MD02G1194300.v1.1

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0024363
Physical Location & Seq
Forward (+)
18524080 .. 18524775
696 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1194300.v1.1.491

Sequence Viewer

Length: 696 bp
ATGTTTCTCGTGAAAGTTTTTTCATTCCCATTCAATACGAACAAGATTTTTCTTGCCTCCTTTTCATTTGCTTGTAGAGAAAGAATGAGGGGCCGCAAAAAGATTCAAATGGAGTTGATTGAGAGTCAACATGCACGGCATGTCTCTTTCTCCAAACGCAGATCCAGTCTCTTCAAAAACGCTAGTGAGCTTTGCACCATGTGTGCTGTTCAGGTTTTACTGGTTATCTTCTCACTTGGAAGGAAGCCCTTTTCTTTTGGCCACCCTTCCGTTCATTCGGTACTTAACAGGTTTCATCAGTTTCAGAACCCTGATGCCAGTACCAATCAAAACGAGGCGACAAAAGAACCAGTTATGCAAGGTCTTAACGACAGCTTCTCTCATTTACTTGAACAAGTGAAATTTGAGCAGAAAAGGGGAAAGAAACTGAACAGGAAAATAATGGAGCATTCGGGGCCTGATTGGTTAAAAGCACCAATGGATAGCCTCAATCTCGAGGAGTTACGCAAGGTCAAGGAAACTCTTGTCGATGTAAAAAAGAAGATAAAAAAGCAAGGGGATGAGCTTCTAGCGGAACCTCTTGCATCCTCAGCCAATAATTCTATAGAGTTGTTTCATTGTCCTGAAACTAACCTAGTTGGAGACATTTCTTCTGTTCATCCTCAAGGCTCATTAACTTTTAGGGAGGAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

232

Amino Acids

26.46

Weight (kDa)

9.39

Isoelectric Point (pI)

37.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 40 - 86 1.9e-17 SRF-type transcription factor (DNA-binding and dimerisation domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024363)

