Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0024336

Physical Location & Seq

Genomic Coordinates Reverse (-)

30152900 .. 30154673

Gene Structure 1774 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1217300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1032 bp

ATGGGGAAAAATCCTTGTTGTGAGACGAATGGATTGAAAAAGGGTCCATGGAGTCCGGAGGAAGATCATAAACTCGTTCATTACATTCAGAAACACGGGCATGGGAGATGGCGAACCCTCCCCAAGAATGCAGGACTGAAAAGGTGTGGAAAGAGTTGTCGCCTTCGGTGGGCAAACTATTTAAGACCTGATATTAAGAGAGGGAGATTCTCAATGGAAGAAGAAGAGACCATAATACAGTTACACAGTGTTTTGGGAAACAAGTGGTCTACCATTGCTTCTCGCTTGCCAGGAAGAACAGATAATGAGATCAAGAACTATTGGAACACACACATCAGAAAGAGGCTATTAAAAATGGGGATTGATCCTGTCACTCATACTCCTCGTCTCGATCTTCTTGAACTCACCTCCCTTCTCTCATCAACCCTCTATAACTCATCGTCAGATAATTTGCAGCTTAATATTCCGAATCTGCTCAGTATTGGACCTATAATGGATCCAGAGCTCCTCAACTTAGCCGCTACTTTGTTAACAAGCCAACGCAGACCGCAATCATCGCATCCTGAATTTCCTTCAAAGAATCATGCTCAACAAAACCCACTTGCCCTTTTACAAAACAATGATCAAAACCAATTTCAATCAAACCAGCTCCCAAATGTTGCCCCATTTCTAAACCAACCCCTTATCACTAATTTTGGCCACCCTTGTACTACAAATGGAGCCGATCAGAGTAGTTTTGTTCCTATAACAACAAACCAGGTGGGCTCGTATGACTCTAACTACGAGTCAATCATTAGCCATAATATTCCAGGACAAGATTACCAGCCGGCGTGCATTTCTGATAACGTTAATAATCAAAGCTTTCAAAATATGTGTAGCTTTGGTTCGCTGCTATCAACACCTTCCTCAAGTCCGGCCACCACTACCTATGCCAATTGTTGCACAGAAGATGAGAGGGATAGTTTTTGCAGCAATATCTTAAATATGTATCATGACATTCCCAATGACTTAAACGTTACCCAACTTCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000988 GO:0000989 GO:0001067 GO:0001076 GO:0001134 GO:0001135 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0030154 GO:0031323 GO:0031326 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Myb_DNA-binding

Protein Analysis

344

Amino Acids

38.6

Weight (kDa)

7.17

Isoelectric Point (pI)

57.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Myb_DNA-binding	PF00249	14 - 61	1.2e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	17 - 77	1.9e-11	Myb-like DNA-binding domain
Myb_DNA-binding	PF00249	67 - 111	4.1e-17	Myb-like DNA-binding domain
Myb_DNA-bind_6	PF13921	70 - 114	2.5e-08	Myb-like DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0024336)

