Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0014991

Physical Location & Seq

Genomic Coordinates Reverse (-)

5842203 .. 5846074

Gene Structure 3872 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1071800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGGCTTCCCGGCGAAAACCCGTCACCTGGGCTCTCTTCTTCTTCTACCTCTTGTTGGTCTCCACCCAATTCATTGCGGCGCGCAAAACCCCCAAAATCAAAGGGCCTATCAAAACGGTGGTGGTCGTCGTCATGGAAAACCGCTCCTTTGACCACATCTTTGGCTGGCTCAAGTCGACCCGACCCGACATCGACGGCCTGAACGGGAACGAATCGAACCCGATTTCCGTCTTGCTACCGGGCTCGGCTCGAGTCCGCGTCTCAAACGACGCCTTTTTCATCGACTCCGACCCGGGTCACTCGTTTCAGGCGATTCGTGAACAGATATACGGGTCAAACGAGAGCTCCGAGAACCCGGCACCGATGAACGGGTTCGCTCAGCAGGCGGATAGTATGGGCGAGGGGATGGCGAGGACAGTGATGAGCGGGTTCAAGCCGGAAGTCCTCCCCGTCTACACCGGGTTAGCAAACGAGTTCGCCGTGTTCGACCGGTGGTTCGCGTCCGTGCCGGCGTCGACTCAGCCGAACCGGTTCTACGTCCACTCGGCCACGTCACACGGTGCCATGAGCAACGTCCGCAAGGACCTCATCCACGGCTTCCCTCAGAAAACGATCTTCGACTCTCTGGACGAAAATGGCCTCGACTTCGGCATTTATTACCAGAACATCCCCGCCACGCTCTTCTTCAACAGCCTGAGGAAACTGAAGCACGTGAAGAAATTCCACAGCTACGCGCTGACGTTCAAGCGGCACGCGCGGCTCGGGAAGCTGCCCAACTATGCGGTGATCGAGCAGCGGTACTTCGACGTCAAGGAGCTTCCCGCCAACGACGACCATCCGTCGCATGACGTGGCGCGCGGGCAGAGGTTCGTGAAGGAGGTGTACGAGACGCTGAGGGCTAGCCCGCAGTGGAAAGAAATGGCATTGCTGATTACTTACGACGAGCACGGCGGGTTTTACGACCATGTGCCGACGCCGGTTTCGGGTGTGCCGAGCCCGGATGGGATCGAGGGGCCCGACCCGTATTATTTCCGGTTTGACCGGTTGGGTGTGCGGGTGCCGACCATCCTTGTCTCGCCGTGGGTCGAAAAGGGTACCGTGATCCATGAGCCAACAGGGCCGAAACCAGATTCCCAATTTGAGCATTCGTCTATCCCCGCCACCGTGAAGAGGCTATTCAATTTGAAATCGAACTTCCTGACGAAGAGAGATGCATGGGCTGGTACTTTCGAGAATTACTTTACCCTCCGCAGTACTCCTCGTGATGACTGTCCAGAAACTCTTCCAGAGGTGACGACATCACTGAGGCCAGGCGGACCCAGAGAAGATTCGAGCCTCTCAGAGTTCCAAGTCGAGCTTATCCAGCTTGCATCCCAGCTCAATGGCGATCACGTCCTCAACACCTACCCTAATATTGGCGAGACCATGACAGTGCGTGAAGCAAACATTTACGCAGAGGATGCAGTGAAGAGGTTCCTGGAAGCCGGAAGAGCTGCTCTAAAAGCCGGAGCGAACGAGTCTGCAATTGTTACGATGAGAGCTTCGCTCACCAGCCGAGTAAACGCACAAGGTCATAGCTCCTACCTAGAAACTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003993 GO:0004620 GO:0004629 GO:0006629 GO:0006644 GO:0006793 GO:0006796 GO:0008081 GO:0008150 GO:0008152 GO:0009056 GO:0009395 GO:0009405 GO:0009987 GO:0016042 GO:0016298 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0034480 GO:0035821 GO:0042578 GO:0044003 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044403 GO:0044419 GO:0046434 GO:0051701 GO:0051704 GO:0051817 GO:0052008 GO:0052043 GO:0052111 GO:0052185 GO:0052188 GO:0052368 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00562 ko00564 ko00565 ko01100 ko01110 ko02024 ko04919 map00562 map00564 map00565 map01100 map01110 map02024 map04919

