MD03G1177000.v1.1

1-acyl-sn-glycerol-3-phosphate acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0024324
Physical Location & Seq
Forward (+)
24324875 .. 24331301
6427 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1177000.v1.1.491

Sequence Viewer

Length: 1137 bp
ATGGAGGTTAGAGCTGTATTGTTCTCAATTCCTTTTGGACTGACCTTTCTTCTCTTTGGTCTAGCTATTAATCTTATCCAGGCGATTTGCTTCCTCACCATTCGGCCTTTGTCGAAGAGTGCGTTTAGACAGATCAATGGAGCAGTTGCATCATTCTTATGGTTGGATCTCATTTGGCTCATGGAGTGGTGGGGAGGCCTTAAGGTTAAGTTGTACACAGATTTGGAAACTTATCGACTAATGGGTAAGGAGCATGCGTTGCTCATGCCAAACCACATCTGTGATGCTGATATACTGATGGGGTTGCTTTTAGCTAATCGCTCGGGTTGCCTTCGTAGTGCGCTTATGGTTGTCAAGAAATCTTCAAAATACCTTCCTGTTTTTGGGTGGACTTCTTGGTTCCTAGAGTTCGTTTTCCTCGATAGAAGCTGGGAAAAAGATGAAAGCAACCTAAAGGCAAGTTTACAGAGGTTAAAGGATTTTACCATGCCTTTTTGGCTTACCATTTTTGCGGAAGGAACACGCATGAGTCCAGACAAGCTTTCTGCAGCTCGAGAGTTTGCTGCTTCTAGAAAATTGCCTATCCCTAGAAATGTCATGATTCCTAGGACTAAGGGTTTTGTTACAGCAGTAAAAAATTTGCGCTCGTTTGTCCCTGCAATCTATGATGTTACTCTCGCTGTTCCGGAAGGACATTCTACTCCTTCACTATATATGCTAATGGAGAGGCAACATACAGTGGTCAATGTACATGTAAAGCGATACTCAATGACTGAATTGCCAGAATCAGACGAGGGCATTGCCCAATGGTGCAGAGATAGATTTGTGGCCAAGGATTCCTTGTTGGACAAGTTTAAAGCTGAGGGTACCTTTCCCGGCCAAGAAATTCATGACACTGGTCGATCTATTAAGGCATTGCTCGCGTATACGAGCAGTTTTTGTACACTCTGCCTTGCGATTTTCGAGCTGTTCCAAAAGTTCTCTCTTTTATACACATGGGGAGGGGTGGGCTTGTTAACAGCTTCTTTTCTTACCATTATGCTCATCATCCATATTTTCATCGAATACACAAAGTTGCCGAAACAAAACGGAGTTAAGACCACTCCATCACGAAAAGGTCAAGCTAAAGAGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko00564 ko01100 ko01110 ko04072 map00561 map00564 map01100 map01110 map04072
Pfam Domains
Protein Families
Acyltransf_C Acyltransferase

Protein Analysis

379

Amino Acids

43.07

Weight (kDa)

9.32

Isoelectric Point (pI)

38.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Acyltransferase PF01553 83 - 224 1.5e-11 Acyltransferase
Acyltransf_C PF16076 240 - 308 1.1e-21 Acyltransferase C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024324)

