MD15G1212800.v1.1
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007575
Physical Location & Seq
Forward (+)
17083122 .. 17096171
13050 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1212800.v1.1.491

Sequence Viewer

Length: 4593 bp
ATGGCTCCACCAGTTAACATTATTGTGGGTTCTCATGTCTGGGTTGAAGACCCAAAGGAAGCATGGATTGGTGGAGAAGTTTTCCGAATTAATGGTGAAGAAGTTCACATACGTACCAAAAATGGGAAAACGGTTGTTTCAAATATCTCAAAAGTCTTTCCTGAGGATACTGAAGCTCCTCCTGGAGGTGTAGATGACATGACAAAGCTGTCATATTTGCATGAACCAGGAGTTCTGAATAATTTGGCAGCCAGATATGAACTCAATGAAATATATACATACACTGGAAATATTTTGATTGCAGTAAATCCGTTTCAAAGGTTACCACATTTGTATGACATACATATGATGGAACAATATAAAGGAGCCCAATTTGGGGAGTTAAGCCCTCATGTTTTTGCAATTGCAGATGTTGCATACAGAGCAATGATCAACGAGGGAAAAAGCAATTCAATTCTGGTTAGTGGAGAGAGTGGTGCTGGTAAAACAGAGACGACAAAGATGCTTATGCGATATCTTGCATACCTTGGAGGTCGATCTGGTGTAGAAGGGCGGACAGTTGAACAGCAAGTTCTAGAATCCAACCCAGTTCTTGAAGCATTTGGAAATGCTAAAACTGTCAGAAACAACAACTCCAGTCGGTTTGGTAAATTCGTGGAGATTCAATTTGACAAGAATGGGAGGATCTCCGGGGCAGCCATAAGGACTTATTTGCTTGAAAGGTCTCGAGTTTGCCAAATCTCAGATCCTGAAAGAAACTATCATTGCTTTTACCTTCTTTGTGCTTCACCACCTGAGGAGAGAGATAAATTTAAGTTGGGAGACCCCAAACAATTCCATTACTTGAATCAATCCAGTTGCTATGAGCTGGATGGAATAGATGACGGTGAAGAATATCTTGCAACCCGAAGGGCTATGGATATTGTTGGGATCAGTGAGGAAGAGCAGGACGCAATCTTTATGGTAGTAGCTGCAATTCTGCATCTTGGCAATGTTGAATTTGCAAAGGGAGAGGAAATTGACTCTTCTGTCATTAAGGATGAGAAGTCTAGATTCCATCTGAATACGACAGCTGAGTTGCTTAAGTGTGATCCTAAGAGTTTGGAAGATGCTCTCATTAAGCGTGTAATGGTAACACCAGAAGAAATCATCACAAGGACTCTTGACCCTGTTTCTGCATTGGCTAGCAGAGATGCCTTAGCCAAAACTGTTTATTCACGTTTGTTTGACTGGCTTGTGGAGAAAATTAACTTCTCAATTGGTCAAGATCCAAACTCAAAGGCATTGATTGGAGTTCTTGATATTTATGGGTTTGAGAGCTTTAAGTTCAATAGTTTTGAGCAGTTCTGTATCAATTTTACAAATGAGAAGTTGCAACAACATTTCAATCAGCATGTCTTTAAGATGGAACAGGAAGAATATACAAAAGAAGAAATCAATTGGAGCTACATCGAGTTTGTTGATAACCAAGATGTATTGGATCTGATTGAAAAGAAACCTGGAGGGATAATTGCACTTTTAGATGAAGCCTGTATGTTTCCTAAATCCACACATGAAACATTCGCTCAGAAGTTATACCAGACATTTACAAAAAACAAGCGCTTTATCAAACCGAAGCTTTCTCGTACTAGCTTTACCATATCTCACTATGCAGGGGAGGTAACTTATCTGGCTGATCAGTTCCTTGATAAAAACAAAGATTATGTGGTTGCAGAGCACCAGGATCTGTTGTCAGCCTCCAAATGCTCTTTTGTGGCAGGGCTATTTCCTCCACTTCCAGAGGAATCATCCAAGTCATCTAAGTTTTCATCCATTGGATCACGCTTCAAGTTACAACTACAATCTTTGATGGAGACCTTGAATTCAACAGAACCTCACTATATCAGATGTGTGAAGCCGAACAGTGTCCTCAAGCCTGCCATTTTTGAGAACTTTAACATTATACAACAATTACGCTGTGGTGGTGTTCTTGAGGCAATTAGGATCAGCTGCGCTGGATATCCTACCCGACGTACATTTTATGAGTTTCTTCTCCGCTTTGGTGTTCTTGCTCCCGAAGTTTTGGAAGGAAACTGCGAGGACAAGGTTGCATGTCAAATGATCCTGGATAAACTGGGATTGACTGGTTATCAGATAGGCAAGACAAAGGTCTTTCTAAGAGCTGGTCAGATGGCTGAGTTGGATGCAAGGAGAGCAGAGGTACTTGGAAATGCAGCTAGAACTATACAAAGGCAAATTCGTACACATGTTGCACGCAAAGAGTTCTTTGCATTACGCAAAGCTGCCATTCAATTGCAATCATATGTGAGAGGAACATATGCTCGTGAGATATTTGAGCAGCTGCGACAACAAGCAGCAGCTGTCAAGATACAGAAAGATTTCCGACGATACATTGCCAGGAAATCCTACTTGACAGTACGATTGTCTGCAATCACAATACAGACAGGGTTGAGGGCAATGACTGCTCGAAATGAATTCAGATTCAGAAAGCAAACTAAGGCTGCGATTCTTGTCCAGACTCATTTGCGTGGCCACGTAGCGTATTCTTATTATAGGAGTCTCCAGAAAGCTGCATTATATACTCAGTGTGGGTGGAGGCGTAGGGTTGCCCGGAGAGAGCTCAGAAATCTCAAAATGGCTGCAAGAGAAACGGGGGCCTTAAAAGAAGCTAAAGACAAACTAGAAAAGCGTGTGGAAGAACTTACATGGCGCTTACAACTTGAGAAGCGATTAAGGACTGATTTAGAAGAGGAAAAAACCCAAGAAACTGCTAAGTTGCAAGAAGCTTTGCATGCAATGCAAATACAAATACAAGAGGCAAATGCCAGAGCAATTAGAGAACGAGAAGAACTCGAGAAAGCTATTAAAGAAGCACCTCCTGTCATTAAGGAGACTCCTGTTATAATTCAAGACACTGAAAAAATCAACTCTTTATCAGCTGAGGTGGAGAGTTTGAAGGCCTTGTTGCTCTCGGAAAGACAGGCAGCGGAGGAGGCAAGGAAAGCTTCTATTGATGCTGAGGCTAGAAATGCAGAGCTGGTCAAACAATTTGAAGATTCATGCAGAAAAATGGATCAGCTTCAGGAATCTGTACAGAGGCTAGAGGAGAAACTTTCTAACACAGAGTCGGAAAATCAAGTACTTCGTCAACAAGCATTGACTATGTCACCGACTGGAAAGTCTCTATCGTCAAGATCAAAGACAGTGATTATTCAGAGGACATCAGAGAATGGGAATCATCTAAATGGAGAATCCAAGGTCACATCGGAAATGACTCTTGCCCTACCAAATGCACGCGAACCTGAGTCAGAGGAAAAACCACAGAAATCTCTGAATGAAAAGCAGCTGGAGAACCAGGACTTGCTGGTCAAATGCATATCACAAGATTTGGGTTTTTTCGGGGGAAGACCAATTGCTGCTTGTGTCATATACAAATGTCTTCTTCACTGGAGGTCCTTTGAAGTGGAAAGGACTGGAATTTTCGACCGTGTAATTCAAACAATAGCTTCAGCCATAGAGGTCCCGGATAATAATGACGTATTAGCTTATTGGTTATCCAATACCTCAACTTTATTATTGCTGCTCCAACACACACTTAAGGCAAGTGGAGCAGCTAGCTTGACACCACAACGGCGGAGAACGTCATCAGCATCTCTTTTTGGAAGGATGTCTCAGGGGTTACGGGCTTCTCCTCAAAGTGGGGGGCTCTCATTTATTACCGGTCGAGGACTTGGTAGACTGGATGACTTACGGCAAGTTGAGGCCAAGTATCCTGCATTATTGTTTAAGCAGCAGCTTACTGCCTTCCTTGAGAAGATATATGGAATGATAAGAGACAATTTGAAGAAGGAGATATCACCATTACTCGGATTATGTATCCAGGCCCCCAGGACATCACGGGCAAGTTTAGTGAAAGGACGTGCCCAAGCTAATGCTGTCGCTCAGCAAGCTTTAATTGCTCATTGGCAAAGCATTGTGAAAAGCTTGGACAGTTGTTTGAAGACAATGAAAGCAAATTATGTGCCTCCCTTCTTGGTGCGCAAGGTGTTCACTCAGATATTTTCATTCATCAATGTCCAGCTATTCAATAGTCTTCTTTTGCGGCGCGAGTGTTGTTCATTCAGTAATGGGGAGTATGTAAAAGCGGGTTTGGCTGAATTAGAACAGTGGTGCTACGGGGCAGGCGAGGAATACGCAGGCTCAGCTTGGGATGAACTGAAGCATATTAGGCAGGCTGTTGGATTCCTTGTTATACATCAGAAACCAAAGAAGACCTTGAATGAAATAACGAAAGAACTGTGCCCGGTGCTTAGCATACAGCAGTTATACAGGATCAGTACAATGTACTGGGATGACAAATACGGTACACACAGTGTTTCGTCAGATGTTATTTCAAGCATGAGAGTTCTAATGACCGAGGACTCCAACAATGCTGTGAGCAGCTCTTTCCTGTTAGATGACGACTCAAGCATACCATTCTCGGTGGACGACATCTCAAAGTCAATGCAACAAGTAGACATAACCGACATTGAACCTCCACCGTTGATTCGTGAAAACTCTGGGTTTGGCTTCTTACTCCCTCGGTCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000146 GO:0000166 GO:0000331 GO:0000902 GO:0000904 GO:0003006 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005856 GO:0005911 GO:0006928 GO:0007275 GO:0008092 GO:0008144 GO:0008150 GO:0009506 GO:0009653 GO:0009791 