FvH4_6g53030

Beta-glucosidase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009710
Physical Location & Seq
Forward (+)
38943714 .. 38953008
9295 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53030.t7

Sequence Viewer

Length: 1788 bp
ATGGCTCTCTCTCTAAGCTTTTCGCTGACAACGTCGGCAACAACAGCGCCTTTGAGCTCCTTCAAGAGACTACCGAGCCGTATTCCTACGGTGAAAGCCCTAGGAGTGTTCCCATCTAAGGGAATAGATCATAAGAGGGCCATCAGAATTCGACCATGCTCTTCATTGGGAGATCTTACTTCAGCACTCACAAGTGCAGTTACTACTACTGTGGCCGTCGCTGTTGACACTGCGGCCGCGGTTGTTGATACCACAACCACAACCACAACCACGACCGAAACTGCAGAAGTAACCAGGGCTGATTTTCCTGCTGATTTCAAGTTTGGTTGCGCCACTTCTGCTTTCCAGACAGAAGGATCTGGAACCGAAGGAGGGAGAGGACCGGCCACATGGGATGATTACATACAAGATACCACAGTAGATGGCACAGAAATAGCAGTTGATTCTTACAACCGCTACAAGGACGATGTGCAACTGCTGAAGAATATGGGAGTGGATACATACAGATTCTCCATGTCATGGTCAAGACTATTGCCTGACGGAACCATAGACGGTGGAATAAACCAGGAGGGTATCGACTTCTACAACAACTTCATCGACGAACTGATCGCAAATGGGATAACTCCATTTGTGACACTGTTCCACTTCGACTTGCCCACAGCACTGAAGGACAAGTACAACGGTTTCTTGAGCATTGACATTGTGGAGGATTTCAAAGCATATGCTGATTTGTGTTTCAAAACTTTTGGCGATCGAGTGAAGTACTGGGCTACTATCAATGAGCCTCAAGTATGGGGGCAATACGGCTTCACTAACACTGCTGCGGATGCAAACGCTGCCACTGATCCTTATGTCTCTTCGCATAATGTCATATTAGCCCATGCTACAGCTGCCAAGCTCTACAAGAACACATACCAGTCAACGCAAGGAGGAGAGATCGGAATCCCGATCGTGGTTGAATGGTTTGAGCCATTCGAGGACACCCCGCAAGATGTCTTTGCAGCCAAAAGAGCATTCGACTTTTTGACAGGATGGTTTCTGGAGCCCCTAGTGTATGGTGATTACCCATTTGTTATGAAGGCTTTGGTGAGGGATGGCCTTCCAGAGTTCACAGATGAGCAGAAAGAGTTGGTAAAGGGCTCATATGATTACATTGGGGTGAACTACTACACTTCCAGATTCGCCACTTCTGTACCAATAACTAGCAACGATGTCTACACAACTATGGATCAGTACCAGCATGCTACCATCACAGTGGAAGGATCAGACGGGGAGCCCATAGGCGATGCAACACCAGGGAGCAGTTCGATCTACGTCTATCCAGAAGGATTAAGAAAGGGATTGACATTACTGAAGGAATACAACAATCCCAAAATCTACGTTACTGAGAATGGATATCCGGACGCAAGAGATGACACCCTTTCAGTGGAAGAAGCTTCGGTTGATGATGCTCGAATTCAGCACATCAAGGATCATCTCGCCGCTGTCAAAGCCGCCAGAGATGCGGGAGCAGATGTGCAGGGTTACCTGATGTGGGCACTGATGGACTGCGTGGAGATGGGATCTGGCTACGCGGATCATCTCGCCGCTGTCAAAGCCGCCAGAGATGCGGGAGCAGATGTGCAGGGTTACCTGATGTGGGCACTGATGGACTGCGTGGAGATGGGATCTGGCTACGCAGTTCGGTTTGGCCTCAACTACACTGATTACCTCAATGACTTGGTCCGAACTCCAAAGAAATCCGCCGCCTGGCTCAAAGATTTCTTGGCATCAACCACTTCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

596

Amino Acids

65.18

Weight (kDa)

4.58

Isoelectric Point (pI)

