Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012949

Physical Location & Seq

Genomic Coordinates Reverse (-)

24603606 .. 24605442

Gene Structure 1837 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1136800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1491 bp

ATGCAAACCCATACTGGCTCAATGGAGTCTATTGTTTCGGAGCTCGAAGGAACACTTCTAAGGGATCCTGACCCCTTCTCTTACTTCATGTTGGTAGCATTTGAGGCGTCAGGTTTGATACGGTTTGCCGTGTTGTTAATGATGTGGCCGGTGATCCGGTTTCTGGAGATGATAGGTATGGAGGAAGCCGGGCTTAAACTGATGACTTTTGTTGCTGTTTTTGGTGTTCCGAAATCAGAGATTGAGTCCGTGTCTCGTGCAGTGTTGCCAAAGTTTTACATGGATGATTTGGATACGGAGGCTTGGAAAGTGTTTAGCTCATATGATAAGAGGGTTGTGGTGACTAAGATGCCAAGGATTATGGTGGAGAGGTTTGTGAAGGAGCATTTGAGAGCTGATGAAGTTATTGGCAGTGAGCTTCTTGTAAACCGATTTGGGTTTGCTACCGGCTTTGTTAAAGGTGAAGTTAACATATACAGTCGAGTGGCCAAGCTGTTTGTGGATGAGGAACAACCGACATTAGGGTTGGGAAGGCCTTCATCATCTGGTTCTTCATTTTTATCCCTATGCAAGGAACAAATCCACCCACCACTTATGATCGCCAACAAAAAGCATGATGAACAGCAAGTCCTCCGACCACTCCCGGTGATCTTCCACGACGGCCGACTAGTCAAGCGCCCTACACCTTCAACCGCCCTCTTAATCCTCCTATGGATGCCCCTAGGCATCTCACTTGCCTTCATTCGCCTATTCATGGGATTGATATTGCCAATGAGGGCTATACCCTACATGTCTCAACTATTTGGTGGTAAAGTCATTGTGAAAGGCAAACCACCACCGCCCTTCTCCGGTGGAAACTCAGGCGTGCTATTCGTCTGCACTCACCGAACCCTAATGGACCCCGTTATACTCTCCACCGTACTCAAACGTAGGATTCCAGCAGTCACATACTCTATCTCTCGACTATCAGAGCTCCTATCCCCAATCCCCACCGTTCGATTAACTCGTGTTCGGCACGTCGATGCTGAGAGAATCAAGCTTGAACTAACCAAAGGGGACTTGGTTGTTTGCCCCGAAGGAACGACGTGCCGTGAACCTTTTCTTTTGAGGTTTAGTGCACTCTTTGCTGAGCTTACAAACCGAATCGTCCCGGTGGCCATGAACTACAGGGTAGGGTTTTTCCATGCAACGACAGCAAGGGGCTGGAAAGCTTTGGACCCCATATTCTTCTTCATGAACCCTAGGCCAGTTTACGAAGTTACGTTCTTGAACCAGTTGCCTGTGGAAGCCACGTGTTCATCTGGGAAAAGCCCTCATGATGTTGCGAATTACGTGCAGAGGATCTTGGCCTCCACGTTAGGGTTTGAGTGCACCAACTTCACAAGGAAGGACAAGTACAAAGTGCTTGCTGGGAACGATGGGACCGTTTCGTATACTTCGTTTGGCGATCAGTTTAGGAAGATGGTGAGATCTTTAACCCTAAGACGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0006725 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0008374 GO:0009058 GO:0009059 GO:0009698 GO:0009699 GO:0009987 GO:0010143 GO:0010345 GO:0016020 GO:0016311 GO:0016740 GO:0016746 GO:0016747 GO:0016787 GO:0016788 GO:0016791 GO:0019438 GO:0019748 GO:0042578 GO:0043170 GO:0044237 GO:0044249 GO:0044550 GO:0071704 GO:0090447 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 map00561 map00564 map01100 map01110

Pfam Domains

Protein Families

Acyltransferase HAD

Protein Analysis

497

Amino Acids

55.89

Weight (kDa)

9.54

Isoelectric Point (pI)