GO:0009826 GO:0009888 GO:0009908 GO:0009932 GO:0009987 GO:0010015 GO:0010026 GO:0010053 GO:0010054 GO:0010090 GO:0010091 GO:0012505 GO:0015629 GO:0016043 GO:0016049 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0021700 GO:0022414 GO:0022622 GO:0030029 GO:0030048 GO:0030054 GO:0030133 GO:0030154 GO:0030554 GO:0030898 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0032989 GO:0033275 GO:0035618 GO:0035619 GO:0035639 GO:0036094 GO:0040007 GO:0042623 GO:0042641 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043531 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044463 GO:0044464 GO:0044877 GO:0048364 GO:0048366 GO:0048367 GO:0048438 GO:0048467 GO:0048468 GO:0048469 GO:0048588 GO:0048589 GO:0048608 GO:0048731 GO:0048764 GO:0048765 GO:0048767 GO:0048768 GO:0048827 GO:0048856 GO:0048869 GO:0051015 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051645 GO:0051646 GO:0051656 GO:0055044 GO:0060151 GO:0060560 GO:0061458 GO:0070252 GO:0071695 GO:0071840 GO:0080147 GO:0090436 GO:0090558 GO:0090567 GO:0090626 GO:0090627 GO:0097159 GO:0097367 GO:0097708 GO:0099402 GO:0120025 GO:0120038 GO:0140027 GO:1901265 GO:1901363 GO:1905392
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DIL IQ Myosin_N Myosin_head

Protein Analysis

1531

Amino Acids

173.26

Weight (kDa)

8.25

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_N PF02736 9 - 48 1.9e-11 Myosin N-terminal SH3-like domain
Myosin_head PF00063 64 - 720 9.4e-256 Myosin head (motor domain)
DIL PF01843 1348 - 1452 3.9e-31 DIL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2903
AasI GACNNNNNNGTC 4 cut(s) 208, 1028, 3178, 3365
Acc16I TGCGCA 1 cut(s) 4040
AccI GTMKAC 2 cut(s) 3736, 4515
AccII CGCG 2 cut(s) 3297, 4107
AciI CCGC 6 cut(s) 553, 2035, 2987, 3634, 4102, 4145
AcoI YGGCCR 1 cut(s) 2530
AcsI RAATTY 7 cut(s) 650, 809, 998, 1861, 2235, 2474, 3477
AcuI CTGAAG 4 cut(s) 192, 3065, 3492, 4238
AdeI CACNNNGTG 1 cut(s) 4373
AfeI AGCGCT 1 cut(s) 1601
AfiI CCNNNNNNNGG 6 cut(s) 123, 185, 375, 376, 3698, 3866
AflII CTTAAG 2 cut(s) 1082, 3596
AflIII ACRYGT 1 cut(s) 2245
AgeI ACCGGT 1 cut(s) 3719
AjiI CACGTC 1 cut(s) 3920
AjnI CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
Alw21I GWGCWC 2 cut(s) 1719, 2622
Alw26I GTCTC 9 cut(s) 485, 729, 816, 1847, 2564, 2885, 3186, 3675, 3828
AlwNI CAGNNNCTG 3 cut(s) 749, 1726, 2360
Ama87I CYCGRG 2 cut(s) 726, 2852
Aor51HI AGCGCT 1 cut(s) 1601
AoxI GGCC 5 cut(s) 2530, 2655, 2958, 3762, 3882
ApoI RAATTY 7 cut(s) 650, 809, 998, 1861, 2235, 2474, 3477
AseI ATTAAT 1 cut(s) 90
AsiGI ACCGGT 1 cut(s) 3719
