FvH4_5g32301

LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0028017
Physical Location & Seq
Reverse (-)
23305794 .. 23307550
1757 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32301.t1

Sequence Viewer

Length: 1452 bp
ATGGAAAATGTGGGAACTACTGCAAGCTGCCAAACTCGAGGAGCAGCTTGTAAGGAGTTTATAGACAGATTTAGTGATCTTCCAGATGAAATTGCTCATCAAATTCTTTCCTTACTGAATTTAAGAGACCTTACTCGGGTGAGCTGTGTGTCCAAAAGATGTACAAAGCTTTATCTTTCACTTCCGTCATTGAATTTTGATACAACCAGTAGCGTATCAATGTGGAAGCGGCTGCAGGTGTTCAACAGTTCTGATAGGTTCCTGAGTAGTCTTGACGATAATACAATTCAATACTTTCGTATTCGTTGGACTTACTATCCTGTCAAAAATTCCAGCCACTTTAATGAGCTTTATAGGATATTCACATGGATCCGAAATGCAATAAGGGGCAATGTTGAAGTGTTTGATCTTCAGCTCGATACATTACAGAAAACACCTTTAGTATTACCATCTTGGTTCTTCCATTGTTCATCTTTGAAATATTTACTTTTCGATTTTGGAGGGGACATTCTTAAATGGCCATCCTCTTCTGCTTGTTTCCCTAATCTCCAAGTCTTGAAGTTCAGAAAGTTCAAAATTCAAGGAGAGGAATATTTTTTCAAATGGATCTTATGTTCCCGCAAAAGCCTGAAGAAATTACATCTTGAAGAAGTTCGCGGGATAAAATTTATGACGATTGAAAGCTTGTCTTTGGAATCATTTTGTTTTGTGCATTATTTTCAGAGTGATTTGTGCCATCTTAAGGTATCAGGTCAGAAACTAGAGAATATACATATATCATGGGAGTTTGATTCGAGTAGCAGGAGATCGTTGCATATCTGTACTCCAAACCTTAAATATTTGAAATGGAGTGGTAGTTTGATGGATAATACGGATCTGGGGGATCTAATGTGCCTAGAAAAAGCTGAGATTTTTCTAGAGCCTCTGAAAGAAGACTATGTCAAAGTGTTTGAGACTCTTGACGGTATCAGGATGCTTAAAGCTCTTTCCCTAAACCAAAAGACTACAAAGGCTCTATTCAGGGAACATGCCGTCCAAGCACTTTTTGAAAATATTTCTCACTTGCAGATCAGTATTACGAGCTTGAGTGATGACTTAATTCCTTCAATGGTCTCTCTTCCCAGAGGAATGACAACTTTGAGTACTTTCTACATACACTCTGATCCATCCTTCATAGGCTACCCTGTACATGAAAATAGTGTATCCACATATAGTATGAGTTACTGGATATCCCAAAACCTTTCCTGTATTTGTCGGCTTGAGGAGGTGAGATTAGAGCTTTCTGATGCCAATGAAAGGGAATTGGCATGTTATATACTTGAGCATGCTAAAAATTTGAAGAAAATGGTCATTCTTTATTCACCATCTCAAGCACCTGGTGCATGGTTCTTAAGAATAAGAAGAATTGTTTCTACTGCGACGGTTGTCTTCAAGAATAGATATAGCAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBD

Protein Analysis

484

Amino Acids

56.1

Weight (kDa)

