FvH4_5g13532

COBRA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0021075
Physical Location & Seq
Forward (+)
7658719 .. 7660563
1845 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g13532.t1

Sequence Viewer

Length: 909 bp
ATGGGGATTACCAAGTCTAAAAGAAGCACATTATTTGCATCTATCATGATTTTGGCAATATTTTCTTCTGCAGTGGCTTATGATCCCTTTGATCCCAATGGAAGCATCAATATCAAGTGGGATGTTTTGTCTTGGACCCCTGATGGCTATGTGGCAGCAGTGTCAATAAGCAACAACCAAATGTACCGGCACATCACAAGCCCCGGATGGAGCTTAGGATGGACATGGGCAAAGAAGGAAGTGATCTGGTCCATGGTGGGAGCTAAAGTCGCCGATCAAGGGGACTGTTCGAAATTCAAAGGGAACACCCCACTTTGTTGTGAGAAAACTCCAACAGTTATTGACTTACTTCCCGGAGTTCCTTACAACCAACAGTTCACCAATTGCTGCAAGGGTGGTGTTTTGACATCATTGGGACAAGACCCTTCTAGTGCAGTCTCAGCATTTCAGATGAGTGTTGGCCTTGCAGGCACTTCAAATAAAACCGTAAGGCCTCCGAAGAAATTCTTTTTGCTTGGTCCTGGATCAGGCTATACTTGTAGTGCCGCCAACATTGTGCCATCCTCGGTTACCTTGTCATCTGACCACCGGAGTAAAACTAGAGCAATGAATGACACTGGTATGTTTTATGGCATAAAAACTGTGAATGACCTAATAAGGGAAGCTGGGCCTAAAGGATATATCTATACAGAGTTGATTCTTAAGAAGGACAAGAACACATTCACATTGGATCAAGGCTGGGCATTTCCTCTCAGGGTTTACTTCAATGGTGATGAGTGCTTGATGCCCCTTCCTGATCTATACCCATATCTACCCAACTCTGCTTTTCATAATTCATATCCAATTTTTACATTATCTTCTACAATATTGGCTACCCTTTTGGTTTTCGTATTGCTAGTCTTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
COBRA

Protein Analysis

303

Amino Acids

33.02

Weight (kDa)