Asp700I GAANNNNTTC 2 cut(s) 102, 2378
AspLEI GCGC 5 cut(s) 1602, 1994, 2712, 4041, 4107
AspS9I GGNCC 4 cut(s) 2655, 3453, 3520, 3883
AsuC2I CCSGG 4 cut(s) 691, 2611, 3524, 4304
AsuHPI GGTGA 5 cut(s) 107, 780, 899, 3159, 3849
AsuNHI GCTAGC 2 cut(s) 1184, 3614
AvaI CYCGRG 2 cut(s) 726, 2852
AvaII GGWCC 2 cut(s) 3453, 3520
AxyI CCTNAGG 2 cut(s) 162, 795
BaeGI GKGCMC 2 cut(s) 3925, 4304
BaeI ACNNNNGTAYC 2 cut(s) 97, 130
BalI TGGCCA 1 cut(s) 2532
BanII GRGCYC 3 cut(s) 370, 2622, 3708
BarI GAAGNNNNNNTAC 2 cut(s) 93, 125
BauI CACGAG 1 cut(s) 2322
BbsI GAAGAC 6 cut(s) 54, 3412, 3431, 4007, 4085, 4277
Bbv12I GWGCWC 2 cut(s) 1719, 2622
BbvCI CCTCAGC 2 cut(s) 2940, 3018
BccI CCATC 6 cut(s) 343, 866, 1065, 1399, 1843, 2164
BceAI ACGGC 2 cut(s) 3647, 3767
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciT130I CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BciVI GTATCC 3 cut(s) 160, 3780, 3887
BclI TGATCA 2 cut(s) 429, 1675
BcnI CCSGG 4 cut(s) 691, 2611, 3524, 4304
BcoDI GTCTC 9 cut(s) 485, 729, 816, 1847, 2564, 2885, 3186, 3675, 3828
BfaI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2217, 2681, 3024, 3101, 3615
BfoI RGCGCY 2 cut(s) 1603, 2713
BfrI CTTAAG 2 cut(s) 1082, 3596
BfuI GTATCC 3 cut(s) 160, 3780, 3887
BlpI GCTNAGC 3 cut(s) 3942, 4201, 4310
BmcAI AGTACT 1 cut(s) 3141
Bme18I GGWCC 2 cut(s) 3453, 3520
BmeT110I CYCGRG 2 cut(s) 726, 2852
BmgBI CACGTC 1 cut(s) 3920
BmgT120I GGNCC 4 cut(s) 2655, 3453, 3520, 3883
BmiI GGNNCC 5 cut(s) 6, 367, 2656, 3522, 3885
BmrI ACTGGG 3 cut(s) 581, 2123, 4357
BmsI GCATC 7 cut(s) 492, 991, 1099, 1183, 2173, 3004, 3659
BmtI GCTAGC 2 cut(s) 1188, 3618
BmuI ACTGGG 3 cut(s) 581, 2123, 4357
BoxI GACNNNNGTC 1 cut(s) 2147
BpiI GAAGAC 6 cut(s) 54, 3412, 3431, 4007, 4085, 4277
BplI GAGNNNNNCTC 2 cut(s) 2835, 2867
BpmI CTGGAG 6 cut(s) 204, 619, 1521, 2546, 3368, 3469
Bpu10I CCTNAGC 3 cut(s) 1198, 2940, 3018
Bpu1102I GCTNAGC 3 cut(s) 3942, 4201, 4310
BpuEI CTTGAG 5 cut(s) 1895, 1991, 2741, 3830, 4450
BpuMI CCSGG 4 cut(s) 691, 2611, 3524, 4304
BsaAI YACGTR 2 cut(s) 113, 2536
BsaI GGTCTC 3 cut(s) 729, 816, 1847
BsaJI CCNNGG 6 cut(s) 526, 690, 3255, 3887, 4416, 4580
BsaWI WCCGGW 1 cut(s) 3719
BsaXI ACNNNNNCTCC 4 cut(s) 160, 190, 617, 647
Bsc4I CCNNNNNNNGG 6 cut(s) 123, 185, 375, 376, 3698, 3866
Bse118I RCCGGY 1 cut(s) 3719
Bse21I CCTNAGG 2 cut(s) 162, 795
Bse3DI GCAATG 6 cut(s) 432, 763, 997, 2391, 2463, 2802
BseBI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BseDI CCNNGG 6 cut(s) 526, 690, 3255, 3887, 4416, 4580
BseGI GGATG 9 cut(s) 877, 1045, 1787, 1808, 2188, 3672, 3748, 4216, 4357
