RLG00000004406

Proline transporter 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0019705
Physical Location & Seq
Reverse (-)
59099163 .. 59102134
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004406

Sequence Viewer

Length: 1314 bp
ATGGAGGAAACAGAGGGAACCCCAAGTAAGCACAAAGCTTTGAGCAACGAATATGCCCGCCAATCTGCTCATATCATAGGCAATGATTCATGGCAACAAGTTGGGTTGATGCTGACAACAAGCTTCAATTGTGGGTACATATTGAGCTTTTCAAATCTTCAATTGAAGCCTTTGGGTTGGGCATGGGGTATCACATGCCTGCTGATTTTTGGGCTCTATACTTTATATGCCAACTGGCTTATTGCTGCTTTTCACGTCATCAATGGCCAAAGGTTCATTAGATACAGAGATCTCATGGGCTATACATGTGGGAGAAAAATGTATTACATCACATTTTTCCTCCAATACCTGGTCATTCTTCTTACAAACACGGGTTTCATTCTCCTTGGGGGAAAAGCGCTCAAGGCGATAAATGCAGAGTTCAGCGACTCTCCCTGGAGGCTCCAATACTTTATCATTCTCACCGGAGTAGCCTTCTTCATTTTTGCATTTTGCGTTCCTACAATGTCTGATATGGGAATTTGGCTCATCCCCTCTACAATTGTCACCTTTGTTTATGTAATCATACTATTGGTGGTACTAATTGGCGATGGAAAAGCTAACACAAACCTTGATTATCATGTCAGCGGAAGCAAAGAAGACAGGGTGTTCAAAGCCCTTAATGCAATTTCAGCAATTGTTGTCTGCAGTAGTTCAGGCTTAGTACCAGAAATACAGTCAACTCTACGGAAGCCTGCAGTGGCAAATATGAGGAAGGCTCTGTACATGCAGTTTACTGTGGGGCTCCTCTTTTACTATGGAGTTACCATAGCAGGATATTGGGCGTTTGGCTCAAAAGTATCTGAATATCTCCCGAATCAGCTAAGTGGTCCTAGATGGATCAAGGTCTTCATAAACGCTGCCGTTTTTGTACAATCCATAGTCTCCCAACATACATTTGTTATACCAATTCATGAGGCCCTTGATACCAAGTTCCTCCACCTTGATAAGAGCATTAACTCGGCAGAAAATCTCAAGAGGAGATTTTGCATTCGGGCCCTCCTCTTCACCGGAAATACGCTTGTGACAGCAGCTATTCCTTTCATGGGGGACTTTGTAAACTTGTTTGGAGCATTAGCACTCATTCCTCTGACTCTCGTGTTCCCAAGCTTGATTTTCATTAATGTCAAGGGAAAGACAGCTAGACCACATCAGAACTTATGGCATTGGTTCAACATTATTACTTTTTCTCTCATTGCGTTGGCTGCCACAATTTCTGCAGTAAGATTGATAGTCCACAATGTTGAGAAATATAGTTTATTTTCAAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aa_trans

Protein Analysis

438

Amino Acids

49.07

Weight (kDa)