8.86

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COBRA PF04833 50 - 204 2.4e-56 COBRA-like protein
COB_C PF25079 200 - 269 1.2e-11 COBRA family C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g13532
rosa_multiflora Rmu_co8312737.1_g000001 Rmu_sc0000951.1_g000004 Rmu_sc0000951.1_g000007 Rmu_sc0016134.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 546
AclWI GGATC 4 cut(s) 77, 86, 532, 738
AcsI RAATTY 2 cut(s) 293, 503
AfaI GTAC 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 279, 527, 658
AflII CTTAAG 1 cut(s) 701
AgsI TTSAA 3 cut(s) 298, 477, 766
AjnI CCWGG 1 cut(s) 520
AluBI AGCT 3 cut(s) 213, 263, 665
AluI AGCT 3 cut(s) 213, 263, 665
Alw26I GTCTC 1 cut(s) 442
AlwI GGATC 4 cut(s) 77, 86, 532, 738
AoxI GGCC 3 cut(s) 460, 491, 668
ApeKI GCWGC 2 cut(s) 155, 387
ApoI RAATTY 2 cut(s) 293, 503
Asp700I GAANNNNTTC 2 cut(s) 503, 719
AspS9I GGNCC 4 cut(s) 135, 249, 518, 668
AsuC2I CCSGG 2 cut(s) 204, 354
AsuHPI GGTGA 2 cut(s) 370, 782
AsuII TTCGAA 1 cut(s) 290
AvaII GGWCC 3 cut(s) 135, 249, 518
BbvI GCAGC 2 cut(s) 167, 374
BccI CCATC 4 cut(s) 137, 201, 213, 568
BciT130I CCWGG 1 cut(s) 522
BcnI CCSGG 2 cut(s) 204, 354
BcoDI GTCTC 1 cut(s) 442
BfaI CTAG 3 cut(s) 429, 600, 896
BfmI CTRYAG 1 cut(s) 69
BfrI CTTAAG 1 cut(s) 701
BglI GCCNNNNNGGC 1 cut(s) 468
BisI GCNGC 3 cut(s) 156, 388, 546
BlsI GCNGC 3 cut(s) 157, 389, 547
Bme1390I CCNGG 3 cut(s) 204, 354, 522
Bme18I GGWCC 3 cut(s) 135, 249, 518
BmgT120I GGNCC 4 cut(s) 135, 249, 518, 668
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 3 cut(s) 204, 354, 522
BmsI GCATC 3 cut(s) 47, 114, 774
Bpu10I CCTNAGC 1 cut(s) 214
Bpu14I TTCGAA 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 204, 354
BsaJI CCNNGG 3 cut(s) 202, 252, 564
BsaWI WCCGGW 1 cut(s) 588
BsaXI ACNNNNNCTCC 2 cut(s) 252, 282
Bsc4I CCNNNNNNNGG 3 cut(s) 279, 527, 658
Bse118I RCCGGY 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 622
Bse3DI GCAATG 1 cut(s) 612
BseBI CCWGG 1 cut(s) 522
BseDI CCNNGG 3 cut(s) 202, 252, 564
BseGI GGATG 4 cut(s) 127, 212, 224, 560
BseLI CCNNNNNNNGG 3 cut(s) 279, 527, 658
BseMI GCAATG 1 cut(s) 612
BseMII CTCAG 2 cut(s) 453, 766
BseNI ACTGG 1 cut(s) 622
BseXI GCAGC 2 cut(s) 167, 374
BseYI CCCAGC 2 cut(s) 665, 738
BsgI GTGCAG 1 cut(s) 453
BshFI GGCC 3 cut(s) 462, 493, 670
BsiSI CCGG 4 cut(s) 187, 204, 354, 589
BslFI GGGAC 2 cut(s) 296, 429
BslI CCNNNNNNNGG 3 cut(s) 279, 527, 658
BsmAI GTCTC 1 cut(s) 442
BsmFI GGGAC 2 cut(s) 296, 429
BsnI GGCC 3 cut(s) 462, 493, 670
Bsp119I TTCGAA 1 cut(s) 290
Bsp143I GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 1 cut(s) 546
BspANI GGCC 3 cut(s) 462, 493, 670
BspCNI CTCAG 2 cut(s) 452, 765
BspHI TCATGA 1 cut(s) 45
BspLI GGNNCC 1 cut(s) 137
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 4 cut(s) 77, 86, 532, 738
BspT104I TTCGAA 1 cut(s) 290
BspTI CTTAAG 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 612
BsrFI RCCGGY 1 cut(s) 186
BsrI ACTGG 1 cut(s) 622
BssAI RCCGGY 1 cut(s) 186
BssECI CCNNGG 3 cut(s) 202, 252, 564
BssMI GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 522
Bst4CI ACNGT 6 cut(s) 287, 337, 375, 487, 643, 906
BstAFI CTTAAG 1 cut(s) 701
BstBI TTCGAA 1 cut(s) 290
BstC8I GCNNGC 1 cut(s) 469
BstDEI CTNAG 3 cut(s) 214, 439, 752
BstDSI CCRYGG 1 cut(s) 252
BstEII GGTNACC 1 cut(s) 568
BstENI CCTNNNNNAGG 1 cut(s) 525
BstF5I GGATG 4 cut(s) 127, 212, 224, 560
BstKTI GATC 7 cut(s) 85, 94, 246, 277, 527, 733, 799
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
BstMWI GCNNNNNNNGC 3 cut(s) 269, 440, 468
BstNI CCWGG 1 cut(s) 522
BstPI GGTNACC 1 cut(s) 568
BstSCI CCNGG 3 cut(s) 202, 352, 520
BstSFI CTRYAG 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 167, 374
BsuRI GGCC 3 cut(s) 462, 493, 670
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 4 cut(s) 127, 212, 224, 560
BtsI GCAGTG 2 cut(s) 78, 165
BtsIMutI CAGTG 3 cut(s) 78, 165, 615
Cac8I GCNNGC 1 cut(s) 469
CciI TCATGA 1 cut(s) 45
Cfr10I RCCGGY 1 cut(s) 186
Cfr13I GGNCC 4 cut(s) 135, 249, 518, 668
Csp6I GTAC 1 cut(s) 184
CviAII CATG 3 cut(s) 46, 225, 253
CviQI GTAC 1 cut(s) 184
DdeI CTNAG 3 cut(s) 214, 439, 752
