Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008268

Physical Location & Seq

Genomic Coordinates Forward (+)

22056014 .. 22063013

Gene Structure 7000 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1107900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGGGTATCTCGCAGAATGATGGTAAAATGGAAGGTTGGCTTTATATAATCGGGTCCAATCGATTTGGGCTGCAATACTCGCGCAAAAGGTATTTTATTCTCAAGGATAATTGCCTCAGGAGCTTCAAAGCCAAACCTTCTTCTGACTCGGAGGAACCAGTTAGAAGTGCAGTAATAGATACCTGCATTCGGGCCACGGATAATGGAAGGGCAAGCATTCATAGAAAAGGCTTCTTTGTTTTCACGCTCTACAATACTTCCAATAACAGCGATCAGCTCAAGTTGGGAGCAAGTAGTTCAGAAGAAGCAGCAAAATGGATTCGTTCTTTGCAGGATGCTGCATTAAAGGCCTGCCCAAATGCAACACAGGATTTTGTGGCTTGTTCTAAGAGCAGATGGCCATCTTTTAGACTGGTTGGTTCCAGGAGGACAGAACGTAAAAACTCCGTTGACTGGACTTTTAGCCCATTGATGCACACAGAAGGAATGACATCTGATGTTATTGCTCCCTCACCGTGGAAAATTTTTGGTTGTCAGAATGGACTACGGCTTTTCAAAGAAGCAAAAGATTGGGATTCCCGTGGAAGGCATTGGGATGATCATCCGGCTATAATGGCAGTAGGAGTGGTTGATGGAACTTCAGAGGCCATTTTCCGGACTCTTCTATCGCTTGGCCCCTCAAGATCAGAATGGGATTTCTGTTACTACCAGGGTAGCGTGGTTGAGAGGCTTGATGGTCATACAGATATTATTCACCAGCAGTTATACAGTGATTGGCTACCCTGGGGGATGCAACGAAGAGATCTACTGATACGTCGCTATTGGAGAAGGGAGGATGATGGCACATATGTGATCCTCTACCATTCAGTGTCTCACAAGAAATGTCCTCCAAAAAGAGGCTATGTCCGTGCTTGCCTTAAAAGCGGAGGATTTGTTATAACTCCTGCAAACCAAGGGAAACAATCTCTTGTGAGACATATGCTTGCTATTGATTGGAAATTCTGGAAATTGTATCTGCGTCCATCATCTGCAAGATGCATCACCATCCGTATGCTTGAGAGAGTTGCTGCATTAAGAGAGCTGTTCGGAGCCAAAGCTGGAACTAGCTCCCCTGAATTCTCATCCGGAGACATGTCAAGTGAGATCAAATTTTCTCAAAGTGGACAGCAAAATATCAAGACTGAAGTTCAGCACCCTGGAGAAGTCAAAAAGATGGAGGAGGGTGTTCTCGTGGAAGATGAGGTGGAAAAGCCAGAAGGTCGCGCAAGTTTAAAGGGGCTGCACGATGCTGCAGACGAGTTCTTTGATGTTCCAGAACCAACAGAGTATGACCAGTTCGAGAACGAGTGGCCTTCAGATGTGAGCATGGAACAGAATCCCACGAGTATACAACATCACAAACTGTCATCAGCTGCCGTTCTTGTGAAGAAATTGCATGAACTTAGAGTTCAAAAGAAGGGTTATATGGATTTACAAGAGGTGTGTAAGGAAGACAGTGTATGCCCCTATGGGGCAACACTTCAAAAGGACCCAAGTTGTGCTTTACCTTGCAGTTGGGGATCAGCCGATCCTTCTTCATTTTTGATACGCGGGCCAAATTATTTACGAGATCGTCAAAAGATCAAGGCAAAAAGCACCTTGATGCAACTGGTTGGTGTAGATTGGCTAAGATCTGACAAGCGAGAAGATGATCTTGGCAGCCGTTATGGTAATATTGTTCAGAAATCTGCAGAACGAGGTGGATCAGAGTTTTTCTTTATTGTTAACATACAAGTGCCCGGAACAACCATGTATACCTTGGCGTTTTATTACATGATAAAAACTCCTCTGGAGGAAACTCCTTTGCTACATGCCTTTGTCAATGGAGATGATTCCTATAGGAATTCAAGATTTAAGCTAATACCGTACATTTCTAAGGGGTCATGGATAGTCAAGCAGAGTGTAGGGAAGAAAGCCTGCTTGGTAGGGCAAGCGCTAGAAGTGCATTATTTCCGCGGGAAGAACTATCTTGAGCTTGGGGTTGACGTCGGGTCATCAACGGTGGCAAGGGGGGTGGTAAATCTTGTTCTTGGTTACCTCAACAATTTGGTCATAGAAATGGCATTTCTGATACAGGCCAACACAGAAGAAGAACTGCCAGAAGTGCTTCTTGGAACATGCAGAATTAACCAACTGGATGCATCGAAATCCGTCCTGGTAAAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF1336 PH START

Protein Analysis

736

Amino Acids

83.18

Weight (kDa)

8.67

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PH	PF00169	9 - 113	7.6e-13	PH domain
START	PF01852	202 - 345	5.1e-18	START domain
EDR2_C	PF07059	519 - 726	9.4e-77	Protein ENHANCED DISEASE RESISTANCE 2, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54800 AT3G54800
fragaria_vesca	FvH4_7g19330 FvH4_7g19330 FvH4_7g19330 FvH4_7g19330
malus_domestica	MD01G1107900.v1.1 MD07G1173600.v1.1
prunus_persica	Prupe.2G212800_v2.0.a1 Prupe.2G212800_v2.0.a1 Prupe.2G212800_v2.0.a1
pyrus_communis	pycom01g13480 pycom07g16810
rosa_chinensis	RchiOBHm_Chr1g0363211
rosa_laevigata	RLG00000027583
rosa_multiflora	Rmu_sc0001770.1_g000002
rosa_roxburghii	Rroxscaffold_4G00293360
rosa_rugosa	Rorug01G0305600
rosa_samantha	Rh1AG314300 Rh1BG277800 Rh1CG294200 Rh1DG308500
rosa_wichuraiana	Rw1G027850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	938
AatII	GACGTC	1 cut(s)	2028
Acc36I	ACCTGC	1 cut(s)	191
AccI	GTMKAC	2 cut(s)	1387, 1793
AccII	CGCG	4 cut(s)	82, 1263, 1590, 1995
AccIII	TCCGGA	2 cut(s)	654, 1124
AciI	CCGC	4 cut(s)	924, 1590, 1993, 1995
AclWI	GGATC	4 cut(s)	847, 1562, 1567, 1750
AcoI	YGGCCR	1 cut(s)	398
AcsI	RAATTY	5 cut(s)	522, 998, 1115, 1148, 1882
AcuI	CTGAAG	3 cut(s)	624, 1203, 1338
AcyI	GRCGYC	1 cut(s)	2025
AfaI	GTAC	1 cut(s)	1907
AfeI	AGCGCT	1 cut(s)	1974
AfiI	CCNNNNNNNGG	7 cut(s)	189, 453, 516, 585, 654, 896, 1510
AflIII	ACRYGT	1 cut(s)	1131
AgsI	TTSAA	5 cut(s)	127, 556, 1451, 1523, 1887
AhdI	GACNNNNNGTC	1 cut(s)	2029
AjnI	CCWGG	5 cut(s)	422, 708, 782, 1195, 2193