9.41

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aa_trans PF01490 28 - 421 2.5e-60 Transmembrane amino acid transporter protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g06641 FvH4_5g06641
rosa_chinensis RchiOBHm_Chr7g0192041
rosa_laevigata RLG00000004406
rosa_rugosa Rorug06G0512600
rosa_samantha Rh7AG123000
rosa_wichuraiana Rw7G010470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 349
AciI CCGC 2 cut(s) 58, 627
AclWI GGATC 1 cut(s) 887
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 519, 1306
AfaI GTAC 5 cut(s) 137, 579, 705, 764, 912
AfeI AGCGCT 1 cut(s) 399
AfiI CCNNNNNNNGG 2 cut(s) 349, 1085
AflIII ACRYGT 1 cut(s) 305
AgsI TTSAA 7 cut(s) 127, 153, 161, 166, 652, 1213, 1305
AjiI CACGTC 1 cut(s) 256
AjnI CCWGG 2 cut(s) 348, 434
AluBI AGCT 8 cut(s) 38, 123, 147, 599, 862, 1073, 1149, 1181
AluI AGCT 8 cut(s) 38, 123, 147, 599, 862, 1073, 1149, 1181
Alw26I GTCTC 1 cut(s) 928
AlwI GGATC 1 cut(s) 887
Aor51HI AGCGCT 1 cut(s) 399
AoxI GGCC 3 cut(s) 265, 957, 1035
ApaI GGGCCC 1 cut(s) 1039
ApeKI GCWGC 4 cut(s) 245, 899, 1070, 1244
ApoI RAATTY 2 cut(s) 519, 1306
AseI ATTAAT 1 cut(s) 1161
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 4 cut(s) 869, 958, 1035, 1036
AsuHPI GGTGA 3 cut(s) 454, 538, 1039
AvaII GGWCC 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 1039
BalI TGGCCA 1 cut(s) 267
BanII GRGCYC 3 cut(s) 216, 786, 1039
BarI GAAGNNNNNNTAC 2 cut(s) 746, 778
BauI CACGAG 1 cut(s) 1136
BbsI GAAGAC 2 cut(s) 645, 880
BbvI GCAGC 4 cut(s) 232, 886, 1082, 1231
BccI CCATC 2 cut(s) 584, 870
BceAI ACGGC 1 cut(s) 887
BciT130I CCWGG 2 cut(s) 350, 436
BcoDI GTCTC 1 cut(s) 928
BfaI CTAG 2 cut(s) 873, 1182
BfmI CTRYAG 3 cut(s) 685, 735, 1257
BfoI RGCGCY 1 cut(s) 401
BglII AGATCT 1 cut(s) 289
BisI GCNGC 4 cut(s) 246, 900, 1071, 1245
BlsI GCNGC 4 cut(s) 247, 901, 1072, 1246
Bme1390I CCNGG 2 cut(s) 350, 436
Bme18I GGWCC 1 cut(s) 869
BmgBI CACGTC 1 cut(s) 256
BmgT120I GGNCC 4 cut(s) 869, 958, 1035, 1036
BmiI GGNNCC 4 cut(s) 19, 443, 785, 1037
BmrFI CCNGG 2 cut(s) 350, 436
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 645, 880
BplI GAGNNNNNCTC 2 cut(s) 742, 774
BpmI CTGGAG 1 cut(s) 457
BpuEI CTTGAG 2 cut(s) 386, 998
BsaJI CCNNGG 2 cut(s) 385, 434
BsaWI WCCGGW 2 cut(s) 464, 1049
Bsc4I CCNNNNNNNGG 2 cut(s) 349, 1085
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 2 cut(s) 88, 1233
BseBI CCWGG 2 cut(s) 350, 436
BseDI CCNNGG 2 cut(s) 385, 434
BseGI GGATG 1 cut(s) 528
BseLI CCNNNNNNNGG 2 cut(s) 349, 1085
BseMI GCAATG 2 cut(s) 88, 1233
BseNI ACTGG 1 cut(s) 239
BseRI GAGGAG 3 cut(s) 776, 1031, 1033
BseSI GKGCMC 1 cut(s) 1039
BseXI GCAGC 4 cut(s) 232, 886, 1082, 1231
BshFI GGCC 3 cut(s) 267, 959, 1037
BsiSI CCGG 2 cut(s) 465, 1050
BslFI GGGAC 1 cut(s) 1103
BslI CCNNNNNNNGG 2 cut(s) 349, 1085
BsmAI GTCTC 1 cut(s) 928
BsmFI GGGAC 1 cut(s) 1103
BsmI GAATGC 1 cut(s) 1029
BsnI GGCC 3 cut(s) 267, 959, 1037
Bsp120I GGGCCC 1 cut(s) 1035
Bsp1286I GDGCHC 3 cut(s) 216, 786, 1039
Bsp1407I TGTACA 2 cut(s) 762, 910
Bsp143I GATC 2 cut(s) 289, 879
BspACI CCGC 2 cut(s) 58, 627