Pfam Domains

Protein Families

Phosphoesterase

Protein Analysis

533

Amino Acids

59.65

Weight (kDa)

6.99

Isoelectric Point (pI)

42.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Phosphoesterase	PF04185	37 - 396	4.5e-108	Phosphoesterase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G07230
fragaria_vesca	FvH4_3g38460 FvH4_3g38460
malus_domestica	MD03G1071800.v1.1 MD11G1075900.v1.1
prunus_persica	Prupe.6G058400_v2.0.a1
pyrus_communis	pycom03g05800
rosa_chinensis	RchiOBHm_Chr5g0069151
rosa_laevigata	RLG00000036040
rosa_multiflora	Rmu_sc0000936.1_g000014
rosa_roxburghii	Rroxscaffold_1G00011640
rosa_rugosa	Rorug05G0396300
rosa_samantha	Rh5AG453200 Rh5CG494800
rosa_wichuraiana	Rw0G006040 Rw5G042320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	810
Acc65I	GGTACC	1 cut(s)	1094
AccB1I	GGYRCC	4 cut(s)	358, 560, 1057, 1094
AccBSI	CCGCTC	2 cut(s)	144, 426
AccI	GTMKAC	3 cut(s)	176, 453, 515
AccII	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
AclWI	GGATC	2 cut(s)	1013, 1096
AcoI	YGGCCR	1 cut(s)	546
AcsI	RAATTY	1 cut(s)	719
AcuI	CTGAAG	1 cut(s)	725
AcvI	CACGTG	1 cut(s)	712
AcyI	GRCGYC	4 cut(s)	270, 512, 807, 974
AdeI	CACNNNGTG	1 cut(s)	560
AfaI	GTAC	5 cut(s)	800, 884, 1096, 1225, 1255
AfiI	CCNNNNNNNGG	6 cut(s)	27, 55, 368, 983, 1170, 1415
AgeI	ACCGGT	3 cut(s)	489, 528, 1041
AgsI	TTSAA	5 cut(s)	433, 688, 745, 1180, 1186
AhdI	GACNNNNNGTC	1 cut(s)	838
AjiI	CACGTC	3 cut(s)	552, 850, 1393
AjnI	CCWGG	3 cut(s)	26, 1309, 1476
AloI	GAACNNNNNNTCC	2 cut(s)	209, 241
Alw21I	GWGCWC	2 cut(s)	347, 948
Alw26I	GTCTC	5 cut(s)	64, 265, 881, 1078, 1415
AlwI	GGATC	2 cut(s)	1013, 1096
Ama87I	CYCGRG	3 cut(s)	249, 292, 761
AoxI	GGCC	7 cut(s)	104, 196, 546, 637, 1013, 1118, 1307
ApaI	GGGCCC	1 cut(s)	1017
ApeKI	GCWGC	3 cut(s)	769, 793, 1493
ApoI	RAATTY	1 cut(s)	719
ArsI	GACNNNNNNTTYG	6 cut(s)	143, 175, 479, 511, 964, 996
AsiGI	ACCGGT	3 cut(s)	489, 528, 1041
Asp700I	GAANNNNTTC	5 cut(s)	371, 530, 719, 1281, 1472
Asp718I	GGTACC	1 cut(s)	1094
AspLEI	GCGC	6 cut(s)	82, 84, 736, 757, 856, 858
AspS9I	GGNCC	6 cut(s)	104, 583, 1013, 1014, 1118, 1316
AsuC2I	CCSGG	7 cut(s)	10, 240, 293, 294, 356, 460, 998
