Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0015148

Physical Location & Seq

Genomic Coordinates Forward (+)

24844440 .. 24847868

Gene Structure 3429 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g31730.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1620 bp

ATGGTTCGGGCTATTGAGGACAATCCAGAGAATCACAAGACCAGGTTGCACCATCTCAGGGAGATGCATGAGACCGCAGGCAGGCATACCAAGAGCTTGAGCCTTGAGGCTTCAAGAGCATTAGAGTTTCAGGATGACGATGGTGGGGATGAAACTCAGACGCCTACTCAGTCCAACCCTAGAGGCCTCAAGTCAAGGCTGACCATGGAGGAAGCCACCGCGGCTGCCAAAGAAGCCAAAGAAAAGCAGCAGCAATCTGATATAGAACAGATGAAGGAGAGGTTTGCTAAATTGCTTTTGGGTGAAGATATGTCCGGCGGAGGAAAGGGTGTTTCTTCAGCTTTGGCATTGTCCAATGCCATTACAAACCTGGCCGCTTCTGTTTTTGGAGAGCAAACTCGCTTAGAGCCTATGTCTCCGGACAGGAAAGCAAGATGGAGAAAAGAAATAGATTGGTTGCTATGTGTCACCGATTACATTGTTGAGTTTGTTGCTTCACAACAGAAGAATAAGGATGGATCCAATATGGAGATAATGGTGACCCGGCAACGGACAGATCTCCTCATGAACATTCCTGCCCTGAAAAAGCTTGATGCAATGCTTATTGATTGTCTAGATAACTTCGAAGGCGATCAGGAATTCTTCTATGTATCTAAAGATGCTGATGAGAAGGAGAAAGGGAGTGGAAAGAGAAATGACAAATGGTGGCTACCAACTCCAAAAGTTCCACCAGATGGTCTATCAGAAGTGGCAAGAAAATTCTTGCAGTATCAAAAAGATTGTGTCAACCAAGTGCTTAAAGCAGCCATGGCCATAAATGCTCAAGTTTTAACAGAAATGGAGATTCCAGAAAACTACATTGAAGCCCTTCCCAAGAATGGGAGGGCAAGTCTTGGTGACCAAATCTATAGGAGTATTACAGTCGAATTCTTTGATCCGGACCAATTTCTTTGTGCCATGGACTTGTCCTCTGACCACAAAATTCTAGACTTAAAAAACAAAATGGAGGCTTCCATGGTCATATGGAAGAGAAAGATGAACCAAAAAGATGGAAAATCTGGCTGGGGCTCAGCTGTGAGTTTGGAAAAGAGAGAGCTCTTTGAAGAGAGAGCAGAGACCATCTTACTCCTTCTCAAGCAGAGGTTCCCTGGGATCCCTCAATCTTCATTGGACATAAGTAAAATTCAATACAATGGAGATGTCGGGCAGGCTATTCTCGAAAGCTATTCAAGAATCCTAGAGAGCTTGGCCTTCACTGTGTTGTCTCGAATTGAAGATGTACTCTATGCAGATTATTGTGTGCAGAATCCGTCAGCAGCAAGTAAAAAGAACAGTTTAGGAGAAACAACTCCCTCATCACCTTCAACTTCTCCGGATAGGTCCATGCATAAAGATGATAATGGGGGAACAGAGACACCCTCGTCAATGACACTATCGGATTTCATGGGTTGGGGATGTGATCAAGGTGATAGTGAGGCAAAGAAGGGACAGGAGGCTGCAGCAGATGAAAATGATGTCAAGCATCCACATATACAGAAACTAGCCAACATTGTGACCAACAAGAAGATGTCATATCTAGACACATTGGGTGGAATGAGAAGTCCAACGGCACGCCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000902 GO:0000904 GO:0001558 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009826 GO:0009856 GO:0009860 GO:0009932 GO:0009987 GO:0010154 GO:0010769 GO:0016020 GO:0016043 GO:0016049 GO:0017016 GO:0017048 GO:0019899 GO:0022414 GO:0022603 GO:0022604 GO:0030154 GO:0031267 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0040008 GO:0042802 GO:0042995 GO:0043900 GO:0044464 GO:0044706 GO:0045595 GO:0048316 GO:0048468 GO:0048588 GO:0048589 GO:0048608 GO:0048638 GO:0048731 GO:0048856 GO:0048868 GO:0048869 GO:0050789 GO:0050793 GO:0050794 GO:0051020 GO:0051128 GO:0051239 GO:0051510 GO:0051704 GO:0060284 GO:0060560 GO:0061458 GO:0065007 GO:0065009 GO:0071840 GO:0071944 GO:0080092 GO:0090406 GO:0098772 GO:0120025 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04962 map04962