DpnI GATC 7 cut(s) 84, 93, 245, 276, 526, 732, 798
DpnII GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
Eco130I CCWWGG 1 cut(s) 252
Eco147I AGGCCT 1 cut(s) 493
Eco47I GGWCC 3 cut(s) 135, 249, 518
Eco91I GGTNACC 1 cut(s) 568
EcoNI CCTNNNNNAGG 1 cut(s) 525
EcoO65I GGTNACC 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 520
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 3 cut(s) 49, 228, 256
FalI AAGNNNNNCTT 2 cut(s) 491, 523
FaqI GGGAC 2 cut(s) 296, 429
FatI CATG 3 cut(s) 45, 224, 252
Fnu4HI GCNGC 3 cut(s) 156, 388, 546
FokI GGATG 4 cut(s) 134, 219, 231, 547
Fsp4HI GCNGC 3 cut(s) 156, 388, 546
FspBI CTAG 3 cut(s) 429, 600, 896
GluI GCNGC 3 cut(s) 156, 388, 546
GsaI CCCAGC 2 cut(s) 669, 742
HaeIII GGCC 3 cut(s) 462, 493, 670
HapII CCGG 4 cut(s) 187, 204, 354, 589
Hin1II CATG 3 cut(s) 49, 228, 256
HinfI GANTC 1 cut(s) 697
HpaII CCGG 4 cut(s) 187, 204, 354, 589
HphI GGTGA 2 cut(s) 370, 782
Hpy166II GTNNAC 2 cut(s) 378, 760
Hpy188I TCNGA 3 cut(s) 450, 498, 583
Hpy188III TCNNGA 2 cut(s) 46, 794
Hpy8I GTNNAC 2 cut(s) 378, 760
HpyAV CCTTC 4 cut(s) 229, 435, 700, 800
HpyCH4III ACNGT 6 cut(s) 287, 337, 375, 487, 643, 906
HpyCH4V TGCA 5 cut(s) 38, 71, 390, 434, 467
HpyF10VI GCNNNNNNNGC 3 cut(s) 269, 440, 468
HpyF3I CTNAG 3 cut(s) 214, 439, 752
Hsp92II CATG 3 cut(s) 49, 228, 256
Kzo9I GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
LmnI GCTCC 2 cut(s) 210, 260
Lsp1109I GCAGC 2 cut(s) 167, 374
LweI GCATC 3 cut(s) 47, 114, 774
MaeI CTAG 3 cut(s) 429, 600, 896
MaeIII GTNAC 1 cut(s) 568
MalI GATC 7 cut(s) 84, 93, 245, 276, 526, 732, 798
MboI GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
MboII GAAGA 3 cut(s) 57, 511, 849
MfeI CAATTG 1 cut(s) 382
MluCI AATT 5 cut(s) 293, 382, 503, 832, 843
MmeI TCCRAC 1 cut(s) 356
MnlI CCTC 3 cut(s) 504, 574, 759
MroXI GAANNNNTTC 2 cut(s) 503, 719
MseI TTAA 1 cut(s) 702
MslI CAYNNNNRTG 1 cut(s) 620
MspCI CTTAAG 1 cut(s) 701
MspI CCGG 4 cut(s) 187, 204, 354, 589
MspR9I CCNGG 3 cut(s) 204, 354, 522
MunI CAATTG 1 cut(s) 382
MvaI CCWGG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 269, 440, 468
NciI CCSGG 2 cut(s) 204, 354
NcoI CCATGG 1 cut(s) 252
NdeII GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
NlaIII CATG 3 cut(s) 49, 228, 256
NlaIV GGNNCC 1 cut(s) 137
NspV TTCGAA 1 cut(s) 290
PagI TCATGA 1 cut(s) 45
PceI AGGCCT 1 cut(s) 493
PdmI GAANNNNTTC 2 cut(s) 503, 719
PfeI GAWTC 1 cut(s) 697
PfoI TCCNGGA 2 cut(s) 352, 520
PkrI GCNGC 3 cut(s) 157, 389, 547
Psp6I CCWGG 1 cut(s) 520
PspEI GGTNACC 1 cut(s) 568
PspFI CCCAGC 2 cut(s) 665, 738
PspGI CCWGG 1 cut(s) 520
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 4 cut(s) 135, 249, 518, 668
PstI CTGCAG 1 cut(s) 73
RsaI GTAC 1 cut(s) 185
RsaNI GTAC 1 cut(s) 184
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 1 cut(s) 702
SatI GCNGC 3 cut(s) 156, 388, 546
Sau3AI GATC 7 cut(s) 82, 91, 243, 274, 524, 730, 796
Sau96I GGNCC 4 cut(s) 135, 249, 518, 668
ScrFI CCNGG 3 cut(s) 204, 354, 522
SetI ASST 5 cut(s) 215, 265, 575, 654, 667
SfaNI GCATC 3 cut(s) 47, 114, 774
SfcI CTRYAG 1 cut(s) 69
SfuI TTCGAA 1 cut(s) 290
SinI GGWCC 3 cut(s) 135, 249, 518
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 701
SmoI CTYRAG 1 cut(s) 701
Sse9I AATT 5 cut(s) 293, 382, 503, 832, 843
SseBI AGGCCT 1 cut(s) 493
SsiI CCGC 1 cut(s) 546
SspI AATATT 2 cut(s) 60, 867
SspMI CTAG 3 cut(s) 429, 600, 896
StuI AGGCCT 1 cut(s) 493
StyD4I CCNGG 3 cut(s) 202, 352, 520
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 6 cut(s) 287, 337, 375, 487, 643, 906
TaqI TCGA 1 cut(s) 290
TasI AATT 5 cut(s) 293, 382, 503, 832, 843
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 697
Tru1I TTAA 1 cut(s) 702
Tru9I TTAA 1 cut(s) 702
TscAI CASTG 3 cut(s) 78, 165, 622
TseI GCWGC 2 cut(s) 155, 387
TspDTI ATGAA 3 cut(s) 623, 818, 825
TspRI CASTG 3 cut(s) 78, 165, 622
Vha464I CTTAAG 1 cut(s) 701
VpaK11BI GGWCC 3 cut(s) 135, 249, 518
XagI CCTNNNNNAGG 1 cut(s) 525
XapI RAATTY 2 cut(s) 293, 503
XmnI GAANNNNTTC 2 cut(s) 503, 719
XspI CTAG 3 cut(s) 429, 600, 896
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.