 cut(s) 60, 114, 207, 258
ApeKI GCWGC 1 cut(s) 52
AspLEI GCGC 1 cut(s) 315
AspS9I GGNCC 1 cut(s) 208
AsuHPI GGTGA 2 cut(s) 70, 257
AvaI CYCGRG 1 cut(s) 262
BanI GGYRCC 2 cut(s) 104, 312
BbsI GAAGAC 1 cut(s) 159
BbvI GCAGC 1 cut(s) 64
BccI CCATC 3 cut(s) 40, 140, 187
BceAI ACGGC 2 cut(s) 115, 222
BcgI CGANNNNNNTGC 1 cut(s) 341
BciT130I CCWGG 1 cut(s) 29
BcoDI GTCTC 1 cut(s) 130
BfoI RGCGCY 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 346
BisI GCNGC 4 cut(s) 53, 103, 347, 363
BlsI GCNGC 4 cut(s) 54, 104, 348, 364
Bme1390I CCNGG 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 208
BmiI GGNNCC 3 cut(s) 106, 210, 314
BmrFI CCNGG 1 cut(s) 29
BmsI GCATC 2 cut(s) 258, 292
BpiI GAAGAC 1 cut(s) 159
BsaHI GRCGYC 4 cut(s) 87, 180, 197, 313
BsaJI CCNNGG 2 cut(s) 27, 261
BsaWI WCCGGW 2 cut(s) 273, 327
BsaXI ACNNNNNCTCC 4 cut(s) 110, 113, 140, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 361
Bse118I RCCGGY 1 cut(s) 309
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 2 cut(s) 27, 261
BseGI GGATG 6 cut(s) 51, 132, 198, 256, 273, 307
BseLI CCNNNNNNNGG 2 cut(s) 242, 361
BseRI GAGGAG 2 cut(s) 47, 118
BseXI GCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 297
Bsh1285I CGRYCG 2 cut(s) 278, 295
BshFI GGCC 4 cut(s) 62, 116, 209, 260
BshNI GGYRCC 2 cut(s) 104, 312
BsiEI CGRYCG 2 cut(s) 278, 295
BsiHKCI CYCGRG 1 cut(s) 262
BsiSI CCGG 5 cut(s) 274, 310, 316, 328, 341
BsiWI CGTACG 1 cut(s) 184
BslI CCNNNNNNNGG 2 cut(s) 242, 361
BsmAI GTCTC 1 cut(s) 130
BsmBI CGTCTC 1 cut(s) 130
BsnI GGCC 4 cut(s) 62, 116, 209, 260
BsoBI CYCGRG 1 cut(s) 262
Bsp143I GATC 2 cut(s) 285, 330
BspACI CCGC 7 cut(s) 37, 102, 243, 295, 346, 362, 365
BspANI GGCC 4 cut(s) 62, 116, 209, 260
BspFNI CGCG 1 cut(s) 297
BspLI GGNNCC 3 cut(s) 106, 210, 314
BspPI GGATC 1 cut(s) 338
BspT107I GGYRCC 2 cut(s) 104, 312
BsrBI CCGCTC 2 cut(s) 37, 346
BsrFI RCCGGY 1 cut(s) 309
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 27, 261
BssMI GATC 2 cut(s) 285, 330
BssNI GRCGYC 4 cut(s) 87, 180, 197, 313
Bst2UI CCWGG 1 cut(s) 29
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 4 cut(s) 87, 180, 197, 313
BstF5I GGATG 6 cut(s) 51, 132, 198, 256, 273, 307
BstFNI CGCG 1 cut(s) 297
BstH2I RGCGCY 1 cut(s) 316
BstHHI GCGC 1 cut(s) 315
BstKTI GATC 2 cut(s) 288, 333
BstMAI GTCTC 1 cut(s) 130
BstMBI GATC 2 cut(s) 285, 330
BstMCI CGRYCG 2 cut(s) 278, 295
BstMWI GCNNNNNNNGC 2 cut(s) 319, 346
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 235
BstSCI CCNGG 1 cut(s) 27
BstUI CGCG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 64
BstV2I GAAGAC 1 cut(s) 159
BsuRI GGCC 4 cut(s) 62, 116, 209, 260
BtsCI GGATG 6 cut(s) 51, 132, 198, 256, 273, 307
CfoI GCGC 1 cut(s) 315
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 208
CseI GACGC 2 cut(s) 95, 186
Csp6I GTAC 2 cut(s) 185, 202
CviAII CATG 2 cut(s) 67, 232
CviJI RGCY 6 cut(s) 20, 62, 116, 209, 260, 322
CviKI_1 RGCY 6 cut(s) 20, 62, 116, 209, 260, 322
CviQI GTAC 2 cut(s) 185, 202
DinI GGCGCC 1 cut(s) 314
DpnI GATC 2 cut(s) 287, 332
DpnII GATC 2 cut(s) 285, 330
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Eco147I AGGCCT 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 262
EcoRII CCWGG 1 cut(s) 27
EgeI GGCGCC 1 cut(s) 314