BseLI CCNNNNNNNGG 6 cut(s) 123, 185, 375, 376, 3698, 3866
BseMI GCAATG 6 cut(s) 432, 763, 997, 2391, 2463, 2802
BseRI GAGGAG 5 cut(s) 168, 812, 3005, 3119, 3681
BseSI GKGCMC 2 cut(s) 3925, 4304
Bsh1236I CGCG 2 cut(s) 3297, 4107
Bsh1285I CGRYCG 2 cut(s) 3487, 3724
BshFI GGCC 5 cut(s) 2532, 2657, 2960, 3764, 3884
BshTI ACCGGT 1 cut(s) 3719
BsiEI CGRYCG 2 cut(s) 3487, 3724
BsiHKAI GWGCWC 2 cut(s) 1719, 2622
BsiHKCI CYCGRG 2 cut(s) 726, 2852
BsiSI CCGG 5 cut(s) 690, 2611, 3524, 3720, 4304
BslFI GGGAC 1 cut(s) 3506
BslI CCNNNNNNNGG 6 cut(s) 123, 185, 375, 376, 3698, 3866
BsmAI GTCTC 9 cut(s) 485, 729, 816, 1847, 2564, 2885, 3186, 3675, 3828
BsmBI CGTCTC 1 cut(s) 485
BsmFI GGGAC 1 cut(s) 3506
BsnI GGCC 5 cut(s) 2532, 2657, 2960, 3764, 3884
Bso31I GGTCTC 3 cut(s) 729, 816, 1847
BsoBI CYCGRG 2 cut(s) 726, 2852
Bsp1286I GDGCHC 6 cut(s) 370, 1719, 2622, 3708, 3925, 4304
Bsp1407I TGTACA 1 cut(s) 3091
Bsp1720I GCTNAGC 3 cut(s) 3942, 4201, 4310
BspACI CCGC 6 cut(s) 553, 2035, 2987, 3634, 4102, 4145
BspANI GGCC 5 cut(s) 2532, 2657, 2960, 3764, 3884
BspFNI CGCG 2 cut(s) 3297, 4107
BspLI GGNNCC 5 cut(s) 6, 367, 2656, 3522, 3885
BspOI GCTAGC 2 cut(s) 1188, 3618
BspQI GCTCTTC 1 cut(s) 936
BspTI CTTAAG 2 cut(s) 1082, 3596
BspTNI GGTCTC 3 cut(s) 729, 816, 1847
BsrDI GCAATG 6 cut(s) 432, 763, 997, 2391, 2463, 2802
BsrFI RCCGGY 1 cut(s) 3719
BsrGI TGTACA 1 cut(s) 3091
BssAI RCCGGY 1 cut(s) 3719
BssECI CCNNGG 6 cut(s) 526, 690, 3255, 3887, 4416, 4580
BssSI CACGAG 1 cut(s) 2322
BssT1I CCWWGG 2 cut(s) 526, 3255
Bst2BI CACGAG 1 cut(s) 2322
Bst2UI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
Bst6I CTCTTC 3 cut(s) 936, 1030, 2742
BstAFI CTTAAG 2 cut(s) 1082, 3596
BstAPI GCANNNNNTGC 3 cut(s) 413, 2462, 2797
BstAUI TGTACA 1 cut(s) 3091
BstBAI YACGTR 2 cut(s) 113, 2536
BstEII GGTNACC 1 cut(s) 321
BstENI CCTNNNNNAGG 1 cut(s) 183
BstF5I GGATG 9 cut(s) 877, 1045, 1787, 1808, 2188, 3672, 3748, 4216, 4357
BstFNI CGCG 2 cut(s) 3297, 4107
BstH2I RGCGCY 2 cut(s) 1603, 2713
BstHHI GCGC 5 cut(s) 1602, 1994, 2712, 4041, 4107
BstMAI GTCTC 9 cut(s) 485, 729, 816, 1847, 2564, 2885, 3186, 3675, 3828
BstMCI CGRYCG 2 cut(s) 3487, 3724
BstNI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
BstNSI RCATGY 4 cut(s) 1397, 2094, 2249, 2795
BstPAI GACNNNNGTC 1 cut(s) 2147
BstPI GGTNACC 1 cut(s) 321
BstSLI GKGCMC 2 cut(s) 3925, 4304
BstSNI TACGTA 1 cut(s) 113
BstUI CGCG 2 cut(s) 3297, 4107
BstV2I GAAGAC 6 cut(s) 54, 3412, 3431, 4007, 4085, 4277
BstX2I RGATCY 5 cut(s) 684, 745, 1267, 1480, 1723
BstYI RGATCY 5 cut(s) 684, 745, 1267, 1480, 1723
