Rroxscaffold_5G00344310

AP-5 complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0006853
Physical Location & Seq
Reverse (-)
14313807 .. 14322202
8396 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00344310.1

Sequence Viewer

Length: 1506 bp
ATGCATGCAATTATAGTGAAGAAGCTATATGAATTGTGTAAAGCTGAGAATTCAGAGGATTTGGTGGCCAGGGTTTATCCGCAGATTAACAAGCTCTTTCAACGCTCCGTCGCTTCTCTCTCTGAATCTCGAACCACCTCTTATGGCCTCCTCTTGCTGGCCATTCTTCAATTTTGCCTTGATTTTGGAGACTTGGTTCTGCATGATGCTGATCCCAGTCTCAGGACATTTTTCCGTTCATGCTTGAGCCGTGAGTTTGCAGATCCAGTTGTTGCGGAAGCAACCCTTGACTTTCTGAATGTAAACAAGAAAAAGCTCTTAACTTCTTTCCCTACCTTACTGCCTCAGATTAGAAAAATTATTTTTGAAGGTGTTTCCAGGATTGATGTCCCCAGGGTCATTTCTTCCGTTATTTCCATCTTTAGTGGACTTGCCAAATTGATGATTTTTGTTTCAACTATGGTAGTTTTGGTGGTGGCATTGGAGAAAGTAGAAAGGAGCTCAGGATCATTAGTTGGAAACAGCATAGCTTCAATGCAGAAGAGTTCAGCTCCTGAGATGCTGCTGGCGCTTATGGACGAAGCTTATACTGGTTCAACAATAGGAGATGGAGGGGGAGACTCTGAGTCAGAGGACACCAATGCAATAGATGTTGCTGACCCTCTGTTCCTCGACCTTTTAAAAGATGAAAATGATGGCATCGCTGAACGCCACTTGACTTCTCCTGGGATAGTCGCAGCCTTACAGGCTGCAATAAATACCCCTCGATCTGATAGACTGAAATATATACTTCAGATGGCACCACGGCTTCTTGATTTGTACTTTTCTATAGCATTGCGTGATGTCAATAACTCTTTGATCTGCGCATTGATTCCCCTACTCATGTCTAGGACTGGTACAATATTTCCTGACAAAGTTTATTCTTATGAGGTCCGTAAGAGGCTTTTAGAATTCATGCTTGCCGCCTTTCAGCGCTCTCCAGATTTTATTGCGCTTTTGAAGAAGCCTATAATGGACAGGCTTGCAGAAGCTTATAGTGGACAAGCTTATGACAGCCCTGAAAAGGTACGGATAAAACAGCCTTTGCTCATGTCTGGCCTACGGCAAGAGTCTGCCTTGAGCTCAGATAACCAAACTTCAAGAAAGTCATCACAGTCAAGGCTTCTATGTTTTGTTATTACAGCCATTGCAAAGCTGGCAACCAACCATCGTGAACTGCTGCCCAGGGCACGCTTTTCCTTGAGCAAGGTAGTTAGGTCTCGGATATCGGATGTGAGGGTCTGGAGACGGGCTTGTGATTATTTGGGTTTAATAAATGAACCCGCAATTTCCTCGTCTGTTTTGGGGCCTTCACGACCTTCACATGGACAAATGCAGAAGCCAGGTACTGTAAAGTGGAGCGAGGGCACAAAGATGATTGCACATGTTCCATTCTATATTCTTGGTGAACAAGAAGGTCAGGATCCTTCATCAAAACCTTTCAGTTTACCCAATCTTCTAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPG48

Protein Analysis

501

Amino Acids

55.53

Weight (kDa)

8.95

Isoelectric Point (pI)