17.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 98 - 553 1.7e-135 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 519
AccI GTMKAC 1 cut(s) 1215
AccII CGCG 2 cut(s) 239, 1574
AccIII TCCGGA 1 cut(s) 1399
AclWI GGATC 8 cut(s) 364, 839, 1236, 1270, 1479, 1570, 1584, 1675
AcoI YGGCCR 3 cut(s) 213, 234, 384
AcsI RAATTY 2 cut(s) 147, 1455
AcuI CTGAAG 4 cut(s) 165, 500, 686, 1373
AfaI GTAC 4 cut(s) 677, 764, 1194, 1235
AgsI TTSAA 5 cut(s) 64, 319, 715, 739, 959
AjnI CCWGG 4 cut(s) 293, 564, 1294, 1748
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AluBI AGCT 5 cut(s) 18, 57, 890, 898, 1436
AluI AGCT 5 cut(s) 18, 57, 890, 898, 1436
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 2 cut(s) 61, 859
AlwI GGATC 8 cut(s) 364, 839, 1236, 1270, 1479, 1570, 1584, 1675
Aor13HI TCCGGA 1 cut(s) 1399
AoxI GGCC 6 cut(s) 138, 213, 234, 384, 1096, 1690
ApeKI GCWGC 4 cut(s) 821, 836, 890, 1001
ApoI RAATTY 2 cut(s) 147, 1455
AspA2I CCTAGG 1 cut(s) 100
AspLEI GCGC 2 cut(s) 49, 332
AspS9I GGNCC 3 cut(s) 138, 380, 1723
AsuHPI GGTGA 4 cut(s) 103, 1070, 1099, 1171
AvaII GGWCC 2 cut(s) 380, 1723
AvrII CCTAGG 1 cut(s) 100
BaeGI GKGCMC 2 cut(s) 1540, 1645
BaeI ACNNNNGTAYC 4 cut(s) 402, 435, 1176, 1209
BanII GRGCYC 4 cut(s) 59, 1047, 1142, 1278
BarI GAAGNNNNNNTAC 2 cut(s) 659, 691
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 4 cut(s) 808, 823, 877, 1013
BceAI ACGGC 3 cut(s) 63, 200, 820
BciT130I CCWGG 4 cut(s) 295, 566, 1296, 1750
BciVI GTATCC 1 cut(s) 490
BcoDI GTCTC 2 cut(s) 61, 859
BfaI CTAG 3 cut(s) 101, 1049, 1203
BfmI CTRYAG 2 cut(s) 282, 885
BfoI RGCGCY 1 cut(s) 50
BfuI GTATCC 1 cut(s) 490
BglII AGATCT 1 cut(s) 172
BlnI CCTAGG 1 cut(s) 100
BmcAI AGTACT 1 cut(s) 764
Bme1390I CCNGG 4 cut(s) 295, 566, 1296, 1750
Bme18I GGWCC 2 cut(s) 380, 1723
BmgT120I GGNCC 3 cut(s) 138, 380, 1723
BmiI GGNNCC 4 cut(s) 364, 544, 1044, 1275
BmrFI CCNGG 4 cut(s) 295, 566, 1296, 1750
BmrI ACTGGG 1 cut(s) 775
BmsI GCATC 6 cut(s) 817, 1276, 1438, 1492, 1597, 1778
BmuI ACTGGG 1 cut(s) 775
BpmI CTGGAG 1 cut(s) 1061
BpuEI CTTGAG 2 cut(s) 709, 771
BsaBI GATNNNNATC 1 cut(s) 941
BsaJI CCNNGG 4 cut(s) 100, 237, 294, 1295
BsaWI WCCGGW 1 cut(s) 1399
BsaXI ACNNNNNCTCC 6 cut(s) 494, 524, 1500, 1530, 1605, 1635
Bse118I RCCGGY 1 cut(s) 382
Bse1I ACTGG 2 cut(s) 770, 916
Bse8I GATNNNNATC 1 cut(s) 941
BseAI TCCGGA 1 cut(s) 1399
BseBI CCWGG 4 cut(s) 295, 566, 1296, 1750
BseDI CCNNGG 4 cut(s) 100, 237, 294, 1295
BseGI GGATG 4 cut(s) 400, 832, 1037, 1099
BseJI GATNNNNATC 1 cut(s) 941
BseMII CTCAG 1 cut(s) 1377
BseNI ACTGG 2 cut(s) 770, 916
BseRI GAGGAG 1 cut(s) 945
BseSI GKGCMC 2 cut(s) 1540, 1645
BseX3I CGGCCG 1 cut(s) 234
BseXI GCAGC 4 cut(s) 808, 823, 877, 1013