Pfam Domains

Protein Families

PRONE

Protein Analysis

540

Amino Acids

60.35

Weight (kDa)

5.46

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRONE	PF03759	86 - 435	1.8e-172	PRONE (Plant-specific Rop nucleotide exchanger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0015148)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G79860
fragaria_vesca	FvH4_6g31730
malus_domestica	MD17G1191500.v1.1
prunus_persica	Prupe.3G067500_v2.0.a1
pyrus_communis	pycom09g12580 pycom17g19840
rosa_chinensis	RchiOBHm_Chr2g0138921
rosa_laevigata	RLG00000019768
rosa_multiflora	Rmu_sc0001376.1_g000001
rosa_roxburghii	Rroxscaffold_2G00105870
rosa_rugosa	Rorug02G0353100
rosa_samantha	Rh2AG403200 Rh2BG413700 Rh2CG389300 Rh2DG423500
rosa_wichuraiana	Rw2G032800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1420
AccB7I	CCANNNNNTGG	1 cut(s)	734
AccII	CGCG	1 cut(s)	221
AccIII	TCCGGA	3 cut(s)	418, 937, 1372
AciI	CCGC	5 cut(s)	75, 219, 221, 318, 375
AclWI	GGATC	5 cut(s)	513, 526, 929, 1147, 1160
AcoI	YGGCCR	2 cut(s)	372, 810
AcsI	RAATTY	5 cut(s)	638, 758, 926, 981, 1182
AcuI	CTGAAG	1 cut(s)	321
AcyI	GRCGYC	1 cut(s)	161
AfaI	GTAC	1 cut(s)	1281
AfiI	CCNNNNNNNGG	6 cut(s)	58, 81, 549, 734, 878, 879
AgsI	TTSAA	7 cut(s)	114, 863, 1103, 1187, 1230, 1274, 1365
AjnI	CCWGG	3 cut(s)	41, 369, 1147
AluBI	AGCT	7 cut(s)	96, 341, 589, 1073, 1096, 1224, 1245
AluI	AGCT	7 cut(s)	96, 341, 589, 1073, 1096, 1224, 1245
Alw21I	GWGCWC	1 cut(s)	1098
Alw26I	GTCTC	5 cut(s)	65, 420, 1109, 1269, 1406
AlwI	GGATC	5 cut(s)	513, 526, 929, 1147, 1160
Aor13HI	TCCGGA	3 cut(s)	418, 937, 1372
AoxI	GGCC	4 cut(s)	184, 372, 810, 1248
ApeKI	GCWGC	7 cut(s)	224, 247, 250, 803, 1316, 1496, 1499
ApoI	RAATTY	5 cut(s)	638, 758, 926, 981, 1182
Asp700I	GAANNNNTTC	1 cut(s)	867
AspS9I	GGNCC	2 cut(s)	940, 1380
AsuC2I	CCSGG	1 cut(s)	544
AsuHPI	GGTGA	6 cut(s)	314, 460, 550, 908, 1350, 1478
AsuII	TTCGAA	1 cut(s)	624
AvaII	GGWCC	2 cut(s)	940, 1380
BalI	TGGCCA	1 cut(s)	812
BamHI	GGATCC	2 cut(s)	518, 1152
BanII	GRGCYC	2 cut(s)	1070, 1098
Bbv12I	GWGCWC	1 cut(s)	1098
BbvI	GCAGC	7 cut(s)	211, 259, 262, 815, 1328, 1483, 1511
BccI	CCATC	7 cut(s)	60, 134, 429, 509, 728, 1043, 1127