Species Orthologous Gene IDs
malus_domestica MD03G1177000.v1.1
prunus_persica Prupe.4G245800_v2.0.a1 Prupe.4G245800_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 866
AccB1I GGYRCC 1 cut(s) 866
AccI GTMKAC 1 cut(s) 926
AccII CGCG 1 cut(s) 923
AccIII TCCGGA 1 cut(s) 685
AciI CCGC 1 cut(s) 512
AclWI GGATC 1 cut(s) 174
AcoI YGGCCR 2 cut(s) 828, 877
AcsI RAATTY 2 cut(s) 637, 885
AfaI GTAC 4 cut(s) 215, 750, 868, 943
AfiI CCNNNNNNNGG 1 cut(s) 383
AflII CTTAAG 1 cut(s) 200
AflIII ACRYGT 1 cut(s) 751
AgsI TTSAA 1 cut(s) 366
AjnI CCWGG 1 cut(s) 78
AleI CACNNNNGTG 1 cut(s) 279
AlwI GGATC 1 cut(s) 174
Ama87I CYCGRG 2 cut(s) 322, 552
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 4 cut(s) 104, 196, 828, 877
ApeKI GCWGC 2 cut(s) 548, 563
ApoI RAATTY 2 cut(s) 637, 885
AseI ATTAAT 1 cut(s) 69
Asp718I GGTACC 1 cut(s) 866
AspA2I CCTAGG 1 cut(s) 605
AspLEI GCGC 2 cut(s) 343, 645
AsuC2I CCSGG 1 cut(s) 876
AsuHPI GGTGA 1 cut(s) 88
AvaI CYCGRG 2 cut(s) 322, 552
AvrII CCTAGG 1 cut(s) 605
BalI TGGCCA 1 cut(s) 830
BanI GGYRCC 1 cut(s) 866
BbvCI CCTCAGC 1 cut(s) 861
BbvI GCAGC 2 cut(s) 550, 560
BccI CCATC 2 cut(s) 292, 1114
BcgI CGANNNNNNTGC 2 cut(s) 782, 816
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 876
BfaI CTAG 5 cut(s) 62, 404, 570, 588, 606
BfmI CTRYAG 1 cut(s) 546
BfrI CTTAAG 1 cut(s) 200
BglI GCCNNNNNGGC 1 cut(s) 496
BisI GCNGC 2 cut(s) 549, 564
BlnI CCTAGG 1 cut(s) 605
BlsI GCNGC 2 cut(s) 550, 565
Bme1390I CCNGG 2 cut(s) 80, 876
BmeT110I CYCGRG 2 cut(s) 322, 552
BmiI GGNNCC 2 cut(s) 401, 868
BmrFI CCNGG 2 cut(s) 80, 876
BmsI GCATC 2 cut(s) 158, 274
BoxI GACNNNNGTC 1 cut(s) 897
Bpu10I CCTNAGC 1 cut(s) 861
BpuMI CCSGG 1 cut(s) 876
BsaJI CCNNGG 2 cut(s) 605, 831
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 2 cut(s) 242, 272
Bsc4I CCNNNNNNNGG 1 cut(s) 383
Bse1I ACTGG 1 cut(s) 901
Bse3DI GCAATG 2 cut(s) 798, 914
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 2 cut(s) 605, 831
BseGI GGATG 1 cut(s) 1047
BseLI CCNNNNNNNGG 1 cut(s) 383
BseMI GCAATG 2 cut(s) 798, 914
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 1 cut(s) 901
BseXI GCAGC 2 cut(s) 550, 560
BseYI CCCAGC 1 cut(s) 429
BsgI GTGCAG 1 cut(s) 832
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 4 cut(s) 106, 198, 830, 879
BshNI GGYRCC 1 cut(s) 866
BsiHKCI CYCGRG 2 cut(s) 322, 552
BsiSI CCGG 2 cut(s) 686, 876
BslFI GGGAC 1 cut(s) 638
BslI CCNNNNNNNGG 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 638
BsnI GGCC 4 cut(s) 106, 198, 830, 879
BsoBI CYCGRG 2 cut(s) 322, 552
Bsp13I TCCGGA 1 cut(s) 685
Bsp1407I TGTACA 3 cut(s) 213, 748, 941
Bsp143I GATC 3 cut(s) 132, 166, 902
BspACI CCGC 1 cut(s) 512
BspANI GGCC 4 cut(s) 106, 198, 830, 879
BspCNI CTCAG 1 cut(s) 853
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 1 cut(s) 923
BspHI TCATGA 2 cut(s) 597, 889
BspLI GGNNCC 2 cut(s) 401, 868
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 1 cut(s) 174
BspT107I GGYRCC 1 cut(s) 866
BspTI CTTAAG 1 cut(s) 200
BsrDI GCAATG 2 cut(s) 798, 914
BsrGI TGTACA 3 cut(s) 213, 748, 941
BsrI ACTGG 1 cut(s) 901
BssECI CCNNGG 2 cut(s) 605, 831
BssMI GATC 3 cut(s) 132, 166, 902