BspANI GGCC 3 cut(s) 267, 959, 1037
BspHI TCATGA 2 cut(s) 952, 1310
BspLI GGNNCC 4 cut(s) 19, 443, 785, 1037
BspMAI CTGCAG 3 cut(s) 689, 739, 1261
BspPI GGATC 1 cut(s) 887
BsrDI GCAATG 2 cut(s) 88, 1233
BsrGI TGTACA 2 cut(s) 762, 910
BsrI ACTGG 1 cut(s) 239
BssECI CCNNGG 2 cut(s) 385, 434
BssMI GATC 2 cut(s) 289, 879
BssSI CACGAG 1 cut(s) 1136
BssT1I CCWWGG 1 cut(s) 385
Bst2BI CACGAG 1 cut(s) 1136
Bst2UI CCWGG 2 cut(s) 350, 436
Bst4CI ACNGT 2 cut(s) 717, 778
Bst6I CTCTTC 1 cut(s) 1049
BstAUI TGTACA 2 cut(s) 762, 910
BstC8I GCNNGC 3 cut(s) 58, 200, 735
BstDEI CTNAG 2 cut(s) 700, 863
BstF5I GGATG 1 cut(s) 528
BstH2I RGCGCY 1 cut(s) 401
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 2 cut(s) 292, 882
BstMAI GTCTC 1 cut(s) 928
BstMBI GATC 2 cut(s) 289, 879
BstMWI GCNNNNNNNGC 5 cut(s) 404, 413, 662, 671, 1244
BstNI CCWGG 2 cut(s) 350, 436
BstNSI RCATGY 3 cut(s) 198, 309, 769
BstSCI CCNGG 2 cut(s) 348, 434
BstSFI CTRYAG 3 cut(s) 685, 735, 1257
BstSLI GKGCMC 1 cut(s) 1039
BstV1I GCAGC 4 cut(s) 232, 886, 1082, 1231
BstV2I GAAGAC 2 cut(s) 645, 880
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 3 cut(s) 267, 959, 1037
BtgZI GCGATG 1 cut(s) 603
BtrI CACGTC 1 cut(s) 256
BtsCI GGATG 1 cut(s) 528
BtsI GCAGTG 1 cut(s) 744
BtsIMutI CAGTG 1 cut(s) 744
Cac8I GCNNGC 3 cut(s) 58, 200, 735
CciI TCATGA 2 cut(s) 952, 1310
CfoI GCGC 1 cut(s) 400
Cfr13I GGNCC 4 cut(s) 869, 958, 1035, 1036
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 5 cut(s) 136, 578, 704, 763, 911
CviQI GTAC 5 cut(s) 136, 578, 704, 763, 911
DdeI CTNAG 2 cut(s) 700, 863
DpnI GATC 2 cut(s) 291, 881
DpnII GATC 2 cut(s) 289, 879
EaeI YGGCCR 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 1049
EarI CTCTTC 1 cut(s) 1049
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 3 cut(s) 216, 786, 1039
Eco47I GGWCC 1 cut(s) 869
Eco47III AGCGCT 1 cut(s) 399
EcoO109I RGGNCCY 2 cut(s) 958, 1036
EcoRII CCWGG 2 cut(s) 348, 434
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 3 cut(s) 216, 786, 1039
ErhI CCWWGG 1 cut(s) 385
FaqI GGGAC 1 cut(s) 1103
FauI CCCGC 1 cut(s) 65
Fnu4HI GCNGC 4 cut(s) 246, 900, 1071, 1245
FokI GGATG 1 cut(s) 515
FriOI GRGCYC 3 cut(s) 216, 786, 1039
Fsp4HI GCNGC 4 cut(s) 246, 900, 1071, 1245
FspBI CTAG 2 cut(s) 873, 1182
GlaI GCGC 1 cut(s) 399
GluI GCNGC 4 cut(s) 246, 900, 1071, 1245
GsuI CTGGAG 1 cut(s) 457
HaeII RGCGCY 1 cut(s) 401
HaeIII GGCC 3 cut(s) 267, 959, 1037
HapII CCGG 2 cut(s) 465, 1050
HhaI GCGC 1 cut(s) 400
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HindIII AAGCTT 3 cut(s) 36, 121, 1147
HinfI GANTC 4 cut(s) 86, 428, 856, 1132
HpaII CCGG 2 cut(s) 465, 1050
HphI GGTGA 3 cut(s) 454, 538, 1039
Hpy166II GTNNAC 4 cut(s) 720, 774, 1099, 1276
Hpy188I TCNGA 4 cut(s) 511, 844, 1131, 1194
Hpy188III TCNNGA 4 cut(s) 853, 953, 1015, 1311
Hpy8I GTNNAC 4 cut(s) 720, 774, 1099, 1276
HpyAV CCTTC 2 cut(s) 484, 748
HpyCH4III ACNGT 2 cut(s) 717, 778
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 8 cut(s) 416, 488, 665, 687, 737, 769, 1029, 1259
HpyF10VI GCNNNNNNNGC 5 cut(s) 404, 413, 662, 671, 1244