Bsu36I CCTNAGG 2 cut(s) 162, 795
BsuI GTATCC 3 cut(s) 160, 3780, 3887
BsuRI GGCC 5 cut(s) 2532, 2657, 2960, 3764, 3884
BtrI CACGTC 1 cut(s) 3920
BtsCI GGATG 9 cut(s) 877, 1045, 1787, 1808, 2188, 3672, 3748, 4216, 4357
BtsIMutI CAGTG 9 cut(s) 282, 940, 1909, 2591, 2913, 3210, 3445, 4172, 4378
CaiI CAGNNNCTG 3 cut(s) 749, 1726, 2360
CfoI GCGC 5 cut(s) 1602, 1994, 2712, 4041, 4107
Cfr10I RCCGGY 1 cut(s) 3719
Cfr13I GGNCC 4 cut(s) 2655, 3453, 3520, 3883
CseI GACGC 1 cut(s) 959
CspAI ACCGGT 1 cut(s) 3719
DraIII CACNNNGTG 1 cut(s) 4373
DrdI GACNNNNNNGTC 4 cut(s) 208, 1028, 3178, 3365
DseDI GACNNNNNNGTC 4 cut(s) 208, 1028, 3178, 3365
EaeI YGGCCR 1 cut(s) 2530
Eam1104I CTCTTC 3 cut(s) 936, 1030, 2742
EarI CTCTTC 3 cut(s) 936, 1030, 2742
EciI GGCGGA 2 cut(s) 568, 3649
Ecl136II GAGCTC 1 cut(s) 2620
Eco105I TACGTA 1 cut(s) 113
Eco130I CCWWGG 2 cut(s) 526, 3255
Eco147I AGGCCT 1 cut(s) 2960
Eco24I GRGCYC 3 cut(s) 370, 2622, 3708
Eco31I GGTCTC 3 cut(s) 729, 816, 1847
Eco32I GATATC 3 cut(s) 515, 2000, 3855
Eco47I GGWCC 2 cut(s) 3453, 3520
Eco47III AGCGCT 1 cut(s) 1601
Eco53kI GAGCTC 1 cut(s) 2620
Eco57I CTGAAG 4 cut(s) 192, 3065, 3492, 4238
Eco81I CCTNAGG 2 cut(s) 162, 795
Eco88I CYCGRG 2 cut(s) 726, 2852
Eco91I GGTNACC 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 2620
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoO109I RGGNCCY 4 cut(s) 2655, 3453, 3520, 3883
EcoO65I GGTNACC 1 cut(s) 321
EcoRI GAATTC 2 cut(s) 1861, 2474
EcoRII CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
EcoRV GATATC 3 cut(s) 515, 2000, 3855
EcoT14I CCWWGG 2 cut(s) 526, 3255
EcoT22I ATGCAT 1 cut(s) 3377
EcoT38I GRGCYC 3 cut(s) 370, 2622, 3708
ErhI CCWWGG 2 cut(s) 526, 3255
Esp3I CGTCTC 1 cut(s) 485
FalI AAGNNNNNCTT 6 cut(s) 882, 914, 2989, 3021, 4259, 4291
FaqI GGGAC 1 cut(s) 3506
FauI CCCGC 1 cut(s) 4138
FauNDI CATATG 3 cut(s) 345, 2302, 2317
FbaI TGATCA 2 cut(s) 429, 1675
FblI GTMKAC 2 cut(s) 3736, 4515
FokI GGATG 9 cut(s) 884, 1052, 1774, 1795, 2195, 3679, 3755, 4223, 4364
FriOI GRGCYC 3 cut(s) 370, 2622, 3708
FspBI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2217, 2681, 3024, 3101, 3615
FspI TGCGCA 1 cut(s) 4040
GlaI GCGC 5 cut(s) 1601, 1993, 2711, 4040, 4106
GsuI CTGGAG 6 cut(s) 204, 619, 1521, 2546, 3368, 3469
HaeII RGCGCY 2 cut(s) 1603, 2713
HaeIII GGCC 5 cut(s) 2532, 2657, 2960, 3764, 3884
HapII CCGG 5 cut(s) 690, 2611, 3524, 3720, 4304
HgaI GACGC 1 cut(s) 959
HhaI GCGC 5 cut(s) 1602, 1994, 2712, 4041, 4107
Hin6I GCGC 5 cut(s) 1600, 1992, 2710, 4039, 4105
HinP1I GCGC 5 cut(s) 1600, 1992, 2710, 4039, 4105
HincII GTYRAC 2 cut(s) 16, 3149