46.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPG48 PF14764 6 - 116 4.2e-29 AP-5 complex subunit, vesicle trafficking
TPR_AP5Z1_C PF25154 254 - 341 2.1e-08 AP-5 complex subunit zeta-1, C-terminal TPR repeats
TPR_AP5Z1_C PF25154 375 - 453 4.9e-08 AP-5 complex subunit zeta-1, C-terminal TPR repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 799
AciI CCGC 4 cut(s) 80, 275, 963, 1323
AclWI GGATC 5 cut(s) 206, 257, 514, 1457, 1470
AcoI YGGCCR 2 cut(s) 66, 159
AcsI RAATTY 2 cut(s) 49, 950
AcuI CTGAAG 1 cut(s) 776
AfaI GTAC 4 cut(s) 821, 898, 1068, 1387
AfeI AGCGCT 1 cut(s) 974
AfiI CCNNNNNNNGG 4 cut(s) 157, 222, 1063, 1364
AflIII ACRYGT 1 cut(s) 1423
AgsI TTSAA 8 cut(s) 101, 170, 368, 456, 534, 597, 1000, 1140
AhdI GACNNNNNGTC 1 cut(s) 625
AjnI CCWGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
AloI GAACNNNNNNTCC 2 cut(s) 180, 212
Alw21I GWGCWC 2 cut(s) 503, 1124
Alw26I GTCTC 5 cut(s) 183, 224, 612, 1263, 1279
AlwI GGATC 5 cut(s) 206, 257, 514, 1457, 1470
AlwNI CAGNNNCTG 2 cut(s) 554, 1388
Aor51HI AGCGCT 1 cut(s) 974
AoxI GGCC 5 cut(s) 66, 145, 159, 1096, 1346
ApeKI GCWGC 4 cut(s) 562, 737, 749, 1219
ApoI RAATTY 2 cut(s) 49, 950
AspLEI GCGC 4 cut(s) 571, 866, 975, 994
AspS9I GGNCC 2 cut(s) 931, 1346
AsuHPI GGTGA 1 cut(s) 1457
AvaII GGWCC 1 cut(s) 931
BaeGI GKGCMC 2 cut(s) 1231, 1409
BalI TGGCCA 2 cut(s) 68, 161
BamHI GGATCC 1 cut(s) 1462
BanI GGYRCC 1 cut(s) 799
BanII GRGCYC 2 cut(s) 503, 1124
Bbv12I GWGCWC 2 cut(s) 503, 1124
BbvI GCAGC 4 cut(s) 549, 736, 749, 1206
BccI CCATC 5 cut(s) 425, 602, 689, 790, 1215
BceAI ACGGC 3 cut(s) 234, 821, 1118
BcgI CGANNNNNNTGC 2 cut(s) 1314, 1348
BciT130I CCWGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
BcoDI GTCTC 5 cut(s) 183, 224, 612, 1263, 1279
BfaI CTAG 1 cut(s) 888
BfmI CTRYAG 1 cut(s) 828
BfoI RGCGCY 2 cut(s) 572, 976
BglI GCCNNNNNGGC 1 cut(s) 746
BisI GCNGC 5 cut(s) 563, 738, 750, 963, 1220
BlsI GCNGC 5 cut(s) 564, 739, 751, 964, 1221
Bme1390I CCNGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
Bme18I GGWCC 1 cut(s) 931
BmeRI GACNNNNNGTC 1 cut(s) 625
BmgT120I GGNCC 2 cut(s) 931, 1346
BmiI GGNNCC 3 cut(s) 801, 1347, 1464
BmrFI CCNGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
BmrI ACTGGG 1 cut(s) 210
BmsI GCATC 3 cut(s) 196, 549, 708
BmuI ACTGGG 1 cut(s) 210
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpmI CTGGAG 2 cut(s) 963, 1303
Bpu10I CCTNAGC 1 cut(s) 502
BpuEI CTTGAG 3 cut(s) 265, 1138, 1261
BsaBI GATNNNNATC 1 cut(s) 210
BsaI GGTCTC 1 cut(s) 1263
BsaJI CCNNGG 7 cut(s) 69, 392, 393, 725, 803, 1223, 1224
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 4 cut(s) 157, 222, 1063, 1364
Bse1I ACTGG 4 cut(s) 216, 266, 595, 898
Bse3DI GCAATG 2 cut(s) 833, 1185
Bse8I GATNNNNATC 1 cut(s) 210
