pycom07g13140

Protein of unknown function (DUF3522)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0024381
Physical Location & Seq
Forward (+)
14726827 .. 14727653
827 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13140.3

Sequence Viewer

Length: 453 bp
ATGGCTTATACTAAGGCCACAAGGCCGGCCAATATTATTCTTGTAATGGTAATTGGCGCCGCGGCTCTTCTTATTGGGTGGCTGATAGAATTGTCAACCAGGCTTAGGTCATTTTCCTTTTCGACCGGATTCTCCTTAAATATGATTGAGAGGTTGCAAGCTGTTAAGGGATGGCTCAAGAACCTCATTAAGACAATTATTACAAGATTTCGCTGGGGTTTTGTACTGGCAGGTTTAGTTGCATTAGCCCTTGCAGCAAGTAGCTGGGTACTGGAGACCAGTGAAAGCTACTGGATTTGGCACAGCATTTGGCACATAACAATATATACATCTTCCTTCTTCTTCCTATGCTCGAAAGCAAGTCCTGTAGCTACGGTAGAGACTGAGAATCAAATGCCACCAAATGGAGCCTATGAATTGACTCGACAAGATTCATTTTCAAGAGGCCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3522

Protein Analysis

151

Amino Acids

16.9

Weight (kDa)

9.72

Isoelectric Point (pI)

53.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024381)