AjuI	GAANNNNNNNTTGG	6 cut(s)	1677, 1709, 1943, 1975, 2133, 2165
AleI	CACNNNNGTG	1 cut(s)	848
AluBI	AGCT	8 cut(s)	123, 277, 1081, 1097, 1107, 1412, 1897, 2014
AluI	AGCT	8 cut(s)	123, 277, 1081, 1097, 1107, 1412, 1897, 2014
Alw26I	GTCTC	3 cut(s)	876, 967, 1122
AlwI	GGATC	4 cut(s)	847, 1562, 1567, 1750
Aor13HI	TCCGGA	2 cut(s)	654, 1124
Aor51HI	AGCGCT	1 cut(s)	1974
AoxI	GGCC	8 cut(s)	192, 348, 398, 645, 673, 1349, 1592, 2115
ApeKI	GCWGC	8 cut(s)	70, 308, 338, 1067, 1279, 1289, 1412, 1698
ApoI	RAATTY	5 cut(s)	522, 998, 1115, 1148, 1882
ArsI	GACNNNNNNTTYG	2 cut(s)	1286, 1318
Asp700I	GAANNNNTTC	1 cut(s)	2145
AspLEI	GCGC	3 cut(s)	84, 1265, 1975
AspS9I	GGNCC	5 cut(s)	54, 192, 674, 1528, 1592
AsuC2I	CCSGG	1 cut(s)	1779
AsuHPI	GGTGA	3 cut(s)	504, 746, 1033
AvaII	GGWCC	2 cut(s)	54, 1528
AxyI	CCTNAGG	1 cut(s)	116
BaeGI	GKGCMC	1 cut(s)	1779
BalI	TGGCCA	1 cut(s)	400
BauI	CACGAG	2 cut(s)	1229, 1381
BbsI	GAAGAC	1 cut(s)	1497
BbvI	GCAGC	8 cut(s)	57, 320, 325, 1054, 1266, 1276, 1399, 1710
BccI	CCATC	9 cut(s)	14, 390, 409, 626, 728, 833, 1030, 1052, 1207
BceAI	ACGGC	3 cut(s)	563, 1400, 1686
BciT130I	CCWGG	5 cut(s)	424, 710, 784, 1197, 2195
BclI	TGATCA	1 cut(s)	598
BcnI	CCSGG	1 cut(s)	1779
BcoDI	GTCTC	3 cut(s)	876, 967, 1122
BfaI	CTAG	2 cut(s)	1104, 1976
BfmI	CTRYAG	3 cut(s)	1290, 1728, 1876
BfoI	RGCGCY	1 cut(s)	1976
BfuAI	ACCTGC	1 cut(s)	191
BglII	AGATCT	2 cut(s)	802, 1670
BisI	GCNGC	8 cut(s)	71, 309, 339, 1068, 1280, 1290, 1413, 1699
BlsI	GCNGC	8 cut(s)	72, 310, 340, 1069, 1281, 1291, 1414, 1700
Bme1390I	CCNGG	6 cut(s)	424, 710, 784, 1197, 1779, 2195
Bme18I	GGWCC	2 cut(s)	54, 1528
BmeRI	GACNNNNNGTC	1 cut(s)	2029
BmgT120I	GGNCC	5 cut(s)	54, 192, 674, 1528, 1592
BmiI	GGNNCC	6 cut(s)	55, 156, 421, 676, 1090, 1530
BmrFI	CCNGG	6 cut(s)	424, 710, 784, 1197, 1779, 2195
BmsI	GCATC	9 cut(s)	325, 462, 780, 1025, 1047, 1276, 1632, 2167, 2189
BpiI	GAAGAC	1 cut(s)	1497
BpmI	CTGGAG	2 cut(s)	1218, 1850
BpuEI	CTTGAG	5 cut(s)	86, 263, 664, 1076, 2030
BpuMI	CCSGG	1 cut(s)	1779
Bsa29I	ATCGAT	1 cut(s)	61
BsaBI	GATNNNNATC	2 cut(s)	400, 600