AsuHPI	GGTGA	4 cut(s)	16, 796, 1303, 1540
AsuNHI	GCTAGC	1 cut(s)	899
AvaI	CYCGRG	3 cut(s)	249, 292, 761
AvaII	GGWCC	2 cut(s)	583, 1316
AxyI	CCTNAGG	1 cut(s)	695
BaeGI	GKGCMC	1 cut(s)	1017
BanI	GGYRCC	4 cut(s)	358, 560, 1057, 1094
BanII	GRGCYC	5 cut(s)	34, 245, 347, 998, 1017
BarI	GAAGNNNNNNTAC	2 cut(s)	866, 898
BauI	CACGAG	1 cut(s)	1260
BbrPI	CACGTG	1 cut(s)	712
Bbv12I	GWGCWC	2 cut(s)	347, 948
BbvCI	CCTCAGC	1 cut(s)	893
BbvI	GCAGC	3 cut(s)	756, 805, 1480
BccI	CCATC	4 cut(s)	400, 843, 995, 1073
BceAI	ACGGC	5 cut(s)	211, 464, 610, 964, 1063
BciT130I	CCWGG	3 cut(s)	28, 1311, 1478
BcnI	CCSGG	7 cut(s)	10, 240, 293, 294, 356, 460, 998
BcoDI	GTCTC	5 cut(s)	64, 265, 881, 1078, 1415
BfaI	CTAG	2 cut(s)	900, 1586
BglI	GCCNNNNNGGC	1 cut(s)	1117
BisI	GCNGC	6 cut(s)	78, 749, 758, 770, 794, 1494
BlpI	GCTNAGC	1 cut(s)	378
BlsI	GCNGC	6 cut(s)	79, 750, 759, 771, 795, 1495
BmcAI	AGTACT	1 cut(s)	1255
Bme18I	GGWCC	2 cut(s)	583, 1316
BmeRI	GACNNNNNGTC	1 cut(s)	838
BmeT110I	CYCGRG	3 cut(s)	249, 292, 761
BmgBI	CACGTC	3 cut(s)	552, 850, 1393
BmgT120I	GGNCC	6 cut(s)	104, 583, 1013, 1014, 1118, 1316
BmiI	GGNNCC	8 cut(s)	360, 562, 1014, 1015, 1059, 1096, 1318, 1475
BmsI	GCATC	3 cut(s)	1201, 1379, 1450
BmtI	GCTAGC	1 cut(s)	903
BoxI	GACNNNNGTC	1 cut(s)	294
BplI	GAGNNNNNCTC	2 cut(s)	1530, 1562
Bpu10I	CCTNAGC	1 cut(s)	893
Bpu1102I	GCTNAGC	1 cut(s)	378
BpuEI	CTTGAG	1 cut(s)	155
BpuMI	CCSGG	7 cut(s)	10, 240, 293, 294, 356, 460, 998
BsaAI	YACGTR	1 cut(s)	712
BsaHI	GRCGYC	4 cut(s)	270, 512, 807, 974
BsaI	GGTCTC	2 cut(s)	64, 1415
BsaJI	CCNNGG	4 cut(s)	27, 292, 592, 1079
BsaWI	WCCGGW	4 cut(s)	489, 528, 1032, 1041
BsaXI	ACNNNNNCTCC	2 cut(s)	329, 359
Bsc4I	CCNNNNNNNGG	6 cut(s)	27, 55, 368, 983, 1170, 1415
Bse118I	RCCGGY	5 cut(s)	489, 508, 528, 976, 1041
Bse21I	CCTNAGG	1 cut(s)	695
Bse3DI	GCAATG	2 cut(s)	72, 923
BseBI	CCWGG	3 cut(s)	28, 1311, 1478
BseDI	CCNNGG	4 cut(s)	27, 292, 592, 1079
BseGI	GGATG	8 cut(s)	411, 588, 666, 835, 1006, 1065, 1370, 1465
BseLI	CCNNNNNNNGG	6 cut(s)	27, 55, 368, 983, 1170, 1415
BseMI	GCAATG	2 cut(s)	72, 923
BseMII	CTCAG	7 cut(s)	392, 533, 617, 686, 884, 1295, 1353
BsePI	GCGCGC	2 cut(s)	80, 854