HindII GTYRAC 2 cut(s) 16, 3149
HindIII AAGCTT 5 cut(s) 1616, 2784, 3003, 3948, 3982
HpaI GTTAAC 1 cut(s) 16
HpaII CCGG 5 cut(s) 690, 2611, 3524, 3720, 4304
HphI GGTGA 5 cut(s) 107, 780, 899, 3159, 3849
Hpy166II GTNNAC 9 cut(s) 16, 106, 2243, 3149, 3737, 4050, 4367, 4486, 4516
Hpy8I GTNNAC 9 cut(s) 16, 106, 2243, 3149, 3737, 4050, 4367, 4486, 4516
Hpy99I CGWCG 2 cut(s) 2013, 2388
HpyAV CCTTC 9 cut(s) 542, 785, 903, 2060, 2950, 3657, 3814, 3841, 4039
HpyCH4IV ACGT 7 cut(s) 112, 1219, 2011, 2535, 3537, 3641, 3919
HpySE526I ACGT 7 cut(s) 112, 1219, 2011, 2535, 3537, 3641, 3919
HspAI GCGC 5 cut(s) 1600, 1992, 2710, 4039, 4105
Ksp22I TGATCA 2 cut(s) 429, 1675
KspAI GTTAAC 1 cut(s) 16
LguI GCTCTTC 1 cut(s) 936
LmnI GCTCC 7 cut(s) 10, 181, 365, 1443, 2056, 3588, 3608
LweI GCATC 7 cut(s) 492, 991, 1099, 1183, 2173, 3004, 3659
MaeI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2217, 2681, 3024, 3101, 3615
MaeII ACGT 7 cut(s) 112, 1219, 2011, 2535, 3537, 3641, 3919
MaeIII GTNAC 7 cut(s) 321, 1132, 1660, 1830, 3165, 3259, 3678
MfeI CAATTG 5 cut(s) 402, 1257, 1438, 2291, 3411
MflI RGATCY 5 cut(s) 684, 745, 1267, 1480, 1723
MhlI GDGCHC 6 cut(s) 370, 1719, 2622, 3708, 3925, 4304
MlsI TGGCCA 1 cut(s) 2532
MluNI TGGCCA 1 cut(s) 2532
MmeI TCCRAC 7 cut(s) 606, 2160, 2407, 3108, 3610, 4219, 4449
Mox20I TGGCCA 1 cut(s) 2532
Mph1103I ATGCAT 1 cut(s) 3377
MroXI GAANNNNTTC 2 cut(s) 102, 2378
MscI TGGCCA 1 cut(s) 2532
MslI CAYNNNNRTG 5 cut(s) 23, 333, 344, 2526, 3243
Msp20I TGGCCA 1 cut(s) 2532
MspA1I CMGCKG 7 cut(s) 1073, 1989, 2341, 2360, 2939, 2987, 3346
MspCI CTTAAG 2 cut(s) 1082, 3596
MspI CCGG 5 cut(s) 690, 2611, 3524, 3720, 4304
MunI CAATTG 5 cut(s) 402, 1257, 1438, 2291, 3411
MvaI CCWGG 9 cut(s) 183, 228, 1500, 1721, 2105, 2398, 3356, 3881, 3889
MvnI CGCG 2 cut(s) 3297, 4107
NciI CCSGG 4 cut(s) 691, 2611, 3524, 4304
NdeI CATATG 3 cut(s) 345, 2302, 2317
NheI GCTAGC 2 cut(s) 1184, 3614
NlaIV GGNNCC 5 cut(s) 6, 367, 2656, 3522, 3885
NmuCI GTSAC 2 cut(s) 3165, 3259
NsbI TGCGCA 1 cut(s) 4040
NsiI ATGCAT 1 cut(s) 3377
NspI RCATGY 4 cut(s) 1397, 2094, 2249, 2795
PaeI GCATGC 1 cut(s) 2795
PaeR7I CTCGAG 2 cut(s) 726, 2852
PceI AGGCCT 1 cut(s) 2960
PciI ACATGT 1 cut(s) 2245
PciSI GCTCTTC 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 4182
PdmI GAANNNNTTC 2 cut(s) 102, 2378
PflFI GACNNNGTC 1 cut(s) 3163
PfoI TCCNGGA 3 cut(s) 181, 2103, 3522
PinAI ACCGGT 1 cut(s) 3719
Ppu21I YACGTR 2 cut(s) 113, 2536
PpuMI RGGWCCY 2 cut(s) 3453, 3520
PscI ACATGT 1 cut(s) 2245
PshAI GACNNNNGTC 1 cut(s) 2147
PshBI ATTAAT 1 cut(s) 90
