Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006853

Physical Location & Seq

Genomic Coordinates Forward (+)

28809303 .. 28817196

Gene Structure 7894 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1209000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGGCGATTGGGAGTTGCATCTGAGAATCCTTTCAAGCAGTGCCAGAGACTCCAATGTCGCGAACGATCCCGCTTCCGACTCCTCTCTTCTCCAATCCGTGAAGAAGCTGTATGAATTGTGTAAAGCTGAGAATTCAGAGGATTTGGTGGCGAGAGTTTACACGCAGATTAACAAGCTCTTTCAGCGCTCGGTGGCTTCGCTGTCTCAATCTCGAACCTCCATTGGCCTTCTACTGCTGGCCATTCTTCAATTTTACCTTGACTTTGGGGAGATGGTTCTACATGATGCTGACCCCAGTCTCAGGACATTTTTCCGTTCATGTTTGAGCCGTGAGTTTGCAGATCCTGTTGTCGCAGAATCAACCCTTGAATTTCTGAACGTCAACAAGAAAAAGCTTTTAACTTCTTTCCCTACCTTACTGCCTCAATTCTTTCCCTTGATGTTAAAGTTGATTGCATGGAATGGGGAAAAGTTAGAAAAGTCATATATGAAAGTTTTTCCAGGGTTGATTTCGCCTGGGTCATTTCTTCCATTATTTCCAGCTTTGGTAGACTTGCCAATTTTGGTAGTGGCATTGGAGAAGGTTGAAAAGAGCTCGGGATCGTTAGTTGGAAACAGCATTGCATCAATGCAGAAGAGTTCGGCTCCCGAGATGCTGCTCGCTCTTATGGATGAAGCATATACTGGTTCAACAATAGGAGATGGAGGGGGAGACTCAGAATCAGAGGACAGCAGTTCAATTGATGTTGCTGATCCTCTGTTCCTTGACCTTCTAAAGGATGAAAATGATGGCATTGCTGAACGCCATTGGACCTCTCCTGCGATGGCTGCAACTTTACAGGCTGTAATTAATACTCCCCAATCTAATAGGCTGAAAAATATACTACGCATGACACCATGGTTTCTTGATGTGTATTTTGCTATAGCACTGCGTGATGTCAATAATTCTTTGGTCTGTGCATTGATACCTCTACTCATGTCCAGAAATGCCACAATATTTCCTGACAGAAGTTTTTCTTATGAGGTTCGTAAGAGGCTATTAGAATTCATGCTTGCTGCATTTCAGCGCTCTCCAGATTTTATTGCACTTTTGAAGAAGCCTATAATGGACAGGCTAGGGGAAGCTTATGACAGCCCAGAAAAGACAGAGTTGGCATTACAATTGTGCTGGGCCATTGGAGAGCATGGTGGGGGTGGACGATCTCACAAAGATGAAGCTCGCGAGCTTTTTGAGAGTTTAGAGCTACTCTTGTATGAAAACCTTTCATCTAGTCGTCTAGGCCTGGGACAGGAATCAACCTTTAGCTCTGATAATCAAACTTACCGAAAGTCATCACAATCAAGGCTTCTATGCTTTGTTGTTACAGCCATTGCAAAGTTGGCCACCATCCACCGTGAATTGCTGCCTAGGGCACATTTTTCCTTGGGCAAGGTAGCTCGCTCTCGAATATCAGATGAGAGGGTCTGGAGACGGGCTCGTGATTATTTGGGCTTAATGACAGAACCTGCAATTTCCTTGTCTGTCTTGGGGCCTTCCCGGCCTTCAAATGGACAAATGCAGAACCGCGGTACTGTCAAGTGGAGTGATGGTGCATCAAAGATGATTGCACATGTTCCATTTTATATTCTTGGTGAACAAGAAGGTCCACCCTTCCATGATTTTTTGTTCTCGGATATTCTTCCAAGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