BciT130I	CCWGG	3 cut(s)	43, 371, 1149
BclI	TGATCA	1 cut(s)	1459
BcnI	CCSGG	1 cut(s)	544
BcoDI	GTCTC	5 cut(s)	65, 420, 1109, 1269, 1406
BfaI	CTAG	6 cut(s)	180, 614, 986, 1238, 1541, 1577
BfmI	CTRYAG	2 cut(s)	907, 1497
BglI	GCCNNNNNGGC	1 cut(s)	221
BglII	AGATCT	1 cut(s)	556
BisI	GCNGC	9 cut(s)	222, 225, 248, 251, 375, 804, 1317, 1497, 1500
BlpI	GCTNAGC	1 cut(s)	1069
BlsI	GCNGC	9 cut(s)	223, 226, 249, 252, 376, 805, 1318, 1498, 1501
Bme1390I	CCNGG	4 cut(s)	43, 371, 544, 1149
Bme18I	GGWCC	2 cut(s)	940, 1380
BmgT120I	GGNCC	2 cut(s)	940, 1380
BmiI	GGNNCC	3 cut(s)	520, 1145, 1154
BmrFI	CCNGG	4 cut(s)	43, 371, 544, 1149
BmsI	GCATC	4 cut(s)	54, 583, 649, 1531
BplI	GAGNNNNNCTC	2 cut(s)	1403, 1435
Bpu1102I	GCTNAGC	1 cut(s)	1069
Bpu14I	TTCGAA	1 cut(s)	624
BpuEI	CTTGAG	5 cut(s)	118, 125, 173, 807, 1118
BpuMI	CCSGG	1 cut(s)	544
BsaHI	GRCGYC	1 cut(s)	161
BsaI	GGTCTC	2 cut(s)	65, 1109
BsaJI	CCNNGG	7 cut(s)	204, 219, 807, 957, 1014, 1147, 1148
BsaWI	WCCGGW	3 cut(s)	418, 937, 1372
BsaXI	ACNNNNNCTCC	2 cut(s)	874, 904
Bsc4I	CCNNNNNNNGG	6 cut(s)	58, 81, 549, 734, 878, 879
Bse3DI	GCAATG	1 cut(s)	603
BseAI	TCCGGA	3 cut(s)	418, 937, 1372
BseBI	CCWGG	3 cut(s)	43, 371, 1149
BseDI	CCNNGG	7 cut(s)	204, 219, 807, 957, 1014, 1147, 1148
BseGI	GGATG	5 cut(s)	139, 154, 520, 1460, 1522
BseLI	CCNNNNNNNGG	6 cut(s)	58, 81, 549, 734, 878, 879
BseMI	GCAATG	1 cut(s)	603
BseMII	CTCAG	4 cut(s)	70, 170, 182, 1083
BseRI	GAGGAG	1 cut(s)	551
BseXI	GCAGC	7 cut(s)	211, 259, 262, 815, 1328, 1483, 1511
BseYI	CCCAGC	1 cut(s)	1062
BsgI	GTGCAG	1 cut(s)	1322
Bsh1236I	CGCG	1 cut(s)	221
BshFI	GGCC	4 cut(s)	186, 374, 812, 1250
BsiHKAI	GWGCWC	1 cut(s)	1098
BsiSI	CCGG	5 cut(s)	315, 419, 544, 938, 1373
BslFI	GGGAC	1 cut(s)	1500
BslI	CCNNNNNNNGG	6 cut(s)	58, 81, 549, 734, 878, 879
BsmAI	GTCTC	5 cut(s)	65, 420, 1109, 1269, 1406
BsmFI	GGGAC	1 cut(s)	1500
BsnI	GGCC	4 cut(s)	186, 374, 812, 1250
Bso31I	GGTCTC	2 cut(s)	65, 1109
Bsp119I	TTCGAA	1 cut(s)	624
Bsp1286I	GDGCHC	2 cut(s)	1070, 1098
Bsp13I	TCCGGA	3 cut(s)	418, 937, 1372
Bsp143I	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
Bsp1720I	GCTNAGC	1 cut(s)	1069
