MD02G1309500.v1.1

Carotenoid 9,10(9',10')-cleavage dioxygenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0021172
Physical Location & Seq
Forward (+)
36524117 .. 36525331
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1309500.v1.1.491

Sequence Viewer

Length: 402 bp
ATGAGATTCAACTTGAAAACTGGTCTAGCATCCCAAAAGAAACTATCAGAAGCGGCTGTAGATTTTCCCAGGGTGAATGAGAGCTACACCGGAAGGAAGCAGCGTTATGTGTACGGAACCTTGCTGGACAGCATTGCCAAGGTTACAGGAGTAGCCAAGTTTGATCTGCATGCTGAACCAGAGTTTGGAAAAACCCAAATTGAGGTTGGAGGAAATGTCCAAGGCCTCTATGACCTCAGACCAGGCAGATTTGGTTCTGAGGCTATTTTTGTCCCTCGCACTCCTGGCGTTACTTCTGAAGAAGATGATGGCTACTTAATACTCTTTGTTCATGACGAGAACACTGGGTACTATTTTCTTTTCTCAGACTATTCGTCCTTTTCCGCTGGATATCTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RPE65

Protein Analysis

134

Amino Acids

14.85

Weight (kDa)

5.35

Isoelectric Point (pI)

26.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RPE65 PF03055 1 - 119 1.5e-23 Retinal pigment epithelial membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021172)

