MD01G1067000.v1.1

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0024381
Physical Location & Seq
Reverse (-)
17046923 .. 17048574
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1067000.v1.1.491

Sequence Viewer

Length: 546 bp
ATGGCCGTAGGGAGCACATTTGTGTACCTAGGCACAGTTGAGGACGATCTTAAGAGAGCATTACACACAGCCGTTGCACTTCTTACTGCACTCATGGTTTATACTAAGGCCAGAAGGCCGGGGAATATTATTCTAGTTATAGTAATTGGAACCATTGCTCTTCTTATTGGGTGGCTGATAGAATTGTCAACCAAGTTTAGGTCATTTTCATTTTCGACCAGATTCTCCTTAAATATGCTTGAGAGACTCCAAGCTGTTAACGGATGGCTCAAGAATCTCATTAAGACAACTTTTAGAAGATTTCGCTGGGGTTTTATACTTGCAGGTTTAGTAGCATTAGCCATTGCAGCAAGTAGCTGGGTACTGGAAACCAATGAAAGCTACTGGATTTGGCACAGCATTTGGCACATGACAATATATACATCTTCCTTCTTCTTCCTATGCTCAAAAGAAAGTCCTGCAAGTACGGTAGATACTGAGAATCAGATACCACCGACTGGAGCCTATGAATTGACTCGACAAGATTCATTTTCAAGAGGTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

182

Amino Acids

20.51

Weight (kDa)

9.41

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3522 PF12036 1 - 137 5.7e-20 Protein of unknown function (DUF3522)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024381)