44.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HAD_RAM2_N	PF23270	11 - 194	2.5e-63	Glycerol-3-phosphate acyltransferase RAM2-like, HAD-like domain
HAD	PF12710	13 - 161	2e-10	haloacid dehalogenase-like hydrolase
Acyltransferase	PF01553	286 - 387	3.8e-06	Acyltransferase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012949)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11430 AT5G06090
fragaria_vesca	FvH4_7g22870
malus_domestica	MD01G1136800.v1.1 MD07G1201800.v1.1
prunus_persica	Prupe.2G238400_v2.0.a1
pyrus_communis	pycom01g15750 pycom07g18880
rosa_chinensis	RchiOBHm_Chr1g0368021
rosa_laevigata	RLG00000027245
rosa_multiflora	Rmu_sc0011946.1_g000002
rosa_roxburghii	Rroxscaffold_4G00288930
rosa_rugosa	Rorug01G0338500
rosa_samantha	Rh1AG345300 Rh1BG307600 Rh1CG323200 Rh1DG339700
rosa_wichuraiana	Rw1G030730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1433
AciI	CCGC	2 cut(s)	693, 839
AclWI	GGATC	4 cut(s)	59, 72, 148, 1349
AcoI	YGGCCR	4 cut(s)	146, 486, 661, 1155
AcvI	CACGTG	1 cut(s)	1293
AcyI	GRCGYC	1 cut(s)	107
AfaI	GTAC	2 cut(s)	921, 1397
AfiI	CCNNNNNNNGG	6 cut(s)	163, 521, 571, 754, 848, 1359
AflIII	ACRYGT	2 cut(s)	789, 1292
AgsI	TTSAA	3 cut(s)	690, 1043, 1270
AhlI	ACTAGT	1 cut(s)	667
AjiI	CACGTC	2 cut(s)	1018, 1086
AloI	GAACNNNNNNTCC	2 cut(s)	612, 644
AluBI	AGCT	9 cut(s)	43, 318, 395, 418, 493, 973, 1039, 1132, 1211
AluI	AGCT	9 cut(s)	43, 318, 395, 418, 493, 973, 1039, 1132, 1211
Alw21I	GWGCWC	4 cut(s)	45, 975, 1120, 1373
Alw26I	GTCTC	2 cut(s)	258, 798
Alw44I	GTGCAC	2 cut(s)	1116, 1369
AlwI	GGATC	4 cut(s)	59, 72, 148, 1349
AoxI	GGCC	7 cut(s)	146, 486, 533, 661, 1155, 1244, 1347
ApaLI	GTGCAC	2 cut(s)	1116, 1369
Asp700I	GAANNNNTTC	2 cut(s)	535, 1098
AspA2I	CCTAGG	2 cut(s)	721, 1241
AspLEI	GCGC	1 cut(s)	678
AspS9I	GGNCC	3 cut(s)	898, 1216, 1422
AsuC2I	CCSGG	3 cut(s)	190, 644, 1151
AsuHPI	GGTGA	6 cut(s)	163, 352, 473, 658, 875, 1477
AvaII	GGWCC	3 cut(s)	898, 1216, 1422
AvrII	CCTAGG	2 cut(s)	721, 1241
BaeGI	GKGCMC	2 cut(s)	1120, 1373
BalI	TGGCCA	2 cut(s)	488, 1157
BamHI	GGATCC	1 cut(s)	64
BanII	GRGCYC	2 cut(s)	45, 975
BarI	GAAGNNNNNNTAC	2 cut(s)	1379, 1411
BauI	CACGAG	2 cut(s)	255, 1005
BbrPI	CACGTG	1 cut(s)	1293
Bbv12I	GWGCWC	4 cut(s)	45, 975, 1120, 1373
BccI	CCATC	2 cut(s)	1412, 1456
BceAI	ACGGC	3 cut(s)	113, 676, 1074
BcgI	CGANNNNNNTGC	2 cut(s)	1315, 1349
BciVI	GTATCC	1 cut(s)	286
BcnI	CCSGG	3 cut(s)	190, 644, 1151
BcoDI	GTCTC	2 cut(s)	258, 798
BcuI	ACTAGT	1 cut(s)	667
BfaI	CTAG	3 cut(s)	668, 722, 1242
BfmI	CTRYAG	1 cut(s)	1165
BfoI	RGCGCY	1 cut(s)	679
BfuI	GTATCC	1 cut(s)	286
BglII	AGATCT	1 cut(s)	1469
BlnI	CCTAGG	2 cut(s)	721, 1241
BlpI	GCTNAGC	1 cut(s)	1128
Bme1390I	CCNGG	3 cut(s)	190, 644, 1151
Bme18I	GGWCC	3 cut(s)	898, 1216, 1422
BmgBI	CACGTC	2 cut(s)	1018, 1086
BmgT120I	GGNCC	3 cut(s)	898, 1216, 1422
BmiI	GGNNCC	4 cut(s)	66, 900, 1218, 1423
BmrFI	CCNGG	3 cut(s)	190, 644, 1151
BmsI	GCATC	4 cut(s)	339, 705, 735, 1012
BpmI	CTGGAG	1 cut(s)	185
Bpu1102I	GCTNAGC	1 cut(s)	1128
BpuMI	CCSGG	3 cut(s)	190, 644, 1151
BsaAI	YACGTR	2 cut(s)	1293, 1333
BsaHI	GRCGYC	1 cut(s)	107
BsaJI	CCNNGG	3 cut(s)	353, 721, 1241
BsaWI	WCCGGW	2 cut(s)	156, 848
Bsc4I	CCNNNNNNNGG	6 cut(s)	163, 521, 571, 754, 848, 1359
Bse118I	RCCGGY	2 cut(s)	148, 446
Bse1I	ACTGG	3 cut(s)	19, 1247, 1273
BseDI	CCNNGG	3 cut(s)	353, 721, 1241
BseGI	GGATG	3 cut(s)	289, 508, 720
BseLI	CCNNNNNNNGG	6 cut(s)	163, 521, 571, 754, 848, 1359
BseMII	CTCAG	3 cut(s)	873, 1017, 1119
BseNI	ACTGG	3 cut(s)	19, 1247, 1273
BseSI	GKGCMC	2 cut(s)	1120, 1373
BseX3I	CGGCCG	1 cut(s)	661
BseYI	CCCAGC	1 cut(s)	1409
BsgI	GTGCAG	3 cut(s)	279, 862, 1355
Bsh1285I	CGRYCG	1 cut(s)	664
BshFI	GGCC	7 cut(s)	148, 488, 535, 663, 1157, 1246, 1349
BsiEI	CGRYCG	1 cut(s)	664
BsiHKAI	GWGCWC	4 cut(s)	45, 975, 1120, 1373
BsiSI	CCGG	7 cut(s)	149, 157, 189, 447, 644, 849, 1151
BslFI	GGGAC	3 cut(s)	1070, 1133, 1435
BslI	CCNNNNNNNGG	6 cut(s)	163, 521, 571, 754, 848, 1359
BsmAI	GTCTC	2 cut(s)	258, 798
BsmFI	GGGAC	3 cut(s)	1070, 1133, 1435
BsnI	GGCC	7 cut(s)	148, 488, 535, 663, 1157, 1246, 1349
Bsp1286I	GDGCHC	4 cut(s)	45, 975, 1120, 1373
Bsp143I	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
Bsp1720I	GCTNAGC	1 cut(s)	1128
BspACI	CCGC	2 cut(s)	693, 839
BspANI	GGCC	7 cut(s)	148, 488, 535, 663, 1157, 1246, 1349
BspCNI	CTCAG	3 cut(s)	872, 1018, 1120
BspHI	TCATGA	2 cut(s)	1233, 1315
BspLI	GGNNCC	4 cut(s)	66, 900, 1218, 1423
BspPI	GGATC	4 cut(s)	59, 72, 148, 1349
BsrFI	RCCGGY	2 cut(s)	148, 446
BsrI	ACTGG	3 cut(s)	19, 1247, 1273
BssAI	RCCGGY	2 cut(s)	148, 446
BssECI	CCNNGG	3 cut(s)	353, 721, 1241
BssMI	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
BssNAI	GTATAC	1 cut(s)	1434
BssNI	GRCGYC	1 cut(s)	107
BssSI	CACGAG	2 cut(s)	255, 1005
BssT1I	CCWWGG	3 cut(s)	353, 721, 1241
Bst1107I	GTATAC	1 cut(s)	1434
Bst2BI	CACGAG	2 cut(s)	255, 1005
Bst4CI	ACNGT	5 cut(s)	123, 479, 919, 994, 1426