Species Orthologous Gene IDs
malus_domestica MD02G1309500.v1.1
rosa_chinensis RchiOBHm_Chr1g0344111
rosa_multiflora Rmu_sc0002531.1_g000016
rosa_roxburghii Rroxscaffold_3G00246000 Rroxscaffold_6G00396290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 400
AccB7I CCANNNNNTGG 1 cut(s) 185
AciI CCGC 2 cut(s) 53, 384
AcuI CTGAAG 1 cut(s) 318
AfaI GTAC 2 cut(s) 113, 350
AfiI CCNNNNNNNGG 2 cut(s) 185, 202
AgsI TTSAA 2 cut(s) 10, 16
AhdI GACNNNNNGTC 1 cut(s) 373
AjnI CCWGG 3 cut(s) 68, 241, 283
AjuI GAANNNNNNNTTGG 2 cut(s) 168, 200
AluBI AGCT 1 cut(s) 84
AluI AGCT 1 cut(s) 84
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 1 cut(s) 100
AsuHPI GGTGA 1 cut(s) 85
BbvI GCAGC 1 cut(s) 112
BccI CCATC 1 cut(s) 302
BciT130I CCWGG 3 cut(s) 70, 243, 285
BfaI CTAG 1 cut(s) 26
BfmI CTRYAG 1 cut(s) 57
BisI GCNGC 2 cut(s) 54, 101
BlsI GCNGC 2 cut(s) 55, 102
Bme1390I CCNGG 3 cut(s) 70, 243, 285
BmeRI GACNNNNNGTC 1 cut(s) 373
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 3 cut(s) 70, 243, 285
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 38
BmuI ACTGGG 1 cut(s) 354
BsaJI CCNNGG 4 cut(s) 68, 69, 138, 220
BsaWI WCCGGW 1 cut(s) 89
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 2 cut(s) 185, 202
Bse1I ACTGG 2 cut(s) 25, 349
Bse3DI GCAATG 1 cut(s) 132
BseBI CCWGG 3 cut(s) 70, 243, 285
BseDI CCNNGG 4 cut(s) 68, 69, 138, 220
BseGI GGATG 1 cut(s) 29
BseLI CCNNNNNNNGG 2 cut(s) 185, 202
BseMI GCAATG 1 cut(s) 132
BseMII CTCAG 3 cut(s) 249, 250, 378
BseNI ACTGG 2 cut(s) 25, 349
BseXI GCAGC 1 cut(s) 112
BshFI GGCC 1 cut(s) 225
BsiSI CCGG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 2 cut(s) 185, 202
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 1 cut(s) 225
Bsp143I GATC 1 cut(s) 163
BspACI CCGC 2 cut(s) 53, 384
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 3 cut(s) 249, 250, 377
BspHI TCATGA 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 132
BsrI ACTGG 2 cut(s) 25, 349
BssECI CCNNGG 4 cut(s) 68, 69, 138, 220
BssMI GATC 1 cut(s) 163
BssT1I CCWWGG 2 cut(s) 138, 220
Bst2UI CCWGG 3 cut(s) 70, 243, 285
BstC8I GCNNGC 1 cut(s) 171
BstDEI CTNAG 3 cut(s) 236, 258, 364
BstF5I GGATG 1 cut(s) 29
BstKTI GATC 1 cut(s) 166
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 285
BstNI CCWGG 3 cut(s) 70, 243, 285
BstNSI RCATGY 1 cut(s) 173
BstSCI CCNGG 3 cut(s) 68, 241, 283
BstSFI CTRYAG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 112
BsuRI GGCC 1 cut(s) 225
BtsCI GGATG 1 cut(s) 29
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 171
CciI TCATGA 1 cut(s) 331
Csp6I GTAC 2 cut(s) 112, 349
CviAII CATG 2 cut(s) 170, 332
CviJI RGCY 6 cut(s) 56, 84, 155, 225, 263, 312
CviKI_1 RGCY 6 cut(s) 56, 84, 155, 225, 263, 312
CviQI GTAC 2 cut(s) 112, 349
DdeI CTNAG 3 cut(s) 236, 258, 364
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
DriI GACNNNNNGTC 1 cut(s) 373
Eam1105I GACNNNNNGTC 1 cut(s) 373
Eco130I CCWWGG 2 cut(s) 138, 220
Eco147I AGGCCT 1 cut(s) 225
Eco32I GATATC 1 cut(s) 392
Eco57I CTGAAG 1 cut(s) 318
EcoRII CCWGG 3 cut(s) 68, 241, 283
EcoRV GATATC 1 cut(s) 392
EcoT14I CCWWGG 2 cut(s) 138, 220
ErhI CCWWGG 2 cut(s) 138, 220
FaeI CATG 2 cut(s) 173, 335
FaiI YATR 5 cut(s) 108, 171, 231, 333, 400
FaqI GGGAC 1 cut(s) 257
FatI CATG 2 cut(s) 169, 331
Fnu4HI GCNGC 2 cut(s) 54, 101
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 2 cut(s) 54, 101
FspBI CTAG 1 cut(s) 26
GluI GCNGC 2 cut(s) 54, 101
HaeIII GGCC 1 cut(s) 225
HapII CCGG 1 cut(s) 90
Hin1II CATG 2 cut(s) 173, 335
HinfI GANTC 1 cut(s) 6
HpaII CCGG 1 cut(s) 90
HphI GGTGA 1 cut(s) 85
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 5 cut(s) 49, 239, 259, 298, 367
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 87
HpyCH4V TGCA 1 cut(s) 169
HpyF10VI GCNNNNNNNGC 1 cut(s) 285
HpyF3I CTNAG 3 cut(s) 236, 258, 364
Hsp92II CATG 2 cut(s) 173, 335
Kzo9I GATC 1 cut(s) 163
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 26
MaeIII GTNAC 2 cut(s) 142, 289
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 2 cut(s) 311, 314
MluCI AATT 1 cut(s) 198
MmeI TCCRAC 1 cut(s) 187
MnlI CCTC 6 cut(s) 196, 203, 236, 245, 253, 285
MseI TTAA 1 cut(s) 317
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 1 cut(s) 90
MspR9I CCNGG 3 cut(s) 70, 243, 285
MvaI CCWGG 3 cut(s) 70, 243, 285
MwoI GCNNNNNNNGC 1 cut(s) 285
NdeII GATC 1 cut(s) 163
NlaIII CATG 2 cut(s) 173, 335
NlaIV GGNNCC 1 cut(s) 118
NspI RCATGY 1 cut(s) 173
PaeI GCATGC 1 cut(s) 173
PagI TCATGA 1 cut(s) 331
PasI CCCWGGG 1 cut(s) 69
PceI AGGCCT 1 cut(s) 225
PfeI GAWTC 1 cut(s) 6
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 2 cut(s) 55, 102
PsiI TTATAA 1 cut(s) 400
Psp6I CCWGG 3 cut(s) 68, 241, 283
PspGI CCWGG 3 cut(s) 68, 241, 283
PspN4I GGNNCC 1 cut(s) 118
PsrI GAACNNNNNNTAC 2 cut(s) 332, 364
RsaI GTAC 2 cut(s) 113, 350
RsaNI GTAC 2 cut(s) 112, 349
SaqAI TTAA 1 cut(s) 317
SatI GCNGC 2 cut(s) 54, 101
Sau3AI GATC 1 cut(s) 163
ScrFI CCNGG 3 cut(s) 70, 243, 285
SetI ASST 5 cut(s) 86, 122, 144, 207, 237
SfaNI GCATC 1 cut(s) 38
SfcI CTRYAG 1 cut(s) 57
SphI GCATGC 1 cut(s) 173
Sse9I AATT 1 cut(s) 198
SseBI AGGCCT 1 cut(s) 225
SsiI CCGC 2 cut(s) 53, 384
SspMI CTAG 1 cut(s) 26
StuI AGGCCT 1 cut(s) 225
StyD4I CCNGG 3 cut(s) 68, 241, 283
StyI CCWWGG 2 cut(s) 138, 220
TasI AATT 1 cut(s) 198
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 6
Tru1I TTAA 1 cut(s) 317
Tru9I TTAA 1 cut(s) 317
TscAI CASTG 1 cut(s) 349
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 1 cut(s) 320
TspGWI ACGGA 1 cut(s) 129
TspRI CASTG 1 cut(s) 349
Van91I CCANNNNNTGG 1 cut(s) 185
XceI RCATGY 1 cut(s) 173
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XspI CTAG 1 cut(s) 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.