Species Orthologous Gene IDs
malus_domestica MD01G1067000.v1.1
pyrus_communis pycom07g13140
rosa_multiflora Rmu_sc0004814.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 497
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 3 cut(s) 26, 363, 466
AfiI CCNNNNNNNGG 2 cut(s) 198, 497
AflII CTTAAG 1 cut(s) 50
AgsI TTSAA 1 cut(s) 534
AleI CACNNNNGTG 1 cut(s) 20
AluBI AGCT 3 cut(s) 254, 357, 381
AluI AGCT 3 cut(s) 254, 357, 381
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 238
AoxI GGCC 3 cut(s) 3, 108, 116
ApeKI GCWGC 1 cut(s) 347
AspA2I CCTAGG 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 120
AvrII CCTAGG 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 359
BccI CCATC 1 cut(s) 258
BceAI ACGGC 1 cut(s) 56
BcnI CCSGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 238
BfaI CTAG 2 cut(s) 29, 134
BfrI CTTAAG 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 314
BisI GCNGC 1 cut(s) 348
BlnI CCTAGG 1 cut(s) 28
BlsI GCNGC 1 cut(s) 349
Bme1390I CCNGG 1 cut(s) 120
BmiI GGNNCC 2 cut(s) 151, 502
BmrFI CCNGG 1 cut(s) 120
BpmI CTGGAG 1 cut(s) 519
BpuEI CTTGAG 2 cut(s) 254, 260
BpuMI CCSGG 1 cut(s) 120
BsaJI CCNNGG 2 cut(s) 28, 119
Bsc4I CCNNNNNNNGG 2 cut(s) 198, 497
Bse1I ACTGG 3 cut(s) 369, 389, 502
Bse3DI GCAATG 2 cut(s) 153, 342
BseDI CCNNGG 2 cut(s) 28, 119
BseGI GGATG 1 cut(s) 269
BseLI CCNNNNNNNGG 2 cut(s) 198, 497
BseMI GCAATG 2 cut(s) 153, 342
BseMII CTCAG 1 cut(s) 468
BseNI ACTGG 3 cut(s) 369, 389, 502
BseXI GCAGC 1 cut(s) 359
BseYI CCCAGC 2 cut(s) 306, 357
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 3 cut(s) 5, 110, 118
BsiHKAI GWGCWC 1 cut(s) 17
BsiSI CCGG 1 cut(s) 119
BslI CCNNNNNNNGG 2 cut(s) 198, 497
BsmAI GTCTC 1 cut(s) 238
BsnI GGCC 3 cut(s) 5, 110, 118
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 1 cut(s) 46
BspANI GGCC 3 cut(s) 5, 110, 118
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 2 cut(s) 151, 502
BspMI ACCTGC 1 cut(s) 314
BspQI GCTCTTC 1 cut(s) 165
BspTI CTTAAG 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 153, 342
BsrI ACTGG 3 cut(s) 369, 389, 502
BssECI CCNNGG 2 cut(s) 28, 119
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 28
Bst4CI ACNGT 2 cut(s) 37, 469
Bst6I CTCTTC 1 cut(s) 165
BstAFI CTTAAG 1 cut(s) 50
BstDEI CTNAG 2 cut(s) 105, 477
BstF5I GGATG 1 cut(s) 269
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 1 cut(s) 238
BstMBI GATC 1 cut(s) 46
BstMWI GCNNNNNNNGC 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 359
BsuRI GGCC 3 cut(s) 5, 110, 118
BtsCI GGATG 1 cut(s) 269
BveI ACCTGC 1 cut(s) 314
Csp6I GTAC 3 cut(s) 25, 362, 465
CviAII CATG 2 cut(s) 94, 409
CviQI GTAC 3 cut(s) 25, 362, 465
DdeI CTNAG 2 cut(s) 105, 477
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 28
EcoT14I CCWWGG 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 28
FaeI CATG 2 cut(s) 97, 412
FatI CATG 2 cut(s) 93, 408
Fnu4HI GCNGC 1 cut(s) 348
FokI GGATG 1 cut(s) 276
Fsp4HI GCNGC 1 cut(s) 348
FspBI CTAG 2 cut(s) 29, 134
GluI GCNGC 1 cut(s) 348
GsaI CCCAGC 2 cut(s) 310, 361
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 3 cut(s) 5, 110, 118
HapII CCGG 1 cut(s) 119
Hin1II CATG 2 cut(s) 97, 412
HincII GTYRAC 2 cut(s) 189, 259
HindII GTYRAC 2 cut(s) 189, 259
HinfI GANTC 6 cut(s) 222, 246, 274, 481, 514, 524
HpaI GTTAAC 1 cut(s) 259
HpaII CCGG 1 cut(s) 119
Hpy166II GTNNAC 3 cut(s) 25, 189, 259
Hpy188I TCNGA 1 cut(s) 486
Hpy188III TCNNGA 2 cut(s) 271, 534
Hpy8I GTNNAC 3 cut(s) 25, 189, 259
HpyAV CCTTC 2 cut(s) 108, 439
HpyCH4III ACNGT 2 cut(s) 37, 469
HpyCH4V TGCA 5 cut(s) 77, 89, 323, 347, 461
HpyF10VI GCNNNNNNNGC 1 cut(s) 347
HpyF3I CTNAG 2 cut(s) 105, 477
Hsp92II CATG 2 cut(s) 97, 412
KspAI GTTAAC 1 cut(s) 259
Kzo9I GATC 1 cut(s) 46
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 2 cut(s) 12, 500
Lsp1109I GCAGC 1 cut(s) 359
MaeI CTAG 2 cut(s) 29, 134
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 5 cut(s) 152, 309, 417, 424, 427
MhlI GDGCHC 1 cut(s) 17
MluCI AATT 3 cut(s) 144, 182, 509
MlyI GAGTC 2 cut(s) 240, 508
MnlI CCTC 2 cut(s) 34, 530
MseI TTAA 4 cut(s) 51, 230, 258, 282
MslI CAYNNNNRTG 1 cut(s) 20
MspCI CTTAAG 1 cut(s) 50
MspI CCGG 1 cut(s) 119
MspR9I CCNGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 347
NciI CCSGG 1 cut(s) 120
NdeII GATC 1 cut(s) 46
NlaIII CATG 2 cut(s) 97, 412
NlaIV GGNNCC 2 cut(s) 151, 502
OliI CACNNNNGTG 1 cut(s) 20
PciSI GCTCTTC 1 cut(s) 165
PfeI GAWTC 4 cut(s) 222, 274, 481, 524
PflMI CCANNNNNTGG 1 cut(s) 497
PkrI GCNGC 1 cut(s) 349
PleI GAGTC 2 cut(s) 240, 508
PpsI GAGTC 2 cut(s) 240, 508
PspFI CCCAGC 2 cut(s) 306, 357
PspN4I GGNNCC 2 cut(s) 151, 502
RsaI GTAC 3 cut(s) 26, 363, 466
RsaNI GTAC 3 cut(s) 25, 362, 465
RseI CAYNNNNRTG 1 cut(s) 20
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 4 cut(s) 51, 230, 258, 282
SatI GCNGC 1 cut(s) 348
Sau3AI GATC 1 cut(s) 46
SchI GAGTC 2 cut(s) 240, 508
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 1 cut(s) 17
SetI ASST 7 cut(s) 30, 203, 256, 328, 359, 383, 541
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 3 cut(s) 50, 239, 269
SmoI CTYRAG 3 cut(s) 50, 239, 269
Sse9I AATT 3 cut(s) 144, 182, 509
SspI AATATT 1 cut(s) 127
SspMI CTAG 2 cut(s) 29, 134
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 37, 469
TaqI TCGA 2 cut(s) 215, 517
TasI AATT 3 cut(s) 144, 182, 509
TfiI GAWTC 4 cut(s) 222, 274, 481, 524
Tru1I TTAA 4 cut(s) 51, 230, 258, 282
Tru9I TTAA 4 cut(s) 51, 230, 258, 282
TseI GCWGC 1 cut(s) 347
TspDTI ATGAA 4 cut(s) 198, 390, 516, 522
TspGWI ACGGA 1 cut(s) 276
Van91I CCANNNNNTGG 1 cut(s) 497
Vha464I CTTAAG 1 cut(s) 50
XmaJI CCTAGG 1 cut(s) 28
XspI CTAG 2 cut(s) 29, 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.