FvH4_5g06641

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0019705
Physical Location & Seq
Reverse (-)
3930283 .. 3934318
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06641.t2

Sequence Viewer

Length: 1323 bp
ATGTCGGAACCAGAAGACAATCAAGGAACCCCGAGTAAGCAAAAAGCTGTGAGCAGTGAATATACTCACCAATCTGCTCATATCGTAGGCAATGATTCATGGCAACAAGTTGGGTTGATGCTGGTGACCAGCTTCAATTGTGGGTACATATTGAGCTTTTCATTTCTTCAGTTGGCGTGTTTGGGTTGGAAACGGGGTATCACATGCCTACTGTTTTTTGGGGTCTATACTTTTTATGCCCACTGGCTTATGGCTGGTTTTCACATCATCAATGGCCAAAGATTTATCAGATTCAGAGATCTCATGGGATATACCTGTGGAAGAAAGATGTATTACATCACGTTTTACCTCCAATACTTGGTCATTCTTCTTACAAACATGGGTTTCATTCTCCTTGGGGGAAACGCACTCAAGGCGATAAACATGGAATTCAGCGAGAATCCTTGGAGGGTCCAATACTATATCGTTCTCACTGGAGCAGCCTTCTTCATTTTCGCATTCTGCGTTCCTACAATGTCTCATATGGGAATCTGGCTCATCCCCTCTACAGTTATCACCTTTTTGTATATTATCACACTCTTGGTGGTAGTGATTAACGATGGGAAAAATAACACAAACCGTGATTATCGTGTCCCCGGAACCAAAGAAGAGAGGGTGTTCGGAGCCTTTAATGCAATTTCAGCAATTGTTGTGAGCAGTAGTTCAGGATTATTACCAGAAATACAGTCAACTGTTAAGAAGCCTGAAGTGGCAAATATGAGGAAGGCTCTCTACATGCAGTACACTGTGGGGATATTGTTTTACTATGGAGTTAGCATTGCAGGATACTGGGCATATGGCAATGATGTATCTTCATACCTCCCCGATATGCTAAGTGGTCCGAAATGGGTCAAGGTTCTCATAAACGCTGCTGTTTTTCTACAAGCCATAGTATCCCAACATACATTTGTTATGCCGATTCATGAGGCCCTAGATACCGGTTCCCTCCTACTGGATAAGAGCATTAACTCGCCAGAAAATCTCAAGAGAAGATTCTACCTTCGATTCCTCCTCTTTGCGGGAAATACATTTATGACAGCAGCTATTCCTTTCATGGCGGACTTTGTAGCCTTGTTTGGAGCATTAGCACTCATTCCTCTAACTCTTGTGTTCCCAAGTTTGGTTTTCATTAGAGTCAAGGGAAAGACAGCCAGATTAGGGGAGAACTTATGGCATTGGTTCAACATTATTGCGTTTTCTTCCCTGGCGCTTGTTGCCACTATTGCTGCAGTTTATGAGATAGTCAACAGTGTTGAATTATATAGTTTCTTTGCAGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

441

Amino Acids

49.55

Weight (kDa)

8.89

Isoelectric Point (pI)