SPG48

Protein Analysis

565

Amino Acids

62.9

Weight (kDa)

5.95

Isoelectric Point (pI)

51.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPG48	PF14764	26 - 150	6.3e-37	AP-5 complex subunit, vesicle trafficking
TPR_AP5Z1_C	PF25154	275 - 518	2.5e-25	AP-5 complex subunit zeta-1, C-terminal TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006853)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G15160 AT3G15160
fragaria_vesca	FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170 FvH4_4g07170
malus_domestica	MD03G1209000.v1.1
prunus_persica	Prupe.4G206200_v2.0.a1 Prupe.4G206200_v2.0.a1
pyrus_communis	pycom03g15800
rosa_chinensis	RchiOBHm_Chr4g0399351
rosa_laevigata	RLG00000009273
rosa_roxburghii	Rroxscaffold_5G00344310
rosa_rugosa	Rorug04G0016800
rosa_samantha	Rh4AG094500 Rh4BG091800 Rh4CG102800 Rh4DG086800 Rh7DG335600
rosa_wichuraiana	Rw4G007740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	56
Acc36I	ACCTGC	1 cut(s)	1516
AccI	GTMKAC	1 cut(s)	552
AccII	CGCG	3 cut(s)	62, 1224, 1569
AciI	CCGC	3 cut(s)	72, 1567, 1569
AclWI	GGATC	4 cut(s)	62, 338, 610, 749
AcoI	YGGCCR	2 cut(s)	240, 1383
AcsI	RAATTY	3 cut(s)	133, 371, 1046
AdeI	CACNNNGTG	1 cut(s)	935
AfaI	GTAC	1 cut(s)	1573
AfeI	AGCGCT	2 cut(s)	188, 1070
AfiI	CCNNNNNNNGG	4 cut(s)	303, 778, 1291, 1550
AflIII	ACRYGT	1 cut(s)	1612
AgsI	TTSAA	8 cut(s)	36, 251, 371, 590, 693, 741, 1096, 1548
AjnI	CCWGG	3 cut(s)	502, 517, 1284
Alw21I	GWGCWC	1 cut(s)	599
Alw26I	GTCTC	5 cut(s)	42, 210, 305, 708, 1465
AlwI	GGATC	4 cut(s)	62, 338, 610, 749
AlwNI	CAGNNNCTG	2 cut(s)	347, 1508
Ama87I	CYCGRG	2 cut(s)	598, 650
Aor51HI	AGCGCT	2 cut(s)	188, 1070
AoxI	GGCC	7 cut(s)	226, 240, 1173, 1282, 1383, 1532, 1541
ApeKI	GCWGC	4 cut(s)	658, 830, 1058, 1405
ApoI	RAATTY	3 cut(s)	133, 371, 1046
AseI	ATTAAT	1 cut(s)	852
Asp700I	GAANNNNTTC	2 cut(s)	31, 1015
AspA2I	CCTAGG	1 cut(s)	1409
AspLEI	GCGC	2 cut(s)	189, 1071
AspS9I	GGNCC	4 cut(s)	813, 1173, 1532, 1646
AsuC2I	CCSGG	1 cut(s)	1540
AsuHPI	GGTGA	1 cut(s)	1646
AvaI	CYCGRG	2 cut(s)	598, 650
