FvH4_6g04270

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0020332
Physical Location & Seq
Forward (+)
2326936 .. 2329803
2868 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04270.t3

Sequence Viewer

Length: 786 bp
ATGCAAGAAGAAGCGTCGTCAACCTTGCTTCGGTTGACGAAACGCTTGAAGGACATGGAGGGGAAATTTCGGTCCACCCGGATCGCCTTCCTTGCCCGATGTGAAGAGGTGGAGGAACGTGAGAGAAAGCTGGCGGCGTTGAAGGCCGAGGCAGAGAACAAAGAGGCGGAGCTCCGAGACCTCGAGAGGCTGATCGAGGAGAAGAAGCGTGATACTTTATTAGAGAAGGAGCAGTTAAAACATGCCACTGCTCGAGATTTGCAGCTTATGATGAGTTACATGTTGATGAGGCGCGATCTCGTTGTAAATGAAGTCTCAGCTGCTTTGCGAGCGTCAGAAGATCCAGCACAGCTGGTTTTGGATGCAATGAAAGAATTTTACCCTTCAGATGATTCTGTTGGGGATGGAGATAGGGCTGAGTTTGATACGGTCGTCGTCTACCGAAGGAGTTGCGTTGCTTTGTTAGAGGAGTTGAAGAAGATGAAGATCACAAGGGAAACGAAGACTAGGGTGAGAGAAGAAGCGATGAAAATGGCGAGTGATTGGAAGGGGAAGATGAAGGTAGCGGATGAGAATTGGTTGGAGGCTTTGGGATTCTTGCTTCTTGTGACTACATATGGCTTGACCTATGCGTTTGATACGACGGAGCTTCAAGATCTCCTTATTATAGTTGCTCAACAGGAGCAGGCAACTGAGTTATGCCGAGACCTAGGCATCATATTTAAGCAGGCACCAGCCCACTGCCACCAGTACCGGTGCAAGGCATTTGCAGCGGTTTCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

262

Amino Acids

30.11

Weight (kDa)

5.69

Isoelectric Point (pI)