8.62

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 24 - 53 1.1e-07 F-box-like
F-box PF00646 24 - 54 3.7e-07 F-box domain
LRR_At5g56370 PF24758 122 - 293 6.6e-09 FBD-associated F-box protein At5g56370, LRR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028017)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g32301
prunus_persica Prupe.1G551800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccII CGCG 1 cut(s) 657
AciI CCGC 3 cut(s) 229, 619, 657
AclWI GGATC 6 cut(s) 364, 377, 614, 882, 891, 1157
AcoI YGGCCR 1 cut(s) 518
AcsI RAATTY 7 cut(s) 102, 118, 193, 328, 576, 665, 1333
AcuI CTGAAG 2 cut(s) 395, 650
AdeI CACNNNGTG 1 cut(s) 1379
AfaI GTAC 4 cut(s) 163, 823, 1144, 1188
AfiI CCNNNNNNNGG 3 cut(s) 136, 742, 1296
AflII CTTAAG 2 cut(s) 740, 1390
AjnI CCWGG 1 cut(s) 1375
AloI GAACNNNNNNTCC 4 cut(s) 598, 630, 1017, 1049
Alw26I GTCTC 3 cut(s) 120, 947, 1117
AlwI GGATC 6 cut(s) 364, 377, 614, 882, 891, 1157
Ama87I CYCGRG 2 cut(s) 36, 135
AoxI GGCC 1 cut(s) 518
ApeKI GCWGC 3 cut(s) 27, 44, 232
ApoI RAATTY 7 cut(s) 102, 118, 193, 328, 576, 665, 1333
Asp700I GAANNNNTTC 2 cut(s) 651, 1408
AsuHPI GGTGA 3 cut(s) 151, 1279, 1353
AvaI CYCGRG 2 cut(s) 36, 135
BalI TGGCCA 1 cut(s) 520
BamHI GGATCC 1 cut(s) 369
BbsI GAAGAC 2 cut(s) 939, 1420
BbvI GCAGC 3 cut(s) 14, 56, 219
BccI CCATC 6 cut(s) 457, 529, 744, 856, 1174, 1372
BceAI ACGGC 1 cut(s) 1016
BciT130I CCWGG 1 cut(s) 1377
BciVI GTATCC 1 cut(s) 1213
BcoDI GTCTC 3 cut(s) 120, 947, 1117
BfaI CTAG 3 cut(s) 761, 896, 917
BfmI CTRYAG 1 cut(s) 233
BfrI CTTAAG 2 cut(s) 740, 1390
BfuAI ACCTGC 1 cut(s) 226
BfuI GTATCC 1 cut(s) 1213
BisI GCNGC 4 cut(s) 28, 45, 230, 233
BlsI GCNGC 4 cut(s) 29, 46, 231, 234
BmcAI AGTACT 1 cut(s) 1144
Bme1390I CCNGG 1 cut(s) 1377
BmeT110I CYCGRG 2 cut(s) 36, 135
BmiI GGNNCC 2 cut(s) 260, 371
BmrFI CCNGG 1 cut(s) 1377
BmsI GCATC 2 cut(s) 963, 1276
BoxI GACNNNNGTC 1 cut(s) 1424
BpiI GAAGAC 2 cut(s) 939, 1420
BpuEI CTTGAG 4 cut(s) 1105, 1280, 1340, 1353
BsaI GGTCTC 2 cut(s) 120, 1117
BsaXI ACNNNNNCTCC 2 cut(s) 841, 871
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 742, 1296
Bse1I ACTGG 2 cut(s) 207, 1229
Bse3DI GCAATG 1 cut(s) 397
BseBI CCWGG 1 cut(s) 1377
BseGI GGATG 3 cut(s) 521, 978, 1166
BseLI CCNNNNNNNGG 3 cut(s) 136, 742, 1296
BseMI GCAATG 1 cut(s) 397
BseMII CTCAG 2 cut(s) 254, 897
BseNI ACTGG 2 cut(s) 207, 1229
BseRI GAGGAG 2 cut(s) 54, 1277
BseXI GCAGC 3 cut(s) 14, 56, 219
Bsh1236I CGCG 1 cut(s) 657
BshFI GGCC 1 cut(s) 520
BsiHKCI CYCGRG 2 cut(s) 36, 135
BslFI GGGAC 1 cut(s) 518
BslI CCNNNNNNNGG 3 cut(s) 136, 742, 1296
BsmAI GTCTC 3 cut(s) 120, 947, 1117
BsmFI GGGAC 1 cut(s) 518
BsnI GGCC 1 cut(s) 520
Bso31I GGTCTC 2 cut(s) 120, 1117
BsoBI CYCGRG 2 cut(s) 36, 135
Bsp1407I TGTACA 2 cut(s) 161, 1186
Bsp143I GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
BspACI CCGC 3 cut(s) 229, 619, 657
BspANI GGCC 1 cut(s) 520
BspCNI CTCAG 2 cut(s) 255, 898
BspFNI CGCG 1 cut(s) 657
BspLI GGNNCC 2 cut(s) 260, 371
BspMAI CTGCAG 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 6 cut(s) 364, 377, 614, 882, 891, 1157
