MD02G1085600.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007575
Physical Location & Seq
Forward (+)
6718680 .. 6731559
12880 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1085600.v1.1.491

Sequence Viewer

Length: 4593 bp
ATGGCTCCACCAGTTAACATTATTGTAGGTTCTCATGTCTGGGTTGAAGATCCAAAGGAAGCATGGATTGGTGGAGAAGTTTTCCGTATTAGTGGTGAAGAAGTTCACATACGTACCAAAAATGGGAAAACGGTTGTTTCAAATATCTCAAAAGTCTTTCCCGAGGATACTGAAGCTCCTCCTGGAGGTGTGGATGACATGACAAAGCTGTCATATTTGCATGAACCAGGAGTTCTGAATAATTTGGCAGCCAGATATGAACTCAATGAAATATATACATACACTGGAAATATTTTGATTGCAGTAAATCCATTTCAAAGGTTACCACATTTGTATGACGTGCATATGATGGAACAATATAAAGGAGCCCAATTTGGGGAGTTAAGCCCTCATGTTTTTGCAATTGCAGATGTTGCATATAGAGCAATGATCAATGAGGGAAAAAGCAATTCAATTCTGGTTAGTGGAGAGAGTGGTGCTGGTAAAACAGAGACAACAAAGATGCTTATGCGATATCTTGCATACCTTGGAGGTCGAATTGGTGTAGAAGGGCGGACAGTTGAACAACAAGTTCTAGAATCCAATCCAGTTCTTGAAGCATTTGGAAATGCCAAAACTGTCAGAAACAACAACTCCAGTCGGTTTGGTAAATTCGTGGAGATTCAATTTGACAAGAATGGGAGGATCTCTGGGGCAGCTATAAGGACTTATTTGCTTGAAAGGTCTCGAGTTTGCCAAATCTCAGATCCTGAAAGAAACTATCATTGTTTTTACCTTCTTTGTGCTTCGCCGCCTGAGGAGAGAGATAAATTTAAGTTGGGAGACCCCAAACAATTCCATTACTTGAATCAATCCAGTTGCTATGAGCTGGATGGAATAGATGACGGTGAAGAATATCTTGCAACCCGAAGGGCTATGGATGTAGTTGGGATCAGTGAAGAAGAGCAGGATGCAATTTTTTTGGTAGTAGCTGCAATTCTGCATCTTGGCAATGTTGAATTTGCAAAAGGAGAGGATATTGACTCTTCTGTCATTAAGGATGAGAAGTCTAGATTCCATCTGAAGACGACAGCTGAGTTGCTTAAGTGTGATCCTAAGAGCTTGGAAGATGCTCTCATTAAGCGTGTAATGGTAACACCAGAAGAAATCATTACAAGGACTCTTGACCCTCTTTCTGCATTGGCTAGCAGAGATGCCTTAGCCAAAACTATTTATTCACGTTTGTTTGACTGGCTTGTGGAGAAAATTAACATCTCAATTGGTCAAGATCCAAACTCAAAGACATTGATTGGAGTTCTTGATATTTATGGGTTTGAGAGCTTTAAGTTCAATAGTTTTGAGCAGTTCTGTATCAATTTTACAAATGAGAAGTTGCAACAACATTTCAATCAGCATGTCTTTAAGATGGAACAGGAAGAATATACAAAAGAAGAAATCAATTGGAGCTACATTGAGTTTGTTGATAACCAAGATGTGTTGGATCTGATTGAAAAGAAACCTGGAGGGATAATTGCACTTTTAGATGAAGCCTGTATGTTTCCTAAATCCACACATGAAACATTTGCTCAGAAGTTATACCAGACATTTACAAAAAACAAGCGCTTTATCAAACCGAAGCTTTCTCGTACTAGCTTTACCATATCTCACTATGCAGGGGAGGTAACTTATCTGGCTGATCAGTTCCTTGATAAAAACAAAGATTATGTGGTTGCAGAGCACCAGGATCTGTTGACAGCCTCCAAATGCCCTTTTGTGGCAGGGCTATTTCCTCCACTTCCAGAGGATTCATCCAAGTCATCGAAGTTTTCATCCATTGGATCACGCTTCAAGTTACAACTACAATCTTTGATGGAGACCTTAAATTCAACAGAACCTCACTATATCAGATGTGTGAAGCCGAACAGTGTCCTCAAGCCTGCCATTTTTGAGAATTTTAACATTATACAACAATTACGCTGTGGTGGTGTTCTTGAGGCAATTAGGATCAGCTGCGCGGGATATCCTACCCGACGTACATTTTATGATTTTCTTCTCCGCTTTGGGGTTCTTGCTCCTGAAGTTTTGGAAGGAAACTGCGAGGACAAGGTTGCATGCCAAATGATCCTGGATAAACTGGGATTGACTGGTTATCAGATAGGCAAGACAAAGGTCTTTCTAAGAGCTGGTCAGATGGCTGAGTTGGATGCAAGGAGAGCAGAGGTACTTGGAAATGCAGCCAGAACTATACAAAGACAAATCCGTACATATGTTGCACGCAAAGAGTTCTTTGCATTACGCAAAGCTGCTATTCAATTGCAATCTTATGTGAGAGGAACATATGCTCGTGAGATATTTGAGCAGCTGCGACAACAAGCAGCAGCTGTCAAGATACAGAAAGATTTCCGACGATACATTGCCAGGAAATCATTCTCGACAGTACGGTTGTCTGCAATCACAATACAGACAGGGTTGAGAGCAATGACTGCTCGAAATGAATTCAGATTCAGAAAGCAAACTAAGGCTGCAATTCTTATCCAGACTCATTTGCGTGGCCACATAGCCTATTCTTATTATAGGAGTCTCCAGAAAGCTGCATTATATACGCAGTGTGGGTGGAGGCGTAGGGTTGCCCGGAAAGAGCTCAGAAATCTCAAAATGGCTGCAAGAGAAACAGGGGCCCTCAAAGAAGCTAAAGACAAACTAGAAAAGCGTGTGGAAGAACTTACATGGCGCCTGCAACTTGAGAAGCGATTAAGGACTGATTTAGAAGAGGAAAAGGCCCAAGAAACTGCTAAGTTGCAAGAAGCTTTGCATGCAATGCAAATACAAATAGAAGAAGCAAATGCCAGAGCAATTAGAGAACGAGAAGCACTTGAGAAAGCTGTTAAAGAAGCACCTCCTGTCATTAAGGAGACTCCTGTAATAATTCAAGACACTGAAAAAATTGACTCATTATCTGCTGAGGTGGAGAGTTTGAAGGCCTTGCTGCTCTCAGAAAGACAGGCAGCGGAAGAGGCAAGGAAAGCTTCTATTGATGCTGAGGCTAGAAATGCAGAGCTGGTCAAACAATTTGAAGATTCATGCAGAAAAGTGGACCAGCTTCAGGAATCTGCACAGAGGCTAGAGGAGAAGCTTTCGAACATAGAGTCGGAGAATCAAGTACTTCGTCAACAAGCATTGACCATGTCACCGACTGGAAAATCTCTATCTTCAAGATCAAGGACGGTGATTATTCAGAGGACACCAGAGAATGGGAATTATGTAAATGGAGAATCCAAGGTCACATCGGATATGACTCTTGCCTTATCAAATGCACGCGAACCCGAGTCAGAGGAAAAACCACAGAAATCTCTAAATGAAAAGCAGCTGGAGAACCAGGACTTGCTGGTCAAATGCATATCACAGGATTTGGGTTTTTTCGGGGGAAGACCAATTGCTGCTTGTGTCATATACAAATGTCTTCTTCACTGGAGGTCCTTTGAAGTGGAAAGGACTGGAATTTTCGACCGTGTAATTCAAACAATAGCTTCAGCCATAGAGGTCCCGGATAATAATGACGTATTAGCTTATTGGTTATCCAATACCTCAACTTTATTGTTGCTGCTCCAGCACACACTAAAGGCAAGTGGAGCAGCTAGCATGACACCACAACGGAGGAGAACATCATCAGCATCTCTTTTTGGAAGGATGTCTCAGGGGTTACGGGCTTCTCCTCAAAGTGGGGGGCTCTCATTTCTTAATGGTAGAGGACTTGGTAGACTGGATGACTTACGGCAAGTTGAGGCCAAGTATCCTGCATTATTGTTTAAGCAGCAGCTTACTGCCTTCCTTGAGAAGATATATGGAATGATGAGGGACAATTTGAAGAAGGAGATATCACCATTGCTCGGATTATGCATCCAGGCCCCCAGGACATCACGGGCAAGTTTAGTGAAAGGACGTGCCCAAGCTAATGCTGTCGCTCAGCAAGCTTTAATTGCCCATTGGCAAAGCATTGTGAAAAGCTTGGACAATTGCTTGAAGACAATGAAAGCAAATTATGTGCCTCCCTTCTTGGTCCGCAAGGTGTTCACTCAGATATTCTCATTCATCAATGTCCAGCTATTCAATAGTCTTCTTTTGCGGCGTGAGTGTTGTTCGTTCAGTAATGGGGAGTATGTGAAAGCAGGTTTGGCCGAATTAGAACAGTGGTGCTATGGGGCAAGTGAGGAATACGCCGGCTCAGCTTGGGATGAACTGAAGCATATTAGGCAGGCTGTTGGATTCCTAGTTATACATCAGAAACCGAAGAAGACCTTGAATGAAATAACAAAAGAACTGTGCCCGGTGCTTAGCATACAGCAGTTATACAGGATCAGTACAATGTACTGGGATGACAAATACGGTACACATAGTGTGTCCTCAGATGTTATTTCAAGCATGAGAGTTCTGATGACTGAGGACTCCAACAATGCTGTGAGCAGCTCTTTCCTATTAGACGATGACTCAAGCATACCATTCTCGGTGGACGACATCTCAAAGTCAATGCAACAAGTAGACGTAACTGACATTGAACCTCCACCATTGATTCGTGAACACTCTGGGTTTGGCTTCTTGCTCCCCCGGTCTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000146 GO:0000166 GO:0000331 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0005911 GO:0006928 GO:0007275 GO:0008092 GO:0008144 GO:0008150 GO:0009506 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009908 