EheI GGCGCC 1 cut(s) 314
Esp3I CGTCTC 1 cut(s) 130
FaeI CATG 2 cut(s) 70, 235
FaiI YATR 3 cut(s) 68, 233, 335
FatI CATG 2 cut(s) 66, 231
FauI CCCGC 1 cut(s) 358
Fnu4HI GCNGC 4 cut(s) 53, 103, 347, 363
FokI GGATG 6 cut(s) 58, 119, 205, 263, 280, 314
Fsp4HI GCNGC 4 cut(s) 53, 103, 347, 363
GlaI GCGC 1 cut(s) 314
GluI GCNGC 4 cut(s) 53, 103, 347, 363
HaeII RGCGCY 1 cut(s) 316
HaeIII GGCC 4 cut(s) 62, 116, 209, 260
HapII CCGG 5 cut(s) 274, 310, 316, 328, 341
HgaI GACGC 2 cut(s) 95, 186
HhaI GCGC 1 cut(s) 315
Hin1I GRCGYC 4 cut(s) 87, 180, 197, 313
Hin1II CATG 2 cut(s) 70, 235
Hin6I GCGC 1 cut(s) 313
HinP1I GCGC 1 cut(s) 313
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HpaII CCGG 5 cut(s) 274, 310, 316, 328, 341
HphI GGTGA 2 cut(s) 70, 257
Hpy166II GTNNAC 2 cut(s) 142, 204
Hpy188I TCNGA 1 cut(s) 7
Hpy188III TCNNGA 1 cut(s) 172
Hpy8I GTNNAC 2 cut(s) 142, 204
Hpy99I CGWCG 6 cut(s) 89, 149, 173, 182, 185, 202
HpyCH4IV ACGT 2 cut(s) 123, 180
HpyF10VI GCNNNNNNNGC 2 cut(s) 319, 346
HpySE526I ACGT 2 cut(s) 123, 180
Hsp92I GRCGYC 4 cut(s) 87, 180, 197, 313
Hsp92II CATG 2 cut(s) 70, 235
HspAI GCGC 1 cut(s) 313
KasI GGCGCC 1 cut(s) 312
Kzo9I GATC 2 cut(s) 285, 330
LmnI GCTCC 4 cut(s) 34, 49, 276, 343
LpnPI CCDG 9 cut(s) 14, 24, 41, 250, 287, 323, 329, 341, 354
Lsp1109I GCAGC 1 cut(s) 64
LweI GCATC 2 cut(s) 258, 292
MaeII ACGT 2 cut(s) 123, 180
MaeIII GTNAC 1 cut(s) 76
MalI GATC 2 cut(s) 287, 332
MbiI CCGCTC 2 cut(s) 37, 346
MboI GATC 2 cut(s) 285, 330
MboII GAAGA 4 cut(s) 52, 164, 292, 295
MfeI CAATTG 1 cut(s) 56
MluCI AATT 1 cut(s) 56
Mly113I GGCGCC 1 cut(s) 313
MspI CCGG 5 cut(s) 274, 310, 316, 328, 341
MspR9I CCNGG 1 cut(s) 29
MunI CAATTG 1 cut(s) 56
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 1 cut(s) 297
MwoI GCNNNNNNNGC 2 cut(s) 319, 346
NarI GGCGCC 1 cut(s) 313
NdeII GATC 2 cut(s) 285, 330
NlaIII CATG 2 cut(s) 70, 235
NlaIV GGNNCC 3 cut(s) 106, 210, 314
NmeAIII GCCGAG 1 cut(s) 115
NmuCI GTSAC 1 cut(s) 76
NspI RCATGY 1 cut(s) 235
PceI AGGCCT 1 cut(s) 260
PcsI WCGNNNNNNNCGW 4 cut(s) 81, 105, 168, 177
Pfl23II CGTACG 1 cut(s) 184
PflFI GACNNNGTC 2 cut(s) 122, 167
PkrI GCNGC 4 cut(s) 54, 104, 348, 364
PluTI GGCGCC 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 27
PspGI CCWGG 1 cut(s) 27
PspLI CGTACG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 106, 210, 314
PspPI GGNCC 1 cut(s) 208
PsyI GACNNNGTC 2 cut(s) 122, 167
RsaI GTAC 2 cut(s) 186, 203
RsaNI GTAC 2 cut(s) 185, 202
SatI GCNGC 4 cut(s) 53, 103, 347, 363
Sau3AI GATC 2 cut(s) 285, 330
Sau96I GGNCC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 29
SetI ASST 4 cut(s) 83, 126, 162, 183
SfaNI GCATC 2 cut(s) 258, 292
SfoI GGCGCC 1 cut(s) 314
Sse9I AATT 1 cut(s) 56
SseBI AGGCCT 1 cut(s) 260
SsiI CCGC 7 cut(s) 37, 102, 243, 295, 346, 362, 365
SspDI GGCGCC 1 cut(s) 312
StuI AGGCCT 1 cut(s) 260
StyD4I CCNGG 1 cut(s) 27
TaiI ACGT 2 cut(s) 126, 183
TaqI TCGA 4 cut(s) 162, 171, 278, 291
TasI AATT 1 cut(s) 56
TauI GCSGC 3 cut(s) 105, 349, 365
TseFI GTSAC 1 cut(s) 76
TseI GCWGC 1 cut(s) 52
Tsp45I GTSAC 1 cut(s) 76
TspDTI ATGAA 2 cut(s) 83, 239
TspGWI ACGGA 1 cut(s) 202
Tth111I GACNNNGTC 2 cut(s) 122, 167
XceI RCATGY 1 cut(s) 235
ZraI GACGTC 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.