FvH4_4g15294

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0024013
Physical Location & Seq
Reverse (-)
18928204 .. 18929809
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15294.t1

Sequence Viewer

Length: 1254 bp
ATGGGTGACGAAAGAACAGACAAAGAGAGACTTGATGCGGTAGAGAAGCAGTTGGCGTCGATAAGTTCTACTCTTGCGCTACTGGCCAATTTCATCAAACAGGAGAAGAAAGTCGAACCTTTGGAGAAGCCGAAGAACAAGACTTCTGATTCAGATGACGAAAGGGATGAAGCAGATTCGGATGAGGAAACAGACGACACCAGCAAAACTTTGCATCAAGGAGGCAAGGGTTCTTTTCGGAATCTGAAGATCGATTTCAAAGTTGAAATCCTTCTGTATGACGGGAGCGTCAACGTAGAGAAACTAGACGATTGGATTGAGTGTCTAGAGACTTATTTTACTCTTTATAACTACTCGTCAGAGGAGAAGATTATCTTTGCAACTCTAAAACTATCGGCTCATGCATTGACTTGGTGGAAGTCCTATCAGAAAAATAGTGACGGAGAGGCAATGTCGTGGAAGAAGTTCAAGGAGTTGCTTAGGAAGCAGTTCTATCCAATCGGTTTTCTGGAAGAAAGATGGTGCAAGTGGTACAACTTGCGTCAAGAATTCAATCAATCCGTACAAGAATACACCACCGAATTCCAGAATCAAGCTATGGTTTTGGATATTTCATTGAAGGAGTATTCTGTGTTCATGAAATATGTGGCGAGTCTGAGTGAGTATACCCAGAAGGAGTTGAAATTATTCACGGTGGAAACTATTGCAGAAGCCAGCGTGAAAGCCATTGCCATTGAGGGCAAATTTAAGAAGAATGTAACAGAAAGATATGGAAAACAATCTGTGGGTAAGTCCAATGGTTCCTCTGTCAAAAAGAAGGAGCATGAGGGTGGTGAGTCTGATGGGAAAGAATGTTTGGTTTGCAGTCACTGTGATGGAACAGGCCGTGTGGTGGATAAGTGTTGGGTGAAGTATCCTCACCTTAAACCAAGAGGGTTAAGGCAGAAGGAGGAGAAAAAGAAAGTCGCTTTGACCACACAGGAGCCAAAGGAAGTATCAGGGATGCCAAAACCAAATGCAAAGCTCAATCTTATGGCAGGAAATAAGCAACCTGTAACGGATGAAGCAGACCCCAAGGAGCAGCTCTTTGTTGTTAAGTTACAGGTGAAGATCAATTTGGTTGATGCCATTGTGGATCCGGGAGTCAGATTAATCTTATCTCGGAAGCCTTGGTTCAGAAGCTGGGTCTCAAGACAGTCAGACATCCGAAGCCATATCCGTTGGGATGGATCCAAAAGGAAGCAGGCGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

418

Amino Acids

48.01

Weight (kDa)

8.58

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 86 - 244 4.9e-13 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 126 - 216 4.8e-12 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024013)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29101 FvH4_4g15294
rosa_multiflora Rmu_sc0001374.1_g000055