Bsp19I	CCATGG	4 cut(s)	204, 807, 957, 1014
BspACI	CCGC	5 cut(s)	75, 219, 221, 318, 375
BspANI	GGCC	4 cut(s)	186, 374, 812, 1250
BspCNI	CTCAG	4 cut(s)	69, 169, 181, 1082
BspEI	TCCGGA	3 cut(s)	418, 937, 1372
BspFNI	CGCG	1 cut(s)	221
BspHI	TCATGA	1 cut(s)	564
BspLI	GGNNCC	3 cut(s)	520, 1145, 1154
BspMAI	CTGCAG	1 cut(s)	1501
BspPI	GGATC	5 cut(s)	513, 526, 929, 1147, 1160
BspT104I	TTCGAA	1 cut(s)	624
BspTNI	GGTCTC	2 cut(s)	65, 1109
BsrDI	GCAATG	1 cut(s)	603
BssECI	CCNNGG	7 cut(s)	204, 219, 807, 957, 1014, 1147, 1148
BssMI	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
BssNI	GRCGYC	1 cut(s)	161
BssT1I	CCWWGG	4 cut(s)	204, 807, 957, 1014
Bst2UI	CCWGG	3 cut(s)	43, 371, 1149
Bst4CI	ACNGT	3 cut(s)	922, 1258, 1334
Bst6I	CTCTTC	2 cut(s)	1022, 1098
BstACI	GRCGYC	1 cut(s)	161
BstBI	TTCGAA	1 cut(s)	624
BstC8I	GCNNGC	4 cut(s)	79, 83, 1209, 1612
BstDEI	CTNAG	5 cut(s)	56, 156, 168, 403, 1069
BstDSI	CCRYGG	5 cut(s)	204, 219, 807, 957, 1014
BstEII	GGTNACC	2 cut(s)	538, 896
BstF5I	GGATG	5 cut(s)	139, 154, 520, 1460, 1522
BstFNI	CGCG	1 cut(s)	221
BstKTI	GATC	6 cut(s)	521, 559, 634, 937, 1155, 1462
BstMAI	GTCTC	5 cut(s)	65, 420, 1109, 1269, 1406
BstMBI	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
BstMWI	GCNNNNNNNGC	5 cut(s)	116, 221, 233, 809, 818
BstNI	CCWGG	3 cut(s)	43, 371, 1149
BstPI	GGTNACC	2 cut(s)	538, 896
BstSCI	CCNGG	4 cut(s)	41, 369, 542, 1147
BstSFI	CTRYAG	2 cut(s)	907, 1497
BstUI	CGCG	1 cut(s)	221
BstV1I	GCAGC	7 cut(s)	211, 259, 262, 815, 1328, 1483, 1511
BstX2I	RGATCY	3 cut(s)	518, 556, 1152
BstXI	CCANNNNNNTGG	1 cut(s)	1049
BstYI	RGATCY	3 cut(s)	518, 556, 1152
BsuRI	GGCC	4 cut(s)	186, 374, 812, 1250
BtgI	CCRYGG	5 cut(s)	204, 219, 807, 957, 1014
BtsCI	GGATG	5 cut(s)	139, 154, 520, 1460, 1522
BtsIMutI	CAGTG	1 cut(s)	1254
Cac8I	GCNNGC	4 cut(s)	79, 83, 1209, 1612
CciI	TCATGA	1 cut(s)	564
Cfr13I	GGNCC	2 cut(s)	940, 1380
Cfr42I	CCGCGG	1 cut(s)	222
CseI	GACGC	1 cut(s)	169
CsiI	ACCWGGT	1 cut(s)	41
Csp6I	GTAC	1 cut(s)	1280
CviAII	CATG	8 cut(s)	68, 205, 565, 808, 958, 1015, 1384, 1444
CviQI	GTAC	1 cut(s)	1280
DdeI	CTNAG	5 cut(s)	56, 156, 168, 403, 1069
DpnI	GATC	6 cut(s)	520, 558, 633, 936, 1154, 1461