BsaHI	GRCGYC	1 cut(s)	2025
BsaWI	WCCGGW	2 cut(s)	654, 1124
BsaXI	ACNNNNNCTCC	2 cut(s)	918, 948
Bsc4I	CCNNNNNNNGG	7 cut(s)	189, 453, 516, 585, 654, 896, 1510
Bse1I	ACTGG	6 cut(s)	158, 417, 458, 1333, 1653, 2178
Bse21I	CCTNAGG	1 cut(s)	116
Bse8I	GATNNNNATC	2 cut(s)	400, 600
BseAI	TCCGGA	2 cut(s)	654, 1124
BseBI	CCWGG	5 cut(s)	424, 710, 784, 1197, 2195
BseCI	ATCGAT	1 cut(s)	61
BseGI	GGATG	8 cut(s)	340, 601, 601, 795, 841, 1044, 1121, 2182
BseJI	GATNNNNATC	2 cut(s)	400, 600
BseLI	CCNNNNNNNGG	7 cut(s)	189, 453, 516, 585, 654, 896, 1510
BseMII	CTCAG	1 cut(s)	130
BseNI	ACTGG	6 cut(s)	158, 417, 458, 1333, 1653, 2178
BseRI	GAGGAG	2 cut(s)	1232, 1815
BseSI	GKGCMC	1 cut(s)	1779
BseXI	GCAGC	8 cut(s)	57, 320, 325, 1054, 1266, 1276, 1399, 1710
BsgI	GTGCAG	2 cut(s)	189, 1265
Bsh1236I	CGCG	4 cut(s)	82, 1263, 1590, 1995
BshFI	GGCC	8 cut(s)	194, 350, 400, 647, 675, 1351, 1594, 2117
BshVI	ATCGAT	1 cut(s)	61
BsiSI	CCGG	4 cut(s)	605, 655, 1125, 1779
BslI	CCNNNNNNNGG	7 cut(s)	189, 453, 516, 585, 654, 896, 1510
BsmAI	GTCTC	3 cut(s)	876, 967, 1122
BsmI	GAATGC	2 cut(s)	186, 216
BsnI	GGCC	8 cut(s)	194, 350, 400, 647, 675, 1351, 1594, 2117
Bsp1286I	GDGCHC	1 cut(s)	1779
Bsp13I	TCCGGA	2 cut(s)	654, 1124
BspACI	CCGC	4 cut(s)	924, 1590, 1993, 1995
BspANI	GGCC	8 cut(s)	194, 350, 400, 647, 675, 1351, 1594, 2117
BspCNI	CTCAG	1 cut(s)	129
BspDI	ATCGAT	1 cut(s)	61
BspEI	TCCGGA	2 cut(s)	654, 1124
BspFNI	CGCG	4 cut(s)	82, 1263, 1590, 1995
BspLI	GGNNCC	6 cut(s)	55, 156, 421, 676, 1090, 1530
BspMAI	CTGCAG	2 cut(s)	1294, 1732
BspMI	ACCTGC	1 cut(s)	191
BspPI	GGATC	4 cut(s)	847, 1562, 1567, 1750
BsrI	ACTGG	6 cut(s)	158, 417, 458, 1333, 1653, 2178
BssNAI	GTATAC	2 cut(s)	1388, 1794
BssNI	GRCGYC	1 cut(s)	2025
BssSI	CACGAG	2 cut(s)	1229, 1381
BssT1I	CCWWGG	2 cut(s)	952, 1797
Bst1107I	GTATAC	2 cut(s)	1388, 1794
Bst2BI	CACGAG	2 cut(s)	1229, 1381
Bst2UI	CCWGG	5 cut(s)	424, 710, 784, 1197, 2195
Bst4CI	ACNGT	6 cut(s)	516, 770, 1404, 1496, 1905, 2041
Bst6I	CTCTTC	2 cut(s)	666, 793
BstACI	GRCGYC	1 cut(s)	2025
BstC8I	GCNNGC	7 cut(s)	214, 352, 913, 984, 1592, 1957, 1971