GO:0009932 GO:0009987 GO:0010015 GO:0010026 GO:0010053 GO:0010054 GO:0010090 GO:0010091 GO:0012505 GO:0015629 GO:0016043 GO:0016049 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0021700 GO:0022414 GO:0022622 GO:0030029 GO:0030048 GO:0030054 GO:0030133 GO:0030154 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0033275 GO:0035618 GO:0035619 GO:0035639 GO:0036094 GO:0040007 GO:0042623 GO:0042641 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044877 GO:0048364 GO:0048366 GO:0048367 GO:0048438 GO:0048467 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048827 GO:0048856 GO:0048869 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051645 GO:0051646 GO:0051656 GO:0055044 GO:0060151 GO:0060560 GO:0061458 GO:0070252 GO:0071695 GO:0071840 GO:0080147 GO:0090436 GO:0090558 GO:0090567 GO:0090626 GO:0090627 GO:0097159 GO:0097367 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:0140027 GO:1901265 GO:1901363 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIL IQ Myosin_N Myosin_head

Protein Analysis

1531

Amino Acids

173.23

Weight (kDa)

8.25

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_N PF02736 9 - 48 3.5e-11 Myosin N-terminal SH3-like domain
Myosin_head PF00063 64 - 720 2.6e-255 Myosin head (motor domain)
DIL PF01843 1348 - 1452 8.8e-31 DIL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 208, 1028, 3365
Acc36I ACCTGC 1 cut(s) 4136
AccB1I GGYRCC 1 cut(s) 2709
AccB7I CCANNNNNTGG 1 cut(s) 3230
AccI GTMKAC 2 cut(s) 3736, 4515
AccII CGCG 2 cut(s) 1994, 3297
AciI CCGC 7 cut(s) 553, 791, 1994, 2035, 2987, 4039, 4102
AcoI YGGCCR 2 cut(s) 2530, 4152
AcsI RAATTY 7 cut(s) 650, 809, 998, 1861, 1930, 2474, 3477
AcuI CTGAAG 6 cut(s) 192, 1082, 2076, 3065, 3492, 4238
AcyI GRCGYC 1 cut(s) 2710
AdeI CACNNNGTG 1 cut(s) 4373
AfeI AGCGCT 1 cut(s) 1601
AflII CTTAAG 1 cut(s) 1082
AjiI CACGTC 2 cut(s) 340, 3920
AjnI CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
AjuI GAANNNNNNNTTGG 4 cut(s) 51, 83, 4133, 4165
Alw21I GWGCWC 2 cut(s) 1719, 2622
Alw26I GTCTC 7 cut(s) 485, 729, 816, 1847, 2564, 2885, 3675
AlwNI CAGNNNCTG 3 cut(s) 749, 1726, 2360
Ama87I CYCGRG 3 cut(s) 161, 726, 3302
Aor51HI AGCGCT 1 cut(s) 1601
AoxI GGCC 7 cut(s) 2530, 2655, 2757, 2958, 3762, 3882, 4152
ApaI GGGCCC 1 cut(s) 2659
ApoI RAATTY 7 cut(s) 650, 809, 998, 1861, 1930, 2474, 3477
ArsI GACNNNNNNTTYG 2 cut(s) 3110, 3142
Asp700I GAANNNNTTC 3 cut(s) 102, 2378, 2405
AspLEI GCGC 3 cut(s) 1602, 1994, 2712
AspS9I GGNCC 8 cut(s) 2655, 2656, 2758, 3073, 3453, 3520, 3883, 4036
