FvH4_4g10132

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0024015
Physical Location & Seq
Forward (+)
13051992 .. 13052694
703 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10132.t1

Sequence Viewer

Length: 621 bp
ATGGAACCTTGGTACTTACCAGAATCAGGTGAGGAGTCGGTGGCTGGCCAAGGTCGAGAGGACTTAGATTCAATGAGTAGAAGTGTCATTGACGATTTCAAAAATGTAAATCTGCCATCAGTGTTGCCGAGGCCTCATACTCATTGTTCTGACGCCGATCTAAATCCTTCGTCGCCGCTCAGCCCGGATCGAAGACCACAGAGCGACCAAGATGAGAGAGACTTTCACACGCTGTTTGATGAAGAAGAAGCTTGTACATTCCAGACGTCCCCCATCTGGAAATACAAAACCATAAAAGTACCAGCTGCCAATCCAAGTTTGACTTATAGTCATGTCAATAACCCGAAATCAGTAAAGCTAACACTGGAAAAGAGTGGATCTGCAAACCTAAAGTCTTATCAATATCTTCATCCGAAGCACTTGGGCATACTCCTGTCCATCTTCCTTGAAGACAAGCTCACACTTTTCGTCGTCAGCCTCGTTCGTACCTCTCCTCATGTTCTTTCCTCCCTTTCCCTTGTTCTTCGGCATGATTATTGGTTCTGCTCCTTTGTTTTAGCGAAAGTCGAAGGGAATTGGATGCAAGAGCAAAACATGAGAAATCAGCCCTTTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

207

Amino Acids

23.55

Weight (kDa)

