MD01G1026300.v1.1

Protein of unknown function (DUF632)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011192
Physical Location & Seq
Forward (+)
9873624 .. 9875588
1965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1026300.v1.1.491

Sequence Viewer

Length: 951 bp
ATGATTGCCGGAAGTCATTCCTCCACTCTGGAGAGACTATATGCCTGGGAAAGAAAACTCTATGACGAAGTAAAGGCAAGTGAATCTATCAGGAAGGTGTATGACCGGAAATGTGATCAACTTAGGAATCAATTTGCAGAGGATTGTAGCAGTCAAGTTATTGATAAAACCCGGGCGATTGTGAAGGATCTACATTCACGAATAAGAGTGGCAATTCATGCTGTTGATTCAATATCAAAGCGGATTGAGAAAATGAGAGACGAAGAATTGCATCCACAACTTTTAGAACTTACTCAGGGATTGACAAGAATGTGGAAGGCCATGCTTGAATGCCATCATGCGCAATATATAACCATCTCACTCGCATATCATTCAAAGAGCTCAACAGTGACTTCCCAAGGAGATTCCCGCAGGCAGATCATGGCTCAACTTTTGGATGAGATTGAGTGTTTCGGCTTAAGCTTTGCAAATTGGATCAACAGCCATTCAACATATGTGGAAGCTCTCAATGGTTGGCTGCAAAACTGCATCATGCAACCACGGGAGCGTACCAAAAGCAGAAGGCCATTTTCCCCTCGTCGAGTTGTGGCCCCACCTATATTTGTTCTCTTTCGAGATTGGGGAGCTGGGATCAGAGCATTGCCTTCCAATGAACTTACAGATGCAATCAGGACCTTCTTATCAGATCTGCGTCATTTGATGGAACAAGAAGCAGTTGCACAGAAAAACCAGGGGACAGCTGATGCAAACAACGGAGAAGCAGAGAACAAAGCGGAAGAGAACGGCAAAGAATCATCTCCAAGCCTGAGCTGCATACATACAAGCTTGAGAAAGGTTCTCGATAGACTAACTAAATTTTCGGAGGCATCATTAAAGATGTATGAAGATATCAGACAGAAAAGTGAAGCAGCTCGAATTGCATATCTCAACTGCAGGCCTGTTAGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF630 DUF632

Protein Analysis

317

Amino Acids

36.35

Weight (kDa)

8.89

Isoelectric Point (pI)