AsuC2I CCSGG 4 cut(s) 2611, 3524, 4304, 4582
AsuHPI GGTGA 5 cut(s) 107, 899, 3159, 3217, 3849
AsuII TTCGAA 1 cut(s) 3116
AsuNHI GCTAGC 2 cut(s) 1184, 3614
AvaI CYCGRG 3 cut(s) 161, 726, 3302
AvaII GGWCC 4 cut(s) 3073, 3453, 3520, 4036
AxyI CCTNAGG 1 cut(s) 795
BaeGI GKGCMC 3 cut(s) 2659, 3925, 4304
BaeI ACNNNNGTAYC 2 cut(s) 97, 130
BalI TGGCCA 1 cut(s) 2532
BanI GGYRCC 1 cut(s) 2709
BanII GRGCYC 4 cut(s) 370, 2622, 2659, 3708
BarI GAAGNNNNNNTAC 2 cut(s) 93, 125
BauI CACGAG 1 cut(s) 2322
BbsI GAAGAC 6 cut(s) 1070, 3412, 3431, 4007, 4085, 4277
Bbv12I GWGCWC 2 cut(s) 1719, 2622
BbvCI CCTCAGC 2 cut(s) 2940, 3018
BccI CCATC 6 cut(s) 343, 866, 1065, 1399, 1843, 2164
BceAI ACGGC 1 cut(s) 3767
BciT130I CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BciVI GTATCC 2 cut(s) 160, 3780
BclI TGATCA 2 cut(s) 429, 1675
BcnI CCSGG 4 cut(s) 2611, 3524, 4304, 4582
BcoDI GTCTC 7 cut(s) 485, 729, 816, 1847, 2564, 2885, 3675
BfaI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2681, 3024, 3101, 3615, 4247
BfoI RGCGCY 2 cut(s) 1603, 2713
BfrI CTTAAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 4136
BfuI GTATCC 2 cut(s) 160, 3780
BlpI GCTNAGC 3 cut(s) 3942, 4201, 4310
BmcAI AGTACT 1 cut(s) 3141
Bme18I GGWCC 4 cut(s) 3073, 3453, 3520, 4036
BmeT110I CYCGRG 3 cut(s) 161, 726, 3302
BmgBI CACGTC 2 cut(s) 340, 3920
BmgT120I GGNCC 8 cut(s) 2655, 2656, 2758, 3073, 3453, 3520, 3883, 4036
BmiI GGNNCC 7 cut(s) 6, 367, 2656, 2657, 2711, 3522, 3885
BmrI ACTGGG 2 cut(s) 2123, 4357
BmsI GCATC 9 cut(s) 492, 940, 991, 1099, 1183, 2173, 3004, 3659, 3885
BmtI GCTAGC 2 cut(s) 1188, 3618
BmuI ACTGGG 2 cut(s) 2123, 4357
BoxI GACNNNNGTC 1 cut(s) 2147
BpiI GAAGAC 6 cut(s) 1070, 3412, 3431, 4007, 4085, 4277
BpmI CTGGAG 7 cut(s) 204, 619, 1521, 2546, 3368, 3469, 3569
Bpu10I CCTNAGC 3 cut(s) 1198, 2940, 3018
Bpu1102I GCTNAGC 3 cut(s) 3942, 4201, 4310
Bpu14I TTCGAA 1 cut(s) 3116
BpuEI CTTGAG 6 cut(s) 1895, 1991, 2741, 2873, 3830, 4450
BpuMI CCSGG 4 cut(s) 2611, 3524, 4304, 4582
BsaAI YACGTR 1 cut(s) 113
BsaHI GRCGYC 1 cut(s) 2710
BsaI GGTCTC 3 cut(s) 729, 816, 1847
BsaJI CCNNGG 5 cut(s) 162, 526, 3255, 3887, 4580
BsaXI ACNNNNNCTCC 4 cut(s) 160, 190, 617, 647
Bse118I RCCGGY 1 cut(s) 4196
Bse21I CCTNAGG 1 cut(s) 795
Bse3DI GCAATG 6 cut(s) 432, 997, 2391, 2463, 2802, 3860
BseBI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BseDI CCNNGG 5 cut(s) 162, 526, 3255, 3887, 4580
BseMI GCAATG 6 cut(s) 432, 997, 2391, 2463, 2802, 3860
BseRI GAGGAG 5 cut(s) 168, 812, 3119, 3649, 3681
BseSI GKGCMC 3 cut(s) 2659, 3925, 4304
BsgI GTGCAG 1 cut(s) 3075