BssNAI GTATAC 1 cut(s) 927
BssT1I CCWWGG 2 cut(s) 605, 831
Bst1107I GTATAC 1 cut(s) 927
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 1 cut(s) 739
Bst6I CTCTTC 1 cut(s) 110
BstAFI CTTAAG 1 cut(s) 200
BstAPI GCANNNNNTGC 1 cut(s) 259
BstAUI TGTACA 3 cut(s) 213, 748, 941
BstC8I GCNNGC 2 cut(s) 255, 921
BstDEI CTNAG 2 cut(s) 612, 861
BstF5I GGATG 1 cut(s) 1047
BstFNI CGCG 1 cut(s) 923
BstHHI GCGC 2 cut(s) 343, 645
BstKTI GATC 3 cut(s) 135, 169, 905
BstMBI GATC 3 cut(s) 132, 166, 902
BstMWI GCNNNNNNNGC 4 cut(s) 259, 327, 496, 920
BstNI CCWGG 1 cut(s) 80
BstNSI RCATGY 2 cut(s) 257, 755
BstPAI GACNNNNGTC 1 cut(s) 897
BstSCI CCNGG 2 cut(s) 78, 874
BstSFI CTRYAG 1 cut(s) 546
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 2 cut(s) 550, 560
BstX2I RGATCY 1 cut(s) 166
BstYI RGATCY 1 cut(s) 166
BstZ17I GTATAC 1 cut(s) 927
BsuRI GGCC 4 cut(s) 106, 198, 830, 879
BtsCI GGATG 1 cut(s) 1047
BtsIMutI CAGTG 2 cut(s) 744, 894
Cac8I GCNNGC 2 cut(s) 255, 921
CciI TCATGA 2 cut(s) 597, 889
CfoI GCGC 2 cut(s) 343, 645
Csp6I GTAC 4 cut(s) 214, 749, 867, 942
CviAII CATG 9 cut(s) 181, 254, 265, 487, 526, 598, 752, 890, 996
CviQI GTAC 4 cut(s) 214, 749, 867, 942
DdeI CTNAG 2 cut(s) 612, 861
DpnI GATC 3 cut(s) 134, 168, 904
DpnII GATC 3 cut(s) 132, 166, 902
DraI TTTAAA 1 cut(s) 856
EaeI YGGCCR 2 cut(s) 828, 877
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 2 cut(s) 605, 831
Eco147I AGGCCT 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 322, 552
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 2 cut(s) 605, 831
ErhI CCWWGG 2 cut(s) 605, 831
FaeI CATG 9 cut(s) 184, 257, 268, 490, 529, 601, 755, 893, 999
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FaqI GGGAC 1 cut(s) 638
FatI CATG 9 cut(s) 180, 253, 264, 486, 525, 597, 751, 889, 995
FblI GTMKAC 1 cut(s) 926
Fnu4HI GCNGC 2 cut(s) 549, 564
FokI GGATG 1 cut(s) 1034
Fsp4HI GCNGC 2 cut(s) 549, 564
FspBI CTAG 5 cut(s) 62, 404, 570, 588, 606
GlaI GCGC 2 cut(s) 342, 644
GluI GCNGC 2 cut(s) 549, 564
GsaI CCCAGC 1 cut(s) 433
HaeIII GGCC 4 cut(s) 106, 198, 830, 879
HapII CCGG 2 cut(s) 686, 876
HhaI GCGC 2 cut(s) 343, 645
Hin1II CATG 9 cut(s) 184, 257, 268, 490, 529, 601, 755, 893, 999
Hin6I GCGC 2 cut(s) 341, 643
HinP1I GCGC 2 cut(s) 341, 643
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HindIII AAGCTT 1 cut(s) 539
HinfI GANTC 4 cut(s) 529, 601, 785, 836
HpaI GTTAAC 1 cut(s) 1017
HpaII CCGG 2 cut(s) 686, 876
HphI GGTGA 1 cut(s) 88
Hpy166II GTNNAC 6 cut(s) 216, 390, 464, 927, 944, 1017
Hpy188I TCNGA 1 cut(s) 790
Hpy188III TCNNGA 8 cut(s) 355, 533, 554, 570, 598, 686, 890, 1110
Hpy8I GTNNAC 6 cut(s) 216, 390, 464, 927, 944, 1017
HpyAV CCTTC 5 cut(s) 341, 383, 509, 683, 714
HpyCH4III ACNGT 1 cut(s) 739
HpyCH4V TGCA 4 cut(s) 149, 548, 659, 813
HpyF10VI GCNNNNNNNGC 4 cut(s) 259, 327, 496, 920
HpyF3I CTNAG 2 cut(s) 612, 861
Hsp92II CATG 9 cut(s) 184, 257, 268, 490, 529, 601, 755, 893, 999
HspAI GCGC 2 cut(s) 341, 643
Kpn2I TCCGGA 1 cut(s) 685
KpnI GGTACC 1 cut(s) 870
KspAI GTTAAC 1 cut(s) 1017
Kzo9I GATC 3 cut(s) 132, 166, 902
LmnI GCTCC 2 cut(s) 140, 250
Lsp1109I GCAGC 2 cut(s) 550, 560
LweI GCATC 2 cut(s) 158, 274