BstACI	GRCGYC	1 cut(s)	107
BstAPI	GCANNNNNTGC	1 cut(s)	1124
BstBAI	YACGTR	2 cut(s)	1293, 1333
BstC8I	GCNNGC	2 cut(s)	866, 1407
BstDEI	CTNAG	6 cut(s)	59, 345, 859, 1026, 1128, 1481
BstENI	CCTNNNNNAGG	1 cut(s)	569
BstF5I	GGATG	3 cut(s)	289, 508, 720
BstH2I	RGCGCY	1 cut(s)	679
BstHHI	GCGC	1 cut(s)	678
BstKTI	GATC	7 cut(s)	67, 156, 600, 651, 1344, 1450, 1472
BstMAI	GTCTC	2 cut(s)	258, 798
BstMBI	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
BstMCI	CGRYCG	1 cut(s)	664
BstMWI	GCNNNNNNNGC	3 cut(s)	104, 1124, 1193
BstNSI	RCATGY	1 cut(s)	793
BstSCI	CCNGG	3 cut(s)	188, 642, 1149
BstSFI	CTRYAG	1 cut(s)	1165
BstSLI	GKGCMC	2 cut(s)	1120, 1373
BstX2I	RGATCY	3 cut(s)	64, 1341, 1469
BstYI	RGATCY	3 cut(s)	64, 1341, 1469
BstZ17I	GTATAC	1 cut(s)	1434
BstZI	CGGCCG	1 cut(s)	661
BsuI	GTATCC	1 cut(s)	286
BsuRI	GGCC	7 cut(s)	148, 488, 535, 663, 1157, 1246, 1349
BtrI	CACGTC	2 cut(s)	1018, 1086
BtsCI	GGATG	3 cut(s)	289, 508, 720
BtsI	GCAGTG	2 cut(s)	267, 418
BtsIMutI	CAGTG	2 cut(s)	267, 418
Cac8I	GCNNGC	2 cut(s)	866, 1407
CciI	TCATGA	2 cut(s)	1233, 1315
CfoI	GCGC	1 cut(s)	678
Cfr10I	RCCGGY	2 cut(s)	148, 446
Cfr13I	GGNCC	3 cut(s)	898, 1216, 1422
CseI	GACGC	1 cut(s)	96
Csp6I	GTAC	2 cut(s)	920, 1396
CviAII	CATG	9 cut(s)	88, 280, 614, 754, 790, 1159, 1184, 1234, 1316
CviQI	GTAC	2 cut(s)	920, 1396
DdeI	CTNAG	6 cut(s)	59, 345, 859, 1026, 1128, 1481
DpnI	GATC	7 cut(s)	66, 155, 599, 650, 1343, 1449, 1471
DpnII	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
EaeI	YGGCCR	4 cut(s)	146, 486, 661, 1155
EagI	CGGCCG	1 cut(s)	661
Ecl136II	GAGCTC	2 cut(s)	43, 973
EclXI	CGGCCG	1 cut(s)	661
Eco130I	CCWWGG	3 cut(s)	353, 721, 1241
Eco147I	AGGCCT	1 cut(s)	535
Eco24I	GRGCYC	2 cut(s)	45, 975
Eco47I	GGWCC	3 cut(s)	898, 1216, 1422
Eco52I	CGGCCG	1 cut(s)	661
Eco53kI	GAGCTC	2 cut(s)	43, 973
Eco72I	CACGTG	1 cut(s)	1293
EcoICRI	GAGCTC	2 cut(s)	43, 973
EcoNI	CCTNNNNNAGG	1 cut(s)	569
EcoT14I	CCWWGG	3 cut(s)	353, 721, 1241
EcoT38I	GRGCYC	2 cut(s)	45, 975
ErhI	CCWWGG	3 cut(s)	353, 721, 1241
FaeI	CATG	9 cut(s)	91, 283, 617, 757, 793, 1162, 1187, 1237, 1319
FalI	AAGNNNNNCTT	4 cut(s)	39, 71, 177, 209
FaqI	GGGAC	3 cut(s)	1070, 1133, 1435
FatI	CATG	9 cut(s)	87, 279, 613, 753, 789, 1158, 1183, 1233, 1315
FauNDI	CATATG	1 cut(s)	322
FblI	GTMKAC	1 cut(s)	1433
FokI	GGATG	3 cut(s)	296, 515, 727
FriOI	GRGCYC	2 cut(s)	45, 975
FspBI	CTAG	3 cut(s)	668, 722, 1242
GlaI	GCGC	1 cut(s)	677
GsaI	CCCAGC	1 cut(s)	1413
GsuI	CTGGAG	1 cut(s)	185