Bsh1236I CGCG 2 cut(s) 1994, 3297
Bsh1285I CGRYCG 1 cut(s) 3487
BshFI GGCC 7 cut(s) 2532, 2657, 2759, 2960, 3764, 3884, 4154
BshNI GGYRCC 1 cut(s) 2709
BsiEI CGRYCG 1 cut(s) 3487
BsiHKAI GWGCWC 2 cut(s) 1719, 2622
BsiHKCI CYCGRG 3 cut(s) 161, 726, 3302
BsiSI CCGG 5 cut(s) 2611, 3524, 4197, 4304, 4582
BslFI GGGAC 2 cut(s) 3506, 3848
BsmAI GTCTC 7 cut(s) 485, 729, 816, 1847, 2564, 2885, 3675
BsmFI GGGAC 2 cut(s) 3506, 3848
BsnI GGCC 7 cut(s) 2532, 2657, 2759, 2960, 3764, 3884, 4154
Bso31I GGTCTC 3 cut(s) 729, 816, 1847
BsoBI CYCGRG 3 cut(s) 161, 726, 3302
Bsp119I TTCGAA 1 cut(s) 3116
Bsp120I GGGCCC 1 cut(s) 2655
Bsp1286I GDGCHC 7 cut(s) 370, 1719, 2622, 2659, 3708, 3925, 4304
Bsp1720I GCTNAGC 3 cut(s) 3942, 4201, 4310
BspACI CCGC 7 cut(s) 553, 791, 1994, 2035, 2987, 4039, 4102
BspANI GGCC 7 cut(s) 2532, 2657, 2759, 2960, 3764, 3884, 4154
BspFNI CGCG 2 cut(s) 1994, 3297
BspLI GGNNCC 7 cut(s) 6, 367, 2656, 2657, 2711, 3522, 3885
BspMI ACCTGC 1 cut(s) 4136
BspOI GCTAGC 2 cut(s) 1188, 3618
BspQI GCTCTTC 1 cut(s) 936
BspT104I TTCGAA 1 cut(s) 3116
BspT107I GGYRCC 1 cut(s) 2709
BspTI CTTAAG 1 cut(s) 1082
BspTNI GGTCTC 3 cut(s) 729, 816, 1847
BsrDI GCAATG 6 cut(s) 432, 997, 2391, 2463, 2802, 3860
BsrFI RCCGGY 1 cut(s) 4196
BssAI RCCGGY 1 cut(s) 4196
BssECI CCNNGG 5 cut(s) 162, 526, 3255, 3887, 4580
BssNI GRCGYC 1 cut(s) 2710
BssSI CACGAG 1 cut(s) 2322
BssT1I CCWWGG 2 cut(s) 526, 3255
Bst2BI CACGAG 1 cut(s) 2322
Bst2UI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
Bst6I CTCTTC 4 cut(s) 936, 1030, 2742, 2985
BstACI GRCGYC 1 cut(s) 2710
BstAFI CTTAAG 1 cut(s) 1082
BstAPI GCANNNNNTGC 3 cut(s) 413, 2462, 2797
BstBAI YACGTR 1 cut(s) 113
BstBI TTCGAA 1 cut(s) 3116
BstEII GGTNACC 1 cut(s) 321
BstENI CCTNNNNNAGG 1 cut(s) 183
BstFNI CGCG 2 cut(s) 1994, 3297
BstH2I RGCGCY 2 cut(s) 1603, 2713
BstHHI GCGC 3 cut(s) 1602, 1994, 2712
BstMAI GTCTC 7 cut(s) 485, 729, 816, 1847, 2564, 2885, 3675
BstMCI CGRYCG 1 cut(s) 3487
BstNI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BstNSI RCATGY 3 cut(s) 1397, 2094, 2795
BstPAI GACNNNNGTC 1 cut(s) 2147
BstPI GGTNACC 1 cut(s) 321
BstSLI GKGCMC 3 cut(s) 2659, 3925, 4304
BstSNI TACGTA 1 cut(s) 113
BstUI CGCG 2 cut(s) 1994, 3297
BstV2I GAAGAC 6 cut(s) 1070, 3412, 3431, 4007, 4085, 4277
BstX2I RGATCY 6 cut(s) 49, 684, 745, 1267, 1480, 1723
BstYI RGATCY 6 cut(s) 49, 684, 745, 1267, 1480, 1723
Bsu36I CCTNAGG 1 cut(s) 795
BsuI GTATCC 2 cut(s) 160, 3780
BsuRI GGCC 7 cut(s) 2532, 2657, 2759, 2960, 3764, 3884, 4154
BtrI CACGTC 2 cut(s) 340, 3920
BtsI GCAGTG 1 cut(s) 2591