HpyF3I CTNAG 2 cut(s) 700, 863
HpySE526I ACGT 1 cut(s) 255
HspAI GCGC 1 cut(s) 398
Kzo9I GATC 2 cut(s) 289, 879
LmnI GCTCC 3 cut(s) 447, 789, 1109
Lsp1109I GCAGC 4 cut(s) 232, 886, 1082, 1231
LweI GCATC 1 cut(s) 99
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 2 cut(s) 873, 1182
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 544, 802, 1063
MalI GATC 2 cut(s) 291, 881
MboI GATC 2 cut(s) 289, 879
MboII GAAGA 6 cut(s) 149, 350, 469, 650, 880, 1036
MfeI CAATTG 4 cut(s) 127, 161, 540, 675
MflI RGATCY 1 cut(s) 289
MhlI GDGCHC 3 cut(s) 216, 786, 1039
MlsI TGGCCA 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 267
MlyI GAGTC 2 cut(s) 422, 1126
Mox20I TGGCCA 1 cut(s) 267
MscI TGGCCA 1 cut(s) 267
MseI TTAA 3 cut(s) 660, 996, 1161
Msp20I TGGCCA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 627
MspI CCGG 2 cut(s) 465, 1050
MspR9I CCNGG 2 cut(s) 350, 436
MunI CAATTG 4 cut(s) 127, 161, 540, 675
Mva1269I GAATGC 1 cut(s) 1029
MvaI CCWGG 2 cut(s) 350, 436
MwoI GCNNNNNNNGC 5 cut(s) 404, 413, 662, 671, 1244
NdeII GATC 2 cut(s) 289, 879
NlaIV GGNNCC 4 cut(s) 19, 443, 785, 1037
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 2 cut(s) 544, 1063
NspI RCATGY 3 cut(s) 198, 309, 769
PagI TCATGA 2 cut(s) 952, 1310
PciI ACATGT 1 cut(s) 305
PctI GAATGC 1 cut(s) 1029
PfeI GAWTC 2 cut(s) 86, 856
PflMI CCANNNNNTGG 1 cut(s) 349
PkrI GCNGC 4 cut(s) 247, 901, 1072, 1246
PleI GAGTC 2 cut(s) 422, 1126
PpsI GAGTC 2 cut(s) 422, 1126
PscI ACATGT 1 cut(s) 305
PshBI ATTAAT 1 cut(s) 1161
Psp6I CCWGG 2 cut(s) 348, 434
PspGI CCWGG 2 cut(s) 348, 434
PspN4I GGNNCC 4 cut(s) 19, 443, 785, 1037
PspOMI GGGCCC 1 cut(s) 1035
PspPI GGNCC 4 cut(s) 869, 958, 1035, 1036
PstI CTGCAG 3 cut(s) 689, 739, 1261
PsuI RGATCY 1 cut(s) 289
RsaI GTAC 5 cut(s) 137, 579, 705, 764, 912
RsaNI GTAC 5 cut(s) 136, 578, 704, 763, 911
SaqAI TTAA 3 cut(s) 660, 996, 1161
SatI GCNGC 4 cut(s) 246, 900, 1071, 1245
Sau3AI GATC 2 cut(s) 289, 879
Sau96I GGNCC 4 cut(s) 869, 958, 1035, 1036
SchI GAGTC 2 cut(s) 422, 1126
ScrFI CCNGG 2 cut(s) 350, 436
SduI GDGCHC 3 cut(s) 216, 786, 1039
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 1 cut(s) 99
SfcI CTRYAG 3 cut(s) 685, 735, 1257
SinI GGWCC 1 cut(s) 869
SmlI CTYRAG 2 cut(s) 401, 1013
SmoI CTYRAG 2 cut(s) 401, 1013
SsiI CCGC 2 cut(s) 58, 627
SspMI CTAG 2 cut(s) 873, 1182
StyD4I CCNGG 2 cut(s) 348, 434
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 2 cut(s) 717, 778
TaiI ACGT 1 cut(s) 258
TatI WGTACW 2 cut(s) 762, 910
TfiI GAWTC 2 cut(s) 86, 856
Tru1I TTAA 3 cut(s) 660, 996, 1161
Tru9I TTAA 3 cut(s) 660, 996, 1161
TscAI CASTG 1 cut(s) 744
TseFI GTSAC 2 cut(s) 544, 1063
TseI GCWGC 4 cut(s) 245, 899, 1070, 1244
Tsp45I GTSAC 2 cut(s) 544, 1063
TspDTI ATGAA 9 cut(s) 78, 265, 367, 469, 880, 941, 1072, 1147, 1299
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 1 cut(s) 744
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 869
VspI ATTAAT 1 cut(s) 1161
XapI RAATTY 2 cut(s) 519, 1306
XceI RCATGY 3 cut(s) 198, 309, 769
XspI CTAG 2 cut(s) 873, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.