AvaII	GGWCC	2 cut(s)	813, 1646
AvrII	CCTAGG	1 cut(s)	1409
BaeGI	GKGCMC	1 cut(s)	1417
BalI	TGGCCA	2 cut(s)	242, 1385
BanII	GRGCYC	2 cut(s)	599, 1480
BauI	CACGAG	1 cut(s)	1479
Bbv12I	GWGCWC	1 cut(s)	599
BbvI	GCAGC	4 cut(s)	645, 817, 1045, 1392
BccI	CCATC	6 cut(s)	268, 698, 785, 820, 1397, 1583
BceAI	ACGGC	1 cut(s)	315
BciT130I	CCWGG	3 cut(s)	504, 519, 1286
BcnI	CCSGG	1 cut(s)	1540
BcoDI	GTCTC	5 cut(s)	42, 210, 305, 708, 1465
BfaI	CTAG	4 cut(s)	1118, 1272, 1280, 1410
BfmI	CTRYAG	1 cut(s)	924
BfoI	RGCGCY	2 cut(s)	190, 1072
BfuAI	ACCTGC	1 cut(s)	1516
BglI	GCCNNNNNGGC	1 cut(s)	1540
BisI	GCNGC	4 cut(s)	659, 831, 1059, 1406
BlnI	CCTAGG	1 cut(s)	1409
BlsI	GCNGC	4 cut(s)	660, 832, 1060, 1407
Bme1390I	CCNGG	4 cut(s)	504, 519, 1286, 1540
Bme18I	GGWCC	2 cut(s)	813, 1646
BmeT110I	CYCGRG	2 cut(s)	598, 650
BmgT120I	GGNCC	4 cut(s)	813, 1173, 1532, 1646
BmiI	GGNNCC	2 cut(s)	648, 1533
BmrFI	CCNGG	4 cut(s)	504, 519, 1286, 1540
BmrI	ACTGGG	1 cut(s)	291
BmsI	GCATC	5 cut(s)	28, 277, 635, 645, 1604
BmuI	ACTGGG	1 cut(s)	291
BoxI	GACNNNNGTC	1 cut(s)	297
BplI	GAGNNNNNCTC	4 cut(s)	631, 663, 1462, 1494
BpmI	CTGGAG	2 cut(s)	1059, 1489
BpuMI	CCSGG	1 cut(s)	1540
BsaJI	CCNNGG	7 cut(s)	503, 518, 899, 1285, 1409, 1425, 1567
BsaXI	ACNNNNNCTCC	2 cut(s)	1576, 1606
Bsc4I	CCNNNNNNNGG	4 cut(s)	303, 778, 1291, 1550
Bse1I	ACTGG	2 cut(s)	297, 691
Bse3DI	GCAATG	3 cut(s)	621, 795, 1371
BseBI	CCWGG	3 cut(s)	504, 519, 1286
BseDI	CCNNGG	7 cut(s)	503, 518, 899, 1285, 1409, 1425, 1567
BseGI	GGATG	3 cut(s)	679, 787, 1389
BseLI	CCNNNNNNNGG	4 cut(s)	303, 778, 1291, 1550
BseMI	GCAATG	3 cut(s)	621, 795, 1371
BseMII	CTCAG	4 cut(s)	14, 120, 316, 732
BseNI	ACTGG	2 cut(s)	297, 691
BseRI	GAGGAG	1 cut(s)	73
BseSI	GKGCMC	1 cut(s)	1417
BseXI	GCAGC	4 cut(s)	645, 817, 1045, 1392
BseYI	CCCAGC	1 cut(s)	1170
Bsh1236I	CGCG	3 cut(s)	62, 1224, 1569
BshFI	GGCC	7 cut(s)	228, 242, 1175, 1284, 1385, 1534, 1543
BsiHKAI	GWGCWC	1 cut(s)	599
BsiHKCI	CYCGRG	2 cut(s)	598, 650
BsiSI	CCGG	1 cut(s)	1540
BslFI	GGGAC	1 cut(s)	1302
BslI	CCNNNNNNNGG	4 cut(s)	303, 778, 1291, 1550