BtsIMutI CAGTG 7 cut(s) 282, 940, 1909, 2591, 2913, 3445, 4172
BveI ACCTGC 1 cut(s) 4136
CaiI CAGNNNCTG 3 cut(s) 749, 1726, 2360
CfoI GCGC 3 cut(s) 1602, 1994, 2712
Cfr10I RCCGGY 1 cut(s) 4196
Cfr13I GGNCC 8 cut(s) 2655, 2656, 2758, 3073, 3453, 3520, 3883, 4036
DinI GGCGCC 1 cut(s) 2711
DraIII CACNNNGTG 1 cut(s) 4373
DrdI GACNNNNNNGTC 3 cut(s) 208, 1028, 3365
DseDI GACNNNNNNGTC 3 cut(s) 208, 1028, 3365
EaeI YGGCCR 2 cut(s) 2530, 4152
Eam1104I CTCTTC 4 cut(s) 936, 1030, 2742, 2985
EarI CTCTTC 4 cut(s) 936, 1030, 2742, 2985
EciI GGCGGA 1 cut(s) 568
Ecl136II GAGCTC 1 cut(s) 2620
Eco105I TACGTA 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 526, 3255
Eco147I AGGCCT 1 cut(s) 2960
Eco24I GRGCYC 4 cut(s) 370, 2622, 2659, 3708
Eco31I GGTCTC 3 cut(s) 729, 816, 1847
Eco32I GATATC 3 cut(s) 515, 2000, 3855
Eco47I GGWCC 4 cut(s) 3073, 3453, 3520, 4036
Eco47III AGCGCT 1 cut(s) 1601
Eco53kI GAGCTC 1 cut(s) 2620
Eco57I CTGAAG 6 cut(s) 192, 1082, 2076, 3065, 3492, 4238
Eco81I CCTNAGG 1 cut(s) 795
Eco88I CYCGRG 3 cut(s) 161, 726, 3302
Eco91I GGTNACC 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 2620
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoO109I RGGNCCY 5 cut(s) 2655, 2656, 3453, 3520, 3883
EcoO65I GGTNACC 1 cut(s) 321
EcoRI GAATTC 1 cut(s) 2474
EcoRII CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
EcoRV GATATC 3 cut(s) 515, 2000, 3855
EcoT14I CCWWGG 2 cut(s) 526, 3255
EcoT22I ATGCAT 2 cut(s) 3377, 3878
EcoT38I GRGCYC 4 cut(s) 370, 2622, 2659, 3708
EgeI GGCGCC 1 cut(s) 2711
EheI GGCGCC 1 cut(s) 2711
ErhI CCWWGG 2 cut(s) 526, 3255
FalI AAGNNNNNCTT 6 cut(s) 882, 914, 2989, 3021, 4259, 4291
FaqI GGGAC 2 cut(s) 3506, 3848
FauI CCCGC 1 cut(s) 1987
FauNDI CATATG 3 cut(s) 345, 2245, 2317
FbaI TGATCA 2 cut(s) 429, 1675
FblI GTMKAC 2 cut(s) 3736, 4515
FriOI GRGCYC 4 cut(s) 370, 2622, 2659, 3708
FspBI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2681, 3024, 3101, 3615, 4247
GlaI GCGC 3 cut(s) 1601, 1993, 2711
GsuI CTGGAG 7 cut(s) 204, 619, 1521, 2546, 3368, 3469, 3569
HaeII RGCGCY 2 cut(s) 1603, 2713
HaeIII GGCC 7 cut(s) 2532, 2657, 2759, 2960, 3764, 3884, 4154
HapII CCGG 5 cut(s) 2611, 3524, 4197, 4304, 4582
HhaI GCGC 3 cut(s) 1602, 1994, 2712
Hin1I GRCGYC 1 cut(s) 2710
Hin6I GCGC 3 cut(s) 1600, 1992, 2710
HinP1I GCGC 3 cut(s) 1600, 1992, 2710
HincII GTYRAC 3 cut(s) 16, 1731, 3149
HindII GTYRAC 3 cut(s) 16, 1731, 3149
HindIII AAGCTT 6 cut(s) 1616, 2784, 3003, 3110, 3948, 3982
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 5 cut(s) 2611, 3524, 4197, 4304, 4582