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AasI GACNNNNNNGTC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 665
AciI CCGC 2 cut(s) 38, 1247
AclWI GGATC 4 cut(s) 1130, 1143, 1224, 1237
AcoI YGGCCR 1 cut(s) 84
AcsI RAATTY 3 cut(s) 548, 581, 743
AcuI CTGAAG 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 2 cut(s) 533, 564
AfiI CCNNNNNNNGG 1 cut(s) 892
AgsI TTSAA 6 cut(s) 259, 266, 469, 553, 619, 682
AjuI GAANNNNNNNTTGG 4 cut(s) 839, 871, 1100, 1132
AluBI AGCT 4 cut(s) 596, 1024, 1084, 1182
AluI AGCT 4 cut(s) 596, 1024, 1084, 1182
Alw26I GTCTC 3 cut(s) 22, 323, 1192
AlwI GGATC 4 cut(s) 1130, 1143, 1224, 1237
AlwNI CAGNNNCTG 2 cut(s) 870, 1182
AoxI GGCC 2 cut(s) 84, 883
ApeKI GCWGC 1 cut(s) 1081
ApoI RAATTY 3 cut(s) 548, 581, 743
AseI ATTAAT 1 cut(s) 1151
Asp700I GAANNNNTTC 4 cut(s) 270, 464, 488, 686
AspLEI GCGC 1 cut(s) 79
AsuC2I CCSGG 1 cut(s) 1140
AsuHPI GGTGA 5 cut(s) 17, 845, 911, 919, 1117
BalI TGGCCA 1 cut(s) 86
BamHI GGATCC 2 cut(s) 1135, 1229
BbvI GCAGC 1 cut(s) 1093
BccI CCATC 4 cut(s) 513, 836, 869, 1220
BceAI ACGGC 1 cut(s) 870
BciVI GTATCC 1 cut(s) 924
BcnI CCSGG 1 cut(s) 1140
BcoDI GTCTC 3 cut(s) 22, 323, 1192
BfaI CTAG 2 cut(s) 305, 326
BfuI GTATCC 1 cut(s) 924
BisI GCNGC 1 cut(s) 1082
BlsI GCNGC 1 cut(s) 1083
Bme1390I CCNGG 1 cut(s) 1140
BmiI GGNNCC 4 cut(s) 802, 984, 1137, 1231
BmrFI CCNGG 1 cut(s) 1140
BmsI GCATC 4 cut(s) 25, 223, 993, 1114
Bpu10I CCTNAGC 1 cut(s) 479
BpuEI CTTGAG 1 cut(s) 1174
BpuMI CCSGG 1 cut(s) 1140
Bsa29I ATCGAT 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 1 cut(s) 1192
BsaJI CCNNGG 2 cut(s) 1074, 1169
Bsc4I CCNNNNNNNGG 1 cut(s) 892
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 2 cut(s) 456, 726
BseCI ATCGAT 1 cut(s) 252
BseDI CCNNGG 2 cut(s) 1074, 1169
BseGI GGATG 6 cut(s) 172, 187, 1008, 1066, 1203, 1231
BseLI CCNNNNNNNGG 1 cut(s) 892
BseMI GCAATG 2 cut(s) 456, 726
BseMII CTCAG 1 cut(s) 647
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 2 cut(s) 377, 965
BseXI GCAGC 1 cut(s) 1093
BseYI CCCAGC 1 cut(s) 1182
BshFI GGCC 2 cut(s) 86, 885
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 1 cut(s) 1139
BslI CCNNNNNNNGG 1 cut(s) 892
BsmAI GTCTC 3 cut(s) 22, 323, 1192
BsnI GGCC 2 cut(s) 86, 885
Bso31I GGTCTC 1 cut(s) 1192
Bsp143I GATC 4 cut(s) 249, 1110, 1135, 1229
BspACI CCGC 2 cut(s) 38, 1247
BspANI GGCC 2 cut(s) 86, 885
BspCNI CTCAG 1 cut(s) 648
BspDI ATCGAT 1 cut(s) 252
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 4 cut(s) 802, 984, 1137, 1231
BspPI GGATC 4 cut(s) 1130, 1143, 1224, 1237
BspTNI GGTCTC 1 cut(s) 1192
BsrDI GCAATG 2 cut(s) 456, 726
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 2 cut(s) 1074, 1169
BssMI GATC 4 cut(s) 249, 1110, 1135, 1229
BssNAI GTATAC 1 cut(s) 666
BssNI GRCGYC 1 cut(s) 56
BssT1I CCWWGG 2 cut(s) 1074, 1169
Bst1107I GTATAC 1 cut(s) 666