BseRI	GAGGAG	1 cut(s)	1248
BseSI	GKGCMC	1 cut(s)	1017
BseXI	GCAGC	3 cut(s)	756, 805, 1480
BseYI	CCCAGC	1 cut(s)	1374
Bsh1236I	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
Bsh1285I	CGRYCG	1 cut(s)	490
BshFI	GGCC	7 cut(s)	106, 198, 548, 639, 1015, 1120, 1309
BshNI	GGYRCC	4 cut(s)	358, 560, 1057, 1094
BshTI	ACCGGT	3 cut(s)	489, 528, 1041
BsiEI	CGRYCG	1 cut(s)	490
BsiHKAI	GWGCWC	2 cut(s)	347, 948
BsiHKCI	CYCGRG	3 cut(s)	249, 292, 761
BslI	CCNNNNNNNGG	6 cut(s)	27, 55, 368, 983, 1170, 1415
BsmAI	GTCTC	5 cut(s)	64, 265, 881, 1078, 1415
BsmBI	CGTCTC	2 cut(s)	265, 881
BsmI	GAATGC	1 cut(s)	1144
BsnI	GGCC	7 cut(s)	106, 198, 548, 639, 1015, 1120, 1309
Bso31I	GGTCTC	2 cut(s)	64, 1415
BsoBI	CYCGRG	3 cut(s)	249, 292, 761
Bsp120I	GGGCCC	1 cut(s)	1013
Bsp1286I	GDGCHC	6 cut(s)	34, 245, 347, 948, 998, 1017
Bsp143I	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
Bsp1720I	GCTNAGC	1 cut(s)	378
BspANI	GGCC	7 cut(s)	106, 198, 548, 639, 1015, 1120, 1309
BspCNI	CTCAG	7 cut(s)	391, 532, 616, 687, 885, 1296, 1352
BspFNI	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
BspLI	GGNNCC	8 cut(s)	360, 562, 1014, 1015, 1059, 1096, 1318, 1475
BspOI	GCTAGC	1 cut(s)	903
BspPI	GGATC	2 cut(s)	1013, 1096
BspQI	GCTCTTC	2 cut(s)	686, 1483
BspT107I	GGYRCC	4 cut(s)	358, 560, 1057, 1094
BspTNI	GGTCTC	2 cut(s)	64, 1415
BsrBI	CCGCTC	2 cut(s)	144, 426
BsrDI	GCAATG	2 cut(s)	72, 923
BsrFI	RCCGGY	5 cut(s)	489, 508, 528, 976, 1041
BssAI	RCCGGY	5 cut(s)	489, 508, 528, 976, 1041
BssECI	CCNNGG	4 cut(s)	27, 292, 592, 1079
BssHII	GCGCGC	2 cut(s)	80, 854
BssMI	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
BssNI	GRCGYC	4 cut(s)	270, 512, 807, 974
BssSI	CACGAG	1 cut(s)	1260
Bst2BI	CACGAG	1 cut(s)	1260
Bst2UI	CCWGG	3 cut(s)	28, 1311, 1478
Bst4CI	ACNGT	7 cut(s)	118, 418, 560, 1099, 1165, 1271, 1432
Bst6I	CTCTTC	7 cut(s)	41, 686, 1163, 1199, 1287, 1463, 1483
BstACI	GRCGYC	4 cut(s)	270, 512, 807, 974
BstAPI	GCANNNNNTGC	1 cut(s)	1460
BstBAI	YACGTR	1 cut(s)	712
BstDEI	CTNAG	8 cut(s)	378, 519, 603, 695, 893, 1304, 1339, 1593
BstDSI	CCRYGG	2 cut(s)	592, 1079
BstF5I	GGATG	8 cut(s)	411, 588, 666, 835, 1006, 1065, 1370, 1465