50.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 97 - 240 2.8e-33 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020332)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04270 FvH4_6g04270 FvH4_6g04270
rosa_multiflora Rmu_sc0005888.1_g000008
rosa_rugosa Rorug02G0644400.1 Rorug02G0646100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 438
AccII CGCG 1 cut(s) 294
AciI CCGC 4 cut(s) 134, 167, 566, 773
AclWI GGATC 2 cut(s) 89, 335
AcsI RAATTY 2 cut(s) 65, 374
AcuI CTGAAG 1 cut(s) 369
AfaI GTAC 1 cut(s) 752
AfiI CCNNNNNNNGG 2 cut(s) 30, 760
AflIII ACRYGT 1 cut(s) 279
AgeI ACCGGT 1 cut(s) 753
AgsI TTSAA 4 cut(s) 49, 142, 475, 653
AluBI AGCT 6 cut(s) 130, 172, 265, 320, 352, 649
AluI AGCT 6 cut(s) 130, 172, 265, 320, 352, 649
Alw21I GWGCWC 1 cut(s) 174
Alw26I GTCTC 3 cut(s) 171, 319, 699
AlwI GGATC 2 cut(s) 89, 335
Ama87I CYCGRG 2 cut(s) 182, 252
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 3 cut(s) 262, 320, 770
ApoI RAATTY 2 cut(s) 65, 374
AsiGI ACCGGT 1 cut(s) 753
AspA2I CCTAGG 1 cut(s) 709
AspLEI GCGC 1 cut(s) 294
AspS9I GGNCC 1 cut(s) 72
AsuC2I CCSGG 1 cut(s) 79
AsuHPI GGTGA 1 cut(s) 523
AvaI CYCGRG 2 cut(s) 182, 252
AvaII GGWCC 1 cut(s) 72
AvrII CCTAGG 1 cut(s) 709
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 174
BbsI GAAGAC 1 cut(s) 509
Bbv12I GWGCWC 1 cut(s) 174
BbvI GCAGC 3 cut(s) 274, 307, 782
BccI CCATC 1 cut(s) 398
BcgI CGANNNNNNTGC 2 cut(s) 432, 466
BcnI CCSGG 1 cut(s) 79
BcoDI GTCTC 3 cut(s) 171, 319, 699
BfaI CTAG 2 cut(s) 507, 710
BglII AGATCT 1 cut(s) 655
BisI GCNGC 4 cut(s) 135, 263, 321, 771
BlnI CCTAGG 1 cut(s) 709
BlsI GCNGC 4 cut(s) 136, 264, 322, 772
Bme1390I CCNGG 1 cut(s) 79
Bme18I GGWCC 1 cut(s) 72
BmeT110I CYCGRG 2 cut(s) 182, 252
BmgT120I GGNCC 1 cut(s) 72
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 1 cut(s) 79
BmsI GCATC 2 cut(s) 352, 723
BpiI GAAGAC 1 cut(s) 509
BpuMI CCSGG 1 cut(s) 79
BsaBI GATNNNNATC 1 cut(s) 485
BsaI GGTCTC 2 cut(s) 171, 699
BsaJI CCNNGG 2 cut(s) 147, 709
BsaWI WCCGGW 1 cut(s) 753
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 760
Bse118I RCCGGY 1 cut(s) 753
Bse1I ACTGG 1 cut(s) 748
Bse3DI GCAATG 1 cut(s) 372
Bse8I GATNNNNATC 1 cut(s) 485
BseDI CCNNGG 2 cut(s) 147, 709
BseGI GGATG 3 cut(s) 367, 409, 574
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 2 cut(s) 30, 760
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 3 cut(s) 330, 408, 684
BseNI ACTGG 1 cut(s) 748
BseRI GAGGAG 2 cut(s) 212, 482
BseXI GCAGC 3 cut(s) 274, 307, 782
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 1 cut(s) 730
BshTI ACCGGT 1 cut(s) 753
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 174
BsiHKCI CYCGRG 2 cut(s) 182, 252
BsiSI CCGG 2 cut(s) 79, 754
BslI CCNNNNNNNGG 2 cut(s) 30, 760
BsmAI GTCTC 3 cut(s) 171, 319, 699
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 2 cut(s) 171, 699
BsoBI CYCGRG 2 cut(s) 182, 252
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 6 cut(s) 81, 192, 295, 340, 486, 655
BspACI CCGC 4 cut(s) 134, 167, 566, 773
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 3 cut(s) 329, 409, 685
BspFNI CGCG 1 cut(s) 294
BspLI GGNNCC 1 cut(s) 732
BspPI GGATC 2 cut(s) 89, 335
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 2 cut(s) 171, 699
BsrDI GCAATG 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 753
BsrI ACTGG 1 cut(s) 748
BssAI RCCGGY 1 cut(s) 753
BssECI CCNNGG 2 cut(s) 147, 709
BssMI GATC 6 cut(s) 81, 192, 295, 340, 486, 655
BssT1I CCWWGG 1 cut(s) 709
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 99
BstC8I GCNNGC 4 cut(s) 132, 330, 687, 729
BstDEI CTNAG 3 cut(s) 316, 417, 693
BstF5I GGATG 3 cut(s) 367, 409, 574
BstFNI CGCG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 294
BstKTI GATC 6 cut(s) 84, 195, 298, 343, 489, 658
BstMAI GTCTC 3 cut(s) 171, 319, 699
BstMBI GATC 6 cut(s) 81, 192, 295, 340, 486, 655
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 4 cut(s) 92, 143, 329, 770
BstNSI RCATGY 2 cut(s) 245, 283
BstSCI CCNGG 1 cut(s) 77
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 274, 307, 782
BstV2I GAAGAC 1 cut(s) 509
BstX2I RGATCY 2 cut(s) 340, 655
BstYI RGATCY 2 cut(s) 340, 655
BsuRI GGCC 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 539
BtsCI GGATG 3 cut(s) 367, 409, 574
BtsI GCAGTG 2 cut(s) 246, 739
BtsIMutI CAGTG 2 cut(s) 246, 739
Cac8I GCNNGC 4 cut(s) 132, 330, 687, 729
CfoI GCGC 1 cut(s) 294
Cfr10I RCCGGY 1 cut(s) 753
Cfr13I GGNCC 1 cut(s) 72
CseI GACGC 2 cut(s) 3, 321
Csp6I GTAC 1 cut(s) 751
CspAI ACCGGT 1 cut(s) 753
CviAII CATG 3 cut(s) 55, 242, 280
CviQI GTAC 1 cut(s) 751
