MD01G1102300.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0020382
Physical Location & Seq
Forward (+)
21472582 .. 21475079
2498 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1102300.v1.1.491

Sequence Viewer

Length: 909 bp
ATGCTGAAGAAAGTCAACTTGATGTCTCCTCGACAAAGTGAAGATATACAGCTTCCACCTCGTACAAAGCCCATTAAGGATCTACGTGTATCCCCATGTGGTAGATTCGCCCTTTTAGCTTCACTGGGGAAGAAACTATCATTTCTTAGCTTGGGGAGCAACAGTTTTGTCACCACTTATGACTTACCGGGCCCCGCATGGTCGTGCTCATGGGATCTTAACACGCCACATTGTATATATACTGGTTTACAGGATGGTATGCTTTTAATGTTCGATATGCGTTTTAGCAAGGTTCCTCTGCTATCTTTGGCTGGGCTGACCTCCCGGCCAATTCATACTATACACTCCCTCGTGCAAAACCGTGCTTTCAGTCACACTTCTCATAAATTGCTCACTGCTTCTTCCATTGGCCCTTGCGTGTGGAACATGGGTAGTACTGGTCCTGCTGGAGAAAGGCCATTTCTCATCCCCGGATTAGAGGATCAAGGTGTTTGTATATCAGTAGCTTATAGTCCCTCAACTGATAACATAGTTGCTTCATATCATCCGAAAACCAATACATCCGCTAGACCTTCTGGTTCACAAGTTCTTATCAAGAGGGTAGATGATTGCTTTTATCATAAGATGGGATCTGTGCCAGTTCATTTGAATGACTTTCAGCTGAGAAAGTCTGCCATTGTAGATATACCAGACTGCTACCCATTGTTTGCGTATGGTGAGGAACTGACCCGTGGATTGAGGCTGCGGGAATTGCCAAGTCTAAAAGTGAGCCAGAATCTTGAACCTCATCAACATTCCATCCTTGACGTGAAGTATGCACGTTCTCAGGACAAAGGTTTGCTTGGTTGCTTGAGTGAGGACAAGTTGCAACTTTTCTCCGCGGCTACAAAAGGTGTGTTTATATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

303

Amino Acids

33.33

Weight (kDa)

9.07

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RFWD3 PF23419 3 - 292 3.4e-24 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020382)