BstFNI	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
BstHHI	GCGC	6 cut(s)	82, 84, 736, 757, 856, 858
BstKTI	GATC	5 cut(s)	615, 789, 1008, 1104, 1390
BstMAI	GTCTC	5 cut(s)	64, 265, 881, 1078, 1415
BstMBI	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
BstMCI	CGRYCG	1 cut(s)	490
BstNI	CCWGG	3 cut(s)	28, 1311, 1478
BstPAI	GACNNNNGTC	1 cut(s)	294
BstSLI	GKGCMC	1 cut(s)	1017
BstUI	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
BstV1I	GCAGC	3 cut(s)	756, 805, 1480
BstXI	CCANNNNNNTGG	2 cut(s)	161, 1382
Bsu36I	CCTNAGG	1 cut(s)	695
BsuRI	GGCC	7 cut(s)	106, 198, 548, 639, 1015, 1120, 1309
BtgI	CCRYGG	2 cut(s)	592, 1079
BtrI	CACGTC	3 cut(s)	552, 850, 1393
BtsCI	GGATG	8 cut(s)	411, 588, 666, 835, 1006, 1065, 1370, 1465
BtsI	GCAGTG	2 cut(s)	914, 1470
BtsIMutI	CAGTG	5 cut(s)	423, 914, 1301, 1437, 1470
CfoI	GCGC	6 cut(s)	82, 84, 736, 757, 856, 858
Cfr10I	RCCGGY	5 cut(s)	489, 508, 528, 976, 1041
Cfr13I	GGNCC	6 cut(s)	104, 583, 1013, 1014, 1118, 1316
Cfr9I	CCCGGG	1 cut(s)	292
CseI	GACGC	6 cut(s)	247, 278, 489, 501, 898, 982
Csp6I	GTAC	5 cut(s)	799, 883, 1095, 1224, 1254
CspAI	ACCGGT	3 cut(s)	489, 528, 1041
CviAII	CATG	7 cut(s)	133, 565, 845, 965, 1106, 1215, 1426
CviQI	GTAC	5 cut(s)	799, 883, 1095, 1224, 1254
DdeI	CTNAG	8 cut(s)	378, 519, 603, 695, 893, 1304, 1339, 1593
DpnI	GATC	5 cut(s)	614, 788, 1007, 1103, 1389
DpnII	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
DraIII	CACNNNGTG	1 cut(s)	560
DriI	GACNNNNNGTC	1 cut(s)	838
EaeI	YGGCCR	1 cut(s)	546
Eam1104I	CTCTTC	7 cut(s)	41, 686, 1163, 1199, 1287, 1463, 1483
Eam1105I	GACNNNNNGTC	1 cut(s)	838
EarI	CTCTTC	7 cut(s)	41, 686, 1163, 1199, 1287, 1463, 1483
EciI	GGCGGA	2 cut(s)	401, 1329
Ecl136II	GAGCTC	1 cut(s)	345
Eco24I	GRGCYC	5 cut(s)	34, 245, 347, 998, 1017
Eco31I	GGTCTC	2 cut(s)	64, 1415
Eco47I	GGWCC	2 cut(s)	583, 1316
Eco53kI	GAGCTC	1 cut(s)	345
Eco57I	CTGAAG	1 cut(s)	725
Eco72I	CACGTG	1 cut(s)	712
Eco81I	CCTNAGG	1 cut(s)	695
Eco88I	CYCGRG	3 cut(s)	249, 292, 761
EcoICRI	GAGCTC	1 cut(s)	345
EcoO109I	RGGNCCY	3 cut(s)	104, 583, 1013
EcoRII	CCWGG	3 cut(s)	26, 1309, 1476
EcoT22I	ATGCAT	1 cut(s)	1216