HaeII	RGCGCY	1 cut(s)	679
HaeIII	GGCC	7 cut(s)	148, 488, 535, 663, 1157, 1246, 1349
HapII	CCGG	7 cut(s)	149, 157, 189, 447, 644, 849, 1151
HgaI	GACGC	1 cut(s)	96
HhaI	GCGC	1 cut(s)	678
Hin1I	GRCGYC	1 cut(s)	107
Hin1II	CATG	9 cut(s)	91, 283, 617, 757, 793, 1162, 1187, 1237, 1319
Hin6I	GCGC	1 cut(s)	676
HinP1I	GCGC	1 cut(s)	676
HincII	GTYRAC	1 cut(s)	469
HindII	GTYRAC	1 cut(s)	469
HindIII	AAGCTT	2 cut(s)	1037, 1209
HinfI	GANTC	5 cut(s)	26, 245, 934, 1032, 1143
HpaI	GTTAAC	1 cut(s)	469
HpaII	CCGG	7 cut(s)	149, 157, 189, 447, 644, 849, 1151
HphI	GGTGA	6 cut(s)	163, 352, 473, 658, 875, 1477
Hpy166II	GTNNAC	7 cut(s)	427, 469, 1094, 1118, 1252, 1371, 1434
Hpy188I	TCNGA	5 cut(s)	40, 231, 238, 635, 970
Hpy188III	TCNNGA	6 cut(s)	68, 164, 960, 1234, 1267, 1316
Hpy8I	GTNNAC	7 cut(s)	427, 469, 1094, 1118, 1252, 1371, 1434
Hpy99I	CGWCG	3 cut(s)	662, 1022, 1087
HpyCH4III	ACNGT	5 cut(s)	123, 479, 919, 994, 1426
HpyCH4IV	ACGT	7 cut(s)	928, 1017, 1085, 1262, 1292, 1332, 1355
HpyCH4V	TGCA	8 cut(s)	4, 260, 570, 879, 1118, 1187, 1336, 1371
HpyF10VI	GCNNNNNNNGC	3 cut(s)	104, 1124, 1193
HpyF3I	CTNAG	6 cut(s)	59, 345, 859, 1026, 1128, 1481
HpySE526I	ACGT	7 cut(s)	928, 1017, 1085, 1262, 1292, 1332, 1355
Hsp92I	GRCGYC	1 cut(s)	107
Hsp92II	CATG	9 cut(s)	91, 283, 617, 757, 793, 1162, 1187, 1237, 1319
HspAI	GCGC	1 cut(s)	676
KspAI	GTTAAC	1 cut(s)	469
Kzo9I	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
LmnI	GCTCC	3 cut(s)	40, 382, 978
LweI	GCATC	4 cut(s)	339, 705, 735, 1012
MaeI	CTAG	3 cut(s)	668, 722, 1242
MaeII	ACGT	7 cut(s)	928, 1017, 1085, 1262, 1292, 1332, 1355
MaeIII	GTNAC	3 cut(s)	340, 943, 1258
MalI	GATC	7 cut(s)	66, 155, 599, 650, 1343, 1449, 1471
MboI	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
MboII	GAAGA	5 cut(s)	543, 643, 1219, 1222, 1471
MflI	RGATCY	3 cut(s)	64, 1341, 1469
MhlI	GDGCHC	4 cut(s)	45, 975, 1120, 1373
MlsI	TGGCCA	2 cut(s)	488, 1157
MluCI	AATT	1 cut(s)	1327
MluNI	TGGCCA	2 cut(s)	488, 1157
MlyI	GAGTC	2 cut(s)	35, 254
MmeI	TCCRAC	1 cut(s)	658
Mox20I	TGGCCA	2 cut(s)	488, 1157
MroXI	GAANNNNTTC	2 cut(s)	535, 1098
MscI	TGGCCA	2 cut(s)	488, 1157
MseI	TTAA	7 cut(s)	137, 195, 456, 468, 701, 1001, 1475
MslI	CAYNNNNRTG	1 cut(s)	1020
Msp20I	TGGCCA	2 cut(s)	488, 1157
MspI	CCGG	7 cut(s)	149, 157, 189, 447, 644, 849, 1151
MspR9I	CCNGG	3 cut(s)	190, 644, 1151
MwoI	GCNNNNNNNGC	3 cut(s)	104, 1124, 1193
NciI	CCSGG	3 cut(s)	190, 644, 1151
NdeI	CATATG	1 cut(s)	322
