MD03G1035500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0024337
Physical Location & Seq
Reverse (-)
2842100 .. 2843484
1385 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1035500.v1.1.491

Sequence Viewer

Length: 561 bp
ATGGGGAAGAGAAAAGAGCGTCGTCTTGCAGCTAAGACCAACGCTGGTCGCAGAGTCAAGCTCGATCTCTTCGCGGAACCCTCCGATGGTTGGTGCGTAGAGATGTGGGTGGCTCTGCAGAACACGATGAACATGGAGAAGATACTAAATCCAAGGACCATGCTGGGGTACCCAATTCACCATCATCTTCAGGTGGGTTCCGTCAGTTTTCTTCAAAAAGGACTTTGTAGAGAGGACATGTGTGCTCAACATTATGTTGTTCGGTGGAATTTGGATACTATATGTGCAAGACATGTTATGTTATTTTCCTCCCCCCTCGAGATTTTGTTTTCAGACTGTAGGGTGGCTGTTGTCACGTTCAGAACATTCGTTGTCAGATCATGTCGGGCCCTGCCACAGTGCAATTATGAGTCACTCCCATTCGTTATGCCCTTTTTGGCAGCCGCAAAGCATTTTGGAGAGTTCGGCTGGGATTTTTTTCTATCGGTTCGTATGTCCTGGCTTTATTTTGCATTGTACTCGCTGACATTTTTCTTCCTTGTGAACAGGTGGCTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

21.82

Weight (kDa)

9.34

Isoelectric Point (pI)

56.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024337)