52.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF632 PF04782 1 - 231 5.2e-94 Protein of unknown function (DUF632)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AciI CCGC 3 cut(s) 241, 409, 773
AclWI GGATC 3 cut(s) 195, 482, 638
AcsI RAATTY 1 cut(s) 854
AfaI GTAC 1 cut(s) 550
AflII CTTAAG 1 cut(s) 457
AgsI TTSAA 4 cut(s) 231, 329, 375, 489
AjnI CCWGG 2 cut(s) 44, 729
AluBI AGCT 8 cut(s) 381, 462, 503, 626, 740, 810, 825, 911
AluI AGCT 8 cut(s) 381, 462, 503, 626, 740, 810, 825, 911
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 2 cut(s) 28, 252
AlwI GGATC 3 cut(s) 195, 482, 638
Ama87I CYCGRG 1 cut(s) 171
AoxI GGCC 4 cut(s) 318, 563, 588, 935
ApeKI GCWGC 3 cut(s) 517, 810, 908
ApoI RAATTY 1 cut(s) 854
Asp700I GAANNNNTTC 1 cut(s) 16
AspLEI GCGC 1 cut(s) 343
AspS9I GGNCC 2 cut(s) 589, 672
AsuC2I CCSGG 2 cut(s) 172, 173
AvaI CYCGRG 1 cut(s) 171
AvaII GGWCC 1 cut(s) 672
BanII GRGCYC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 3 cut(s) 504, 797, 920
BccI CCATC 3 cut(s) 342, 362, 694
BceAI ACGGC 1 cut(s) 799
BciT130I CCWGG 2 cut(s) 46, 731
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 2 cut(s) 172, 173
BcoDI GTCTC 2 cut(s) 28, 252
BfmI CTRYAG 1 cut(s) 931
BfrI CTTAAG 1 cut(s) 457
BglII AGATCT 1 cut(s) 685
BisI GCNGC 3 cut(s) 518, 811, 909
BlsI GCNGC 3 cut(s) 519, 812, 910
Bme1390I CCNGG 4 cut(s) 46, 172, 173, 731
Bme18I GGWCC 1 cut(s) 672
BmeT110I CYCGRG 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 589, 672
BmiI GGNNCC 1 cut(s) 591
BmrFI CCNGG 4 cut(s) 46, 172, 173, 731
BmsI GCATC 5 cut(s) 280, 537, 652, 733, 875
BpmI CTGGAG 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 806
BpuEI CTTGAG 1 cut(s) 847
BpuMI CCSGG 2 cut(s) 172, 173
BsaJI CCNNGG 5 cut(s) 45, 171, 397, 539, 730
BsaWI WCCGGW 1 cut(s) 105
Bse3DI GCAATG 1 cut(s) 638
BseBI CCWGG 2 cut(s) 46, 731
BseDI CCNNGG 5 cut(s) 45, 171, 397, 539, 730
BseGI GGATG 2 cut(s) 271, 442
BseMI GCAATG 1 cut(s) 638
BseMII CTCAG 2 cut(s) 308, 797
BseXI GCAGC 3 cut(s) 504, 797, 920
BseYI CCCAGC 1 cut(s) 626
BshFI GGCC 4 cut(s) 320, 565, 590, 937
BsiHKAI GWGCWC 1 cut(s) 383
BsiHKCI CYCGRG 1 cut(s) 171
BsiSI CCGG 3 cut(s) 9, 106, 172
BslFI GGGAC 1 cut(s) 748
BsmAI GTCTC 2 cut(s) 28, 252
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 748
BsmI GAATGC 1 cut(s) 335
BsnI GGCC 4 cut(s) 320, 565, 590, 937
BsoBI CYCGRG 1 cut(s) 171
Bsp1286I GDGCHC 1 cut(s) 383
Bsp143I GATC 6 cut(s) 115, 187, 417, 474, 630, 685
BspACI CCGC 3 cut(s) 241, 409, 773
BspANI GGCC 4 cut(s) 320, 565, 590, 937
BspCNI CTCAG 2 cut(s) 307, 798
BspLI GGNNCC 1 cut(s) 591
BspMAI CTGCAG 1 cut(s) 935
BspPI GGATC 3 cut(s) 195, 482, 638
BspTI CTTAAG 1 cut(s) 457
BsrDI GCAATG 1 cut(s) 638
BssECI CCNNGG 5 cut(s) 45, 171, 397, 539, 730
BssMI GATC 6 cut(s) 115, 187, 417, 474, 630, 685
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 2 cut(s) 46, 731