EcoT38I	GRGCYC	5 cut(s)	34, 245, 347, 998, 1017
Esp3I	CGTCTC	2 cut(s)	265, 881
FaeI	CATG	7 cut(s)	136, 568, 848, 968, 1109, 1218, 1429
FalI	AAGNNNNNCTT	2 cut(s)	1341, 1373
FatI	CATG	7 cut(s)	132, 564, 844, 964, 1105, 1214, 1425
FauI	CCCGC	8 cut(s)	419, 679, 829, 851, 912, 944, 1047, 1165
FblI	GTMKAC	3 cut(s)	176, 453, 515
Fnu4HI	GCNGC	6 cut(s)	78, 749, 758, 770, 794, 1494
FokI	GGATG	8 cut(s)	418, 575, 653, 822, 1013, 1052, 1357, 1472
FriOI	GRGCYC	5 cut(s)	34, 245, 347, 998, 1017
Fsp4HI	GCNGC	6 cut(s)	78, 749, 758, 770, 794, 1494
FspBI	CTAG	2 cut(s)	900, 1586
GlaI	GCGC	6 cut(s)	81, 83, 735, 756, 855, 857
GluI	GCNGC	6 cut(s)	78, 749, 758, 770, 794, 1494
GsaI	CCCAGC	1 cut(s)	1378
HaeIII	GGCC	7 cut(s)	106, 198, 548, 639, 1015, 1120, 1309
HgaI	GACGC	6 cut(s)	247, 278, 489, 501, 898, 982
HhaI	GCGC	6 cut(s)	82, 84, 736, 757, 856, 858
Hin1I	GRCGYC	4 cut(s)	270, 512, 807, 974
Hin1II	CATG	7 cut(s)	136, 568, 848, 968, 1109, 1218, 1429
Hin6I	GCGC	6 cut(s)	80, 82, 734, 755, 854, 856
HinP1I	GCGC	6 cut(s)	80, 82, 734, 755, 854, 856
HincII	GTYRAC	2 cut(s)	177, 516
HindII	GTYRAC	2 cut(s)	177, 516
HinfI	GANTC	9 cut(s)	212, 252, 284, 313, 517, 620, 1130, 1328, 1517
HphI	GGTGA	4 cut(s)	16, 796, 1303, 1540
Hpy166II	GTNNAC	7 cut(s)	177, 320, 454, 516, 541, 883, 1561
Hpy188I	TCNGA	4 cut(s)	289, 349, 606, 1342
Hpy188III	TCNNGA	9 cut(s)	317, 626, 763, 871, 1198, 1231, 1262, 1274, 1286
Hpy8I	GTNNAC	7 cut(s)	177, 320, 454, 516, 541, 883, 1561
Hpy99I	CGWCG	9 cut(s)	131, 197, 272, 517, 809, 833, 844, 944, 976
HpyAV	CCTTC	1 cut(s)	868
HpyCH4III	ACNGT	7 cut(s)	118, 418, 560, 1099, 1165, 1271, 1432
HpyCH4IV	ACGT	8 cut(s)	537, 551, 573, 711, 740, 807, 849, 1392
HpyCH4V	TGCA	4 cut(s)	1214, 1370, 1463, 1523
HpyF3I	CTNAG	8 cut(s)	378, 519, 603, 695, 893, 1304, 1339, 1593
HpySE526I	ACGT	8 cut(s)	537, 551, 573, 711, 740, 807, 849, 1392
Hsp92I	GRCGYC	4 cut(s)	270, 512, 807, 974
Hsp92II	CATG	7 cut(s)	136, 568, 848, 968, 1109, 1218, 1429
HspAI	GCGC	6 cut(s)	80, 82, 734, 755, 854, 856
KpnI	GGTACC	1 cut(s)	1098
KroI	GCCGGC	1 cut(s)	508
KroNI	GCCGGC	1 cut(s)	510
Kzo9I	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