38.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 31 - 425 1.2e-54 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06641 FvH4_5g06641
rosa_chinensis RchiOBHm_Chr7g0192041
rosa_laevigata RLG00000004406
rosa_rugosa Rorug06G0512600
rosa_samantha Rh7AG123000
rosa_wichuraiana Rw7G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 358
AciI CCGC 2 cut(s) 1058, 1097
AcoI YGGCCR 1 cut(s) 274
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 2 cut(s) 152, 765
AfaI GTAC 2 cut(s) 146, 782
AfiI CCNNNNNNNGG 5 cut(s) 358, 991, 1057, 1159, 1197
AgeI ACCGGT 1 cut(s) 977
AgsI TTSAA 3 cut(s) 136, 1222, 1295
AjnI CCWGG 1 cut(s) 1242
AluBI AGCT 4 cut(s) 47, 132, 156, 1082
AluI AGCT 4 cut(s) 47, 132, 156, 1082
Alw26I GTCTC 1 cut(s) 522
Ama87I CYCGRG 1 cut(s) 31
AoxI GGCC 2 cut(s) 274, 966
ApeKI GCWGC 4 cut(s) 479, 908, 1079, 1265
ApoI RAATTY 1 cut(s) 428
AsiGI ACCGGT 1 cut(s) 977
AspLEI GCGC 1 cut(s) 1249
AspS9I GGNCC 3 cut(s) 451, 878, 967
AsuC2I CCSGG 1 cut(s) 636
AsuHPI GGTGA 3 cut(s) 59, 136, 547
AvaI CYCGRG 1 cut(s) 31
AvaII GGWCC 2 cut(s) 451, 878
BalI TGGCCA 1 cut(s) 276
BarI GAAGNNNNNNTAC 2 cut(s) 755, 787
BbsI GAAGAC 1 cut(s) 21
BbvI GCAGC 4 cut(s) 491, 895, 1091, 1252
BccI CCATC 1 cut(s) 593
BciT130I CCWGG 1 cut(s) 1244
BciVI GTATCC 2 cut(s) 818, 943
BcnI CCSGG 1 cut(s) 636
BcoDI GTCTC 1 cut(s) 522
BfaI CTAG 1 cut(s) 971
BfmI CTRYAG 2 cut(s) 546, 1266
BfoI RGCGCY 1 cut(s) 1250
BfuI GTATCC 2 cut(s) 818, 943
BglII AGATCT 1 cut(s) 298
BisI GCNGC 4 cut(s) 480, 909, 1080, 1266
BlsI GCNGC 4 cut(s) 481, 910, 1081, 1267
Bme1390I CCNGG 2 cut(s) 636, 1244
Bme18I GGWCC 2 cut(s) 451, 878
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 3 cut(s) 451, 878, 967
BmiI GGNNCC 6 cut(s) 9, 28, 452, 640, 664, 982
BmrFI CCNGG 2 cut(s) 636, 1244
BmrI ACTGGG 1 cut(s) 838
BmsI GCATC 1 cut(s) 108
BmuI ACTGGG 1 cut(s) 838
BpiI GAAGAC 1 cut(s) 21
BplI GAGNNNNNCTC 2 cut(s) 751, 783
BpmI CTGGAG 1 cut(s) 495
BpuEI CTTGAG 2 cut(s) 395, 1007
BpuMI CCSGG 1 cut(s) 636
BsaJI CCNNGG 4 cut(s) 394, 443, 634, 1242
BsaWI WCCGGW 1 cut(s) 977
Bsc4I CCNNNNNNNGG 5 cut(s) 358, 991, 1057, 1159, 1197
Bse118I RCCGGY 1 cut(s) 977
Bse1I ACTGG 4 cut(s) 248, 478, 833, 996
Bse3DI GCAATG 3 cut(s) 97, 816, 847
BseBI CCWGG 1 cut(s) 1244
BseDI CCNNGG 4 cut(s) 394, 443, 634, 1242
BseGI GGATG 1 cut(s) 537