BseBI CCWGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
BseDI CCNNGG 7 cut(s) 69, 392, 393, 725, 803, 1223, 1224
BseGI GGATG 1 cut(s) 1276
BseJI GATNNNNATC 1 cut(s) 210
BseLI CCNNNNNNNGG 4 cut(s) 157, 222, 1063, 1364
BseMI GCAATG 2 cut(s) 833, 1185
BseMII CTCAG 7 cut(s) 36, 235, 359, 516, 546, 615, 1137
BseNI ACTGG 4 cut(s) 216, 266, 595, 898
BseRI GAGGAG 1 cut(s) 140
BseSI GKGCMC 2 cut(s) 1231, 1409
BseXI GCAGC 4 cut(s) 549, 736, 749, 1206
BshFI GGCC 5 cut(s) 68, 147, 161, 1098, 1348
BshNI GGYRCC 1 cut(s) 799
BsiHKAI GWGCWC 2 cut(s) 503, 1124
BslFI GGGAC 1 cut(s) 374
BslI CCNNNNNNNGG 4 cut(s) 157, 222, 1063, 1364
BsmAI GTCTC 5 cut(s) 183, 224, 612, 1263, 1279
BsmBI CGTCTC 1 cut(s) 1279
BsmFI GGGAC 1 cut(s) 374
BsnI GGCC 5 cut(s) 68, 147, 161, 1098, 1348
Bso31I GGTCTC 1 cut(s) 1263
Bsp1286I GDGCHC 4 cut(s) 503, 1124, 1231, 1409
Bsp143I GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
BspACI CCGC 4 cut(s) 80, 275, 963, 1323
BspANI GGCC 5 cut(s) 68, 147, 161, 1098, 1348
BspCNI CTCAG 7 cut(s) 37, 234, 358, 515, 547, 616, 1136
BspLI GGNNCC 3 cut(s) 801, 1347, 1464
BspPI GGATC 5 cut(s) 206, 257, 514, 1457, 1470
BspT107I GGYRCC 1 cut(s) 799
BspTNI GGTCTC 1 cut(s) 1263
BsrDI GCAATG 2 cut(s) 833, 1185
BsrI ACTGG 4 cut(s) 216, 266, 595, 898
BssECI CCNNGG 7 cut(s) 69, 392, 393, 725, 803, 1223, 1224
BssMI GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
Bst2UI CCWGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
Bst4CI ACNGT 2 cut(s) 1155, 1390
Bst6I CTCTTC 1 cut(s) 536
BstC8I GCNNGC 7 cut(s) 6, 159, 567, 960, 1023, 1197, 1231
BstDEI CTNAG 7 cut(s) 45, 221, 345, 502, 555, 624, 1123
BstDSI CCRYGG 1 cut(s) 803
BstF5I GGATG 1 cut(s) 1276
BstH2I RGCGCY 2 cut(s) 572, 976
BstHHI GCGC 4 cut(s) 571, 866, 975, 994
BstKTI GATC 6 cut(s) 214, 265, 509, 770, 861, 1465
BstMAI GTCTC 5 cut(s) 183, 224, 612, 1263, 1279
BstMBI GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
BstMWI GCNNNNNNNGC 3 cut(s) 568, 746, 1196
BstNI CCWGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
BstNSI RCATGY 2 cut(s) 8, 1427
BstSCI CCNGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
BstSFI CTRYAG 1 cut(s) 828
BstSLI GKGCMC 2 cut(s) 1231, 1409
BstV1I GCAGC 4 cut(s) 549, 736, 749, 1206
BstX2I RGATCY 2 cut(s) 262, 1462
BstYI RGATCY 2 cut(s) 262, 1462
BsuRI GGCC 5 cut(s) 68, 147, 161, 1098, 1348
BtgI CCRYGG 1 cut(s) 803
BtgZI GCGATG 1 cut(s) 685
BtsCI GGATG 1 cut(s) 1276
Cac8I GCNNGC 7 cut(s) 6, 159, 567, 960, 1023, 1197, 1231
CaiI CAGNNNCTG 2 cut(s) 554, 1388
CfoI GCGC 4 cut(s) 571, 866, 975, 994
Cfr13I GGNCC 2 cut(s) 931, 1346
Csp6I GTAC 4 cut(s) 820, 897, 1067, 1386
CviAII CATG 8 cut(s) 5, 203, 240, 883, 955, 1090, 1364, 1424
CviQI GTAC 4 cut(s) 820, 897, 1067, 1386