BspTI CTTAAG 2 cut(s) 740, 1390
BspTNI GGTCTC 2 cut(s) 120, 1117
BsrDI GCAATG 1 cut(s) 397
BsrGI TGTACA 2 cut(s) 161, 1186
BsrI ACTGG 2 cut(s) 207, 1229
BssMI GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
Bst2UI CCWGG 1 cut(s) 1377
Bst4CI ACNGT 3 cut(s) 248, 965, 1423
Bst6I CTCTTC 2 cut(s) 532, 1122
BstAFI CTTAAG 2 cut(s) 740, 1390
BstAPI GCANNNNNTGC 1 cut(s) 1379
BstAUI TGTACA 2 cut(s) 161, 1186
BstC8I GCNNGC 2 cut(s) 25, 1326
BstDEI CTNAG 2 cut(s) 263, 906
BstF5I GGATG 3 cut(s) 521, 978, 1166
BstFNI CGCG 1 cut(s) 657
BstKTI GATC 9 cut(s) 79, 372, 409, 609, 809, 877, 886, 1071, 1165
BstMAI GTCTC 3 cut(s) 120, 947, 1117
BstMBI GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
BstMWI GCNNNNNNNGC 2 cut(s) 1037, 1379
BstNI CCWGG 1 cut(s) 1377
BstNSI RCATGY 3 cut(s) 1031, 1311, 1328
BstPAI GACNNNNGTC 1 cut(s) 1424
BstSCI CCNGG 1 cut(s) 1375
BstSFI CTRYAG 1 cut(s) 233
BstUI CGCG 1 cut(s) 657
BstV1I GCAGC 3 cut(s) 14, 56, 219
BstV2I GAAGAC 2 cut(s) 939, 1420
BstX2I RGATCY 4 cut(s) 369, 606, 874, 883
BstYI RGATCY 4 cut(s) 369, 606, 874, 883
BsuI GTATCC 1 cut(s) 1213
BsuRI GGCC 1 cut(s) 520
BtsCI GGATG 3 cut(s) 521, 978, 1166
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 25, 1326
CsiI ACCWGGT 1 cut(s) 1375
Csp6I GTAC 4 cut(s) 162, 822, 1143, 1187
CviAII CATG 7 cut(s) 366, 780, 1028, 1190, 1308, 1325, 1383
CviQI GTAC 4 cut(s) 162, 822, 1143, 1187
DdeI CTNAG 2 cut(s) 263, 906
DpnI GATC 9 cut(s) 78, 371, 408, 608, 808, 876, 885, 1070, 1164
DpnII GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
DraIII CACNNNGTG 1 cut(s) 1379
EaeI YGGCCR 1 cut(s) 518
Eam1104I CTCTTC 2 cut(s) 532, 1122
EarI CTCTTC 2 cut(s) 532, 1122
Eco31I GGTCTC 2 cut(s) 120, 1117
Eco32I GATATC 1 cut(s) 1230
Eco57I CTGAAG 2 cut(s) 395, 650
Eco88I CYCGRG 2 cut(s) 36, 135
EcoRII CCWGG 1 cut(s) 1375
EcoRV GATATC 1 cut(s) 1230
FaeI CATG 7 cut(s) 369, 783, 1031, 1193, 1311, 1328, 1386
FalI AAGNNNNNCTT 2 cut(s) 673, 705
FaqI GGGAC 1 cut(s) 518
FatI CATG 7 cut(s) 365, 779, 1027, 1189, 1307, 1324, 1382
FauI CCCGC 2 cut(s) 626, 650
Fnu4HI GCNGC 4 cut(s) 28, 45, 230, 233
FokI GGATG 3 cut(s) 508, 985, 1153
Fsp4HI GCNGC 4 cut(s) 28, 45, 230, 233
FspBI CTAG 3 cut(s) 761, 896, 917
GluI GCNGC 4 cut(s) 28, 45, 230, 233
HaeIII GGCC 1 cut(s) 520
Hin1II CATG 7 cut(s) 369, 783, 1031, 1193, 1311, 1328, 1386
HindIII AAGCTT 2 cut(s) 167, 682
HinfI GANTC 3 cut(s) 695, 791, 955
HphI GGTGA 3 cut(s) 151, 1279, 1353
Hpy188I TCNGA 8 cut(s) 253, 374, 566, 723, 756, 927, 1162, 1285
Hpy188III TCNNGA 9 cut(s) 83, 262, 272, 556, 644, 917, 959, 970, 1432
Hpy99I CGWCG 1 cut(s) 1423
HpyAV CCTTC 2 cut(s) 1113, 1180
HpyCH4III ACNGT 3 cut(s) 248, 965, 1423
HpyCH4V TGCA 7 cut(s) 23, 235, 380, 712, 814, 1066, 1382
HpyF10VI GCNNNNNNNGC 2 cut(s) 1037, 1379
HpyF3I CTNAG 2 cut(s) 263, 906
Hsp92II CATG 7 cut(s) 369, 783, 1031, 1193, 1311, 1328, 1386
Kzo9I GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 3 cut(s) 14, 56, 219
LweI GCATC 2 cut(s) 963, 1276
MabI ACCWGGT 1 cut(s) 1375