DdeI CTNAG 3 cut(s) 316, 417, 693
DpnI GATC 6 cut(s) 83, 194, 297, 342, 488, 657
DpnII GATC 6 cut(s) 81, 192, 295, 340, 486, 655
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
EciI GGCGGA 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 174
Eco31I GGTCTC 2 cut(s) 171, 699
Eco47I GGWCC 1 cut(s) 72
Eco53kI GAGCTC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 369
Eco88I CYCGRG 2 cut(s) 182, 252
EcoICRI GAGCTC 1 cut(s) 172
EcoT14I CCWWGG 1 cut(s) 709
EcoT38I GRGCYC 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 709
FaeI CATG 3 cut(s) 58, 245, 283
FalI AAGNNNNNCTT 2 cut(s) 645, 677
FatI CATG 3 cut(s) 54, 241, 279
FauNDI CATATG 1 cut(s) 616
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 4 cut(s) 135, 263, 321, 771
FokI GGATG 3 cut(s) 374, 416, 581
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 4 cut(s) 135, 263, 321, 771
FspBI CTAG 2 cut(s) 507, 710
GlaI GCGC 1 cut(s) 293
GluI GCNGC 4 cut(s) 135, 263, 321, 771
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 79, 754
HgaI GACGC 2 cut(s) 3, 321
HhaI GCGC 1 cut(s) 294
Hin1II CATG 3 cut(s) 58, 245, 283
Hin6I GCGC 1 cut(s) 292
HinP1I GCGC 1 cut(s) 292
HincII GTYRAC 2 cut(s) 21, 36
HindII GTYRAC 2 cut(s) 21, 36
HinfI GANTC 2 cut(s) 392, 594
HpaII CCGG 2 cut(s) 79, 754
HphI GGTGA 1 cut(s) 523
Hpy166II GTNNAC 4 cut(s) 21, 36, 75, 439
Hpy188I TCNGA 3 cut(s) 176, 337, 388
Hpy188III TCNNGA 3 cut(s) 184, 254, 653
Hpy8I GTNNAC 4 cut(s) 21, 36, 75, 439
Hpy99I CGWCG 3 cut(s) 19, 437, 646
HpyAV CCTTC 8 cut(s) 43, 97, 136, 220, 393, 438, 541, 553
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 5 cut(s) 4, 262, 365, 759, 770
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 143, 329, 770
HpyF3I CTNAG 3 cut(s) 316, 417, 693
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 3 cut(s) 58, 245, 283
HspAI GCGC 1 cut(s) 292
Kzo9I GATC 6 cut(s) 81, 192, 295, 340, 486, 655
LmnI GCTCC 5 cut(s) 169, 177, 229, 646, 682
Lsp1109I GCAGC 3 cut(s) 274, 307, 782
LweI GCATC 2 cut(s) 352, 723
MaeI CTAG 2 cut(s) 507, 710
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 2 cut(s) 275, 607
MalI GATC 6 cut(s) 83, 194, 297, 342, 488, 657
MboI GATC 6 cut(s) 81, 192, 295, 340, 486, 655
MflI RGATCY 2 cut(s) 340, 655
MhlI GDGCHC 1 cut(s) 174
MluCI AATT 3 cut(s) 65, 374, 574
MmeI TCCRAC 1 cut(s) 561
MseI TTAA 2 cut(s) 236, 723
MslI CAYNNNNRTG 1 cut(s) 284
MspA1I CMGCKG 3 cut(s) 320, 352, 773
MspI CCGG 2 cut(s) 79, 754
MspR9I CCNGG 1 cut(s) 79
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 4 cut(s) 92, 143, 329, 770
NciI CCSGG 1 cut(s) 79
NdeI CATATG 1 cut(s) 616
NdeII GATC 6 cut(s) 81, 192, 295, 340, 486, 655
NlaIII CATG 3 cut(s) 58, 245, 283
NlaIV GGNNCC 1 cut(s) 732
NmeAIII GCCGAG 2 cut(s) 172, 728
NmuCI GTSAC 1 cut(s) 607
NspI RCATGY 2 cut(s) 245, 283
PaeR7I CTCGAG 2 cut(s) 182, 252
PciI ACATGT 1 cut(s) 279
PfeI GAWTC 2 cut(s) 392, 594
PinAI ACCGGT 1 cut(s) 753
PkrI GCNGC 4 cut(s) 136, 264, 322, 772
PscI ACATGT 1 cut(s) 279
Psp124BI GAGCTC 1 cut(s) 174
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 1 cut(s) 72
PsuI RGATCY 2 cut(s) 340, 655
PvuII CAGCTG 2 cut(s) 320, 352
RsaI GTAC 1 cut(s) 752
RsaNI GTAC 1 cut(s) 751
RseI CAYNNNNRTG 1 cut(s) 284
SacI GAGCTC 1 cut(s) 174
SaqAI TTAA 2 cut(s) 236, 723
SatI GCNGC 4 cut(s) 135, 263, 321, 771
Sau3AI GATC 6 cut(s) 81, 192, 295, 340, 486, 655
Sau96I GGNCC 1 cut(s) 72
ScrFI CCNGG 1 cut(s) 79
SduI GDGCHC 1 cut(s) 174
SfaNI GCATC 2 cut(s) 352, 723
Sfr274I CTCGAG 2 cut(s) 182, 252
SinI GGWCC 1 cut(s) 72
SlaI CTCGAG 2 cut(s) 182, 252
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 182, 252
SmoI CTYRAG 2 cut(s) 182, 252
Sse9I AATT 3 cut(s) 65, 374, 574
SsiI CCGC 4 cut(s) 134, 167, 566, 773
SspMI CTAG 2 cut(s) 507, 710
SstI GAGCTC 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 77
StyI CCWWGG 1 cut(s) 709
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 183, 195, 253
TaqII GACCGA 1 cut(s) 60
TasI AATT 3 cut(s) 65, 374, 574
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 392, 594
Tru1I TTAA 2 cut(s) 236, 723
Tru9I TTAA 2 cut(s) 236, 723
TscAI CASTG 2 cut(s) 253, 746
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 3 cut(s) 262, 320, 770
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 5 cut(s) 324, 383, 497, 542, 572
TspGWI ACGGA 1 cut(s) 659
TspRI CASTG 2 cut(s) 253, 746
VpaK11BI GGWCC 1 cut(s) 72
XapI RAATTY 2 cut(s) 65, 374
XceI RCATGY 2 cut(s) 245, 283
XhoI CTCGAG 2 cut(s) 182, 252
XmaJI CCTAGG 1 cut(s) 709
XmiI GTMKAC 1 cut(s) 438
XspI CTAG 2 cut(s) 507, 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.