FvH4_5g31252

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0018448
Physical Location & Seq
Reverse (-)
22143383 .. 22147340
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g31252.t1

Sequence Viewer

Length: 504 bp
ATGTCCTTAACGGGATGGGTTGGACCTAACCCATTCAAAGCTGGTAGCACGGAAGCGTCACCATACTCACGTTTCCCGCTTACAAAGCGGAAGCGCCTCGGAATCGCTCGGAAGCGGCCGGAAGCACGCTACTGGAAAAAGACGATCTGGGAGTGCTATGCAAAAGCATGGGCTTCCAAATCGGAATCGCGACGTAGGAAGCACAAGGTGGAGCTCTCGGCAGTCGTGGCAGAGGTGGAGGCTCGGCTTGCTTCTACTCTTCAAACGTATCGGACTGCAAACGCCGATCTTTCTCTAGCTGAAATAGAGTCGTCCGAGGCCACTAAACCTCCATCATCATTTCTGATGCGATCATCCGAGAGGAAGAACCTTGCAGACAAGGAGGCAGAAGCTCTCGAGAAGAAGATACAAGACCTCAAAACATGGAACTTGTTAGTGCATTTTGGAGAAGCACCTCTTCCAGCATTCCTAGTTACTGTTGCAGAAGTCATAACGAGAACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

168

Amino Acids

18.87

Weight (kDa)

9.91

Isoelectric Point (pI)