Bst4CI ACNGT 3 cut(s) 694, 872, 1197
BstACI GRCGYC 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 715, 1245
BstDEI CTNAG 2 cut(s) 479, 656
BstF5I GGATG 6 cut(s) 172, 187, 1008, 1066, 1203, 1231
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 4 cut(s) 252, 1113, 1138, 1232
BstMAI GTCTC 3 cut(s) 22, 323, 1192
BstMBI GATC 4 cut(s) 249, 1110, 1135, 1229
BstMWI GCNNNNNNNGC 2 cut(s) 83, 484
BstSCI CCNGG 1 cut(s) 1138
BstV1I GCAGC 1 cut(s) 1093
BstX2I RGATCY 2 cut(s) 1135, 1229
BstYI RGATCY 2 cut(s) 1135, 1229
BstZ17I GTATAC 1 cut(s) 666
Bsu15I ATCGAT 1 cut(s) 252
BsuI GTATCC 1 cut(s) 924
BsuRI GGCC 2 cut(s) 86, 885
BsuTUI ATCGAT 1 cut(s) 252
BtsCI GGATG 6 cut(s) 172, 187, 1008, 1066, 1203, 1231
BtsIMutI CAGTG 1 cut(s) 868
Cac8I GCNNGC 2 cut(s) 715, 1245
CaiI CAGNNNCTG 2 cut(s) 870, 1182
CciI TCATGA 1 cut(s) 636
CfoI GCGC 1 cut(s) 79
ClaI ATCGAT 1 cut(s) 252
CseI GACGC 3 cut(s) 45, 277, 530
Csp6I GTAC 2 cut(s) 532, 563
CviAII CATG 3 cut(s) 401, 637, 824
CviQI GTAC 2 cut(s) 532, 563
DdeI CTNAG 2 cut(s) 479, 656
DpnI GATC 4 cut(s) 251, 1112, 1137, 1231
DpnII GATC 4 cut(s) 249, 1110, 1135, 1229
DrdI GACNNNNNNGTC 1 cut(s) 287
DseDI GACNNNNNNGTC 1 cut(s) 287
EaeI YGGCCR 1 cut(s) 84
Eco130I CCWWGG 2 cut(s) 1074, 1169
Eco31I GGTCTC 1 cut(s) 1192
Eco57I CTGAAG 1 cut(s) 266
EcoRI GAATTC 2 cut(s) 548, 581
EcoT14I CCWWGG 2 cut(s) 1074, 1169
EcoT22I ATGCAT 1 cut(s) 406
ErhI CCWWGG 2 cut(s) 1074, 1169
FaeI CATG 3 cut(s) 404, 640, 827
FalI AAGNNNNNCTT 4 cut(s) 15, 47, 359, 391
FatI CATG 3 cut(s) 400, 636, 823
FauI CCCGC 1 cut(s) 1240
FblI GTMKAC 1 cut(s) 665
Fnu4HI GCNGC 1 cut(s) 1082
FokI GGATG 6 cut(s) 179, 194, 1015, 1073, 1190, 1238
Fsp4HI GCNGC 1 cut(s) 1082
FspBI CTAG 2 cut(s) 305, 326
GlaI GCGC 1 cut(s) 78
GluI GCNGC 1 cut(s) 1082
GsaI CCCAGC 1 cut(s) 1186
HaeIII GGCC 2 cut(s) 86, 885
HapII CCGG 1 cut(s) 1139
HgaI GACGC 3 cut(s) 45, 277, 530
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 56
Hin1II CATG 3 cut(s) 404, 640, 827
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 7 cut(s) 149, 176, 241, 589, 652, 836, 1143
HpaII CCGG 1 cut(s) 1139
HphI GGTGA 5 cut(s) 17, 845, 911, 919, 1117
Hpy166II GTNNAC 2 cut(s) 292, 666
Hpy188III TCNNGA 6 cut(s) 326, 509, 545, 586, 637, 1191
Hpy8I GTNNAC 2 cut(s) 292, 666
Hpy99I CGWCG 1 cut(s) 61
HpyAV CCTTC 5 cut(s) 281, 613, 667, 811, 940
HpyCH4III ACNGT 3 cut(s) 694, 872, 1197
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 7 cut(s) 214, 380, 404, 525, 707, 864, 1019
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 484
HpyF3I CTNAG 2 cut(s) 479, 656
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 56
Hsp92II CATG 3 cut(s) 404, 640, 827
HspAI GCGC 1 cut(s) 77
Kzo9I GATC 4 cut(s) 249, 1110, 1135, 1229
LmnI GCTCC 4 cut(s) 285, 820, 982, 1078
Lsp1109I GCAGC 1 cut(s) 1093
LweI GCATC 4 cut(s) 25, 223, 993, 1114