HphI GGTGA 5 cut(s) 107, 899, 3159, 3217, 3849
Hpy99I CGWCG 2 cut(s) 2013, 2388
HpyAV CCTTC 9 cut(s) 542, 785, 903, 2060, 2950, 3657, 3814, 3841, 4039
HpyCH4IV ACGT 7 cut(s) 112, 339, 1219, 2011, 3537, 3919, 4518
HpySE526I ACGT 7 cut(s) 112, 339, 1219, 2011, 3537, 3919, 4518
Hsp92I GRCGYC 1 cut(s) 2710
HspAI GCGC 3 cut(s) 1600, 1992, 2710
KasI GGCGCC 1 cut(s) 2709
KroI GCCGGC 1 cut(s) 4196
KroNI GCCGGC 1 cut(s) 4198
Ksp22I TGATCA 2 cut(s) 429, 1675
KspAI GTTAAC 1 cut(s) 16
LguI GCTCTTC 1 cut(s) 936
LmnI GCTCC 8 cut(s) 10, 181, 365, 1443, 2056, 3588, 3608, 4581
LweI GCATC 9 cut(s) 492, 940, 991, 1099, 1183, 2173, 3004, 3659, 3885
MaeI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2681, 3024, 3101, 3615, 4247
MaeII ACGT 7 cut(s) 112, 339, 1219, 2011, 3537, 3919, 4518
MaeIII GTNAC 8 cut(s) 321, 1132, 1660, 1830, 3165, 3259, 3678, 4519
MfeI CAATTG 6 cut(s) 402, 1257, 1438, 2291, 3411, 3991
MflI RGATCY 6 cut(s) 49, 684, 745, 1267, 1480, 1723
MhlI GDGCHC 7 cut(s) 370, 1719, 2622, 2659, 3708, 3925, 4304
MlsI TGGCCA 1 cut(s) 2532
MluNI TGGCCA 1 cut(s) 2532
Mly113I GGCGCC 1 cut(s) 2710
MmeI TCCRAC 6 cut(s) 1458, 2160, 2407, 3108, 4219, 4449
Mox20I TGGCCA 1 cut(s) 2532
Mph1103I ATGCAT 2 cut(s) 3377, 3878
MroNI GCCGGC 1 cut(s) 4196
MroXI GAANNNNTTC 3 cut(s) 102, 2378, 2405
MscI TGGCCA 1 cut(s) 2532
MslI CAYNNNNRTG 2 cut(s) 333, 2526
Msp20I TGGCCA 1 cut(s) 2532
MspA1I CMGCKG 6 cut(s) 1073, 1989, 2341, 2360, 2987, 3346
MspCI CTTAAG 1 cut(s) 1082
MspI CCGG 5 cut(s) 2611, 3524, 4197, 4304, 4582
MunI CAATTG 6 cut(s) 402, 1257, 1438, 2291, 3411, 3991
MvaI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
MvnI CGCG 2 cut(s) 1994, 3297
NaeI GCCGGC 1 cut(s) 4198
NarI GGCGCC 1 cut(s) 2710
NciI CCSGG 4 cut(s) 2611, 3524, 4304, 4582
NdeI CATATG 3 cut(s) 345, 2245, 2317
NgoMIV GCCGGC 1 cut(s) 4196
NheI GCTAGC 2 cut(s) 1184, 3614
NlaIV GGNNCC 7 cut(s) 6, 367, 2656, 2657, 2711, 3522, 3885
NmuCI GTSAC 2 cut(s) 3165, 3259
NsiI ATGCAT 2 cut(s) 3377, 3878
NspI RCATGY 3 cut(s) 1397, 2094, 2795
NspV TTCGAA 1 cut(s) 3116
PaeI GCATGC 2 cut(s) 2094, 2795
PaeR7I CTCGAG 1 cut(s) 726
PceI AGGCCT 1 cut(s) 2960
PciSI GCTCTTC 1 cut(s) 936
PdiI GCCGGC 1 cut(s) 4198
PdmI GAANNNNTTC 3 cut(s) 102, 2378, 2405
PflFI GACNNNGTC 1 cut(s) 3163
PflMI CCANNNNNTGG 1 cut(s) 3230
PfoI TCCNGGA 3 cut(s) 181, 2103, 3522
PluTI GGCGCC 1 cut(s) 2713
Ppu21I YACGTR 1 cut(s) 113
PpuMI RGGWCCY 2 cut(s) 3453, 3520
PshAI GACNNNNGTC 1 cut(s) 2147
Psp124BI GAGCTC 1 cut(s) 2622
Psp5II RGGWCCY 2 cut(s) 3453, 3520
Psp6I CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