BseLI CCNNNNNNNGG 5 cut(s) 358, 991, 1057, 1159, 1197
BseMI GCAATG 3 cut(s) 97, 816, 847
BseNI ACTGG 4 cut(s) 248, 478, 833, 996
BseRI GAGGAG 1 cut(s) 1040
BseXI GCAGC 4 cut(s) 491, 895, 1091, 1252
BshFI GGCC 2 cut(s) 276, 968
BshTI ACCGGT 1 cut(s) 977
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 2 cut(s) 636, 978
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 5 cut(s) 358, 991, 1057, 1159, 1197
BsmAI GTCTC 1 cut(s) 522
BsmFI GGGAC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 497
BsnI GGCC 2 cut(s) 276, 968
BsoBI CYCGRG 1 cut(s) 31
Bsp143I GATC 1 cut(s) 298
BspACI CCGC 2 cut(s) 1058, 1097
BspANI GGCC 2 cut(s) 276, 968
BspHI TCATGA 2 cut(s) 961, 1319
BspLI GGNNCC 6 cut(s) 9, 28, 452, 640, 664, 982
BspMAI CTGCAG 1 cut(s) 1270
BsrDI GCAATG 3 cut(s) 97, 816, 847
BsrFI RCCGGY 1 cut(s) 977
BsrI ACTGG 4 cut(s) 248, 478, 833, 996
BssAI RCCGGY 1 cut(s) 977
BssECI CCNNGG 4 cut(s) 394, 443, 634, 1242
BssMI GATC 1 cut(s) 298
BssT1I CCWWGG 2 cut(s) 394, 443
Bst2UI CCWGG 1 cut(s) 1244
Bst4CI ACNGT 7 cut(s) 213, 550, 620, 726, 733, 787, 1289
Bst6I CTCTTC 1 cut(s) 642
BstDEI CTNAG 1 cut(s) 872
BstEII GGTNACC 1 cut(s) 124
BstF5I GGATG 1 cut(s) 537
BstH2I RGCGCY 1 cut(s) 1250
BstHHI GCGC 1 cut(s) 1249
BstKTI GATC 1 cut(s) 301
BstMAI GTCTC 1 cut(s) 522
BstMBI GATC 1 cut(s) 298
BstMWI GCNNNNNNNGC 5 cut(s) 413, 671, 680, 1253, 1262
BstNI CCWGG 1 cut(s) 1244
BstNSI RCATGY 2 cut(s) 207, 778
BstPI GGTNACC 1 cut(s) 124
BstSCI CCNGG 2 cut(s) 634, 1242
BstSFI CTRYAG 2 cut(s) 546, 1266
BstV1I GCAGC 4 cut(s) 491, 895, 1091, 1252
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 298
BstYI RGATCY 1 cut(s) 298
BsuI GTATCC 2 cut(s) 818, 943
BsuRI GGCC 2 cut(s) 276, 968
BtsCI GGATG 1 cut(s) 537
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 5 cut(s) 61, 241, 471, 783, 1294
CciI TCATGA 2 cut(s) 961, 1319
CfoI GCGC 1 cut(s) 1249
Cfr10I RCCGGY 1 cut(s) 977
Cfr13I GGNCC 3 cut(s) 451, 878, 967
Csp6I GTAC 2 cut(s) 145, 781
CspAI ACCGGT 1 cut(s) 977
CviAII CATG 9 cut(s) 99, 204, 304, 379, 424, 775, 962, 1093, 1320
CviQI GTAC 2 cut(s) 145, 781
DdeI CTNAG 1 cut(s) 872
DpnI GATC 1 cut(s) 300
DpnII GATC 1 cut(s) 298
EaeI YGGCCR 1 cut(s) 274
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
EciI GGCGGA 1 cut(s) 1112
Eco130I CCWWGG 2 cut(s) 394, 443
Eco47I GGWCC 2 cut(s) 451, 878