Species Orthologous Gene IDs
malus_domestica MD03G1035500.v1.1
rosa_chinensis RchiOBHm_Chr5g0075561
rosa_samantha Rh5CG542200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 168
AccB1I GGYRCC 1 cut(s) 168
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 74, 444
AcsI RAATTY 1 cut(s) 268
AcuI CTGAAG 1 cut(s) 173
AfaI GTAC 2 cut(s) 170, 518
AfiI CCNNNNNNNGG 3 cut(s) 86, 90, 165
AflIII ACRYGT 2 cut(s) 237, 292
AgsI TTSAA 1 cut(s) 215
AjnI CCWGG 1 cut(s) 497
AluBI AGCT 2 cut(s) 32, 61
AluI AGCT 2 cut(s) 32, 61
Alw21I GWGCWC 1 cut(s) 247
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 387
ApaI GGGCCC 1 cut(s) 391
ApeKI GCWGC 3 cut(s) 29, 440, 553
ApoI RAATTY 1 cut(s) 268
Asp718I GGTACC 1 cut(s) 168
AspS9I GGNCC 3 cut(s) 156, 387, 388
AsuHPI GGTGA 1 cut(s) 170
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 1 cut(s) 156
BaeGI GKGCMC 1 cut(s) 391
BanI GGYRCC 1 cut(s) 168
BanII GRGCYC 1 cut(s) 391
Bbv12I GWGCWC 1 cut(s) 247
BbvI GCAGC 3 cut(s) 41, 452, 540
BccI CCATC 2 cut(s) 80, 189
BcgI CGANNNNNNTGC 2 cut(s) 501, 535
BciT130I CCWGG 1 cut(s) 499
BciVI GTATCC 1 cut(s) 268
BfmI CTRYAG 2 cut(s) 116, 337
BfuI GTATCC 1 cut(s) 268
BisI GCNGC 4 cut(s) 30, 441, 444, 554
BlsI GCNGC 4 cut(s) 31, 442, 445, 555
Bme1390I CCNGG 1 cut(s) 499
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 156, 387, 388
BmiI GGNNCC 4 cut(s) 78, 170, 199, 389
BmrFI CCNGG 1 cut(s) 499
BplI GAGNNNNNCTC 2 cut(s) 45, 77
BsaJI CCNNGG 1 cut(s) 152
Bsc4I CCNNNNNNNGG 3 cut(s) 86, 90, 165
BseBI CCWGG 1 cut(s) 499
BseDI CCNNGG 1 cut(s) 152
BseLI CCNNNNNNNGG 3 cut(s) 86, 90, 165
BseSI GKGCMC 1 cut(s) 391
BseXI GCAGC 3 cut(s) 41, 452, 540
BseYI CCCAGC 2 cut(s) 163, 468
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 389
BshNI GGYRCC 1 cut(s) 168
BsiHKAI GWGCWC 1 cut(s) 247
BsiHKCI CYCGRG 1 cut(s) 317
BslI CCNNNNNNNGG 3 cut(s) 86, 90, 165
BsnI GGCC 1 cut(s) 389
BsoBI CYCGRG 1 cut(s) 317
Bsp120I GGGCCC 1 cut(s) 387
Bsp1286I GDGCHC 2 cut(s) 247, 391
Bsp143I GATC 2 cut(s) 64, 377
BspACI CCGC 2 cut(s) 74, 444
BspANI GGCC 1 cut(s) 389
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 4 cut(s) 78, 170, 199, 389
BspMAI CTGCAG 1 cut(s) 120
BspT107I GGYRCC 1 cut(s) 168
BssECI CCNNGG 1 cut(s) 152
BssMI GATC 2 cut(s) 64, 377
BssT1I CCWWGG 1 cut(s) 152
Bst2UI CCWGG 1 cut(s) 499
Bst4CI ACNGT 2 cut(s) 338, 399
Bst6I CTCTTC 2 cut(s) 2, 74
BstDEI CTNAG 1 cut(s) 33
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 2 cut(s) 67, 380
BstMBI GATC 2 cut(s) 64, 377
BstNI CCWGG 1 cut(s) 499
BstNSI RCATGY 2 cut(s) 241, 296
BstSCI CCNGG 1 cut(s) 497
BstSFI CTRYAG 2 cut(s) 116, 337
BstSLI GKGCMC 1 cut(s) 391
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 3 cut(s) 41, 452, 540
BsuI GTATCC 1 cut(s) 268
BsuRI GGCC 1 cut(s) 389
BtsIMutI CAGTG 1 cut(s) 404
Cfr13I GGNCC 3 cut(s) 156, 387, 388
CseI GACGC 1 cut(s) 8
Csp6I GTAC 2 cut(s) 169, 517
CviAII CATG 5 cut(s) 133, 160, 238, 293, 381
CviJI RGCY 9 cut(s) 32, 61, 113, 347, 389, 443, 468, 502, 553
CviKI_1 RGCY 9 cut(s) 32, 61, 113, 347, 389, 443, 468, 502, 553
CviQI GTAC 2 cut(s) 169, 517
DdeI CTNAG 1 cut(s) 33
DpnI GATC 2 cut(s) 66, 379
DpnII GATC 2 cut(s) 64, 377
Eam1104I CTCTTC 2 cut(s) 2, 74
EarI CTCTTC 2 cut(s) 2, 74