DpnII	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
DrdI	GACNNNNNNGTC	1 cut(s)	1420
DseDI	GACNNNNNNGTC	1 cut(s)	1420
EaeI	YGGCCR	2 cut(s)	372, 810
Eam1104I	CTCTTC	2 cut(s)	1022, 1098
EarI	CTCTTC	2 cut(s)	1022, 1098
EciI	GGCGGA	1 cut(s)	333
Ecl136II	GAGCTC	1 cut(s)	1096
Eco130I	CCWWGG	4 cut(s)	204, 807, 957, 1014
Eco147I	AGGCCT	1 cut(s)	186
Eco24I	GRGCYC	2 cut(s)	1070, 1098
Eco31I	GGTCTC	2 cut(s)	65, 1109
Eco47I	GGWCC	2 cut(s)	940, 1380
Eco53kI	GAGCTC	1 cut(s)	1096
Eco57I	CTGAAG	1 cut(s)	321
Eco91I	GGTNACC	2 cut(s)	538, 896
EcoICRI	GAGCTC	1 cut(s)	1096
EcoO65I	GGTNACC	2 cut(s)	538, 896
EcoRI	GAATTC	2 cut(s)	638, 926
EcoRII	CCWGG	3 cut(s)	41, 369, 1147
EcoT14I	CCWWGG	4 cut(s)	204, 807, 957, 1014
EcoT22I	ATGCAT	2 cut(s)	69, 1389
EcoT38I	GRGCYC	2 cut(s)	1070, 1098
ErhI	CCWWGG	4 cut(s)	204, 807, 957, 1014
FaeI	CATG	8 cut(s)	71, 208, 568, 811, 961, 1018, 1387, 1447
FaqI	GGGAC	1 cut(s)	1500
FatI	CATG	8 cut(s)	67, 204, 564, 807, 957, 1014, 1383, 1443
FauNDI	CATATG	1 cut(s)	1022
FbaI	TGATCA	1 cut(s)	1459
Fnu4HI	GCNGC	9 cut(s)	222, 225, 248, 251, 375, 804, 1317, 1497, 1500
FokI	GGATG	5 cut(s)	146, 161, 527, 1467, 1509
FriOI	GRGCYC	2 cut(s)	1070, 1098
Fsp4HI	GCNGC	9 cut(s)	222, 225, 248, 251, 375, 804, 1317, 1497, 1500
FspBI	CTAG	6 cut(s)	180, 614, 986, 1238, 1541, 1577
GluI	GCNGC	9 cut(s)	222, 225, 248, 251, 375, 804, 1317, 1497, 1500
GsaI	CCCAGC	1 cut(s)	1066
HaeIII	GGCC	4 cut(s)	186, 374, 812, 1250
HapII	CCGG	5 cut(s)	315, 419, 544, 938, 1373
HgaI	GACGC	1 cut(s)	169
Hin1I	GRCGYC	1 cut(s)	161
Hin1II	CATG	8 cut(s)	71, 208, 568, 811, 961, 1018, 1387, 1447
HincII	GTYRAC	1 cut(s)	787
HindII	GTYRAC	1 cut(s)	787
HindIII	AAGCTT	1 cut(s)	587
HinfI	GANTC	4 cut(s)	31, 844, 1233, 1306
HpaII	CCGG	5 cut(s)	315, 419, 544, 938, 1373
HphI	GGTGA	6 cut(s)	314, 460, 550, 908, 1350, 1478
Hpy166II	GTNNAC	1 cut(s)	787
Hpy188I	TCNGA	5 cut(s)	159, 259, 745, 973, 1438
Hpy8I	GTNNAC	1 cut(s)	787
HpyAV	CCTTC	8 cut(s)	268, 620, 664, 878, 1139, 1261, 1371, 1477
HpyCH4III	ACNGT	3 cut(s)	922, 1258, 1334
HpyCH4V	TGCA	8 cut(s)	49, 67, 596, 766, 1289, 1303, 1387, 1499
HpyF10VI	GCNNNNNNNGC	5 cut(s)	116, 221, 233, 809, 818