BstDEI	CTNAG	5 cut(s)	116, 387, 1442, 1667, 1914
BstDSI	CCRYGG	4 cut(s)	195, 515, 580, 1993
BstEII	GGTNACC	1 cut(s)	2072
BstF5I	GGATG	8 cut(s)	340, 601, 601, 795, 841, 1044, 1121, 2182
BstFNI	CGCG	4 cut(s)	82, 1263, 1590, 1995
BstH2I	RGCGCY	1 cut(s)	1976
BstHHI	GCGC	3 cut(s)	84, 1265, 1975
BstMAI	GTCTC	3 cut(s)	876, 967, 1122
BstMWI	GCNNNNNNNGC	7 cut(s)	79, 120, 347, 614, 921, 1981, 2143
BstNI	CCWGG	5 cut(s)	424, 710, 784, 1197, 2195
BstNSI	RCATGY	3 cut(s)	1135, 1853, 2160
BstPI	GGTNACC	1 cut(s)	2072
BstSCI	CCNGG	6 cut(s)	422, 708, 782, 1195, 1777, 2193
BstSFI	CTRYAG	3 cut(s)	1290, 1728, 1876
BstSLI	GKGCMC	1 cut(s)	1779
BstUI	CGCG	4 cut(s)	82, 1263, 1590, 1995
BstV1I	GCAGC	8 cut(s)	57, 320, 325, 1054, 1266, 1276, 1399, 1710
BstV2I	GAAGAC	1 cut(s)	1497
BstX2I	RGATCY	2 cut(s)	802, 1670
BstYI	RGATCY	2 cut(s)	802, 1670
BstZ17I	GTATAC	2 cut(s)	1388, 1794
Bsu15I	ATCGAT	1 cut(s)	61
Bsu36I	CCTNAGG	1 cut(s)	116
BsuRI	GGCC	8 cut(s)	194, 350, 400, 647, 675, 1351, 1594, 2117
BsuTUI	ATCGAT	1 cut(s)	61
BtgI	CCRYGG	4 cut(s)	195, 515, 580, 1993
BtsCI	GGATG	8 cut(s)	340, 601, 601, 795, 841, 1044, 1121, 2182
BtsIMutI	CAGTG	3 cut(s)	775, 873, 1501
BveI	ACCTGC	1 cut(s)	191
Cac8I	GCNNGC	7 cut(s)	214, 352, 913, 984, 1592, 1957, 1971
CfoI	GCGC	3 cut(s)	84, 1265, 1975
Cfr13I	GGNCC	5 cut(s)	54, 192, 674, 1528, 1592
Cfr42I	CCGCGG	1 cut(s)	1996
ClaI	ATCGAT	1 cut(s)	61
CseI	GACGC	1 cut(s)	1007
Csp6I	GTAC	1 cut(s)	1906
CspCI	CAANNNNNGTGG	2 cut(s)	2034, 2069
CviAII	CATG	9 cut(s)	1132, 1366, 1436, 1789, 1813, 1850, 1923, 2157, 2205
CviQI	GTAC	1 cut(s)	1906
DdeI	CTNAG	5 cut(s)	116, 387, 1442, 1667, 1914
DraI	TTTAAA	1 cut(s)	1272
DriI	GACNNNNNGTC	1 cut(s)	2029
EaeI	YGGCCR	1 cut(s)	398
Eam1104I	CTCTTC	2 cut(s)	666, 793
Eam1105I	GACNNNNNGTC	1 cut(s)	2029
EarI	CTCTTC	2 cut(s)	666, 793
Eco130I	CCWWGG	2 cut(s)	952, 1797
Eco147I	AGGCCT	1 cut(s)	350
Eco47I	GGWCC	2 cut(s)	54, 1528
Eco47III	AGCGCT	1 cut(s)	1974
Eco57I	CTGAAG	3 cut(s)	624, 1203, 1338
Eco81I	CCTNAGG	1 cut(s)	116
Eco91I	GGTNACC	1 cut(s)	2072
EcoO109I	RGGNCCY	1 cut(s)	1528
EcoO65I	GGTNACC	1 cut(s)	2072