Species Orthologous Gene IDs
malus_domestica MD01G1067000.v1.1
pyrus_communis pycom07g13140
rosa_multiflora Rmu_sc0004814.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 221
AccB1I GGYRCC 1 cut(s) 56
AccB7I CCANNNNNTGG 1 cut(s) 404
AccII CGCG 1 cut(s) 62
AciI CCGC 2 cut(s) 60, 62
AcoI YGGCCR 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 225, 270
AfiI CCNNNNNNNGG 2 cut(s) 105, 404
AgsI TTSAA 1 cut(s) 441
AjnI CCWGG 1 cut(s) 98
AluBI AGCT 4 cut(s) 161, 264, 288, 371
AluI AGCT 4 cut(s) 161, 264, 288, 371
Alw26I GTCTC 2 cut(s) 269, 374
AoxI GGCC 4 cut(s) 15, 23, 27, 445
ApeKI GCWGC 1 cut(s) 254
AspLEI GCGC 1 cut(s) 59
BanI GGYRCC 1 cut(s) 56
BbvI GCAGC 1 cut(s) 266
BccI CCATC 1 cut(s) 165
BciT130I CCWGG 1 cut(s) 100
BcoDI GTCTC 2 cut(s) 269, 374
BfmI CTRYAG 1 cut(s) 366
BfoI RGCGCY 1 cut(s) 60
BfuAI ACCTGC 1 cut(s) 221
BisI GCNGC 3 cut(s) 60, 63, 255
BlsI GCNGC 3 cut(s) 61, 64, 256
Bme1390I CCNGG 1 cut(s) 100
BmiI GGNNCC 2 cut(s) 58, 409
BmrFI CCNGG 1 cut(s) 100
BpmI CTGGAG 1 cut(s) 293
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 60
BsaWI WCCGGW 1 cut(s) 125
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 404
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 4 cut(s) 231, 276, 279, 296
BseBI CCWGG 1 cut(s) 100
BseDI CCNNGG 1 cut(s) 60
BseGI GGATG 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 105, 404
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 4 cut(s) 231, 276, 279, 296
BseXI GCAGC 1 cut(s) 266
BseYI CCCAGC 2 cut(s) 213, 264
Bsh1236I CGCG 1 cut(s) 62
Bsh1285I CGRYCG 1 cut(s) 126
BshFI GGCC 4 cut(s) 17, 25, 29, 447
BshNI GGYRCC 1 cut(s) 56
BsiEI CGRYCG 1 cut(s) 126
BsiSI CCGG 2 cut(s) 26, 126
BslI CCNNNNNNNGG 2 cut(s) 105, 404
BsmAI GTCTC 2 cut(s) 269, 374
BsnI GGCC 4 cut(s) 17, 25, 29, 447
Bso31I GGTCTC 1 cut(s) 269
BspACI CCGC 2 cut(s) 60, 62
BspANI GGCC 4 cut(s) 17, 25, 29, 447
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 62
BspLI GGNNCC 2 cut(s) 58, 409
BspMI ACCTGC 1 cut(s) 221
BspQI GCTCTTC 1 cut(s) 72
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 4 cut(s) 231, 276, 279, 296
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 1 cut(s) 60
BssNI GRCGYC 1 cut(s) 57
Bst2UI CCWGG 1 cut(s) 100
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 1 cut(s) 72
BstACI GRCGYC 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 27, 159
BstDEI CTNAG 3 cut(s) 12, 104, 384
BstDSI CCRYGG 1 cut(s) 60
BstF5I GGATG 1 cut(s) 176
BstFNI CGCG 1 cut(s) 62
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 1 cut(s) 59
BstMAI GTCTC 2 cut(s) 269, 374
BstMCI CGRYCG 1 cut(s) 126
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 100
BstSCI CCNGG 1 cut(s) 98
BstSFI CTRYAG 1 cut(s) 366
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 1 cut(s) 266
BsuRI GGCC 4 cut(s) 17, 25, 29, 447
BtgI CCRYGG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 176
BtsIMutI CAGTG 1 cut(s) 286
BveI ACCTGC 1 cut(s) 221
Cac8I GCNNGC 2 cut(s) 27, 159
CfoI GCGC 1 cut(s) 59
Cfr10I RCCGGY 1 cut(s) 25
Cfr42I CCGCGG 1 cut(s) 63
Csp6I GTAC 2 cut(s) 224, 269
CviQI GTAC 2 cut(s) 224, 269
DdeI CTNAG 3 cut(s) 12, 104, 384
DinI GGCGCC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 72
EarI CTCTTC 1 cut(s) 72
Eco31I GGTCTC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 98
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
FaiI YATR 7 cut(s) 9, 143, 317, 325, 327, 349, 414
Fnu4HI GCNGC 3 cut(s) 60, 63, 255
FokI GGATG 1 cut(s) 183
FseI GGCCGGCC 1 cut(s) 29
Fsp4HI GCNGC 3 cut(s) 60, 63, 255
GlaI GCGC 1 cut(s) 58
GluI GCNGC 3 cut(s) 60, 63, 255
GsaI CCCAGC 2 cut(s) 217, 268
GsuI CTGGAG 1 cut(s) 293
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 4 cut(s) 17, 25, 29, 447
HapII CCGG 2 cut(s) 26, 126
HhaI GCGC 1 cut(s) 59
Hin1I GRCGYC 1 cut(s) 57
Hin6I GCGC 1 cut(s) 57
HinP1I GCGC 1 cut(s) 57
HincII GTYRAC 1 cut(s) 96
HindII GTYRAC 1 cut(s) 96
HinfI GANTC 4 cut(s) 129, 388, 421, 431
HpaII CCGG 2 cut(s) 26, 126
Hpy166II GTNNAC 1 cut(s) 96
Hpy188III TCNNGA 2 cut(s) 178, 441
Hpy8I GTNNAC 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4V TGCA 3 cut(s) 157, 242, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 3 cut(s) 12, 104, 384
Hsp92I GRCGYC 1 cut(s) 57
HspAI GCGC 1 cut(s) 57
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 25
KroNI GCCGGC 1 cut(s) 27
KspI CCGCGG 1 cut(s) 63
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 1 cut(s) 407
Lsp1109I GCAGC 1 cut(s) 266
MboII GAAGA 4 cut(s) 59, 324, 331, 334
MluCI AATT 4 cut(s) 51, 89, 195, 416
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 415
MnlI CCTC 3 cut(s) 144, 194, 437
MroNI GCCGGC 1 cut(s) 25
MseI TTAA 3 cut(s) 137, 165, 189
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 2 cut(s) 26, 126
MspR9I CCNGG 1 cut(s) 100
MvaI CCWGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 1 cut(s) 254
NaeI GCCGGC 1 cut(s) 27
NarI GGCGCC 1 cut(s) 57
NgoMIV GCCGGC 1 cut(s) 25
NlaIV GGNNCC 2 cut(s) 58, 409
PciSI GCTCTTC 1 cut(s) 72
PdiI GCCGGC 1 cut(s) 27
PfeI GAWTC 3 cut(s) 129, 388, 431
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 3 cut(s) 61, 64, 256
PleI GAGTC 1 cut(s) 415
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 98
PspFI CCCAGC 2 cut(s) 213, 264
PspGI CCWGG 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 58, 409
RigI GGCCGGCC 1 cut(s) 29
RsaI GTAC 2 cut(s) 225, 270
RsaNI GTAC 2 cut(s) 224, 269
SacII CCGCGG 1 cut(s) 63
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 3 cut(s) 137, 165, 189
SatI GCNGC 3 cut(s) 60, 63, 255
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 100
SetI ASST 8 cut(s) 110, 155, 163, 186, 235, 266, 290, 373
SfcI CTRYAG 1 cut(s) 366
SfoI GGCGCC 1 cut(s) 58
Sfr303I CCGCGG 1 cut(s) 63
SgrBI CCGCGG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 4 cut(s) 51, 89, 195, 416
SsiI CCGC 2 cut(s) 60, 62
SspDI GGCGCC 1 cut(s) 56
SspI AATATT 1 cut(s) 34
StyD4I CCNGG 1 cut(s) 98
TaaI ACNGT 1 cut(s) 376
TaqI TCGA 3 cut(s) 122, 353, 424
TasI AATT 4 cut(s) 51, 89, 195, 416
TatI WGTACW 1 cut(s) 223
TauI GCSGC 2 cut(s) 62, 65
TfiI GAWTC 3 cut(s) 129, 388, 431
Tru1I TTAA 3 cut(s) 137, 165, 189
Tru9I TTAA 3 cut(s) 137, 165, 189
TscAI CASTG 1 cut(s) 286
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 423, 429
TspRI CASTG 1 cut(s) 286
Van91I CCANNNNNTGG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.