PspEI GGTNACC 1 cut(s) 321
PspGI CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
PspN4I GGNNCC 7 cut(s) 6, 367, 2656, 2657, 2711, 3522, 3885
PspOMI GGGCCC 1 cut(s) 2655
PspPI GGNCC 8 cut(s) 2655, 2656, 2758, 3073, 3453, 3520, 3883, 4036
PspPPI RGGWCCY 2 cut(s) 3453, 3520
PstNI CAGNNNCTG 3 cut(s) 749, 1726, 2360
PsuI RGATCY 6 cut(s) 49, 684, 745, 1267, 1480, 1723
PsyI GACNNNGTC 1 cut(s) 3163
PvuII CAGCTG 5 cut(s) 1073, 1989, 2341, 2360, 3346
RseI CAYNNNNRTG 2 cut(s) 333, 2526
SacI GAGCTC 1 cut(s) 2622
SapI GCTCTTC 1 cut(s) 936
Sau96I GGNCC 8 cut(s) 2655, 2656, 2758, 3073, 3453, 3520, 3883, 4036
ScaI AGTACT 1 cut(s) 3141
SduI GDGCHC 7 cut(s) 370, 1719, 2622, 2659, 3708, 3925, 4304
SfaNI GCATC 9 cut(s) 492, 940, 991, 1099, 1183, 2173, 3004, 3659, 3885
SfoI GGCGCC 1 cut(s) 2711
Sfr274I CTCGAG 1 cut(s) 726
SfuI TTCGAA 1 cut(s) 3116
SinI GGWCC 4 cut(s) 3073, 3453, 3520, 4036
SlaI CTCGAG 1 cut(s) 726
SmiMI CAYNNNNRTG 2 cut(s) 333, 2526
SmlI CTYRAG 8 cut(s) 726, 1082, 1910, 1970, 2720, 2852, 3809, 4465
SmoI CTYRAG 8 cut(s) 726, 1082, 1910, 1970, 2720, 2852, 3809, 4465
SnaBI TACGTA 1 cut(s) 113
SphI GCATGC 2 cut(s) 2094, 2795
SseBI AGGCCT 1 cut(s) 2960
SsiI CCGC 7 cut(s) 553, 791, 1994, 2035, 2987, 4039, 4102
SspDI GGCGCC 1 cut(s) 2709
SspI AATATT 1 cut(s) 292
SspMI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2681, 3024, 3101, 3615, 4247
SstI GAGCTC 1 cut(s) 2622
StuI AGGCCT 1 cut(s) 2960
StyI CCWWGG 2 cut(s) 526, 3255
TaiI ACGT 7 cut(s) 115, 342, 1222, 2014, 3540, 3922, 4521
TaqI TCGA 7 cut(s) 535, 727, 1799, 2411, 2467, 3116, 3483
TatI WGTACW 3 cut(s) 3139, 4337, 4344
TauI GCSGC 2 cut(s) 793, 4105
TscAI CASTG 7 cut(s) 289, 940, 1909, 2591, 2920, 3452, 4172
TseFI GTSAC 2 cut(s) 3165, 3259
Tsp45I GTSAC 2 cut(s) 3165, 3259
TspGWI ACGGA 3 cut(s) 74, 2228, 3646
TspRI CASTG 7 cut(s) 289, 940, 1909, 2591, 2920, 3452, 4172
Tth111I GACNNNGTC 1 cut(s) 3163
Van91I CCANNNNNTGG 1 cut(s) 3230
Vha464I CTTAAG 1 cut(s) 1082
VpaK11BI GGWCC 4 cut(s) 3073, 3453, 3520, 4036
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 7 cut(s) 650, 809, 998, 1861, 1930, 2474, 3477
XbaI TCTAGA 2 cut(s) 574, 1049
XceI RCATGY 3 cut(s) 1397, 2094, 2795
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XhoI CTCGAG 1 cut(s) 726
XmiI GTMKAC 2 cut(s) 3736, 4515
XmnI GAANNNNTTC 3 cut(s) 102, 2378, 2405
XspI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2681, 3024, 3101, 3615, 4247
ZrmI AGTACT 1 cut(s) 3141
Zsp2I ATGCAT 2 cut(s) 3377, 3878
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.