BsgI GTGCAG 3 cut(s) 216, 1538, 1643
Bsh1236I CGCG 2 cut(s) 239, 1574
Bsh1285I CGRYCG 4 cut(s) 237, 276, 754, 951
BshFI GGCC 6 cut(s) 140, 215, 236, 386, 1098, 1692
BsiEI CGRYCG 4 cut(s) 237, 276, 754, 951
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 2 cut(s) 383, 1400
BsmAI GTCTC 2 cut(s) 61, 859
BsmI GAATGC 1 cut(s) 1013
BsnI GGCC 6 cut(s) 140, 215, 236, 386, 1098, 1692
Bsp1286I GDGCHC 6 cut(s) 59, 1047, 1142, 1278, 1540, 1645
Bsp13I TCCGGA 1 cut(s) 1399
BspANI GGCC 6 cut(s) 140, 215, 236, 386, 1098, 1692
BspCNI CTCAG 1 cut(s) 1378
BspEI TCCGGA 1 cut(s) 1399
BspFNI CGCG 2 cut(s) 239, 1574
BspLI GGNNCC 4 cut(s) 364, 544, 1044, 1275
BspMAI CTGCAG 1 cut(s) 286
BspPI GGATC 8 cut(s) 364, 839, 1236, 1270, 1479, 1570, 1584, 1675
BspQI GCTCTTC 1 cut(s) 166
BsrFI RCCGGY 1 cut(s) 382
BsrI ACTGG 2 cut(s) 770, 916
BssAI RCCGGY 1 cut(s) 382
BssECI CCNNGG 4 cut(s) 100, 237, 294, 1295
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 4 cut(s) 295, 566, 1296, 1750
Bst4CI ACNGT 7 cut(s) 91, 211, 418, 554, 639, 683, 1255
Bst6I CTCTTC 2 cut(s) 166, 862
BstAPI GCANNNNNTGC 1 cut(s) 836
BstC8I GCNNGC 1 cut(s) 1242
BstDEI CTNAG 3 cut(s) 14, 117, 1386
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 2 cut(s) 1523, 1628
BstF5I GGATG 4 cut(s) 400, 832, 1037, 1099
BstFNI CGCG 2 cut(s) 239, 1574
BstH2I RGCGCY 1 cut(s) 50
BstHHI GCGC 2 cut(s) 49, 332
BstMAI GTCTC 2 cut(s) 61, 859
BstMCI CGRYCG 4 cut(s) 237, 276, 754, 951
BstNI CCWGG 4 cut(s) 295, 566, 1296, 1750
BstNSI RCATGY 1 cut(s) 1244
BstPI GGTNACC 2 cut(s) 1523, 1628
BstSCI CCNGG 4 cut(s) 293, 564, 1294, 1748
BstSFI CTRYAG 2 cut(s) 282, 885
BstSLI GKGCMC 2 cut(s) 1540, 1645
BstUI CGCG 2 cut(s) 239, 1574
BstV1I GCAGC 4 cut(s) 808, 823, 877, 1013
BstX2I RGATCY 4 cut(s) 172, 356, 1562, 1667
BstXI CCANNNNNNTGG 1 cut(s) 1255
BstYI RGATCY 4 cut(s) 172, 356, 1562, 1667
BstZI CGGCCG 1 cut(s) 234
BsuI GTATCC 1 cut(s) 490
BsuRI GGCC 6 cut(s) 140, 215, 236, 386, 1098, 1692
BtgI CCRYGG 1 cut(s) 237
BtgZI GCGATG 1 cut(s) 1299
BtsCI GGATG 4 cut(s) 400, 832, 1037, 1099
BtsI GCAGTG 2 cut(s) 228, 816
Cac8I GCNNGC 1 cut(s) 1242
CciNI GCGGCCGC 1 cut(s) 234
CfoI GCGC 2 cut(s) 49, 332
Cfr10I RCCGGY 1 cut(s) 382
Cfr13I GGNCC 3 cut(s) 138, 380, 1723
Cfr42I CCGCGG 1 cut(s) 240
CseI GACGC 1 cut(s) 1412
Csp6I GTAC 4 cut(s) 676, 763, 1193, 1234
CviAII CATG 6 cut(s) 156, 390, 514, 519, 881, 1241
CviQI GTAC 4 cut(s) 676, 763, 1193, 1234
DdeI CTNAG 3 cut(s) 14, 117, 1386
EaeI YGGCCR 3 cut(s) 213, 234, 384
EagI CGGCCG 1 cut(s) 234
Eam1104I CTCTTC 2 cut(s) 166, 862
EarI CTCTTC 2 cut(s) 166, 862
EciI GGCGGA 1 cut(s) 1732
Ecl136II GAGCTC 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 234