Species Orthologous Gene IDs
malus_domestica MD01G1102300.v1.1
prunus_persica Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1 Prupe.2G207900_v2.0.a1
pyrus_communis pycom01g12870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 881
AciI CCGC 5 cut(s) 195, 564, 745, 879, 881
AclWI GGATC 4 cut(s) 87, 222, 489, 637
AcoI YGGCCR 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 26
AfaI GTAC 2 cut(s) 64, 436
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 2 cut(s) 649, 782
AjiI CACGTC 1 cut(s) 808
AluBI AGCT 5 cut(s) 52, 119, 150, 506, 661
AluI AGCT 5 cut(s) 52, 119, 150, 506, 661
Alw21I GWGCWC 1 cut(s) 209
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 4 cut(s) 87, 222, 489, 637
AoxI GGCC 4 cut(s) 190, 326, 409, 455
ApaI GGGCCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 742
AspS9I GGNCC 4 cut(s) 190, 191, 410, 440
AsuC2I CCSGG 3 cut(s) 189, 325, 471
AsuHPI GGTGA 2 cut(s) 163, 728
AvaII GGWCC 1 cut(s) 440
BaeGI GKGCMC 1 cut(s) 194
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 209
BbvI GCAGC 1 cut(s) 729
BccI CCATC 3 cut(s) 248, 619, 806
BciVI GTATCC 1 cut(s) 100
BcnI CCSGG 3 cut(s) 189, 325, 471
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 567
BfuI GTATCC 1 cut(s) 100
BisI GCNGC 2 cut(s) 743, 882
BlsI GCNGC 2 cut(s) 744, 883
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 3 cut(s) 189, 325, 471
Bme18I GGWCC 1 cut(s) 440
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 4 cut(s) 190, 191, 410, 440
BmiI GGNNCC 3 cut(s) 192, 193, 294
BmrFI CCNGG 3 cut(s) 189, 325, 471
BmrI ACTGGG 1 cut(s) 134
BmuI ACTGGG 1 cut(s) 134
BpmI CTGGAG 1 cut(s) 468
BpuEI CTTGAG 1 cut(s) 871
BpuMI CCSGG 3 cut(s) 189, 325, 471
BsaAI YACGTR 1 cut(s) 86
BsaJI CCNNGG 3 cut(s) 469, 730, 879
BsaXI ACNNNNNCTCC 2 cut(s) 148, 178
Bse1I ACTGG 4 cut(s) 129, 247, 442, 638
BseDI CCNNGG 3 cut(s) 469, 730, 879
BseGI GGATG 5 cut(s) 259, 465, 544, 560, 798
BseMII CTCAG 2 cut(s) 653, 839
BseNI ACTGG 4 cut(s) 129, 247, 442, 638
BseRI GAGGAG 1 cut(s) 18
BseSI GKGCMC 1 cut(s) 194
BseXI GCAGC 1 cut(s) 729
BseYI CCCAGC 1 cut(s) 311
Bsh1236I CGCG 1 cut(s) 881
BshFI GGCC 4 cut(s) 192, 328, 411, 457
BsiHKAI GWGCWC 1 cut(s) 209
BsiSI CCGG 3 cut(s) 188, 325, 471
BslFI GGGAC 1 cut(s) 498
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 498
BsnI GGCC 4 cut(s) 192, 328, 411, 457
Bsp120I GGGCCC 1 cut(s) 190
Bsp1286I GDGCHC 2 cut(s) 194, 209
Bsp143I GATC 4 cut(s) 79, 214, 481, 629
BspACI CCGC 5 cut(s) 195, 564, 745, 879, 881
BspANI GGCC 4 cut(s) 192, 328, 411, 457
BspCNI CTCAG 2 cut(s) 654, 838
BspFNI CGCG 1 cut(s) 881
BspLI GGNNCC 3 cut(s) 192, 193, 294
BspPI GGATC 4 cut(s) 87, 222, 489, 637
BsrI ACTGG 4 cut(s) 129, 247, 442, 638
BssECI CCNNGG 3 cut(s) 469, 730, 879
BssMI GATC 4 cut(s) 79, 214, 481, 629
BssSI CACGAG 1 cut(s) 350
Bst2BI CACGAG 1 cut(s) 350
Bst4CI ACNGT 2 cut(s) 164, 362
BstBAI YACGTR 1 cut(s) 86
BstDEI CTNAG 3 cut(s) 146, 662, 825
BstDSI CCRYGG 2 cut(s) 730, 879
BstF5I GGATG 5 cut(s) 259, 465, 544, 560, 798
BstFNI CGCG 1 cut(s) 881
BstKTI GATC 4 cut(s) 82, 217, 484, 632
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 4 cut(s) 79, 214, 481, 629
BstMWI GCNNNNNNNGC 3 cut(s) 116, 156, 751
BstSCI CCNGG 3 cut(s) 187, 323, 469
BstSLI GKGCMC 1 cut(s) 194
BstUI CGCG 1 cut(s) 881
BstV1I GCAGC 1 cut(s) 729
BstX2I RGATCY 3 cut(s) 79, 214, 629
BstYI RGATCY 3 cut(s) 79, 214, 629
BsuI GTATCC 1 cut(s) 100
BsuRI GGCC 4 cut(s) 192, 328, 411, 457
BtgI CCRYGG 2 cut(s) 730, 879
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 5 cut(s) 259, 465, 544, 560, 798
BtsI GCAGTG 1 cut(s) 393
BtsIMutI CAGTG 2 cut(s) 122, 393
Cfr13I GGNCC 4 cut(s) 190, 191, 410, 440
Cfr42I CCGCGG 1 cut(s) 882
Csp6I GTAC 2 cut(s) 63, 435
CviAII CATG 4 cut(s) 96, 198, 210, 427
CviQI GTAC 2 cut(s) 63, 435