EcoRI	GAATTC	2 cut(s)	1115, 1882
EcoRII	CCWGG	5 cut(s)	422, 708, 782, 1195, 2193
EcoT14I	CCWWGG	2 cut(s)	952, 1797
EcoT22I	ATGCAT	2 cut(s)	1040, 2182
ErhI	CCWWGG	2 cut(s)	952, 1797
FaeI	CATG	9 cut(s)	1135, 1369, 1439, 1792, 1816, 1853, 1926, 2160, 2208
FalI	AAGNNNNNCTT	8 cut(s)	24, 56, 1525, 1557, 1677, 1709, 2133, 2165
FatI	CATG	9 cut(s)	1131, 1365, 1435, 1788, 1812, 1849, 1922, 2156, 2204
FauI	CCCGC	2 cut(s)	1583, 1988
FauNDI	CATATG	2 cut(s)	847, 978
FbaI	TGATCA	1 cut(s)	598
FblI	GTMKAC	2 cut(s)	1387, 1793
Fnu4HI	GCNGC	8 cut(s)	71, 309, 339, 1068, 1280, 1290, 1413, 1699
FokI	GGATG	8 cut(s)	347, 588, 608, 802, 848, 1031, 1108, 2189
Fsp4HI	GCNGC	8 cut(s)	71, 309, 339, 1068, 1280, 1290, 1413, 1699
FspBI	CTAG	2 cut(s)	1104, 1976
GlaI	GCGC	3 cut(s)	83, 1264, 1974
GluI	GCNGC	8 cut(s)	71, 309, 339, 1068, 1280, 1290, 1413, 1699
GsuI	CTGGAG	2 cut(s)	1218, 1850
HaeII	RGCGCY	1 cut(s)	1976
HaeIII	GGCC	8 cut(s)	194, 350, 400, 647, 675, 1351, 1594, 2117
HapII	CCGG	4 cut(s)	605, 655, 1125, 1779
HgaI	GACGC	1 cut(s)	1007
HhaI	GCGC	3 cut(s)	84, 1265, 1975
Hin1I	GRCGYC	1 cut(s)	2025
Hin1II	CATG	9 cut(s)	1135, 1369, 1439, 1792, 1816, 1853, 1926, 2160, 2208
Hin6I	GCGC	3 cut(s)	82, 1263, 1973
HinP1I	GCGC	3 cut(s)	82, 1263, 1973
HincII	GTYRAC	3 cut(s)	451, 1765, 2023
HindII	GTYRAC	3 cut(s)	451, 1765, 2023
HinfI	GANTC	6 cut(s)	146, 319, 575, 658, 1375, 1871
HpaI	GTTAAC	1 cut(s)	1765
HpaII	CCGG	4 cut(s)	605, 655, 1125, 1779
HphI	GGTGA	3 cut(s)	504, 746, 1033
Hpy166II	GTNNAC	6 cut(s)	451, 1163, 1388, 1765, 1794, 2023
Hpy8I	GTNNAC	6 cut(s)	451, 1163, 1388, 1765, 1794, 2023
Hpy99I	CGWCG	2 cut(s)	819, 2030
HpyCH4III	ACNGT	6 cut(s)	516, 770, 1404, 1496, 1905, 2041
HpyCH4IV	ACGT	3 cut(s)	436, 814, 2025
HpyF10VI	GCNNNNNNNGC	7 cut(s)	79, 120, 347, 614, 921, 1981, 2143
HpyF3I	CTNAG	5 cut(s)	116, 387, 1442, 1667, 1914
HpySE526I	ACGT	3 cut(s)	436, 814, 2025
Hsp92I	GRCGYC	1 cut(s)	2025
Hsp92II	CATG	9 cut(s)	1135, 1369, 1439, 1792, 1816, 1853, 1926, 2160, 2208
HspAI	GCGC	3 cut(s)	82, 1263, 1973
Kpn2I	TCCGGA	2 cut(s)	654, 1124