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 4 cut(s) 59, 1047, 1142, 1278
Eco32I GATATC 1 cut(s) 1397
Eco47I GGWCC 2 cut(s) 380, 1723
Eco52I CGGCCG 1 cut(s) 234
Eco53kI GAGCTC 1 cut(s) 57
Eco57I CTGAAG 4 cut(s) 165, 500, 686, 1373
Eco91I GGTNACC 2 cut(s) 1523, 1628
EcoICRI GAGCTC 1 cut(s) 57
EcoO65I GGTNACC 2 cut(s) 1523, 1628
EcoRI GAATTC 2 cut(s) 147, 1455
EcoRII CCWGG 4 cut(s) 293, 564, 1294, 1748
EcoRV GATATC 1 cut(s) 1397
EcoT14I CCWWGG 1 cut(s) 100
EcoT38I GRGCYC 4 cut(s) 59, 1047, 1142, 1278
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 6 cut(s) 159, 393, 517, 522, 884, 1244
FatI CATG 6 cut(s) 155, 389, 513, 518, 880, 1240
FauI CCCGC 3 cut(s) 993, 1498, 1603
FauNDI CATATG 2 cut(s) 721, 1144
FblI GTMKAC 1 cut(s) 1215
FokI GGATG 4 cut(s) 407, 839, 1044, 1106
FriOI GRGCYC 4 cut(s) 59, 1047, 1142, 1278
FspBI CTAG 3 cut(s) 101, 1049, 1203
GlaI GCGC 2 cut(s) 48, 331
GsuI CTGGAG 1 cut(s) 1061
HaeII RGCGCY 1 cut(s) 50
HaeIII GGCC 6 cut(s) 140, 215, 236, 386, 1098, 1692
HapII CCGG 2 cut(s) 383, 1400
HgaI GACGC 1 cut(s) 1412
HhaI GCGC 2 cut(s) 49, 332
Hin1II CATG 6 cut(s) 159, 393, 517, 522, 884, 1244
Hin6I GCGC 2 cut(s) 47, 330
HinP1I GCGC 2 cut(s) 47, 330
HincII GTYRAC 2 cut(s) 226, 921
HindII GTYRAC 2 cut(s) 226, 921
HindIII AAGCTT 2 cut(s) 16, 1434
HinfI GANTC 4 cut(s) 443, 507, 942, 1179
HpaII CCGG 2 cut(s) 383, 1400
HphI GGTGA 4 cut(s) 103, 1070, 1099, 1171
Hpy166II GTNNAC 5 cut(s) 226, 921, 1110, 1162, 1216
Hpy188I TCNGA 4 cut(s) 146, 941, 1267, 1727
Hpy8I GTNNAC 5 cut(s) 226, 921, 1110, 1162, 1216
Hpy99I CGWCG 3 cut(s) 37, 221, 602
HpyAV CCTTC 9 cut(s) 70, 347, 362, 661, 1072, 1109, 1253, 1319, 1348
HpyCH4III ACNGT 7 cut(s) 91, 211, 418, 554, 639, 683, 1255
HpyCH4IV ACGT 3 cut(s) 32, 1314, 1380
HpyCH4V TGCA 8 cut(s) 197, 284, 472, 830, 1001, 1289, 1519, 1624
HpyF3I CTNAG 3 cut(s) 14, 117, 1386
HpySE526I ACGT 3 cut(s) 32, 1314, 1380
Hsp92II CATG 6 cut(s) 159, 393, 517, 522, 884, 1244
HspAI GCGC 2 cut(s) 47, 330
Kpn2I TCCGGA 1 cut(s) 1399
KspI CCGCGG 1 cut(s) 240
LguI GCTCTTC 1 cut(s) 166
LmnI GCTCC 6 cut(s) 62, 1042, 1273, 1299, 1508, 1613
Lsp1109I GCAGC 4 cut(s) 808, 823, 877, 1013
LweI GCATC 6 cut(s) 817, 1276, 1438, 1492, 1597, 1778
MaeI CTAG 3 cut(s) 101, 1049, 1203
MaeII ACGT 3 cut(s) 32, 1314, 1380
MaeIII GTNAC 6 cut(s) 199, 289, 631, 1381, 1523, 1628
MboII GAAGA 4 cut(s) 153, 493, 849, 1442
MflI RGATCY 4 cut(s) 172, 356, 1562, 1667
MhlI GDGCHC 6 cut(s) 59, 1047, 1142, 1278, 1540, 1645
MluCI AATT 2 cut(s) 147, 1455
MroI TCCGGA 1 cut(s) 1399
MseI TTAA 1 cut(s) 1331
MslI CAYNNNNRTG 3 cut(s) 1157, 1223, 1253
MspA1I CMGCKG 4 cut(s) 239, 890, 1484, 1589
MspI CCGG 2 cut(s) 383, 1400
MspR9I CCNGG 4 cut(s) 295, 566, 1296, 1750
Mva1269I GAATGC 1 cut(s) 1013