DdeI CTNAG 7 cut(s) 45, 221, 345, 502, 555, 624, 1123
DpnI GATC 6 cut(s) 213, 264, 508, 769, 860, 1464
DpnII GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
DraI TTTAAA 1 cut(s) 681
DriI GACNNNNNGTC 1 cut(s) 625
EaeI YGGCCR 2 cut(s) 66, 159
Eam1104I CTCTTC 1 cut(s) 536
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 1 cut(s) 536
Ecl136II GAGCTC 2 cut(s) 501, 1122
Eco24I GRGCYC 2 cut(s) 503, 1124
Eco31I GGTCTC 1 cut(s) 1263
Eco32I GATATC 1 cut(s) 1266
Eco47I GGWCC 1 cut(s) 931
Eco47III AGCGCT 1 cut(s) 974
Eco53kI GAGCTC 2 cut(s) 501, 1122
Eco57I CTGAAG 1 cut(s) 776
EcoICRI GAGCTC 2 cut(s) 501, 1122
EcoO109I RGGNCCY 1 cut(s) 1346
EcoRI GAATTC 2 cut(s) 49, 950
EcoRII CCWGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
EcoRV GATATC 1 cut(s) 1266
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 503, 1124
Esp3I CGTCTC 1 cut(s) 1279
FaeI CATG 8 cut(s) 8, 206, 243, 886, 958, 1093, 1367, 1427
FalI AAGNNNNNCTT 2 cut(s) 270, 302
FaqI GGGAC 1 cut(s) 374
FatI CATG 8 cut(s) 4, 202, 239, 882, 954, 1089, 1363, 1423
FauI CCCGC 1 cut(s) 1330
Fnu4HI GCNGC 5 cut(s) 563, 738, 750, 963, 1220
FokI GGATG 1 cut(s) 1283
FriOI GRGCYC 2 cut(s) 503, 1124
Fsp4HI GCNGC 5 cut(s) 563, 738, 750, 963, 1220
FspBI CTAG 1 cut(s) 888
FspI TGCGCA 1 cut(s) 865
GlaI GCGC 4 cut(s) 570, 865, 974, 993
GluI GCNGC 5 cut(s) 563, 738, 750, 963, 1220
GsuI CTGGAG 2 cut(s) 963, 1303
HaeII RGCGCY 2 cut(s) 572, 976
HaeIII GGCC 5 cut(s) 68, 147, 161, 1098, 1348
HhaI GCGC 4 cut(s) 571, 866, 975, 994
Hin1II CATG 8 cut(s) 8, 206, 243, 886, 958, 1093, 1367, 1427
Hin6I GCGC 4 cut(s) 569, 864, 973, 992
HinP1I GCGC 4 cut(s) 569, 864, 973, 992
HindIII AAGCTT 3 cut(s) 582, 1029, 1044
HinfI GANTC 5 cut(s) 125, 620, 626, 871, 1109
HphI GGTGA 1 cut(s) 1457
Hpy166II GTNNAC 6 cut(s) 304, 428, 1040, 1214, 1448, 1487
Hpy8I GTNNAC 6 cut(s) 304, 428, 1040, 1214, 1448, 1487
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 5 cut(s) 362, 1359, 1368, 1448, 1476
HpyCH4III ACNGT 2 cut(s) 1155, 1390
HpyF10VI GCNNNNNNNGC 3 cut(s) 568, 746, 1196
HpyF3I CTNAG 7 cut(s) 45, 221, 345, 502, 555, 624, 1123
Hsp92II CATG 8 cut(s) 8, 206, 243, 886, 958, 1093, 1367, 1427
HspAI GCGC 4 cut(s) 569, 864, 973, 992
Kzo9I GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
LmnI GCTCC 4 cut(s) 110, 498, 556, 1398
Lsp1109I GCAGC 4 cut(s) 549, 736, 749, 1206
LweI GCATC 3 cut(s) 196, 549, 708
MaeI CTAG 1 cut(s) 888
MalI GATC 6 cut(s) 213, 264, 508, 769, 860, 1464
MboI GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
MboII GAAGA 6 cut(s) 31, 158, 396, 553, 1012, 1487
MflI RGATCY 2 cut(s) 262, 1462
MhlI GDGCHC 4 cut(s) 503, 1124, 1231, 1409
MlsI TGGCCA 2 cut(s) 68, 161
MluCI AATT 8 cut(s) 9, 32, 49, 170, 357, 437, 950, 1326
MluNI TGGCCA 2 cut(s) 68, 161