BsmAI	GTCTC	5 cut(s)	42, 210, 305, 708, 1465
BsmBI	CGTCTC	1 cut(s)	1465
BsmFI	GGGAC	1 cut(s)	1302
BsnI	GGCC	7 cut(s)	228, 242, 1175, 1284, 1385, 1534, 1543
BsoBI	CYCGRG	2 cut(s)	598, 650
Bsp1286I	GDGCHC	3 cut(s)	599, 1417, 1480
Bsp143I	GATC	5 cut(s)	67, 343, 602, 754, 1202
Bsp19I	CCATGG	1 cut(s)	899
Bsp68I	TCGCGA	2 cut(s)	62, 1224
BspACI	CCGC	3 cut(s)	72, 1567, 1569
BspANI	GGCC	7 cut(s)	228, 242, 1175, 1284, 1385, 1534, 1543
BspCNI	CTCAG	4 cut(s)	15, 121, 315, 731
BspFNI	CGCG	3 cut(s)	62, 1224, 1569
BspLI	GGNNCC	2 cut(s)	648, 1533
BspMI	ACCTGC	1 cut(s)	1516
BspPI	GGATC	4 cut(s)	62, 338, 610, 749
BsrDI	GCAATG	3 cut(s)	621, 795, 1371
BsrI	ACTGG	2 cut(s)	297, 691
BssECI	CCNNGG	7 cut(s)	503, 518, 899, 1285, 1409, 1425, 1567
BssMI	GATC	5 cut(s)	67, 343, 602, 754, 1202
BssSI	CACGAG	1 cut(s)	1479
BssT1I	CCWWGG	3 cut(s)	899, 1409, 1425
Bst2BI	CACGAG	1 cut(s)	1479
Bst2UI	CCWGG	3 cut(s)	504, 519, 1286
Bst4CI	ACNGT	2 cut(s)	1397, 1576
Bst6I	CTCTTC	2 cut(s)	93, 632
BstC8I	GCNNGC	6 cut(s)	240, 663, 1056, 1222, 1226, 1441
BstDEI	CTNAG	4 cut(s)	23, 129, 302, 718
BstDSI	CCRYGG	2 cut(s)	899, 1567
BstENI	CCTNNNNNAGG	2 cut(s)	776, 1289
BstF5I	GGATG	3 cut(s)	679, 787, 1389
BstFNI	CGCG	3 cut(s)	62, 1224, 1569
BstH2I	RGCGCY	2 cut(s)	190, 1072
BstHHI	GCGC	2 cut(s)	189, 1071
BstKTI	GATC	5 cut(s)	70, 346, 605, 757, 1205
BstMAI	GTCTC	5 cut(s)	42, 210, 305, 708, 1465
BstMBI	GATC	5 cut(s)	67, 343, 602, 754, 1202
BstMWI	GCNNNNNNNGC	4 cut(s)	184, 830, 1382, 1540
BstNI	CCWGG	3 cut(s)	504, 519, 1286
BstNSI	RCATGY	1 cut(s)	1616
BstPAI	GACNNNNGTC	1 cut(s)	297
BstSCI	CCNGG	4 cut(s)	502, 517, 1284, 1538
BstSFI	CTRYAG	1 cut(s)	924
BstSLI	GKGCMC	1 cut(s)	1417
BstUI	CGCG	3 cut(s)	62, 1224, 1569
BstV1I	GCAGC	4 cut(s)	645, 817, 1045, 1392
BstX2I	RGATCY	1 cut(s)	343
BstYI	RGATCY	1 cut(s)	343
BsuRI	GGCC	7 cut(s)	228, 242, 1175, 1284, 1385, 1534, 1543
BtgI	CCRYGG	2 cut(s)	899, 1567
BtgZI	GCGATG	1 cut(s)	839
BtsCI	GGATG	3 cut(s)	679, 787, 1389
BtsI	GCAGTG	2 cut(s)	46, 929
BtsIMutI	CAGTG	2 cut(s)	46, 929