Ksp22I	TGATCA	1 cut(s)	598
KspAI	GTTAAC	1 cut(s)	1765
KspI	CCGCGG	1 cut(s)	1996
LmnI	GCTCC	5 cut(s)	120, 287, 511, 1088, 1112
Lsp1109I	GCAGC	8 cut(s)	57, 320, 325, 1054, 1266, 1276, 1399, 1710
LweI	GCATC	9 cut(s)	325, 462, 780, 1025, 1047, 1276, 1632, 2167, 2189
MaeI	CTAG	2 cut(s)	1104, 1976
MaeII	ACGT	3 cut(s)	436, 814, 2025
MaeIII	GTNAC	2 cut(s)	701, 2072
MflI	RGATCY	2 cut(s)	802, 1670
MhlI	GDGCHC	1 cut(s)	1779
MlsI	TGGCCA	1 cut(s)	400
MluNI	TGGCCA	1 cut(s)	400
MlyI	GAGTC	2 cut(s)	140, 652
Mox20I	TGGCCA	1 cut(s)	400
Mph1103I	ATGCAT	2 cut(s)	1040, 2182
MroI	TCCGGA	2 cut(s)	654, 1124
MroXI	GAANNNNTTC	1 cut(s)	2145
MscI	TGGCCA	1 cut(s)	400
MseI	TTAA	7 cut(s)	344, 918, 1073, 1271, 1764, 1893, 2166
MslI	CAYNNNNRTG	6 cut(s)	594, 848, 1049, 1640, 1772, 2096
Msp20I	TGGCCA	1 cut(s)	400
MspA1I	CMGCKG	2 cut(s)	1412, 1995
MspI	CCGG	4 cut(s)	605, 655, 1125, 1779
MspR9I	CCNGG	6 cut(s)	424, 710, 784, 1197, 1779, 2195
Mva1269I	GAATGC	2 cut(s)	186, 216
MvaI	CCWGG	5 cut(s)	424, 710, 784, 1197, 2195
MvnI	CGCG	4 cut(s)	82, 1263, 1590, 1995
MwoI	GCNNNNNNNGC	7 cut(s)	79, 120, 347, 614, 921, 1981, 2143
NciI	CCSGG	1 cut(s)	1779
NdeI	CATATG	2 cut(s)	847, 978
NlaIII	CATG	9 cut(s)	1135, 1369, 1439, 1792, 1816, 1853, 1926, 2160, 2208
NlaIV	GGNNCC	6 cut(s)	55, 156, 421, 676, 1090, 1530
NsiI	ATGCAT	2 cut(s)	1040, 2182
NspI	RCATGY	3 cut(s)	1135, 1853, 2160
OliI	CACNNNNGTG	1 cut(s)	848
PasI	CCCWGGG	1 cut(s)	783
PceI	AGGCCT	1 cut(s)	350
PciI	ACATGT	1 cut(s)	1131
PctI	GAATGC	2 cut(s)	186, 216
PdmI	GAANNNNTTC	1 cut(s)	2145
PfeI	GAWTC	4 cut(s)	319, 575, 1375, 1871
PfoI	TCCNGGA	1 cut(s)	422
PkrI	GCNGC	8 cut(s)	72, 310, 340, 1069, 1281, 1291, 1414, 1700
PleI	GAGTC	2 cut(s)	140, 652
PpsI	GAGTC	2 cut(s)	140, 652
PpuMI	RGGWCCY	1 cut(s)	1528
PscI	ACATGT	1 cut(s)	1131
PsiI	TTATAA	1 cut(s)	938
Psp5II	RGGWCCY	1 cut(s)	1528
Psp6I	CCWGG	5 cut(s)	422, 708, 782, 1195, 2193
PspEI	GGTNACC	1 cut(s)	2072
PspGI	CCWGG	5 cut(s)	422, 708, 782, 1195, 2193
PspN4I	GGNNCC	6 cut(s)	55, 156, 421, 676, 1090, 1530
PspPI	GGNCC	5 cut(s)	54, 192, 674, 1528, 1592
PspPPI	RGGWCCY	1 cut(s)	1528