Bst4CI ACNGT 1 cut(s) 388
Bst6I CTCTTC 1 cut(s) 771
BstAFI CTTAAG 1 cut(s) 457
BstAPI GCANNNNNTGC 1 cut(s) 218
BstC8I GCNNGC 2 cut(s) 413, 935
BstDEI CTNAG 3 cut(s) 122, 294, 806
BstDSI CCRYGG 1 cut(s) 539
BstF5I GGATG 2 cut(s) 271, 442
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 6 cut(s) 118, 190, 420, 477, 633, 688
BstMAI GTCTC 2 cut(s) 28, 252
BstMBI GATC 6 cut(s) 115, 187, 417, 474, 630, 685
BstMWI GCNNNNNNNGC 3 cut(s) 218, 810, 917
BstNI CCWGG 2 cut(s) 46, 731
BstSCI CCNGG 4 cut(s) 44, 170, 171, 729
BstSFI CTRYAG 1 cut(s) 931
BstV1I GCAGC 3 cut(s) 504, 797, 920
BstX2I RGATCY 2 cut(s) 187, 685
BstYI RGATCY 2 cut(s) 187, 685
BsuRI GGCC 4 cut(s) 320, 565, 590, 937
BtgI CCRYGG 1 cut(s) 539
BtsCI GGATG 2 cut(s) 271, 442
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 413, 935
CfoI GCGC 1 cut(s) 343
Cfr13I GGNCC 2 cut(s) 589, 672
Cfr9I CCCGGG 1 cut(s) 171
CseI GACGC 1 cut(s) 680
Csp6I GTAC 1 cut(s) 549
CspCI CAANNNNNGTGG 2 cut(s) 477, 512
CviAII CATG 5 cut(s) 218, 322, 338, 421, 532
CviQI GTAC 1 cut(s) 549
DdeI CTNAG 3 cut(s) 122, 294, 806
DpnI GATC 6 cut(s) 117, 189, 419, 476, 632, 687
DpnII GATC 6 cut(s) 115, 187, 417, 474, 630, 685
Eam1104I CTCTTC 1 cut(s) 771
EarI CTCTTC 1 cut(s) 771
Ecl136II GAGCTC 1 cut(s) 381
Eco130I CCWWGG 1 cut(s) 397
Eco147I AGGCCT 1 cut(s) 937
Eco24I GRGCYC 1 cut(s) 383
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 1 cut(s) 672
Eco53kI GAGCTC 1 cut(s) 381
Eco88I CYCGRG 1 cut(s) 171
EcoICRI GAGCTC 1 cut(s) 381
EcoO109I RGGNCCY 1 cut(s) 672
EcoRII CCWGG 2 cut(s) 44, 729
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 397
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 5 cut(s) 221, 325, 341, 424, 535
FaqI GGGAC 1 cut(s) 748
FatI CATG 5 cut(s) 217, 321, 337, 420, 531
FauI CCCGC 1 cut(s) 416
FauNDI CATATG 1 cut(s) 493
FbaI TGATCA 1 cut(s) 115
Fnu4HI GCNGC 3 cut(s) 518, 811, 909
FokI GGATG 2 cut(s) 258, 449
FriOI GRGCYC 1 cut(s) 383
Fsp4HI GCNGC 3 cut(s) 518, 811, 909
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 3 cut(s) 518, 811, 909
GsaI CCCAGC 1 cut(s) 630
GsuI CTGGAG 1 cut(s) 50
HaeIII GGCC 4 cut(s) 320, 565, 590, 937
HapII CCGG 3 cut(s) 9, 106, 172
HgaI GACGC 1 cut(s) 680
HhaI GCGC 1 cut(s) 343
Hin1II CATG 5 cut(s) 221, 325, 341, 424, 535
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HindIII AAGCTT 2 cut(s) 460, 823
HinfI GANTC 5 cut(s) 83, 127, 227, 404, 791
HpaII CCGG 3 cut(s) 9, 106, 172
Hpy188I TCNGA 4 cut(s) 635, 685, 862, 893
Hpy188III TCNNGA 6 cut(s) 29, 91, 198, 614, 670, 839
Hpy99I CGWCG 1 cut(s) 582
HpyAV CCTTC 6 cut(s) 88, 178, 310, 555, 654, 685
HpyCH4III ACNGT 1 cut(s) 388
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 810, 917
HpyF3I CTNAG 3 cut(s) 122, 294, 806
Hsp92II CATG 5 cut(s) 221, 325, 341, 424, 535
HspAI GCGC 1 cut(s) 341