BtuMI	TCGCGA	2 cut(s)	62, 1224
BveI	ACCTGC	1 cut(s)	1516
Cac8I	GCNNGC	6 cut(s)	240, 663, 1056, 1222, 1226, 1441
CaiI	CAGNNNCTG	2 cut(s)	347, 1508
CfoI	GCGC	2 cut(s)	189, 1071
Cfr13I	GGNCC	4 cut(s)	813, 1173, 1532, 1646
Cfr42I	CCGCGG	1 cut(s)	1570
Csp6I	GTAC	1 cut(s)	1572
CviQI	GTAC	1 cut(s)	1572
DdeI	CTNAG	4 cut(s)	23, 129, 302, 718
DpnI	GATC	5 cut(s)	69, 345, 604, 756, 1204
DpnII	GATC	5 cut(s)	67, 343, 602, 754, 1202
DraIII	CACNNNGTG	1 cut(s)	935
DrdI	GACNNNNNNGTC	1 cut(s)	56
DseDI	GACNNNNNNGTC	1 cut(s)	56
EaeI	YGGCCR	2 cut(s)	240, 1383
Eam1104I	CTCTTC	2 cut(s)	93, 632
EarI	CTCTTC	2 cut(s)	93, 632
Ecl136II	GAGCTC	1 cut(s)	597
Eco130I	CCWWGG	3 cut(s)	899, 1409, 1425
Eco147I	AGGCCT	1 cut(s)	1284
Eco24I	GRGCYC	2 cut(s)	599, 1480
Eco47I	GGWCC	2 cut(s)	813, 1646
Eco47III	AGCGCT	2 cut(s)	188, 1070
Eco53kI	GAGCTC	1 cut(s)	597
Eco88I	CYCGRG	2 cut(s)	598, 650
EcoICRI	GAGCTC	1 cut(s)	597
EcoNI	CCTNNNNNAGG	2 cut(s)	776, 1289
EcoO109I	RGGNCCY	1 cut(s)	1532
EcoRI	GAATTC	2 cut(s)	133, 1046
EcoRII	CCWGG	3 cut(s)	502, 517, 1284
EcoT14I	CCWWGG	3 cut(s)	899, 1409, 1425
EcoT38I	GRGCYC	2 cut(s)	599, 1480
ErhI	CCWWGG	3 cut(s)	899, 1409, 1425
Esp3I	CGTCTC	1 cut(s)	1465
FalI	AAGNNNNNCTT	2 cut(s)	1003, 1035
FaqI	GGGAC	1 cut(s)	1302
FauI	CCCGC	1 cut(s)	79
FblI	GTMKAC	1 cut(s)	552
Fnu4HI	GCNGC	4 cut(s)	659, 831, 1059, 1406
FokI	GGATG	3 cut(s)	686, 794, 1376
FriOI	GRGCYC	2 cut(s)	599, 1480
Fsp4HI	GCNGC	4 cut(s)	659, 831, 1059, 1406
FspBI	CTAG	4 cut(s)	1118, 1272, 1280, 1410
GlaI	GCGC	2 cut(s)	188, 1070
GluI	GCNGC	4 cut(s)	659, 831, 1059, 1406
GsaI	CCCAGC	1 cut(s)	1174
GsuI	CTGGAG	2 cut(s)	1059, 1489
HaeII	RGCGCY	2 cut(s)	190, 1072
HaeIII	GGCC	7 cut(s)	228, 242, 1175, 1284, 1385, 1534, 1543
HapII	CCGG	1 cut(s)	1540
HhaI	GCGC	2 cut(s)	189, 1071
Hin6I	GCGC	2 cut(s)	187, 1069
HinP1I	GCGC	2 cut(s)	187, 1069
HincII	GTYRAC	1 cut(s)	385
HindII	GTYRAC	1 cut(s)	385
HindIII	AAGCTT	2 cut(s)	395, 1125
HinfI	GANTC	7 cut(s)	27, 50, 80, 359, 716, 722, 1295
HpaII	CCGG	1 cut(s)	1540
HphI	GGTGA	1 cut(s)	1646