PsrI	GAACNNNNNNTAC	4 cut(s)	1319, 1351, 2049, 2081
PstI	CTGCAG	2 cut(s)	1294, 1732
PsuI	RGATCY	2 cut(s)	802, 1670
PvuII	CAGCTG	1 cut(s)	1412
RsaI	GTAC	1 cut(s)	1907
RsaNI	GTAC	1 cut(s)	1906
RseI	CAYNNNNRTG	6 cut(s)	594, 848, 1049, 1640, 1772, 2096
SacII	CCGCGG	1 cut(s)	1996
SaqAI	TTAA	7 cut(s)	344, 918, 1073, 1271, 1764, 1893, 2166
SatI	GCNGC	8 cut(s)	71, 309, 339, 1068, 1280, 1290, 1413, 1699
Sau96I	GGNCC	5 cut(s)	54, 192, 674, 1528, 1592
SchI	GAGTC	2 cut(s)	140, 652
ScrFI	CCNGG	6 cut(s)	424, 710, 784, 1197, 1779, 2195
SduI	GDGCHC	1 cut(s)	1779
SfaNI	GCATC	9 cut(s)	325, 462, 780, 1025, 1047, 1276, 1632, 2167, 2189
SfcI	CTRYAG	3 cut(s)	1290, 1728, 1876
Sfr303I	CCGCGG	1 cut(s)	1996
SgrBI	CCGCGG	1 cut(s)	1996
SinI	GGWCC	2 cut(s)	54, 1528
SmiMI	CAYNNNNRTG	6 cut(s)	594, 848, 1049, 1640, 1772, 2096
SmlI	CTYRAG	5 cut(s)	101, 278, 679, 1055, 2009
SmoI	CTYRAG	5 cut(s)	101, 278, 679, 1055, 2009
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	4 cut(s)	924, 1590, 1993, 1995
SspI	AATATT	1 cut(s)	1714
SspMI	CTAG	2 cut(s)	1104, 1976
StuI	AGGCCT	1 cut(s)	350
StyD4I	CCNGG	6 cut(s)	422, 708, 782, 1195, 1777, 2193
StyI	CCWWGG	2 cut(s)	952, 1797
TaaI	ACNGT	6 cut(s)	516, 770, 1404, 1496, 1905, 2041
TaiI	ACGT	3 cut(s)	439, 817, 2028
TaqI	TCGA	3 cut(s)	61, 1338, 2183
TfiI	GAWTC	4 cut(s)	319, 575, 1375, 1871
Tru1I	TTAA	7 cut(s)	344, 918, 1073, 1271, 1764, 1893, 2166
Tru9I	TTAA	7 cut(s)	344, 918, 1073, 1271, 1764, 1893, 2166
TscAI	CASTG	3 cut(s)	775, 873, 1501
TseI	GCWGC	8 cut(s)	70, 308, 338, 1067, 1279, 1289, 1412, 1698
TspDTI	ATGAA	3 cut(s)	209, 1452, 1566
TspGWI	ACGGA	5 cut(s)	212, 436, 896, 1037, 2179
TspRI	CASTG	3 cut(s)	775, 873, 1501
VpaK11BI	GGWCC	2 cut(s)	54, 1528
XapI	RAATTY	5 cut(s)	522, 998, 1115, 1148, 1882
XceI	RCATGY	3 cut(s)	1135, 1853, 2160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1795
XmiI	GTMKAC	2 cut(s)	1387, 1793
XmnI	GAANNNNTTC	1 cut(s)	2145
XspI	CTAG	2 cut(s)	1104, 1976
ZraI	GACGTC	1 cut(s)	2026
Zsp2I	ATGCAT	2 cut(s)	1040, 2182

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.