Species Orthologous Gene IDs
malus_domestica MD02G1194300.v1.1
prunus_persica Prupe.6G213000_v2.0.a1
pyrus_communis pycom02g15830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 94, 572
AclWI GGATC 1 cut(s) 156
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 1 cut(s) 401
AdeI CACNNNGTG 1 cut(s) 201
AfaI GTAC 2 cut(s) 282, 322
AgsI TTSAA 4 cut(s) 34, 107, 175, 392
AluBI AGCT 3 cut(s) 190, 375, 565
AluI AGCT 3 cut(s) 190, 375, 565
Alw26I GTCTC 3 cut(s) 148, 173, 636
AlwI GGATC 1 cut(s) 156
Ama87I CYCGRG 1 cut(s) 494
AoxI GGCC 3 cut(s) 91, 259, 455
ApoI RAATTY 1 cut(s) 401
AspS9I GGNCC 2 cut(s) 91, 455
AvaI CYCGRG 1 cut(s) 494
BalI TGGCCA 1 cut(s) 261
BauI CACGAG 1 cut(s) 8
BbvCI CCTCAGC 1 cut(s) 589
BceAI ACGGC 1 cut(s) 152
BcoDI GTCTC 3 cut(s) 148, 173, 636
BfaI CTAG 3 cut(s) 183, 569, 635
BfmI CTRYAG 1 cut(s) 603
BisI GCNGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 95
BmeT110I CYCGRG 1 cut(s) 494
BmgT120I GGNCC 2 cut(s) 91, 455
BmiI GGNNCC 3 cut(s) 92, 456, 576
BmsI GCATC 2 cut(s) 304, 593
Bpu10I CCTNAGC 1 cut(s) 589
BpuEI CTTGAG 1 cut(s) 648
BsaXI ACNNNNNCTCC 1 cut(s) 677
Bse1I ACTGG 4 cut(s) 165, 225, 318, 350
BseGI GGATG 3 cut(s) 565, 584, 658
BseMII CTCAG 1 cut(s) 603
BseNI ACTGG 4 cut(s) 165, 225, 318, 350
BseRI GAGGAG 1 cut(s) 512
BshFI GGCC 3 cut(s) 93, 261, 457
BsiHKCI CYCGRG 1 cut(s) 494
BsmAI GTCTC 3 cut(s) 148, 173, 636
BsmI GAATGC 1 cut(s) 448
BsnI GGCC 3 cut(s) 93, 261, 457
BsoBI CYCGRG 1 cut(s) 494
Bsp143I GATC 1 cut(s) 161
BspACI CCGC 2 cut(s) 94, 572
BspANI GGCC 3 cut(s) 93, 261, 457
BspCNI CTCAG 1 cut(s) 602
BspLI GGNNCC 3 cut(s) 92, 456, 576
BspPI GGATC 1 cut(s) 156
BsrI ACTGG 4 cut(s) 165, 225, 318, 350
BssMI GATC 1 cut(s) 161
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst6I CTCTTC 2 cut(s) 176, 684
BstDEI CTNAG 1 cut(s) 589
BstF5I GGATG 3 cut(s) 565, 584, 658
BstKTI GATC 1 cut(s) 164
BstMAI GTCTC 3 cut(s) 148, 173, 636
BstMBI GATC 1 cut(s) 161
BstMWI GCNNNNNNNGC 2 cut(s) 454, 590
BstNSI RCATGY 2 cut(s) 134, 143
BstSFI CTRYAG 1 cut(s) 603
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
BsuRI GGCC 3 cut(s) 93, 261, 457
BtsCI GGATG 3 cut(s) 565, 584, 658
Cfr13I GGNCC 2 cut(s) 91, 455
Csp6I GTAC 2 cut(s) 281, 321
CviAII CATG 3 cut(s) 131, 140, 199
CviQI GTAC 2 cut(s) 281, 321
DdeI CTNAG 1 cut(s) 589
DpnI GATC 1 cut(s) 163
DpnII GATC 1 cut(s) 161
DraIII CACNNNGTG 1 cut(s) 201
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 2 cut(s) 176, 684
EarI CTCTTC 2 cut(s) 176, 684
Eco88I CYCGRG 1 cut(s) 494
EcoO109I RGGNCCY 1 cut(s) 455
FaeI CATG 3 cut(s) 134, 143, 202
FaiI YATR 5 cut(s) 132, 141, 200, 356, 605
FatI CATG 3 cut(s) 130, 139, 198
Fnu4HI GCNGC 1 cut(s) 94
FokI GGATG 3 cut(s) 571, 572, 645
Fsp4HI GCNGC 1 cut(s) 94
FspBI CTAG 3 cut(s) 183, 569, 635
GluI GCNGC 1 cut(s) 94
HaeIII GGCC 3 cut(s) 93, 261, 457
Hin1II CATG 3 cut(s) 134, 143, 202
HincII GTYRAC 1 cut(s) 128
HindII GTYRAC 1 cut(s) 128
HinfI GANTC 2 cut(s) 103, 124
Hpy166II GTNNAC 1 cut(s) 128
Hpy188I TCNGA 1 cut(s) 306
Hpy188III TCNNGA 3 cut(s) 10, 494, 623
Hpy8I GTNNAC 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 234, 276
HpyCH4V TGCA 4 cut(s) 134, 195, 358, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 590
HpyF3I CTNAG 1 cut(s) 589
Hsp92II CATG 3 cut(s) 134, 143, 202
Kzo9I GATC 1 cut(s) 161
LmnI GCTCC 1 cut(s) 445
LweI GCATC 2 cut(s) 304, 593
MaeI CTAG 3 cut(s) 183, 569, 635
MaeIII GTNAC 1 cut(s) 501
MalI GATC 1 cut(s) 163
MboI GATC 1 cut(s) 161
MboII GAAGA 4 cut(s) 163, 220, 553, 642
MflI RGATCY 1 cut(s) 161
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 2 cut(s) 401, 598
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 1 cut(s) 133
MmeI TCCRAC 1 cut(s) 619
MnlI CCTC 9 cut(s) 67, 81, 328, 490, 497, 588, 598, 672, 679
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 4 cut(s) 285, 366, 467, 674
Msp20I TGGCCA 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 448
MwoI GCNNNNNNNGC 2 cut(s) 454, 590
NdeII GATC 1 cut(s) 161
NlaIII CATG 3 cut(s) 134, 143, 202
NlaIV GGNNCC 3 cut(s) 92, 456, 576
NspI RCATGY 2 cut(s) 134, 143
PaeR7I CTCGAG 1 cut(s) 494
PctI GAATGC 1 cut(s) 448
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 1 cut(s) 95
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
PspN4I GGNNCC 3 cut(s) 92, 456, 576
PspPI GGNCC 2 cut(s) 91, 455
PsuI RGATCY 1 cut(s) 161
RsaI GTAC 2 cut(s) 282, 322
RsaNI GTAC 2 cut(s) 281, 321
SaqAI TTAA 4 cut(s) 285, 366, 467, 674
SatI GCNGC 1 cut(s) 94
Sau3AI GATC 1 cut(s) 161
Sau96I GGNCC 2 cut(s) 91, 455
SchI GAGTC 1 cut(s) 133
SetI ASST 9 cut(s) 192, 216, 293, 364, 377, 513, 567, 580, 636
SfaNI GCATC 2 cut(s) 304, 593
SfcI CTRYAG 1 cut(s) 603
Sfr274I CTCGAG 1 cut(s) 494
SlaI CTCGAG 1 cut(s) 494
SmlI CTYRAG 2 cut(s) 494, 663
SmoI CTYRAG 2 cut(s) 494, 663
Sse9I AATT 2 cut(s) 401, 598
SsiI CCGC 2 cut(s) 94, 572
SspMI CTAG 3 cut(s) 183, 569, 635
TaqI TCGA 2 cut(s) 495, 528
TasI AATT 2 cut(s) 401, 598
TauI GCSGC 1 cut(s) 96
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 4 cut(s) 285, 366, 467, 674
Tru9I TTAA 4 cut(s) 285, 366, 467, 674
TspDTI ATGAA 6 cut(s) 12, 54, 263, 284, 605, 647
TspGWI ACGGA 1 cut(s) 259
XapI RAATTY 1 cut(s) 401
XceI RCATGY 2 cut(s) 134, 143
XhoI CTCGAG 1 cut(s) 494
XspI CTAG 3 cut(s) 183, 569, 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.