LguI	GCTCTTC	2 cut(s)	686, 1483
LmnI	GCTCC	5 cut(s)	149, 350, 814, 1508, 1583
Lsp1109I	GCAGC	3 cut(s)	756, 805, 1480
LweI	GCATC	3 cut(s)	1201, 1379, 1450
MaeI	CTAG	2 cut(s)	900, 1586
MaeII	ACGT	8 cut(s)	537, 551, 573, 711, 740, 807, 849, 1392
MaeIII	GTNAC	5 cut(s)	22, 296, 552, 1291, 1528
MalI	GATC	5 cut(s)	614, 788, 1007, 1103, 1389
MbiI	CCGCTC	2 cut(s)	144, 426
MboI	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
MfeI	CAATTG	1 cut(s)	1524
MhlI	GDGCHC	6 cut(s)	34, 245, 347, 948, 998, 1017
MluCI	AATT	6 cut(s)	68, 719, 1136, 1180, 1234, 1524
MlyI	GAGTC	5 cut(s)	261, 278, 511, 614, 1526
MmeI	TCCRAC	1 cut(s)	312
Mph1103I	ATGCAT	1 cut(s)	1216
MroNI	GCCGGC	1 cut(s)	508
MroXI	GAANNNNTTC	5 cut(s)	371, 530, 719, 1281, 1472
MslI	CAYNNNNRTG	1 cut(s)	1430
MspA1I	CMGCKG	1 cut(s)	796
MunI	CAATTG	1 cut(s)	1524
Mva1269I	GAATGC	1 cut(s)	1144
MvaI	CCWGG	3 cut(s)	28, 1311, 1478
MvnI	CGCG	8 cut(s)	82, 258, 500, 734, 755, 757, 856, 858
NaeI	GCCGGC	1 cut(s)	510
NciI	CCSGG	7 cut(s)	10, 240, 293, 294, 356, 460, 998
NdeII	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
NgoMIV	GCCGGC	1 cut(s)	508
NheI	GCTAGC	1 cut(s)	899
NlaIII	CATG	7 cut(s)	136, 568, 848, 968, 1109, 1218, 1429
NlaIV	GGNNCC	8 cut(s)	360, 562, 1014, 1015, 1059, 1096, 1318, 1475
NmeAIII	GCCGAG	4 cut(s)	224, 524, 1017, 1580
NmuCI	GTSAC	4 cut(s)	22, 296, 552, 1291
NsiI	ATGCAT	1 cut(s)	1216
PaeR7I	CTCGAG	1 cut(s)	249
PauI	GCGCGC	2 cut(s)	80, 854
PciSI	GCTCTTC	2 cut(s)	686, 1483
PcsI	WCGNNNNNNNCGW	8 cut(s)	308, 336, 345, 477, 483, 521, 989, 1014
PctI	GAATGC	1 cut(s)	1144
PdiI	GCCGGC	1 cut(s)	510
PdmI	GAANNNNTTC	5 cut(s)	371, 530, 719, 1281, 1472
PfeI	GAWTC	4 cut(s)	212, 313, 1130, 1328
PfoI	TCCNGGA	1 cut(s)	1476
PinAI	ACCGGT	3 cut(s)	489, 528, 1041
PkrI	GCNGC	6 cut(s)	79, 750, 759, 771, 795, 1495
PleI	GAGTC	5 cut(s)	260, 278, 511, 614, 1525
PmaCI	CACGTG	1 cut(s)	712
PmlI	CACGTG	1 cut(s)	712
PpsI	GAGTC	5 cut(s)	260, 278, 511, 614, 1525
Ppu21I	YACGTR	1 cut(s)	712
PpuMI	RGGWCCY	1 cut(s)	583
PshAI	GACNNNNGTC	1 cut(s)	294
Psp124BI	GAGCTC	1 cut(s)	347
Psp5II	RGGWCCY	1 cut(s)	583
Psp6I	CCWGG	3 cut(s)	26, 1309, 1476
PspCI	CACGTG	1 cut(s)	712