HpyF3I	CTNAG	5 cut(s)	56, 156, 168, 403, 1069
Hsp92I	GRCGYC	1 cut(s)	161
Hsp92II	CATG	8 cut(s)	71, 208, 568, 811, 961, 1018, 1387, 1447
Kpn2I	TCCGGA	3 cut(s)	418, 937, 1372
Ksp22I	TGATCA	1 cut(s)	1459
KspI	CCGCGG	1 cut(s)	222
Kzo9I	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
Lsp1109I	GCAGC	7 cut(s)	211, 259, 262, 815, 1328, 1483, 1511
LweI	GCATC	4 cut(s)	54, 583, 649, 1531
MabI	ACCWGGT	1 cut(s)	41
MaeI	CTAG	6 cut(s)	180, 614, 986, 1238, 1541, 1577
MaeIII	GTNAC	4 cut(s)	466, 538, 896, 1552
MalI	GATC	6 cut(s)	520, 558, 633, 936, 1154, 1461
MboI	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
MboII	GAAGA	9 cut(s)	317, 327, 517, 634, 1039, 1115, 1155, 1286, 1576
MflI	RGATCY	3 cut(s)	518, 556, 1152
MhlI	GDGCHC	2 cut(s)	1070, 1098
MlsI	TGGCCA	1 cut(s)	812
MluCI	AATT	8 cut(s)	290, 638, 758, 926, 944, 981, 1182, 1269
MluNI	TGGCCA	1 cut(s)	812
MmeI	TCCRAC	1 cut(s)	198
Mox20I	TGGCCA	1 cut(s)	812
Mph1103I	ATGCAT	2 cut(s)	69, 1389
MroI	TCCGGA	3 cut(s)	418, 937, 1372
MroXI	GAANNNNTTC	1 cut(s)	867
MscI	TGGCCA	1 cut(s)	812
MseI	TTAA	3 cut(s)	798, 830, 992
MslI	CAYNNNNRTG	1 cut(s)	1392
Msp20I	TGGCCA	1 cut(s)	812
MspA1I	CMGCKG	2 cut(s)	221, 1073
MspI	CCGG	5 cut(s)	315, 419, 544, 938, 1373
MspR9I	CCNGG	4 cut(s)	43, 371, 544, 1149
MvaI	CCWGG	3 cut(s)	43, 371, 1149
MvnI	CGCG	1 cut(s)	221
MwoI	GCNNNNNNNGC	5 cut(s)	116, 221, 233, 809, 818
NciI	CCSGG	1 cut(s)	544
NcoI	CCATGG	4 cut(s)	204, 807, 957, 1014
NdeI	CATATG	1 cut(s)	1022
NdeII	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
NlaIII	CATG	8 cut(s)	71, 208, 568, 811, 961, 1018, 1387, 1447
NlaIV	GGNNCC	3 cut(s)	520, 1145, 1154
NmuCI	GTSAC	4 cut(s)	466, 538, 896, 1552
NsiI	ATGCAT	2 cut(s)	69, 1389
NspV	TTCGAA	1 cut(s)	624
PagI	TCATGA	1 cut(s)	564
PasI	CCCWGGG	1 cut(s)	1148
PceI	AGGCCT	1 cut(s)	186
PdmI	GAANNNNTTC	1 cut(s)	867
PfeI	GAWTC	4 cut(s)	31, 844, 1233, 1306
PflMI	CCANNNNNTGG	1 cut(s)	734
PkrI	GCNGC	9 cut(s)	223, 226, 249, 252, 376, 805, 1318, 1498, 1501
Psp124BI	GAGCTC	1 cut(s)	1098
Psp6I	CCWGG	3 cut(s)	41, 369, 1147
PspEI	GGTNACC	2 cut(s)	538, 896
PspFI	CCCAGC	1 cut(s)	1062
PspGI	CCWGG	3 cut(s)	41, 369, 1147