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 6 cut(s) 115, 187, 417, 474, 630, 685
LmnI GCTCC 2 cut(s) 544, 623
Lsp1109I GCAGC 3 cut(s) 504, 797, 920
LweI GCATC 5 cut(s) 280, 537, 652, 733, 875
MaeIII GTNAC 1 cut(s) 388
MalI GATC 6 cut(s) 117, 189, 419, 476, 632, 687
MboI GATC 6 cut(s) 115, 187, 417, 474, 630, 685
MboII GAAGA 3 cut(s) 275, 788, 896
MflI RGATCY 2 cut(s) 187, 685
MhlI GDGCHC 1 cut(s) 383
MluCI AATT 6 cut(s) 131, 213, 266, 469, 854, 915
MnlI CCTC 4 cut(s) 31, 133, 585, 856
MroXI GAANNNNTTC 1 cut(s) 16
MseI TTAA 2 cut(s) 458, 872
MspA1I CMGCKG 1 cut(s) 740
MspCI CTTAAG 1 cut(s) 457
MspI CCGG 3 cut(s) 9, 106, 172
MspR9I CCNGG 4 cut(s) 46, 172, 173, 731
Mva1269I GAATGC 1 cut(s) 335
MvaI CCWGG 2 cut(s) 46, 731
MwoI GCNNNNNNNGC 3 cut(s) 218, 810, 917
NciI CCSGG 2 cut(s) 172, 173
NdeI CATATG 1 cut(s) 493
NdeII GATC 6 cut(s) 115, 187, 417, 474, 630, 685
NlaIII CATG 5 cut(s) 221, 325, 341, 424, 535
NlaIV GGNNCC 1 cut(s) 591
NmuCI GTSAC 1 cut(s) 388
NsbI TGCGCA 1 cut(s) 342
PceI AGGCCT 1 cut(s) 937
PctI GAATGC 1 cut(s) 335
PdmI GAANNNNTTC 1 cut(s) 16
PfeI GAWTC 5 cut(s) 83, 127, 227, 404, 791
PkrI GCNGC 3 cut(s) 519, 812, 910
PpuMI RGGWCCY 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 672
Psp6I CCWGG 2 cut(s) 44, 729
PspFI CCCAGC 1 cut(s) 626
PspGI CCWGG 2 cut(s) 44, 729
PspN4I GGNNCC 1 cut(s) 591
PspPI GGNCC 2 cut(s) 589, 672
PspPPI RGGWCCY 1 cut(s) 672
PstI CTGCAG 1 cut(s) 935
PsuI RGATCY 2 cut(s) 187, 685
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 1 cut(s) 550
RsaNI GTAC 1 cut(s) 549
SacI GAGCTC 1 cut(s) 383
SaqAI TTAA 2 cut(s) 458, 872
SatI GCNGC 3 cut(s) 518, 811, 909
Sau3AI GATC 6 cut(s) 115, 187, 417, 474, 630, 685
Sau96I GGNCC 2 cut(s) 589, 672
ScrFI CCNGG 4 cut(s) 46, 172, 173, 731
SduI GDGCHC 1 cut(s) 383
SfaNI GCATC 5 cut(s) 280, 537, 652, 733, 875
SfcI CTRYAG 1 cut(s) 931
SinI GGWCC 1 cut(s) 672
SmaI CCCGGG 1 cut(s) 173
SmlI CTYRAG 2 cut(s) 457, 826
SmoI CTYRAG 2 cut(s) 457, 826
Sse9I AATT 6 cut(s) 131, 213, 266, 469, 854, 915
SseBI AGGCCT 1 cut(s) 937
SsiI CCGC 3 cut(s) 241, 409, 773
SstI GAGCTC 1 cut(s) 383
StuI AGGCCT 1 cut(s) 937
StyD4I CCNGG 4 cut(s) 44, 170, 171, 729
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 1 cut(s) 388
TaqI TCGA 4 cut(s) 580, 613, 840, 913
TasI AATT 6 cut(s) 131, 213, 266, 469, 854, 915
TfiI GAWTC 5 cut(s) 83, 127, 227, 404, 791
Tru1I TTAA 2 cut(s) 458, 872
Tru9I TTAA 2 cut(s) 458, 872
TscAI CASTG 1 cut(s) 393
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 517, 810, 908
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 3 cut(s) 206, 666, 897
TspGWI ACGGA 1 cut(s) 768
TspMI CCCGGG 1 cut(s) 171
TspRI CASTG 1 cut(s) 393
Vha464I CTTAAG 1 cut(s) 457
VpaK11BI GGWCC 1 cut(s) 672
XapI RAATTY 1 cut(s) 854
XmaI CCCGGG 1 cut(s) 171
XmnI GAANNNNTTC 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.