PspFI	CCCAGC	1 cut(s)	1374
PspGI	CCWGG	3 cut(s)	26, 1309, 1476
PspN4I	GGNNCC	8 cut(s)	360, 562, 1014, 1015, 1059, 1096, 1318, 1475
PspOMI	GGGCCC	1 cut(s)	1013
PspPI	GGNCC	6 cut(s)	104, 583, 1013, 1014, 1118, 1316
PspPPI	RGGWCCY	1 cut(s)	583
PspXI	VCTCGAGB	1 cut(s)	249
PsrI	GAACNNNNNNTAC	2 cut(s)	518, 550
PteI	GCGCGC	2 cut(s)	80, 854
RsaI	GTAC	5 cut(s)	800, 884, 1096, 1225, 1255
RsaNI	GTAC	5 cut(s)	799, 883, 1095, 1224, 1254
RseI	CAYNNNNRTG	1 cut(s)	1430
SacI	GAGCTC	1 cut(s)	347
SalI	GTCGAC	2 cut(s)	175, 514
SapI	GCTCTTC	2 cut(s)	686, 1483
SatI	GCNGC	6 cut(s)	78, 749, 758, 770, 794, 1494
Sau3AI	GATC	5 cut(s)	612, 786, 1005, 1101, 1387
Sau96I	GGNCC	6 cut(s)	104, 583, 1013, 1014, 1118, 1316
ScaI	AGTACT	1 cut(s)	1255
SchI	GAGTC	5 cut(s)	261, 278, 511, 614, 1526
SduI	GDGCHC	6 cut(s)	34, 245, 347, 948, 998, 1017
SfaNI	GCATC	3 cut(s)	1201, 1379, 1450
Sfr274I	CTCGAG	1 cut(s)	249
SinI	GGWCC	2 cut(s)	583, 1316
SlaI	CTCGAG	1 cut(s)	249
SmaI	CCCGGG	1 cut(s)	294
SmiMI	CAYNNNNRTG	1 cut(s)	1430
SmlI	CTYRAG	2 cut(s)	170, 249
SmoI	CTYRAG	2 cut(s)	170, 249
Sse9I	AATT	6 cut(s)	68, 719, 1136, 1180, 1234, 1524
SspI	AATATT	1 cut(s)	1414
SspMI	CTAG	2 cut(s)	900, 1586
SstI	GAGCTC	1 cut(s)	347
TaaI	ACNGT	7 cut(s)	118, 418, 560, 1099, 1165, 1271, 1432
TaiI	ACGT	8 cut(s)	540, 554, 576, 714, 743, 810, 852, 1395
TasI	AATT	6 cut(s)	68, 719, 1136, 1180, 1234, 1524
TatI	WGTACW	1 cut(s)	1253
TauI	GCSGC	3 cut(s)	80, 751, 760
TfiI	GAWTC	4 cut(s)	212, 313, 1130, 1328
TscAI	CASTG	5 cut(s)	423, 914, 1308, 1437, 1470
TseFI	GTSAC	4 cut(s)	22, 296, 552, 1291
TseI	GCWGC	3 cut(s)	769, 793, 1493
Tsp45I	GTSAC	4 cut(s)	22, 296, 552, 1291
TspDTI	ATGAA	3 cut(s)	61, 268, 380
TspGWI	ACGGA	3 cut(s)	217, 493, 828
TspMI	CCCGGG	1 cut(s)	292
TspRI	CASTG	5 cut(s)	423, 914, 1308, 1437, 1470
VpaK11BI	GGWCC	2 cut(s)	583, 1316
XapI	RAATTY	1 cut(s)	719
XhoI	CTCGAG	1 cut(s)	249
XmaI	CCCGGG	1 cut(s)	292
XmiI	GTMKAC	3 cut(s)	176, 453, 515
XmnI	GAANNNNTTC	5 cut(s)	371, 530, 719, 1281, 1472
XspI	CTAG	2 cut(s)	900, 1586
ZraI	GACGTC	1 cut(s)	808
ZrmI	AGTACT	1 cut(s)	1255
Zsp2I	ATGCAT	1 cut(s)	1216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.