MlyI GAGTC 3 cut(s) 614, 635, 1118
MmeI TCCRAC 1 cut(s) 496
Mox20I TGGCCA 2 cut(s) 68, 161
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 2 cut(s) 68, 161
MseI TTAA 4 cut(s) 87, 320, 680, 1310
MslI CAYNNNNRTG 1 cut(s) 1412
Msp20I TGGCCA 2 cut(s) 68, 161
MspR9I CCNGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
MvaI CCWGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
MwoI GCNNNNNNNGC 3 cut(s) 568, 746, 1196
NdeII GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
NlaIII CATG 8 cut(s) 8, 206, 243, 886, 958, 1093, 1367, 1427
NlaIV GGNNCC 3 cut(s) 801, 1347, 1464
NsbI TGCGCA 1 cut(s) 865
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 1427
PaeI GCATGC 1 cut(s) 8
PasI CCCWGGG 2 cut(s) 393, 1224
PciI ACATGT 1 cut(s) 1423
PfeI GAWTC 2 cut(s) 125, 871
PfoI TCCNGGA 1 cut(s) 377
PkrI GCNGC 5 cut(s) 564, 739, 751, 964, 1221
PleI GAGTC 3 cut(s) 614, 634, 1117
PpsI GAGTC 3 cut(s) 614, 634, 1117
PscI ACATGT 1 cut(s) 1423
Psp124BI GAGCTC 2 cut(s) 503, 1124
Psp6I CCWGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
PspGI CCWGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
PspN4I GGNNCC 3 cut(s) 801, 1347, 1464
PspPI GGNCC 2 cut(s) 931, 1346
PstNI CAGNNNCTG 2 cut(s) 554, 1388
PsuI RGATCY 2 cut(s) 262, 1462
RsaI GTAC 4 cut(s) 821, 898, 1068, 1387
RsaNI GTAC 4 cut(s) 820, 897, 1067, 1386
RseI CAYNNNNRTG 1 cut(s) 1412
SacI GAGCTC 2 cut(s) 503, 1124
SaqAI TTAA 4 cut(s) 87, 320, 680, 1310
SatI GCNGC 5 cut(s) 563, 738, 750, 963, 1220
Sau3AI GATC 6 cut(s) 211, 262, 506, 767, 858, 1462
Sau96I GGNCC 2 cut(s) 931, 1346
SchI GAGTC 3 cut(s) 614, 635, 1118
ScrFI CCNGG 6 cut(s) 70, 379, 394, 726, 1225, 1383
SduI GDGCHC 4 cut(s) 503, 1124, 1231, 1409
SfaNI GCATC 3 cut(s) 196, 549, 708
SfcI CTRYAG 1 cut(s) 828
SinI GGWCC 1 cut(s) 931
SmiMI CAYNNNNRTG 1 cut(s) 1412
SmlI CTYRAG 3 cut(s) 244, 1117, 1240
SmoI CTYRAG 3 cut(s) 244, 1117, 1240
SphI GCATGC 1 cut(s) 8
Sse9I AATT 8 cut(s) 9, 32, 49, 170, 357, 437, 950, 1326
SsiI CCGC 4 cut(s) 80, 275, 963, 1323
SspI AATATT 1 cut(s) 903
SspMI CTAG 1 cut(s) 888
SstI GAGCTC 2 cut(s) 503, 1124
StyD4I CCNGG 6 cut(s) 68, 377, 392, 724, 1223, 1381
TaaI ACNGT 2 cut(s) 1155, 1390
TaqI TCGA 3 cut(s) 130, 672, 766
TasI AATT 8 cut(s) 9, 32, 49, 170, 357, 437, 950, 1326
TatI WGTACW 1 cut(s) 819
TauI GCSGC 1 cut(s) 965
TfiI GAWTC 2 cut(s) 125, 871
Tru1I TTAA 4 cut(s) 87, 320, 680, 1310
Tru9I TTAA 4 cut(s) 87, 320, 680, 1310
TseI GCWGC 4 cut(s) 562, 737, 749, 1219
TspDTI ATGAA 6 cut(s) 45, 228, 702, 943, 1332, 1458
TspGWI ACGGA 5 cut(s) 97, 224, 397, 923, 1084
VpaK11BI GGWCC 1 cut(s) 931
XapI RAATTY 2 cut(s) 49, 950
XceI RCATGY 2 cut(s) 8, 1427
XcmI CCANNNNNNNNNTGG 1 cut(s) 1192
XspI CTAG 1 cut(s) 888
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.