Eco57I CTGAAG 2 cut(s) 152, 765
Eco88I CYCGRG 1 cut(s) 31
Eco91I GGTNACC 1 cut(s) 124
EcoO109I RGGNCCY 1 cut(s) 967
EcoO65I GGTNACC 1 cut(s) 124
EcoRI GAATTC 1 cut(s) 428
EcoRII CCWGG 1 cut(s) 1242
EcoT14I CCWWGG 2 cut(s) 394, 443
ErhI CCWWGG 2 cut(s) 394, 443
FaeI CATG 9 cut(s) 102, 207, 307, 382, 427, 778, 965, 1096, 1323
FaqI GGGAC 1 cut(s) 617
FatI CATG 9 cut(s) 98, 203, 303, 378, 423, 774, 961, 1092, 1319
FauI CCCGC 1 cut(s) 1051
FauNDI CATATG 2 cut(s) 522, 835
Fnu4HI GCNGC 4 cut(s) 480, 909, 1080, 1266
FokI GGATG 1 cut(s) 524
Fsp4HI GCNGC 4 cut(s) 480, 909, 1080, 1266
FspBI CTAG 1 cut(s) 971
GlaI GCGC 1 cut(s) 1248
GluI GCNGC 4 cut(s) 480, 909, 1080, 1266
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 1250
HaeIII GGCC 2 cut(s) 276, 968
HapII CCGG 2 cut(s) 636, 978
HhaI GCGC 1 cut(s) 1249
Hin1II CATG 9 cut(s) 102, 207, 307, 382, 427, 778, 965, 1096, 1323
Hin6I GCGC 1 cut(s) 1247
HinP1I GCGC 1 cut(s) 1247
HincII GTYRAC 2 cut(s) 729, 1285
HindII GTYRAC 2 cut(s) 729, 1285
HinfI GANTC 9 cut(s) 95, 291, 439, 528, 958, 1032, 1044, 1173, 1316
HpaII CCGG 2 cut(s) 636, 978
HphI GGTGA 3 cut(s) 59, 136, 547
Hpy166II GTNNAC 3 cut(s) 729, 783, 1285
Hpy188I TCNGA 5 cut(s) 7, 290, 296, 662, 882
Hpy188III TCNNGA 4 cut(s) 705, 962, 1024, 1320
Hpy8I GTNNAC 3 cut(s) 729, 783, 1285
HpyAV CCTTC 3 cut(s) 493, 757, 1049
HpyCH4III ACNGT 7 cut(s) 213, 550, 620, 726, 733, 787, 1289
HpyCH4IV ACGT 1 cut(s) 341
HpyCH4V TGCA 5 cut(s) 674, 778, 821, 1268, 1313
HpyF10VI GCNNNNNNNGC 5 cut(s) 413, 671, 680, 1253, 1262
HpyF3I CTNAG 1 cut(s) 872
HpySE526I ACGT 1 cut(s) 341
Hsp92II CATG 9 cut(s) 102, 207, 307, 382, 427, 778, 965, 1096, 1323
HspAI GCGC 1 cut(s) 1247
Kzo9I GATC 1 cut(s) 298
LmnI GCTCC 3 cut(s) 476, 662, 1118
Lsp1109I GCAGC 4 cut(s) 491, 895, 1091, 1252
LweI GCATC 1 cut(s) 108
MaeI CTAG 1 cut(s) 971
MaeII ACGT 1 cut(s) 341
MaeIII GTNAC 1 cut(s) 124
MalI GATC 1 cut(s) 300
MboI GATC 1 cut(s) 298
MboII GAAGA 9 cut(s) 26, 158, 333, 359, 478, 659, 843, 1041, 1230
MfeI CAATTG 2 cut(s) 136, 684
MflI RGATCY 1 cut(s) 298
MlsI TGGCCA 1 cut(s) 276
MluCI AATT 5 cut(s) 136, 428, 675, 684, 1295
MluNI TGGCCA 1 cut(s) 276
MlyI GAGTC 1 cut(s) 1182
MmeI TCCRAC 1 cut(s) 167
Mox20I TGGCCA 1 cut(s) 276
MscI TGGCCA 1 cut(s) 276
MseI TTAA 4 cut(s) 594, 669, 735, 1005
Msp20I TGGCCA 1 cut(s) 276