Hpy166II	GTNNAC	6 cut(s)	160, 385, 553, 1199, 1637, 1649
Hpy188I	TCNGA	9 cut(s)	24, 79, 139, 378, 721, 727, 1312, 1456, 1675
Hpy8I	GTNNAC	6 cut(s)	160, 385, 553, 1199, 1637, 1649
HpyAV	CCTTC	7 cut(s)	239, 577, 782, 1545, 1554, 1637, 1663
HpyCH4III	ACNGT	2 cut(s)	1397, 1576
HpyCH4IV	ACGT	1 cut(s)	381
HpyF10VI	GCNNNNNNNGC	4 cut(s)	184, 830, 1382, 1540
HpyF3I	CTNAG	4 cut(s)	23, 129, 302, 718
HpySE526I	ACGT	1 cut(s)	381
HspAI	GCGC	2 cut(s)	187, 1069
KspI	CCGCGG	1 cut(s)	1570
Kzo9I	GATC	5 cut(s)	67, 343, 602, 754, 1202
LmnI	GCTCC	1 cut(s)	652
Lsp1109I	GCAGC	4 cut(s)	645, 817, 1045, 1392
LweI	GCATC	5 cut(s)	28, 277, 635, 645, 1604
MaeI	CTAG	4 cut(s)	1118, 1272, 1280, 1410
MaeII	ACGT	1 cut(s)	381
MaeIII	GTNAC	1 cut(s)	1363
MalI	GATC	5 cut(s)	69, 345, 604, 756, 1204
MboI	GATC	5 cut(s)	67, 343, 602, 754, 1202
MboII	GAAGA	7 cut(s)	80, 115, 239, 521, 649, 1108, 1673
MfeI	CAATTG	2 cut(s)	741, 1163
MflI	RGATCY	1 cut(s)	343
MhlI	GDGCHC	3 cut(s)	599, 1417, 1480
MlsI	TGGCCA	2 cut(s)	242, 1385
MluNI	TGGCCA	2 cut(s)	242, 1385
MlyI	GAGTC	3 cut(s)	44, 74, 710
MmeI	TCCRAC	2 cut(s)	102, 592
Mox20I	TGGCCA	2 cut(s)	242, 1385
MroXI	GAANNNNTTC	2 cut(s)	31, 1015
MscI	TGGCCA	2 cut(s)	242, 1385
MseI	TTAA	5 cut(s)	171, 401, 446, 852, 1496
MslI	CAYNNNNRTG	1 cut(s)	1212
Msp20I	TGGCCA	2 cut(s)	242, 1385
MspA1I	CMGCKG	1 cut(s)	1569
MspI	CCGG	1 cut(s)	1540
MspR9I	CCNGG	4 cut(s)	504, 519, 1286, 1540
MunI	CAATTG	2 cut(s)	741, 1163
MvaI	CCWGG	3 cut(s)	504, 519, 1286
MvnI	CGCG	3 cut(s)	62, 1224, 1569
MwoI	GCNNNNNNNGC	4 cut(s)	184, 830, 1382, 1540
NciI	CCSGG	1 cut(s)	1540
NcoI	CCATGG	1 cut(s)	899
NdeII	GATC	5 cut(s)	67, 343, 602, 754, 1202
NlaIV	GGNNCC	2 cut(s)	648, 1533
NruI	TCGCGA	2 cut(s)	62, 1224
NspI	RCATGY	1 cut(s)	1616
PceI	AGGCCT	1 cut(s)	1284
PciI	ACATGT	1 cut(s)	1612
PdmI	GAANNNNTTC	2 cut(s)	31, 1015
PfeI	GAWTC	4 cut(s)	27, 359, 722, 1295
PkrI	GCNGC	4 cut(s)	660, 832, 1060, 1407
PleI	GAGTC	3 cut(s)	44, 74, 710
PpsI	GAGTC	3 cut(s)	44, 74, 710
PscI	ACATGT	1 cut(s)	1612
PshAI	GACNNNNGTC	1 cut(s)	297
PshBI	ATTAAT	1 cut(s)	852