MvaI CCWGG 4 cut(s) 295, 566, 1296, 1750
MvnI CGCG 2 cut(s) 239, 1574
NdeI CATATG 2 cut(s) 721, 1144
NlaIII CATG 6 cut(s) 159, 393, 517, 522, 884, 1244
NlaIV GGNNCC 4 cut(s) 364, 544, 1044, 1275
NmuCI GTSAC 1 cut(s) 631
NotI GCGGCCGC 1 cut(s) 234
NspI RCATGY 1 cut(s) 1244
PaeI GCATGC 1 cut(s) 1244
PciSI GCTCTTC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 1013
PfeI GAWTC 4 cut(s) 443, 507, 942, 1179
PflFI GACNNNGTC 2 cut(s) 31, 1721
PflMI CCANNNNNTGG 1 cut(s) 519
Ple19I CGATCG 2 cut(s) 754, 951
Psp124BI GAGCTC 1 cut(s) 59
Psp6I CCWGG 4 cut(s) 293, 564, 1294, 1748
PspEI GGTNACC 2 cut(s) 1523, 1628
PspGI CCWGG 4 cut(s) 293, 564, 1294, 1748
PspN4I GGNNCC 4 cut(s) 364, 544, 1044, 1275
PspPI GGNCC 3 cut(s) 138, 380, 1723
PstI CTGCAG 1 cut(s) 286
PsuI RGATCY 4 cut(s) 172, 356, 1562, 1667
PsyI GACNNNGTC 2 cut(s) 31, 1721
PvuI CGATCG 2 cut(s) 754, 951
PvuII CAGCTG 1 cut(s) 890
RsaI GTAC 4 cut(s) 677, 764, 1194, 1235
RsaNI GTAC 4 cut(s) 676, 763, 1193, 1234
RseI CAYNNNNRTG 3 cut(s) 1157, 1223, 1253
SacI GAGCTC 1 cut(s) 59
SacII CCGCGG 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 166
SaqAI TTAA 1 cut(s) 1331
Sau96I GGNCC 3 cut(s) 138, 380, 1723
ScaI AGTACT 1 cut(s) 764
ScrFI CCNGG 4 cut(s) 295, 566, 1296, 1750
SduI GDGCHC 6 cut(s) 59, 1047, 1142, 1278, 1540, 1645
SfaNI GCATC 6 cut(s) 817, 1276, 1438, 1492, 1597, 1778
SfcI CTRYAG 2 cut(s) 282, 885
Sfr303I CCGCGG 1 cut(s) 240
SgrBI CCGCGG 1 cut(s) 240
SinI GGWCC 2 cut(s) 380, 1723
SmiMI CAYNNNNRTG 3 cut(s) 1157, 1223, 1253
SmlI CTYRAG 2 cut(s) 688, 786
SmoI CTYRAG 2 cut(s) 688, 786
SphI GCATGC 1 cut(s) 1244
Sse9I AATT 2 cut(s) 147, 1455
SspMI CTAG 3 cut(s) 101, 1049, 1203
SstI GAGCTC 1 cut(s) 59
StyD4I CCNGG 4 cut(s) 293, 564, 1294, 1748
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 7 cut(s) 91, 211, 418, 554, 639, 683, 1255
TaiI ACGT 3 cut(s) 35, 1317, 1383
TaqI TCGA 9 cut(s) 151, 576, 597, 648, 754, 975, 1017, 1307, 1453
TaqII GACCGA 1 cut(s) 290
TasI AATT 2 cut(s) 147, 1455
TatI WGTACW 2 cut(s) 675, 762
TauI GCSGC 7 cut(s) 236, 239, 1484, 1496, 1589, 1601, 1748
TfiI GAWTC 4 cut(s) 443, 507, 942, 1179
Tru1I TTAA 1 cut(s) 1331
Tru9I TTAA 1 cut(s) 1331
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 4 cut(s) 821, 836, 890, 1001
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 3 cut(s) 153, 583, 1091
TspGWI ACGGA 1 cut(s) 555
Tth111I GACNNNGTC 2 cut(s) 31, 1721
Van91I CCANNNNNTGG 1 cut(s) 519
VpaK11BI GGWCC 2 cut(s) 380, 1723
XapI RAATTY 2 cut(s) 147, 1455
XceI RCATGY 1 cut(s) 1244
XmaJI CCTAGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 1215
XspI CTAG 3 cut(s) 101, 1049, 1203
ZrmI AGTACT 1 cut(s) 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.