MspI CCGG 2 cut(s) 636, 978
MspR9I CCNGG 2 cut(s) 636, 1244
MunI CAATTG 2 cut(s) 136, 684
Mva1269I GAATGC 1 cut(s) 497
MvaI CCWGG 1 cut(s) 1244
MwoI GCNNNNNNNGC 5 cut(s) 413, 671, 680, 1253, 1262
NciI CCSGG 1 cut(s) 636
NdeI CATATG 2 cut(s) 522, 835
NdeII GATC 1 cut(s) 298
NlaIII CATG 9 cut(s) 102, 207, 307, 382, 427, 778, 965, 1096, 1323
NlaIV GGNNCC 6 cut(s) 9, 28, 452, 640, 664, 982
NmuCI GTSAC 1 cut(s) 124
NspI RCATGY 2 cut(s) 207, 778
PagI TCATGA 2 cut(s) 961, 1319
PcsI WCGNNNNNNNCGW 1 cut(s) 501
PctI GAATGC 1 cut(s) 497
PfeI GAWTC 8 cut(s) 95, 291, 439, 528, 958, 1032, 1044, 1316
PflMI CCANNNNNTGG 1 cut(s) 358
PinAI ACCGGT 1 cut(s) 977
PkrI GCNGC 4 cut(s) 481, 910, 1081, 1267
PleI GAGTC 1 cut(s) 1181
PpsI GAGTC 1 cut(s) 1181
Psp6I CCWGG 1 cut(s) 1242
PspEI GGTNACC 1 cut(s) 124
PspGI CCWGG 1 cut(s) 1242
PspN4I GGNNCC 6 cut(s) 9, 28, 452, 640, 664, 982
PspPI GGNCC 3 cut(s) 451, 878, 967
PstI CTGCAG 1 cut(s) 1270
PsuI RGATCY 1 cut(s) 298
RsaI GTAC 2 cut(s) 146, 782
RsaNI GTAC 2 cut(s) 145, 781
SaqAI TTAA 4 cut(s) 594, 669, 735, 1005
SatI GCNGC 4 cut(s) 480, 909, 1080, 1266
Sau3AI GATC 1 cut(s) 298
Sau96I GGNCC 3 cut(s) 451, 878, 967
SchI GAGTC 1 cut(s) 1182
ScrFI CCNGG 2 cut(s) 636, 1244
SfaNI GCATC 1 cut(s) 108
SfcI CTRYAG 2 cut(s) 546, 1266
SinI GGWCC 2 cut(s) 451, 878
SmlI CTYRAG 2 cut(s) 410, 1022
SmoI CTYRAG 2 cut(s) 410, 1022
Sse9I AATT 5 cut(s) 136, 428, 675, 684, 1295
SsiI CCGC 2 cut(s) 1058, 1097
SspMI CTAG 1 cut(s) 971
StyD4I CCNGG 2 cut(s) 634, 1242
StyI CCWWGG 2 cut(s) 394, 443
TaaI ACNGT 7 cut(s) 213, 550, 620, 726, 733, 787, 1289
TaiI ACGT 1 cut(s) 344
TaqI TCGA 1 cut(s) 1042
TasI AATT 5 cut(s) 136, 428, 675, 684, 1295
TatI WGTACW 1 cut(s) 780
TfiI GAWTC 8 cut(s) 95, 291, 439, 528, 958, 1032, 1044, 1316
Tru1I TTAA 4 cut(s) 594, 669, 735, 1005
Tru9I TTAA 4 cut(s) 594, 669, 735, 1005
TscAI CASTG 5 cut(s) 61, 248, 478, 790, 1294
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 4 cut(s) 479, 908, 1079, 1265
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 9 cut(s) 87, 150, 376, 478, 843, 950, 1081, 1156, 1308
TspRI CASTG 5 cut(s) 61, 248, 478, 790, 1294
Van91I CCANNNNNTGG 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 451, 878
XapI RAATTY 1 cut(s) 428
XceI RCATGY 2 cut(s) 207, 778
XspI CTAG 1 cut(s) 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.