Psp124BI	GAGCTC	1 cut(s)	599
Psp6I	CCWGG	3 cut(s)	502, 517, 1284
PspFI	CCCAGC	1 cut(s)	1170
PspGI	CCWGG	3 cut(s)	502, 517, 1284
PspN4I	GGNNCC	2 cut(s)	648, 1533
PspPI	GGNCC	4 cut(s)	813, 1173, 1532, 1646
PstNI	CAGNNNCTG	2 cut(s)	347, 1508
PsuI	RGATCY	1 cut(s)	343
RruI	TCGCGA	2 cut(s)	62, 1224
RsaI	GTAC	1 cut(s)	1573
RsaNI	GTAC	1 cut(s)	1572
RseI	CAYNNNNRTG	1 cut(s)	1212
SacI	GAGCTC	1 cut(s)	599
SacII	CCGCGG	1 cut(s)	1570
SaqAI	TTAA	5 cut(s)	171, 401, 446, 852, 1496
SatI	GCNGC	4 cut(s)	659, 831, 1059, 1406
Sau3AI	GATC	5 cut(s)	67, 343, 602, 754, 1202
Sau96I	GGNCC	4 cut(s)	813, 1173, 1532, 1646
SchI	GAGTC	3 cut(s)	44, 74, 710
ScrFI	CCNGG	4 cut(s)	504, 519, 1286, 1540
SduI	GDGCHC	3 cut(s)	599, 1417, 1480
SfaNI	GCATC	5 cut(s)	28, 277, 635, 645, 1604
SfcI	CTRYAG	1 cut(s)	924
SfiI	GGCCNNNNNGGCC	1 cut(s)	1540
Sfr303I	CCGCGG	1 cut(s)	1570
SgrBI	CCGCGG	1 cut(s)	1570
SinI	GGWCC	2 cut(s)	813, 1646
SmiMI	CAYNNNNRTG	1 cut(s)	1212
SseBI	AGGCCT	1 cut(s)	1284
SsiI	CCGC	3 cut(s)	72, 1567, 1569
SspI	AATATT	1 cut(s)	999
SspMI	CTAG	4 cut(s)	1118, 1272, 1280, 1410
SstI	GAGCTC	1 cut(s)	599
StuI	AGGCCT	1 cut(s)	1284
StyD4I	CCNGG	4 cut(s)	502, 517, 1284, 1538
StyI	CCWWGG	3 cut(s)	899, 1409, 1425
TaaI	ACNGT	2 cut(s)	1397, 1576
TaiI	ACGT	1 cut(s)	384
TaqI	TCGA	2 cut(s)	214, 1447
TfiI	GAWTC	4 cut(s)	27, 359, 722, 1295
Tru1I	TTAA	5 cut(s)	171, 401, 446, 852, 1496
Tru9I	TTAA	5 cut(s)	171, 401, 446, 852, 1496
TscAI	CASTG	2 cut(s)	46, 936
TseI	GCWGC	4 cut(s)	658, 830, 1058, 1405
TspDTI	ATGAA	9 cut(s)	129, 309, 506, 690, 798, 1039, 1230, 1257, 1272
TspGWI	ACGGA	2 cut(s)	88, 305
TspRI	CASTG	2 cut(s)	46, 936
VpaK11BI	GGWCC	2 cut(s)	813, 1646
VspI	ATTAAT	1 cut(s)	852
XagI	CCTNNNNNAGG	2 cut(s)	776, 1289
XapI	RAATTY	3 cut(s)	133, 371, 1046
XceI	RCATGY	1 cut(s)	1616
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1378
XmaJI	CCTAGG	1 cut(s)	1409
XmiI	GTMKAC	1 cut(s)	552
XmnI	GAANNNNTTC	2 cut(s)	31, 1015
XspI	CTAG	4 cut(s)	1118, 1272, 1280, 1410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.