PspN4I	GGNNCC	3 cut(s)	520, 1145, 1154
PspPI	GGNCC	2 cut(s)	940, 1380
PstI	CTGCAG	1 cut(s)	1501
PsuI	RGATCY	3 cut(s)	518, 556, 1152
PvuII	CAGCTG	1 cut(s)	1073
RsaI	GTAC	1 cut(s)	1281
RsaNI	GTAC	1 cut(s)	1280
RseI	CAYNNNNRTG	1 cut(s)	1392
SacI	GAGCTC	1 cut(s)	1098
SacII	CCGCGG	1 cut(s)	222
SaqAI	TTAA	3 cut(s)	798, 830, 992
SatI	GCNGC	9 cut(s)	222, 225, 248, 251, 375, 804, 1317, 1497, 1500
Sau3AI	GATC	6 cut(s)	518, 556, 631, 934, 1152, 1459
Sau96I	GGNCC	2 cut(s)	940, 1380
ScrFI	CCNGG	4 cut(s)	43, 371, 544, 1149
SduI	GDGCHC	2 cut(s)	1070, 1098
SexAI	ACCWGGT	1 cut(s)	41
SfaNI	GCATC	4 cut(s)	54, 583, 649, 1531
SfcI	CTRYAG	2 cut(s)	907, 1497
Sfr303I	CCGCGG	1 cut(s)	222
SfuI	TTCGAA	1 cut(s)	624
SgrBI	CCGCGG	1 cut(s)	222
SinI	GGWCC	2 cut(s)	940, 1380
SmiMI	CAYNNNNRTG	1 cut(s)	1392
SmlI	CTYRAG	5 cut(s)	97, 104, 188, 822, 1133
SmoI	CTYRAG	5 cut(s)	97, 104, 188, 822, 1133
Sse9I	AATT	8 cut(s)	290, 638, 758, 926, 944, 981, 1182, 1269
SseBI	AGGCCT	1 cut(s)	186
SsiI	CCGC	5 cut(s)	75, 219, 221, 318, 375
SspMI	CTAG	6 cut(s)	180, 614, 986, 1238, 1541, 1577
SstI	GAGCTC	1 cut(s)	1098
StuI	AGGCCT	1 cut(s)	186
StyD4I	CCNGG	4 cut(s)	41, 369, 542, 1147
StyI	CCWWGG	4 cut(s)	204, 807, 957, 1014
TaaI	ACNGT	3 cut(s)	922, 1258, 1334
TaqI	TCGA	4 cut(s)	624, 924, 1218, 1267
TasI	AATT	8 cut(s)	290, 638, 758, 926, 944, 981, 1182, 1269
TatI	WGTACW	1 cut(s)	1279
TauI	GCSGC	2 cut(s)	224, 377
TfiI	GAWTC	4 cut(s)	31, 844, 1233, 1306
Tru1I	TTAA	3 cut(s)	798, 830, 992
Tru9I	TTAA	3 cut(s)	798, 830, 992
TscAI	CASTG	1 cut(s)	1261
TseFI	GTSAC	4 cut(s)	466, 538, 896, 1552
TseI	GCWGC	7 cut(s)	224, 247, 250, 803, 1316, 1496, 1499
Tsp45I	GTSAC	4 cut(s)	466, 538, 896, 1552
TspDTI	ATGAA	7 cut(s)	165, 287, 581, 1052, 1155, 1432, 1521
TspGWI	ACGGA	2 cut(s)	565, 1299
TspRI	CASTG	1 cut(s)	1261
Van91I	CCANNNNNTGG	1 cut(s)	734
VpaK11BI	GGWCC	2 cut(s)	940, 1380
XapI	RAATTY	5 cut(s)	638, 758, 926, 981, 1182
XbaI	TCTAGA	3 cut(s)	613, 985, 1576
XcmI	CCANNNNNNNNNTGG	1 cut(s)	367
XmnI	GAANNNNTTC	1 cut(s)	867
XspI	CTAG	6 cut(s)	180, 614, 986, 1238, 1541, 1577
Zsp2I	ATGCAT	2 cut(s)	69, 1389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.