PsiI TTATAA 1 cut(s) 2903
Psp124BI GAGCTC 1 cut(s) 2622
Psp5II RGGWCCY 2 cut(s) 3453, 3520
Psp6I CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
PspEI GGTNACC 1 cut(s) 321
PspGI CCWGG 9 cut(s) 181, 226, 1498, 1719, 2103, 2396, 3354, 3879, 3887
PspN4I GGNNCC 5 cut(s) 6, 367, 2656, 3522, 3885
PspPI GGNCC 4 cut(s) 2655, 3453, 3520, 3883
PspPPI RGGWCCY 2 cut(s) 3453, 3520
PstNI CAGNNNCTG 3 cut(s) 749, 1726, 2360
PsuI RGATCY 5 cut(s) 684, 745, 1267, 1480, 1723
PsyI GACNNNGTC 1 cut(s) 3163
PvuII CAGCTG 6 cut(s) 1073, 1989, 2341, 2360, 2939, 3346
RseI CAYNNNNRTG 5 cut(s) 23, 333, 344, 2526, 3243
SacI GAGCTC 1 cut(s) 2622
SapI GCTCTTC 1 cut(s) 936
Sau96I GGNCC 4 cut(s) 2655, 3453, 3520, 3883
ScaI AGTACT 1 cut(s) 3141
SduI GDGCHC 6 cut(s) 370, 1719, 2622, 3708, 3925, 4304
SfaNI GCATC 7 cut(s) 492, 991, 1099, 1183, 2173, 3004, 3659
Sfr274I CTCGAG 2 cut(s) 726, 2852
SinI GGWCC 2 cut(s) 3453, 3520
SlaI CTCGAG 2 cut(s) 726, 2852
SmiMI CAYNNNNRTG 5 cut(s) 23, 333, 344, 2526, 3243
SmlI CTYRAG 9 cut(s) 726, 1082, 1910, 1970, 2720, 2852, 3596, 3809, 4465
SmoI CTYRAG 9 cut(s) 726, 1082, 1910, 1970, 2720, 2852, 3596, 3809, 4465
SnaBI TACGTA 1 cut(s) 113
SphI GCATGC 1 cut(s) 2795
SseBI AGGCCT 1 cut(s) 2960
SsiI CCGC 6 cut(s) 553, 2035, 2987, 3634, 4102, 4145
SspI AATATT 1 cut(s) 292
SspMI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2217, 2681, 3024, 3101, 3615
SstI GAGCTC 1 cut(s) 2622
StuI AGGCCT 1 cut(s) 2960
StyI CCWWGG 2 cut(s) 526, 3255
TaiI ACGT 7 cut(s) 115, 1222, 2014, 2538, 3540, 3644, 3922
TaqI TCGA 7 cut(s) 535, 727, 1452, 2467, 2853, 3483, 3724
TaqII GACCGA 2 cut(s) 4430, 4572
TatI WGTACW 4 cut(s) 3091, 3139, 4337, 4344
TauI GCSGC 1 cut(s) 4105
TscAI CASTG 9 cut(s) 289, 940, 1909, 2591, 2920, 3210, 3452, 4172, 4378
TseFI GTSAC 2 cut(s) 3165, 3259
Tsp45I GTSAC 2 cut(s) 3165, 3259
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 9 cut(s) 289, 940, 1909, 2591, 2920, 3210, 3452, 4172, 4378
Tth111I GACNNNGTC 1 cut(s) 3163
Vha464I CTTAAG 2 cut(s) 1082, 3596
VpaK11BI GGWCC 2 cut(s) 3453, 3520
VspI ATTAAT 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 7 cut(s) 650, 809, 998, 1861, 2235, 2474, 3477
XbaI TCTAGA 2 cut(s) 574, 1049
XceI RCATGY 4 cut(s) 1397, 2094, 2249, 2795
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XhoI CTCGAG 2 cut(s) 726, 2852
XmiI GTMKAC 2 cut(s) 3736, 4515
XmnI GAANNNNTTC 2 cut(s) 102, 2378
XspI CTAG 9 cut(s) 575, 1050, 1185, 1629, 2217, 2681, 3024, 3101, 3615
ZrmI AGTACT 1 cut(s) 3141
Zsp2I ATGCAT 1 cut(s) 3377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.