Eco130I CCWWGG 1 cut(s) 152
Eco24I GRGCYC 1 cut(s) 391
Eco47I GGWCC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 317
EcoO109I RGGNCCY 1 cut(s) 388
EcoRII CCWGG 1 cut(s) 497
EcoT14I CCWWGG 1 cut(s) 152
EcoT38I GRGCYC 1 cut(s) 391
ErhI CCWWGG 1 cut(s) 152
FaeI CATG 5 cut(s) 136, 163, 241, 296, 384
FatI CATG 5 cut(s) 132, 159, 237, 292, 380
Fnu4HI GCNGC 4 cut(s) 30, 441, 444, 554
FriOI GRGCYC 1 cut(s) 391
Fsp4HI GCNGC 4 cut(s) 30, 441, 444, 554
GluI GCNGC 4 cut(s) 30, 441, 444, 554
GsaI CCCAGC 2 cut(s) 167, 472
HaeIII GGCC 1 cut(s) 389
HgaI GACGC 1 cut(s) 8
Hin1II CATG 5 cut(s) 136, 163, 241, 296, 384
HinfI GANTC 2 cut(s) 54, 410
HphI GGTGA 1 cut(s) 170
Hpy166II GTNNAC 1 cut(s) 544
Hpy188I TCNGA 4 cut(s) 85, 334, 362, 377
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 1 cut(s) 544
Hpy99I CGWCG 1 cut(s) 24
HpyCH4III ACNGT 2 cut(s) 338, 399
HpyCH4IV ACGT 1 cut(s) 356
HpyCH4V TGCA 6 cut(s) 29, 118, 287, 402, 512, 556
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 356
Hsp92II CATG 5 cut(s) 136, 163, 241, 296, 384
KpnI GGTACC 1 cut(s) 172
Kzo9I GATC 2 cut(s) 64, 377
LpnPI CCDG 8 cut(s) 30, 149, 176, 404, 454, 484, 511, 532
Lsp1109I GCAGC 3 cut(s) 41, 452, 540
MaeII ACGT 1 cut(s) 356
MaeIII GTNAC 2 cut(s) 352, 411
MalI GATC 2 cut(s) 66, 379
MboI GATC 2 cut(s) 64, 377
MboII GAAGA 6 cut(s) 19, 61, 151, 179, 203, 526
MhlI GDGCHC 2 cut(s) 247, 391
MluCI AATT 3 cut(s) 174, 268, 403
MlyI GAGTC 2 cut(s) 63, 419
MnlI CCTC 4 cut(s) 91, 226, 319, 326
MspR9I CCNGG 1 cut(s) 499
MvaI CCWGG 1 cut(s) 499
MvnI CGCG 1 cut(s) 74
NdeII GATC 2 cut(s) 64, 377
NlaIII CATG 5 cut(s) 136, 163, 241, 296, 384
NlaIV GGNNCC 4 cut(s) 78, 170, 199, 389
NmuCI GTSAC 2 cut(s) 352, 411
NspI RCATGY 2 cut(s) 241, 296
PaeR7I CTCGAG 1 cut(s) 317
PciI ACATGT 2 cut(s) 237, 292
PkrI GCNGC 4 cut(s) 31, 442, 445, 555
PleI GAGTC 2 cut(s) 62, 418
PpsI GAGTC 2 cut(s) 62, 418
PscI ACATGT 2 cut(s) 237, 292
Psp6I CCWGG 1 cut(s) 497
PspFI CCCAGC 2 cut(s) 163, 468
PspGI CCWGG 1 cut(s) 497
PspN4I GGNNCC 4 cut(s) 78, 170, 199, 389
PspOMI GGGCCC 1 cut(s) 387
PspPI GGNCC 3 cut(s) 156, 387, 388
PstI CTGCAG 1 cut(s) 120
RsaI GTAC 2 cut(s) 170, 518
RsaNI GTAC 2 cut(s) 169, 517
SatI GCNGC 4 cut(s) 30, 441, 444, 554
Sau3AI GATC 2 cut(s) 64, 377
Sau96I GGNCC 3 cut(s) 156, 387, 388
SchI GAGTC 2 cut(s) 63, 419
ScrFI CCNGG 1 cut(s) 499
SduI GDGCHC 2 cut(s) 247, 391
SetI ASST 5 cut(s) 34, 63, 195, 359, 551
SfcI CTRYAG 2 cut(s) 116, 337
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 1 cut(s) 156
SlaI CTCGAG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 3 cut(s) 174, 268, 403
SsiI CCGC 2 cut(s) 74, 444
StyD4I CCNGG 1 cut(s) 497
StyI CCWWGG 1 cut(s) 152
TaaI ACNGT 2 cut(s) 338, 399
TaiI ACGT 1 cut(s) 359
TaqI TCGA 2 cut(s) 63, 318
TasI AATT 3 cut(s) 174, 268, 403
TatI WGTACW 1 cut(s) 516
TauI GCSGC 1 cut(s) 446
TscAI CASTG 1 cut(s) 404
TseFI GTSAC 2 cut(s) 352, 411
TseI GCWGC 3 cut(s) 29, 440, 553
Tsp45I GTSAC 2 cut(s) 352, 411
TspDTI ATGAA 1 cut(s) 143
TspGWI ACGGA 1 cut(s) 190
TspRI CASTG 1 cut(s) 404
VpaK11BI GGWCC 1 cut(s) 156
XapI RAATTY 1 cut(s) 268
XceI RCATGY 2 cut(s) 241, 296
XhoI CTCGAG 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.