5.73

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024015)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g00351 FvH4_4g10132
pyrus_communis pycom07g26270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 178
AciI CCGC 1 cut(s) 176
AclWI GGATC 2 cut(s) 195, 385
AcoI YGGCCR 1 cut(s) 46
AcyI GRCGYC 2 cut(s) 153, 266
AfaI GTAC 4 cut(s) 14, 256, 300, 487
AfiI CCNNNNNNNGG 2 cut(s) 26, 276
AgsI TTSAA 3 cut(s) 72, 100, 449
AhdI GACNNNNNGTC 1 cut(s) 327
AluBI AGCT 4 cut(s) 251, 305, 358, 457
AluI AGCT 4 cut(s) 251, 305, 358, 457
Alw26I GTCTC 1 cut(s) 213
AlwI GGATC 2 cut(s) 195, 385
AoxI GGCC 2 cut(s) 46, 131
ApeKI GCWGC 1 cut(s) 305
ArsI GACNNNNNNTTYG 2 cut(s) 377, 409
AsuC2I CCSGG 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 41
BalI TGGCCA 1 cut(s) 48
BbsI GAAGAC 2 cut(s) 199, 456
BbvI GCAGC 1 cut(s) 292
BccI CCATC 3 cut(s) 124, 281, 446
BcnI CCSGG 1 cut(s) 185
BcoDI GTCTC 1 cut(s) 213
BisI GCNGC 2 cut(s) 176, 306
BlpI GCTNAGC 1 cut(s) 179
BlsI GCNGC 2 cut(s) 177, 307
Bme1390I CCNGG 1 cut(s) 185
BmeRI GACNNNNNGTC 1 cut(s) 327
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 570
BpiI GAAGAC 2 cut(s) 199, 456
Bpu1102I GCTNAGC 1 cut(s) 179
BpuMI CCSGG 1 cut(s) 185
BsaBI GATNNNNATC 1 cut(s) 162
BsaHI GRCGYC 2 cut(s) 153, 266
BsaJI CCNNGG 3 cut(s) 8, 49, 128
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 276
Bse1I ACTGG 1 cut(s) 369
Bse8I GATNNNNATC 1 cut(s) 162
BseDI CCNNGG 3 cut(s) 8, 49, 128
BseGI GGATG 2 cut(s) 409, 585
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 2 cut(s) 26, 276
BseMII CTCAG 1 cut(s) 193
BseNI ACTGG 1 cut(s) 369
BseRI GAGGAG 2 cut(s) 47, 483
BseXI GCAGC 1 cut(s) 292
BshFI GGCC 2 cut(s) 48, 133
BsiSI CCGG 1 cut(s) 185
BslFI GGGAC 1 cut(s) 253
BslI CCNNNNNNNGG 2 cut(s) 26, 276
BsmAI GTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 253
BsnI GGCC 2 cut(s) 48, 133
Bsp1407I TGTACA 1 cut(s) 254
Bsp143I GATC 3 cut(s) 157, 187, 377
Bsp1720I GCTNAGC 1 cut(s) 179
BspACI CCGC 1 cut(s) 176
BspANI GGCC 2 cut(s) 48, 133
BspCNI CTCAG 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 195, 385
BsrBI CCGCTC 1 cut(s) 178
BsrGI TGTACA 1 cut(s) 254
BsrI ACTGG 1 cut(s) 369
BssECI CCNNGG 3 cut(s) 8, 49, 128
BssMI GATC 3 cut(s) 157, 187, 377
BssNI GRCGYC 2 cut(s) 153, 266
BssT1I CCWWGG 2 cut(s) 8, 49
BstACI GRCGYC 2 cut(s) 153, 266
BstAUI TGTACA 1 cut(s) 254
BstC8I GCNNGC 1 cut(s) 46
BstDEI CTNAG 2 cut(s) 64, 179
BstF5I GGATG 2 cut(s) 409, 585
BstKTI GATC 3 cut(s) 160, 190, 380
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 3 cut(s) 157, 187, 377
BstSCI CCNGG 1 cut(s) 183
BstV1I GCAGC 1 cut(s) 292
BstV2I GAAGAC 2 cut(s) 199, 456
BstX2I RGATCY 1 cut(s) 377
BstYI RGATCY 1 cut(s) 377
BsuRI GGCC 2 cut(s) 48, 133
BtsCI GGATG 2 cut(s) 409, 585
BtsIMutI CAGTG 2 cut(s) 126, 362
Cac8I GCNNGC 1 cut(s) 46
CseI GACGC 1 cut(s) 161
Csp6I GTAC 4 cut(s) 13, 255, 299, 486
CviAII CATG 4 cut(s) 332, 497, 530, 595
CviQI GTAC 4 cut(s) 13, 255, 299, 486
DdeI CTNAG 2 cut(s) 64, 179
DpnI GATC 3 cut(s) 159, 189, 379
DpnII GATC 3 cut(s) 157, 187, 377
DriI GACNNNNNGTC 1 cut(s) 327
EaeI YGGCCR 1 cut(s) 46
Eam1105I GACNNNNNGTC 1 cut(s) 327
Eco130I CCWWGG 2 cut(s) 8, 49
Eco147I AGGCCT 1 cut(s) 133
EcoT14I CCWWGG 2 cut(s) 8, 49
ErhI CCWWGG 2 cut(s) 8, 49
FaeI CATG 4 cut(s) 335, 500, 533, 598
FaiI YATR 8 cut(s) 138, 293, 327, 333, 428, 498, 531, 596
FalI AAGNNNNNCTT 2 cut(s) 307, 339
FaqI GGGAC 1 cut(s) 253
FatI CATG 4 cut(s) 331, 496, 529, 594
Fnu4HI GCNGC 2 cut(s) 176, 306
FokI GGATG 2 cut(s) 396, 592
Fsp4HI GCNGC 2 cut(s) 176, 306
GluI GCNGC 2 cut(s) 176, 306
HaeIII GGCC 2 cut(s) 48, 133
HapII CCGG 1 cut(s) 185
HgaI GACGC 1 cut(s) 161
Hin1I GRCGYC 2 cut(s) 153, 266
Hin1II CATG 4 cut(s) 335, 500, 533, 598
HindIII AAGCTT 1 cut(s) 249
HinfI GANTC 3 cut(s) 23, 35, 68
HpaII CCGG 1 cut(s) 185
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 2 cut(s) 151, 414
Hpy188III TCNNGA 3 cut(s) 56, 262, 277
Hpy99I CGWCG 2 cut(s) 175, 473
HpyAV CCTTC 2 cut(s) 177, 563
HpyCH4IV ACGT 1 cut(s) 266
HpyCH4V TGCA 2 cut(s) 383, 583
HpyF3I CTNAG 2 cut(s) 64, 179
HpySE526I ACGT 1 cut(s) 266
Hsp92I GRCGYC 2 cut(s) 153, 266
Hsp92II CATG 4 cut(s) 335, 500, 533, 598
Kzo9I GATC 3 cut(s) 157, 187, 377
LmnI GCTCC 1 cut(s) 551
LpnPI CCDG 9 cut(s) 12, 30, 33, 198, 262, 275, 315, 350, 446
Lsp1109I GCAGC 1 cut(s) 292
LweI GCATC 1 cut(s) 570
MaeII ACGT 1 cut(s) 266
MalI GATC 3 cut(s) 159, 189, 379
MbiI CCGCTC 1 cut(s) 178
MboI GATC 3 cut(s) 157, 187, 377
MboII GAAGA 7 cut(s) 204, 254, 257, 398, 433, 461, 515
MflI RGATCY 1 cut(s) 377
MlsI TGGCCA 1 cut(s) 48
MluCI AATT 1 cut(s) 574
MluNI TGGCCA 1 cut(s) 48
MlyI GAGTC 1 cut(s) 44
MnlI CCTC 8 cut(s) 25, 52, 123, 144, 488, 499, 504, 517
Mox20I TGGCCA 1 cut(s) 48
MscI TGGCCA 1 cut(s) 48
Msp20I TGGCCA 1 cut(s) 48
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 1 cut(s) 185
MspR9I CCNGG 1 cut(s) 185
NciI CCSGG 1 cut(s) 185
NdeII GATC 3 cut(s) 157, 187, 377
NlaIII CATG 4 cut(s) 335, 500, 533, 598
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 153
PceI AGGCCT 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PfeI GAWTC 2 cut(s) 23, 68
PkrI GCNGC 2 cut(s) 177, 307
PleI GAGTC 1 cut(s) 43
PpsI GAGTC 1 cut(s) 43
PspN4I GGNNCC 1 cut(s) 6
PsuI RGATCY 1 cut(s) 377
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 4 cut(s) 14, 256, 300, 487
RsaNI GTAC 4 cut(s) 13, 255, 299, 486
SatI GCNGC 2 cut(s) 176, 306
Sau3AI GATC 3 cut(s) 157, 187, 377
SchI GAGTC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 185
SfaNI GCATC 1 cut(s) 570
Sse9I AATT 1 cut(s) 574
SseBI AGGCCT 1 cut(s) 133
SsiI CCGC 1 cut(s) 176
StuI AGGCCT 1 cut(s) 133
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 2 cut(s) 8, 49
TaiI ACGT 1 cut(s) 269
TaqI TCGA 3 cut(s) 55, 190, 567
TasI AATT 1 cut(s) 574
TatI WGTACW 1 cut(s) 254
TauI GCSGC 1 cut(s) 178
TfiI GAWTC 2 cut(s) 23, 68
TscAI CASTG 2 cut(s) 126, 369
TseI GCWGC 1 cut(s) 305
TspDTI ATGAA 2 cut(s) 255, 398
TspRI CASTG 2 cut(s) 126, 369
ZraI GACGTC 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.