54.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018448)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g31252
prunus_persica Prupe.1G031600_v2.0.a1
rosa_laevigata RLG00000001725
rosa_roxburghii Rroxscaffold_3G00232780
rosa_rugosa Rorug07G0237500
rosa_samantha Rh7AG384800 Rh7BG371600 Rh7CG404100 Rh7DG383800
rosa_wichuraiana Rw7G032330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 190
AciI CCGC 3 cut(s) 77, 88, 115
AcoI YGGCCR 1 cut(s) 116
AdeI CACNNNGTG 1 cut(s) 208
AgsI TTSAA 2 cut(s) 37, 263
AluBI AGCT 4 cut(s) 41, 214, 299, 392
AluI AGCT 4 cut(s) 41, 214, 299, 392
Alw21I GWGCWC 1 cut(s) 216
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 2 cut(s) 116, 318
AspLEI GCGC 1 cut(s) 96
AspS9I GGNCC 1 cut(s) 23
AsuHPI GGTGA 1 cut(s) 51
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 216
BccI CCATC 2 cut(s) 9, 340
BfaI CTAG 2 cut(s) 296, 470
BfoI RGCGCY 1 cut(s) 97
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 23
BmsI GCATC 1 cut(s) 336
BsaJI CCNNGG 2 cut(s) 97, 315
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bse1I ACTGG 1 cut(s) 137
BseDI CCNNGG 2 cut(s) 97, 315
BseGI GGATG 2 cut(s) 20, 353
BseNI ACTGG 1 cut(s) 137
BseX3I CGGCCG 1 cut(s) 116
Bsh1236I CGCG 1 cut(s) 190
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 2 cut(s) 118, 320
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 119
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 2 cut(s) 118, 320
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 3 cut(s) 144, 286, 350
Bsp68I TCGCGA 1 cut(s) 190
BspACI CCGC 3 cut(s) 77, 88, 115
BspANI GGCC 2 cut(s) 118, 320
BspFNI CGCG 1 cut(s) 190
BsrI ACTGG 1 cut(s) 137
BssECI CCNNGG 2 cut(s) 97, 315
BssMI GATC 3 cut(s) 144, 286, 350
Bst4CI ACNGT 1 cut(s) 478
Bst6I CTCTTC 2 cut(s) 264, 462
BstC8I GCNNGC 2 cut(s) 127, 249
BstF5I GGATG 2 cut(s) 20, 353
BstFNI CGCG 1 cut(s) 190
BstH2I RGCGCY 1 cut(s) 97
BstHHI GCGC 1 cut(s) 96
BstKTI GATC 3 cut(s) 147, 289, 353
BstMBI GATC 3 cut(s) 144, 286, 350
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 3 cut(s) 85, 227, 248
BstUI CGCG 1 cut(s) 190
BstZI CGGCCG 1 cut(s) 116
BsuRI GGCC 2 cut(s) 118, 320
BtsCI GGATG 2 cut(s) 20, 353
BtuMI TCGCGA 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 127, 249
CfoI GCGC 1 cut(s) 96
Cfr13I GGNCC 1 cut(s) 23
CseI GACGC 1 cut(s) 45
CviAII CATG 2 cut(s) 168, 423
CviJI RGCY 9 cut(s) 41, 118, 173, 214, 242, 247, 299, 320, 392
CviKI_1 RGCY 9 cut(s) 41, 118, 173, 214, 242, 247, 299, 320, 392
DpnI GATC 3 cut(s) 146, 288, 352
DpnII GATC 3 cut(s) 144, 286, 350
DraIII CACNNNGTG 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 116
EagI CGGCCG 1 cut(s) 116
Eam1104I CTCTTC 2 cut(s) 264, 462
EarI CTCTTC 2 cut(s) 264, 462
Ecl136II GAGCTC 1 cut(s) 214
EclXI CGGCCG 1 cut(s) 116
Eco24I GRGCYC 1 cut(s) 216
Eco47I GGWCC 1 cut(s) 23
Eco52I CGGCCG 1 cut(s) 116
Eco53kI GAGCTC 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 216
FaeI CATG 2 cut(s) 171, 426
FaiI YATR 5 cut(s) 64, 159, 169, 424, 491
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FatI CATG 2 cut(s) 167, 422
FauI CCCGC 1 cut(s) 84
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 2 cut(s) 27, 340
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 2 cut(s) 296, 470
GlaI GCGC 1 cut(s) 95
GluI GCNGC 1 cut(s) 116
HaeII RGCGCY 1 cut(s) 97
HaeIII GGCC 2 cut(s) 118, 320
HapII CCGG 1 cut(s) 119
HgaI GACGC 1 cut(s) 45
HhaI GCGC 1 cut(s) 96
Hin1II CATG 2 cut(s) 171, 426
Hin6I GCGC 1 cut(s) 94
HinP1I GCGC 1 cut(s) 94
HinfI GANTC 3 cut(s) 102, 185, 308
HpaII CCGG 1 cut(s) 119
HphI GGTGA 1 cut(s) 51
Hpy188I TCNGA 7 cut(s) 101, 111, 184, 273, 316, 345, 358
Hpy188III TCNNGA 3 cut(s) 189, 395, 397
Hpy99I CGWCG 1 cut(s) 195
HpyCH4III ACNGT 1 cut(s) 478
HpyCH4IV ACGT 4 cut(s) 70, 193, 266, 500
HpyCH4V TGCA 5 cut(s) 161, 278, 374, 439, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 227, 248
HpySE526I ACGT 4 cut(s) 70, 193, 266, 500
Hsp92II CATG 2 cut(s) 171, 426
HspAI GCGC 1 cut(s) 94
Kzo9I GATC 3 cut(s) 144, 286, 350
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 5 cut(s) 27, 118, 132, 133, 474
LweI GCATC 1 cut(s) 336
MaeI CTAG 2 cut(s) 296, 470
MaeII ACGT 4 cut(s) 70, 193, 266, 500
MaeIII GTNAC 2 cut(s) 57, 472
MalI GATC 3 cut(s) 146, 288, 352
MboI GATC 3 cut(s) 144, 286, 350
MboII GAAGA 5 cut(s) 251, 376, 412, 415, 449
MhlI GDGCHC 1 cut(s) 216
MlyI GAGTC 1 cut(s) 317
MnlI CCTC 9 cut(s) 107, 226, 232, 310, 339, 354, 376, 425, 465
MseI TTAA 1 cut(s) 8
MspI CCGG 1 cut(s) 119
Mva1269I GAATGC 1 cut(s) 464
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 3 cut(s) 85, 227, 248
NdeII GATC 3 cut(s) 144, 286, 350
NlaIII CATG 2 cut(s) 171, 426
NmeAIII GCCGAG 2 cut(s) 197, 223
NmuCI GTSAC 1 cut(s) 57
NruI TCGCGA 1 cut(s) 190
PaeR7I CTCGAG 1 cut(s) 395
PctI GAATGC 1 cut(s) 464
PfeI GAWTC 2 cut(s) 102, 185
PkrI GCNGC 1 cut(s) 117
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 216
PspPI GGNCC 1 cut(s) 23
RruI TCGCGA 1 cut(s) 190
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 8
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 3 cut(s) 144, 286, 350
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 1 cut(s) 317
SduI GDGCHC 1 cut(s) 216
SfaNI GCATC 1 cut(s) 336
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 23
SlaI CTCGAG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
SsiI CCGC 3 cut(s) 77, 88, 115
SspMI CTAG 2 cut(s) 296, 470
SstI GAGCTC 1 cut(s) 216
TaaI ACNGT 1 cut(s) 478
TaiI ACGT 4 cut(s) 73, 196, 269, 503
TaqI TCGA 1 cut(s) 396
TauI GCSGC 1 cut(s) 118
TfiI GAWTC 2 cut(s) 102, 185
Tru1I TTAA 1 cut(s) 8
Tru9I TTAA 1 cut(s) 8
TseFI GTSAC 1 cut(s) 57
Tsp45I GTSAC 1 cut(s) 57
TspGWI ACGGA 1 cut(s) 65
VpaK11BI GGWCC 1 cut(s) 23
XhoI CTCGAG 1 cut(s) 395
XspI CTAG 2 cut(s) 296, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.