MaeI CTAG 3 cut(s) 761, 896, 917
MaeIII GTNAC 1 cut(s) 1220
MalI GATC 9 cut(s) 78, 371, 408, 608, 808, 876, 885, 1070, 1164
MboI GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
MflI RGATCY 4 cut(s) 369, 606, 874, 883
MlsI TGGCCA 1 cut(s) 520
MluNI TGGCCA 1 cut(s) 520
MlyI GAGTC 1 cut(s) 949
MmeI TCCRAC 1 cut(s) 287
MnlI CCTC 8 cut(s) 32, 494, 535, 580, 933, 1118, 1255, 1258
Mox20I TGGCCA 1 cut(s) 520
MroXI GAANNNNTTC 2 cut(s) 651, 1408
MscI TGGCCA 1 cut(s) 520
MseI TTAA 8 cut(s) 122, 342, 513, 741, 834, 978, 1097, 1391
MslI CAYNNNNRTG 1 cut(s) 342
Msp20I TGGCCA 1 cut(s) 520
MspCI CTTAAG 2 cut(s) 740, 1390
MspR9I CCNGG 1 cut(s) 1377
MvaI CCWGG 1 cut(s) 1377
MvnI CGCG 1 cut(s) 657
MwoI GCNNNNNNNGC 2 cut(s) 1037, 1379
NdeII GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
NlaIII CATG 7 cut(s) 369, 783, 1031, 1193, 1311, 1328, 1386
NlaIV GGNNCC 2 cut(s) 260, 371
NspI RCATGY 3 cut(s) 1031, 1311, 1328
PaeI GCATGC 1 cut(s) 1328
PaeR7I CTCGAG 1 cut(s) 36
PaqCI CACCTGC 1 cut(s) 226
PdmI GAANNNNTTC 2 cut(s) 651, 1408
PfeI GAWTC 2 cut(s) 695, 791
PflFI GACNNNGTC 1 cut(s) 938
PkrI GCNGC 4 cut(s) 29, 46, 231, 234
PleI GAGTC 1 cut(s) 949
PpsI GAGTC 1 cut(s) 949
PshAI GACNNNNGTC 1 cut(s) 1424
Psp6I CCWGG 1 cut(s) 1375
PspGI CCWGG 1 cut(s) 1375
PspN4I GGNNCC 2 cut(s) 260, 371
PspXI VCTCGAGB 1 cut(s) 36
PstI CTGCAG 1 cut(s) 237
PsuI RGATCY 4 cut(s) 369, 606, 874, 883
PsyI GACNNNGTC 1 cut(s) 938
RsaI GTAC 4 cut(s) 163, 823, 1144, 1188
RsaNI GTAC 4 cut(s) 162, 822, 1143, 1187
RseI CAYNNNNRTG 1 cut(s) 342
SaqAI TTAA 8 cut(s) 122, 342, 513, 741, 834, 978, 1097, 1391
SatI GCNGC 4 cut(s) 28, 45, 230, 233
Sau3AI GATC 9 cut(s) 76, 369, 406, 606, 806, 874, 883, 1068, 1162
ScaI AGTACT 1 cut(s) 1144
SchI GAGTC 1 cut(s) 949
ScrFI CCNGG 1 cut(s) 1377
SexAI ACCWGGT 1 cut(s) 1375
SfaNI GCATC 2 cut(s) 963, 1276
SfcI CTRYAG 1 cut(s) 233
Sfr274I CTCGAG 1 cut(s) 36
SlaI CTCGAG 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 342
SmlI CTYRAG 7 cut(s) 36, 740, 1084, 1259, 1319, 1368, 1390
SmoI CTYRAG 7 cut(s) 36, 740, 1084, 1259, 1319, 1368, 1390
SphI GCATGC 1 cut(s) 1328
SsiI CCGC 3 cut(s) 229, 619, 657
SspI AATATT 4 cut(s) 482, 593, 839, 1054
SspMI CTAG 3 cut(s) 761, 896, 917
StyD4I CCNGG 1 cut(s) 1375
TaaI ACNGT 3 cut(s) 248, 965, 1423
TaqI TCGA 4 cut(s) 37, 417, 492, 794
TatI WGTACW 4 cut(s) 161, 821, 1142, 1186
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 2 cut(s) 695, 791
Tru1I TTAA 8 cut(s) 122, 342, 513, 741, 834, 978, 1097, 1391
Tru9I TTAA 8 cut(s) 122, 342, 513, 741, 834, 978, 1097, 1391
TseI GCWGC 3 cut(s) 27, 44, 232
TspDTI ATGAA 5 cut(s) 102, 459, 1162, 1206, 1308
TspGWI ACGGA 2 cut(s) 174, 887
Tth111I GACNNNGTC 1 cut(s) 938
Vha464I CTTAAG 2 cut(s) 740, 1390
XapI RAATTY 7 cut(s) 102, 118, 193, 328, 576, 665, 1333
XbaI TCTAGA 1 cut(s) 916
XceI RCATGY 3 cut(s) 1031, 1311, 1328
XhoI CTCGAG 1 cut(s) 36
XmnI GAANNNNTTC 2 cut(s) 651, 1408
XspI CTAG 3 cut(s) 761, 896, 917
ZrmI AGTACT 1 cut(s) 1144
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.