DdeI CTNAG 3 cut(s) 146, 662, 825
DpnI GATC 4 cut(s) 81, 216, 483, 631
DpnII GATC 4 cut(s) 79, 214, 481, 629
EaeI YGGCCR 1 cut(s) 326
Eco24I GRGCYC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 440
Eco57I CTGAAG 1 cut(s) 26
EcoO109I RGGNCCY 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 194
FaeI CATG 4 cut(s) 99, 201, 213, 430
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 1 cut(s) 498
FatI CATG 4 cut(s) 95, 197, 209, 426
FauI CCCGC 2 cut(s) 202, 738
Fnu4HI GCNGC 2 cut(s) 743, 882
FokI GGATG 5 cut(s) 266, 452, 531, 547, 785
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 2 cut(s) 743, 882
FspBI CTAG 1 cut(s) 567
GluI GCNGC 2 cut(s) 743, 882
GsaI CCCAGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 468
HaeIII GGCC 4 cut(s) 192, 328, 411, 457
HapII CCGG 3 cut(s) 188, 325, 471
Hin1II CATG 4 cut(s) 99, 201, 213, 430
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 2 cut(s) 105, 775
HpaII CCGG 3 cut(s) 188, 325, 471
HphI GGTGA 2 cut(s) 163, 728
Hpy166II GTNNAC 3 cut(s) 16, 248, 581
Hpy188I TCNGA 1 cut(s) 549
Hpy188III TCNNGA 3 cut(s) 595, 779, 827
Hpy8I GTNNAC 3 cut(s) 16, 248, 581
HpyAV CCTTC 1 cut(s) 582
HpyCH4III ACNGT 2 cut(s) 164, 362
HpyCH4IV ACGT 3 cut(s) 85, 807, 820
HpyCH4V TGCA 3 cut(s) 355, 818, 868
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 156, 751
HpyF3I CTNAG 3 cut(s) 146, 662, 825
HpySE526I ACGT 3 cut(s) 85, 807, 820
Hsp92II CATG 4 cut(s) 99, 201, 213, 430
KspI CCGCGG 1 cut(s) 882
Kzo9I GATC 4 cut(s) 79, 214, 481, 629
LmnI GCTCC 1 cut(s) 156
Lsp1109I GCAGC 1 cut(s) 729
MaeI CTAG 1 cut(s) 567
MaeII ACGT 3 cut(s) 85, 807, 820
MaeIII GTNAC 2 cut(s) 169, 371
MalI GATC 4 cut(s) 81, 216, 483, 631
MboI GATC 4 cut(s) 79, 214, 481, 629
MboII GAAGA 4 cut(s) 19, 53, 142, 393
MflI RGATCY 3 cut(s) 79, 214, 629
MhlI GDGCHC 2 cut(s) 194, 209
MluCI AATT 3 cut(s) 330, 386, 749
MseI TTAA 3 cut(s) 75, 219, 266
MslI CAYNNNNRTG 2 cut(s) 202, 648
MspA1I CMGCKG 2 cut(s) 661, 881
MspI CCGG 3 cut(s) 188, 325, 471
MspR9I CCNGG 3 cut(s) 189, 325, 471
MvnI CGCG 1 cut(s) 881
MwoI GCNNNNNNNGC 3 cut(s) 116, 156, 751
NciI CCSGG 3 cut(s) 189, 325, 471
NdeII GATC 4 cut(s) 79, 214, 481, 629
NlaIII CATG 4 cut(s) 99, 201, 213, 430
NlaIV GGNNCC 3 cut(s) 192, 193, 294
NmuCI GTSAC 2 cut(s) 169, 371
PfeI GAWTC 2 cut(s) 105, 775
PkrI GCNGC 2 cut(s) 744, 883
Ppu21I YACGTR 1 cut(s) 86
PspFI CCCAGC 1 cut(s) 311
PspN4I GGNNCC 3 cut(s) 192, 193, 294
PspOMI GGGCCC 1 cut(s) 190
PspPI GGNCC 4 cut(s) 190, 191, 410, 440
PsuI RGATCY 3 cut(s) 79, 214, 629
PvuII CAGCTG 1 cut(s) 661
RsaI GTAC 2 cut(s) 64, 436
RsaNI GTAC 2 cut(s) 63, 435
RseI CAYNNNNRTG 2 cut(s) 202, 648
SacII CCGCGG 1 cut(s) 882
SaqAI TTAA 3 cut(s) 75, 219, 266
SatI GCNGC 2 cut(s) 743, 882
Sau3AI GATC 4 cut(s) 79, 214, 481, 629
Sau96I GGNCC 4 cut(s) 190, 191, 410, 440
ScaI AGTACT 1 cut(s) 436
ScrFI CCNGG 3 cut(s) 189, 325, 471
SduI GDGCHC 2 cut(s) 194, 209
Sfr303I CCGCGG 1 cut(s) 882
SgrBI CCGCGG 1 cut(s) 882
SinI GGWCC 1 cut(s) 440
SmiMI CAYNNNNRTG 2 cut(s) 202, 648
SmlI CTYRAG 1 cut(s) 850
SmoI CTYRAG 1 cut(s) 850
Sse9I AATT 3 cut(s) 330, 386, 749
SsiI CCGC 5 cut(s) 195, 564, 745, 879, 881
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 3 cut(s) 187, 323, 469
TaaI ACNGT 2 cut(s) 164, 362
TaiI ACGT 3 cut(s) 88, 810, 823
TaqI TCGA 2 cut(s) 31, 273
TasI AATT 3 cut(s) 330, 386, 749
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 2 cut(s) 105, 775
Tru1I TTAA 3 cut(s) 75, 219, 266
Tru9I TTAA 3 cut(s) 75, 219, 266
TscAI CASTG 2 cut(s) 129, 400
TseFI GTSAC 2 cut(s) 169, 371
TseI GCWGC 1 cut(s) 742
Tsp45I GTSAC 2 cut(s) 169, 371
TspDTI ATGAA 3 cut(s) 323, 528, 632
TspRI CASTG 2 cut(s) 129, 400
VpaK11BI GGWCC 1 cut(s) 440
XspI CTAG 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.