NdeII	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
NlaIII	CATG	9 cut(s)	91, 283, 617, 757, 793, 1162, 1187, 1237, 1319
NlaIV	GGNNCC	4 cut(s)	66, 900, 1218, 1423
NmuCI	GTSAC	2 cut(s)	340, 943
NspI	RCATGY	1 cut(s)	793
PagI	TCATGA	2 cut(s)	1233, 1315
PceI	AGGCCT	1 cut(s)	535
PciI	ACATGT	1 cut(s)	789
PcsI	WCGNNNNNNNCGW	3 cut(s)	1003, 1422, 1436
PdmI	GAANNNNTTC	2 cut(s)	535, 1098
PfeI	GAWTC	3 cut(s)	934, 1032, 1143
PleI	GAGTC	2 cut(s)	34, 253
PmaCI	CACGTG	1 cut(s)	1293
PmlI	CACGTG	1 cut(s)	1293
PpsI	GAGTC	2 cut(s)	34, 253
Ppu21I	YACGTR	2 cut(s)	1293, 1333
PscI	ACATGT	1 cut(s)	789
Psp124BI	GAGCTC	2 cut(s)	45, 975
PspCI	CACGTG	1 cut(s)	1293
PspFI	CCCAGC	1 cut(s)	1409
PspN4I	GGNNCC	4 cut(s)	66, 900, 1218, 1423
PspPI	GGNCC	3 cut(s)	898, 1216, 1422
PsuI	RGATCY	3 cut(s)	64, 1341, 1469
RsaI	GTAC	2 cut(s)	921, 1397
RsaNI	GTAC	2 cut(s)	920, 1396
RseI	CAYNNNNRTG	1 cut(s)	1020
SacI	GAGCTC	2 cut(s)	45, 975
SaqAI	TTAA	7 cut(s)	137, 195, 456, 468, 701, 1001, 1475
Sau3AI	GATC	7 cut(s)	64, 153, 597, 648, 1341, 1447, 1469
Sau96I	GGNCC	3 cut(s)	898, 1216, 1422
SchI	GAGTC	2 cut(s)	35, 254
ScrFI	CCNGG	3 cut(s)	190, 644, 1151
SduI	GDGCHC	4 cut(s)	45, 975, 1120, 1373
SfaNI	GCATC	4 cut(s)	339, 705, 735, 1012
SfcI	CTRYAG	1 cut(s)	1165
SinI	GGWCC	3 cut(s)	898, 1216, 1422
SmiMI	CAYNNNNRTG	1 cut(s)	1020
SpeI	ACTAGT	1 cut(s)	667
Sse9I	AATT	1 cut(s)	1327
SseBI	AGGCCT	1 cut(s)	535
SsiI	CCGC	2 cut(s)	693, 839
SspMI	CTAG	3 cut(s)	668, 722, 1242
SstI	GAGCTC	2 cut(s)	45, 975
StuI	AGGCCT	1 cut(s)	535
StyD4I	CCNGG	3 cut(s)	188, 642, 1149
StyI	CCWWGG	3 cut(s)	353, 721, 1241
TaaI	ACNGT	5 cut(s)	123, 479, 919, 994, 1426
TaiI	ACGT	7 cut(s)	931, 1020, 1088, 1265, 1295, 1335, 1358
TaqI	TCGA	5 cut(s)	45, 481, 961, 997, 1020
TasI	AATT	1 cut(s)	1327
TatI	WGTACW	1 cut(s)	1395
TfiI	GAWTC	3 cut(s)	934, 1032, 1143
Tru1I	TTAA	7 cut(s)	137, 195, 456, 468, 701, 1001, 1475
Tru9I	TTAA	7 cut(s)	137, 195, 456, 468, 701, 1001, 1475
TscAI	CASTG	2 cut(s)	267, 418
TseFI	GTSAC	2 cut(s)	340, 943
Tsp45I	GTSAC	2 cut(s)	340, 943
TspGWI	ACGGA	2 cut(s)	238, 311
TspRI	CASTG	2 cut(s)	267, 418
VneI	GTGCAC	2 cut(s)	1116, 1369
VpaK11BI	GGWCC	3 cut(s)	898, 1216, 1422
XagI	CCTNNNNNAGG	1 cut(s)	569
XceI	RCATGY	1 cut(s)	793
XcmI	CCANNNNNNNNNTGG	2 cut(s)	496, 1057
XmaJI	CCTAGG	2 cut(s)	721, 1241
XmiI	GTMKAC	1 cut(s)	1433
XmnI	GAANNNNTTC	2 cut(s)	535, 1098
XspI	CTAG	3 cut(s)	668, 722, 1242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.