MD03G1149700.v1.1

Cold-inducible RNA-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0020921
Physical Location & Seq
Forward (+)
16649261 .. 16651097
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1149700.v1.1.491

Sequence Viewer

Length: 363 bp
ATGGCGGCTTCGTTAGGAGGCCGAATGTCCAATAGCTTTCTCAGGAACGCCTCACATTCCAACTCCTATATCTCTCCTGCCCACTTTCTCTTCTCGAGGGGATTTTCTTCCAAGCTTTTCGTCAAAGGTATATCATTTGCCACCACTGAAAAATCATTAACTGAAGCATTTTCGCCATTTGGAAAGGTCGTTCATGTTCACATATTGCGAAATGCAACTACAAACAAGTCAAAAGGATTTGGATATGTGACCTTTGAAGGAGAAGAGGAGGCCCAGAACGCTCTGACGAACATGAATGGGAAGATGGTGGATCAACGCGTTATTTTCGTGGACAAAGCAGGGCGCTACACTCGAGGGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

121

Amino Acids

13.19

Weight (kDa)

10.07

Isoelectric Point (pI)

35.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 39 - 108 2.2e-21 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27330
malus_domestica MD03G1149700.v1.1
prunus_persica Prupe.6G135700_v2.0.a1 Prupe.6G135700_v2.0.a1 Prupe.6G135700_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 318
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 316
AgsI TTSAA 1 cut(s) 257
AloI GAACNNNNNNTCC 2 cut(s) 174, 206
AluBI AGCT 2 cut(s) 36, 115
AluI AGCT 2 cut(s) 36, 115
AlwI GGATC 1 cut(s) 318
Ama87I CYCGRG 2 cut(s) 94, 351
AoxI GGCC 2 cut(s) 19, 270
AspLEI GCGC 1 cut(s) 345
AspS9I GGNCC 1 cut(s) 271
AvaI CYCGRG 2 cut(s) 94, 351
BccI CCATC 1 cut(s) 298
BfoI RGCGCY 1 cut(s) 346
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmeT110I CYCGRG 2 cut(s) 94, 351
BmgT120I GGNCC 1 cut(s) 271
BseMII CTCAG 1 cut(s) 55
BseRI GAGGAG 1 cut(s) 281
Bsh1236I CGCG 1 cut(s) 318
BshFI GGCC 2 cut(s) 21, 272
BsiHKCI CYCGRG 2 cut(s) 94, 351
BsnI GGCC 2 cut(s) 21, 272
BsoBI CYCGRG 2 cut(s) 94, 351
Bsp143I GATC 1 cut(s) 310
BspACI CCGC 1 cut(s) 5
BspANI GGCC 2 cut(s) 21, 272
BspCNI CTCAG 1 cut(s) 54
BspFNI CGCG 1 cut(s) 318
BspPI GGATC 1 cut(s) 318
BssMI GATC 1 cut(s) 310
Bst6I CTCTTC 2 cut(s) 95, 258
BstDEI CTNAG 1 cut(s) 41
BstFNI CGCG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 1 cut(s) 345
BstKTI GATC 1 cut(s) 313
BstMBI GATC 1 cut(s) 310
BstMWI GCNNNNNNNGC 1 cut(s) 278
BstUI CGCG 1 cut(s) 318
BsuRI GGCC 2 cut(s) 21, 272
BtsIMutI CAGTG 1 cut(s) 144
CfoI GCGC 1 cut(s) 345
Cfr13I GGNCC 1 cut(s) 271
CviAII CATG 2 cut(s) 194, 292
CviJI RGCY 5 cut(s) 8, 21, 36, 115, 272
CviKI_1 RGCY 5 cut(s) 8, 21, 36, 115, 272
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 312
DpnII GATC 1 cut(s) 310
Eam1104I CTCTTC 2 cut(s) 95, 258
EarI CTCTTC 2 cut(s) 95, 258
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 2 cut(s) 94, 351
FaeI CATG 2 cut(s) 197, 295
FaiI YATR 6 cut(s) 69, 131, 195, 203, 246, 293
FatI CATG 2 cut(s) 193, 291
Fnu4HI GCNGC 1 cut(s) 6
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 344
GluI GCNGC 1 cut(s) 6
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 2 cut(s) 21, 272
HhaI GCGC 1 cut(s) 345
Hin1II CATG 2 cut(s) 197, 295
Hin6I GCGC 1 cut(s) 343
HinP1I GCGC 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 113
Hpy166II GTNNAC 2 cut(s) 199, 331
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 2 cut(s) 43, 94
Hpy8I GTNNAC 2 cut(s) 199, 331
HpyAV CCTTC 1 cut(s) 251
HpyCH4V TGCA 1 cut(s) 215
HpyF10VI GCNNNNNNNGC 1 cut(s) 278
HpyF3I CTNAG 1 cut(s) 41
Hsp92II CATG 2 cut(s) 197, 295
HspAI GCGC 1 cut(s) 343
Kzo9I GATC 1 cut(s) 310
LpnPI CCDG 4 cut(s) 28, 90, 287, 324
MaeIII GTNAC 1 cut(s) 247
MalI GATC 1 cut(s) 312
MboI GATC 1 cut(s) 310
MboII GAAGA 4 cut(s) 82, 99, 275, 313
MluI ACGCGT 1 cut(s) 316
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 6 cut(s) 11, 61, 90, 259, 262, 347
MseI TTAA 1 cut(s) 158
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 1 cut(s) 278
NdeII GATC 1 cut(s) 310
NlaIII CATG 2 cut(s) 197, 295
NmuCI GTSAC 1 cut(s) 247
PaeR7I CTCGAG 2 cut(s) 94, 351
PkrI GCNGC 1 cut(s) 7
PspPI GGNCC 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 351
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 1 cut(s) 310
Sau96I GGNCC 1 cut(s) 271
SetI ASST 5 cut(s) 38, 117, 130, 189, 254
Sfr274I CTCGAG 2 cut(s) 94, 351
SlaI CTCGAG 2 cut(s) 94, 351
SmlI CTYRAG 2 cut(s) 94, 351
SmoI CTYRAG 2 cut(s) 94, 351
SsiI CCGC 1 cut(s) 5
TaqI TCGA 2 cut(s) 95, 352
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 247
Tsp45I GTSAC 1 cut(s) 247
TspDTI ATGAA 2 cut(s) 182, 308
TspRI CASTG 1 cut(s) 151
XhoI CTCGAG 2 cut(s) 94, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.