MaeI CTAG 5 cut(s) 62, 404, 570, 588, 606
MaeIII GTNAC 2 cut(s) 622, 670
MalI GATC 3 cut(s) 134, 168, 904
MboI GATC 3 cut(s) 132, 166, 902
MboII GAAGA 3 cut(s) 41, 127, 354
MflI RGATCY 1 cut(s) 166
MlsI TGGCCA 1 cut(s) 830
MluCI AATT 5 cut(s) 27, 575, 637, 776, 885
MluNI TGGCCA 1 cut(s) 830
MlyI GAGTC 1 cut(s) 538
MmeI TCCRAC 2 cut(s) 144, 825
MnlI CCTC 9 cut(s) 104, 188, 428, 462, 720, 787, 856, 995, 1123
Mox20I TGGCCA 1 cut(s) 830
MroI TCCGGA 1 cut(s) 685
MscI TGGCCA 1 cut(s) 830
MseI TTAA 8 cut(s) 69, 201, 207, 473, 855, 909, 1016, 1095
MslI CAYNNNNRTG 2 cut(s) 157, 279
Msp20I TGGCCA 1 cut(s) 830
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 2 cut(s) 686, 876
MspR9I CCNGG 2 cut(s) 80, 876
MvaI CCWGG 1 cut(s) 80
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 4 cut(s) 259, 327, 496, 920
NciI CCSGG 1 cut(s) 876
NdeII GATC 3 cut(s) 132, 166, 902
NlaIII CATG 9 cut(s) 184, 257, 268, 490, 529, 601, 755, 893, 999
NlaIV GGNNCC 2 cut(s) 401, 868
NspI RCATGY 2 cut(s) 257, 755
OliI CACNNNNGTG 1 cut(s) 279
PaeI GCATGC 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 552
PagI TCATGA 2 cut(s) 597, 889
PceI AGGCCT 1 cut(s) 198
PciI ACATGT 1 cut(s) 751
PcsI WCGNNNNNNNCGW 2 cut(s) 119, 417
PfeI GAWTC 3 cut(s) 601, 785, 836
PkrI GCNGC 2 cut(s) 550, 565
PleI GAGTC 1 cut(s) 537
PpsI GAGTC 1 cut(s) 537
PscI ACATGT 1 cut(s) 751
PshAI GACNNNNGTC 1 cut(s) 897
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 1 cut(s) 78
PspFI CCCAGC 1 cut(s) 429
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 401, 868
PstI CTGCAG 1 cut(s) 550
PsuI RGATCY 1 cut(s) 166
RsaI GTAC 4 cut(s) 215, 750, 868, 943
RsaNI GTAC 4 cut(s) 214, 749, 867, 942
RseI CAYNNNNRTG 2 cut(s) 157, 279
SaqAI TTAA 8 cut(s) 69, 201, 207, 473, 855, 909, 1016, 1095
SatI GCNGC 2 cut(s) 549, 564
Sau3AI GATC 3 cut(s) 132, 166, 902
SchI GAGTC 1 cut(s) 538
ScrFI CCNGG 2 cut(s) 80, 876
SfaNI GCATC 2 cut(s) 158, 274
SfcI CTRYAG 1 cut(s) 546
Sfr274I CTCGAG 1 cut(s) 552
SlaI CTCGAG 1 cut(s) 552
SmiMI CAYNNNNRTG 2 cut(s) 157, 279
SmlI CTYRAG 2 cut(s) 200, 552
SmoI CTYRAG 2 cut(s) 200, 552
SphI GCATGC 1 cut(s) 257
Sse9I AATT 5 cut(s) 27, 575, 637, 776, 885
SseBI AGGCCT 1 cut(s) 198
SsiI CCGC 1 cut(s) 512
SspMI CTAG 5 cut(s) 62, 404, 570, 588, 606
StuI AGGCCT 1 cut(s) 198
StyD4I CCNGG 2 cut(s) 78, 874
StyI CCWWGG 2 cut(s) 605, 831
TaaI ACNGT 1 cut(s) 739
TaqI TCGA 7 cut(s) 113, 235, 420, 553, 901, 963, 1062
TasI AATT 5 cut(s) 27, 575, 637, 776, 885
TatI WGTACW 3 cut(s) 213, 748, 941
TfiI GAWTC 3 cut(s) 601, 785, 836
Tru1I TTAA 8 cut(s) 69, 201, 207, 473, 855, 909, 1016, 1095
Tru9I TTAA 8 cut(s) 69, 201, 207, 473, 855, 909, 1016, 1095
TscAI CASTG 2 cut(s) 744, 901
TseI GCWGC 2 cut(s) 548, 563
TspDTI ATGAA 3 cut(s) 456, 878, 1048
TspGWI ACGGA 1 cut(s) 1104
TspRI CASTG 2 cut(s) 744, 901
Vha464I CTTAAG 1 cut(s) 200
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 2 cut(s) 637, 885
XbaI TCTAGA 1 cut(s) 569
XceI RCATGY 2 cut(s) 257, 755
XhoI CTCGAG 1 cut(s) 552
XmaJI CCTAGG 1 cut(s) 605
XmiI GTMKAC 1 cut(s) 926
XspI CTAG 5 cut(s) 62, 404, 570, 588, 606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.