MaeI CTAG 2 cut(s) 305, 326
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 6 cut(s) 5, 437, 757, 866, 1054, 1098
MalI GATC 4 cut(s) 251, 1112, 1137, 1231
MboI GATC 4 cut(s) 249, 1110, 1135, 1229
MboII GAAGA 8 cut(s) 118, 145, 259, 379, 472, 524, 763, 1120
MflI RGATCY 2 cut(s) 1135, 1229
MlsI TGGCCA 1 cut(s) 86
MluCI AATT 6 cut(s) 88, 548, 581, 683, 743, 1114
MluNI TGGCCA 1 cut(s) 86
MlyI GAGTC 3 cut(s) 661, 845, 1152
Mox20I TGGCCA 1 cut(s) 86
Mph1103I ATGCAT 1 cut(s) 406
MroXI GAANNNNTTC 4 cut(s) 270, 464, 488, 686
MscI TGGCCA 1 cut(s) 86
MseI TTAA 5 cut(s) 747, 924, 938, 1095, 1151
MslI CAYNNNNRTG 2 cut(s) 828, 873
Msp20I TGGCCA 1 cut(s) 86
MspI CCGG 1 cut(s) 1139
MspR9I CCNGG 1 cut(s) 1140
MwoI GCNNNNNNNGC 2 cut(s) 83, 484
NciI CCSGG 1 cut(s) 1140
NdeII GATC 4 cut(s) 249, 1110, 1135, 1229
NlaIII CATG 3 cut(s) 404, 640, 827
NlaIV GGNNCC 4 cut(s) 802, 984, 1137, 1231
NmuCI GTSAC 3 cut(s) 5, 437, 866
NsiI ATGCAT 1 cut(s) 406
PagI TCATGA 1 cut(s) 636
PdmI GAANNNNTTC 4 cut(s) 270, 464, 488, 686
PfeI GAWTC 4 cut(s) 149, 176, 241, 589
PfoI TCCNGGA 1 cut(s) 1138
PkrI GCNGC 1 cut(s) 1083
PleI GAGTC 3 cut(s) 660, 844, 1151
PpsI GAGTC 3 cut(s) 660, 844, 1151
PshBI ATTAAT 1 cut(s) 1151
PsiI TTATAA 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 1182
PspN4I GGNNCC 4 cut(s) 802, 984, 1137, 1231
PsrI GAACNNNNNNTAC 2 cut(s) 617, 649
PstNI CAGNNNCTG 2 cut(s) 870, 1182
PsuI RGATCY 2 cut(s) 1135, 1229
RsaI GTAC 2 cut(s) 533, 564
RsaNI GTAC 2 cut(s) 532, 563
RseI CAYNNNNRTG 2 cut(s) 828, 873
SaqAI TTAA 5 cut(s) 747, 924, 938, 1095, 1151
SatI GCNGC 1 cut(s) 1082
Sau3AI GATC 4 cut(s) 249, 1110, 1135, 1229
SchI GAGTC 3 cut(s) 661, 845, 1152
ScrFI CCNGG 1 cut(s) 1140
SetI ASST 9 cut(s) 121, 297, 598, 924, 1026, 1054, 1086, 1107, 1184
SfaNI GCATC 4 cut(s) 25, 223, 993, 1114
SmiMI CAYNNNNRTG 2 cut(s) 828, 873
SmlI CTYRAG 1 cut(s) 1189
SmoI CTYRAG 1 cut(s) 1189
Sse9I AATT 6 cut(s) 88, 548, 581, 683, 743, 1114
SsiI CCGC 2 cut(s) 38, 1247
SspMI CTAG 2 cut(s) 305, 326
StyD4I CCNGG 1 cut(s) 1138
StyI CCWWGG 2 cut(s) 1074, 1169
TaaI ACNGT 3 cut(s) 694, 872, 1197
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 59, 114, 252
TasI AATT 6 cut(s) 88, 548, 581, 683, 743, 1114
TfiI GAWTC 4 cut(s) 149, 176, 241, 589
Tru1I TTAA 5 cut(s) 747, 924, 938, 1095, 1151
Tru9I TTAA 5 cut(s) 747, 924, 938, 1095, 1151
TscAI CASTG 1 cut(s) 875
TseFI GTSAC 3 cut(s) 5, 437, 866
TseI GCWGC 1 cut(s) 1081
Tsp45I GTSAC 3 cut(s) 5, 437, 866
TspDTI ATGAA 6 cut(s) 82, 183, 603, 625, 653, 1077
TspGWI ACGGA 4 cut(s) 456, 550, 1073, 1208
TspRI CASTG 1 cut(s) 875
VspI ATTAAT 1 cut(s) 1151
XapI RAATTY 3 cut(s) 548, 581, 743
XbaI TCTAGA 1 cut(s) 325
XmiI GTMKAC 1 cut(s) 665
XmnI GAANNNNTTC 4 cut(s) 270, 464, 488, 686
XspI CTAG 2 cut(s) 305, 326
Zsp2I ATGCAT 1 cut(s) 406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.