Species	Orthologous Gene IDs
malus_domestica	MD03G1217300.v1.1
prunus_persica	Prupe.4G192000_v2.0.a1
pyrus_communis	pycom03g16670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	269
AccIII	TCCGGA	1 cut(s)	55
AciI	CCGC	2 cut(s)	519, 548
AclI	AACGTT	2 cut(s)	846, 1014
AclWI	GGATC	3 cut(s)	359, 491, 504
AcoI	YGGCCR	2 cut(s)	697, 915
AcsI	RAATTY	1 cut(s)	566
AfaI	GTAC	1 cut(s)	709
AfiI	CCNNNNNNNGG	1 cut(s)	169
AgsI	TTSAA	5 cut(s)	37, 401, 576, 638, 866
AjnI	CCWGG	3 cut(s)	289, 756, 808
AluBI	AGCT	5 cut(s)	457, 505, 649, 861, 879
AluI	AGCT	5 cut(s)	457, 505, 649, 861, 879
Alw21I	GWGCWC	1 cut(s)	507
Alw26I	GTCTC	3 cut(s)	17, 221, 392
AlwI	GGATC	3 cut(s)	359, 491, 504
Aor13HI	TCCGGA	1 cut(s)	55
AoxI	GGCC	2 cut(s)	697, 915
ApeKI	GCWGC	3 cut(s)	454, 889, 969
ApoI	RAATTY	1 cut(s)	566
AspS9I	GGNCC	2 cut(s)	44, 485
AsuHPI	GGTGA	1 cut(s)	397
AvaII	GGWCC	2 cut(s)	44, 485
BalI	TGGCCA	1 cut(s)	699
BamHI	GGATCC	1 cut(s)	496
BanII	GRGCYC	2 cut(s)	507, 767
Bbv12I	GWGCWC	1 cut(s)	507
BbvI	GCAGC	3 cut(s)	466, 876, 981
BccI	CCATC	1 cut(s)	102
BciT130I	CCWGG	3 cut(s)	291, 758, 810
BclI	TGATCA	1 cut(s)	622
BcoDI	GTCTC	3 cut(s)	17, 221, 392
BisI	GCNGC	4 cut(s)	455, 519, 890, 970
BlsI	GCNGC	4 cut(s)	456, 520, 891, 971
Bme1390I	CCNGG	3 cut(s)	291, 758, 810
Bme18I	GGWCC	2 cut(s)	44, 485
BmgT120I	GGNCC	2 cut(s)	44, 485
BmiI	GGNNCC	3 cut(s)	45, 498, 721
BmrFI	CCNGG	3 cut(s)	291, 758, 810
BmsI	GCATC	1 cut(s)	568
BpuEI	CTTGAG	1 cut(s)	892
BsaI	GGTCTC	1 cut(s)	221
BsaJI	CCNNGG	1 cut(s)	47
BsaWI	WCCGGW	1 cut(s)	55
BsaXI	ACNNNNNCTCC	2 cut(s)	364, 394
Bsc4I	CCNNNNNNNGG	1 cut(s)	169
Bse118I	RCCGGY	1 cut(s)	826
Bse3DI	GCAATG	1 cut(s)	273
BseAI	TCCGGA	1 cut(s)	55
BseBI	CCWGG	3 cut(s)	291, 758, 810
BseDI	CCNNGG	1 cut(s)	47
BseGI	GGATG	1 cut(s)	559
BseLI	CCNNNNNNNGG	1 cut(s)	169
BseMI	GCAATG	1 cut(s)	273
BseMII	CTCAG	1 cut(s)	490
BseRI	GAGGAG	2 cut(s)	372, 497
BseXI	GCAGC	3 cut(s)	466, 876, 981
BshFI	GGCC	2 cut(s)	699, 917
BsiHKAI	GWGCWC	1 cut(s)	507
BsiSI	CCGG	3 cut(s)	56, 827, 914
BslI	CCNNNNNNNGG	1 cut(s)	169
BsmAI	GTCTC	3 cut(s)	17, 221, 392
BsmBI	CGTCTC	2 cut(s)	17, 392
BsmI	GAATGC	1 cut(s)	133
BsnI	GGCC	2 cut(s)	699, 917
Bso31I	GGTCTC	1 cut(s)	221
Bsp1286I	GDGCHC	2 cut(s)	507, 767
Bsp13I	TCCGGA	1 cut(s)	55
Bsp143I	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
Bsp19I	CCATGG	1 cut(s)	47
BspACI	CCGC	2 cut(s)	519, 548
BspANI	GGCC	2 cut(s)	699, 917
BspCNI	CTCAG	1 cut(s)	489
BspEI	TCCGGA	1 cut(s)	55
BspHI	TCATGA	1 cut(s)	991
BspLI	GGNNCC	3 cut(s)	45, 498, 721
BspPI	GGATC	3 cut(s)	359, 491, 504
BspTNI	GGTCTC	1 cut(s)	221
BsrDI	GCAATG	1 cut(s)	273
BsrFI	RCCGGY	1 cut(s)	826
BssAI	RCCGGY	1 cut(s)	826
BssECI	CCNNGG	1 cut(s)	47
BssMI	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
BssT1I	CCWWGG	1 cut(s)	47
Bst2UI	CCWGG	3 cut(s)	291, 758, 810
Bst4CI	ACNGT	2 cut(s)	240, 248
Bst6I	CTCTTC	1 cut(s)	219
BstC8I	GCNNGC	3 cut(s)	287, 828, 832
BstDEI	CTNAG	2 cut(s)	476, 514
BstDSI	CCRYGG	1 cut(s)	47
BstF5I	GGATG	1 cut(s)	559
BstKTI	GATC	7 cut(s)	67, 312, 367, 394, 499, 625, 727
BstMAI	GTCTC	3 cut(s)	17, 221, 392
BstMBI	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
BstMWI	GCNNNNNNNGC	1 cut(s)	556
BstNI	CCWGG	3 cut(s)	291, 758, 810
BstSCI	CCNGG	3 cut(s)	289, 756, 808
BstV1I	GCAGC	3 cut(s)	466, 876, 981
BstX2I	RGATCY	1 cut(s)	496
BstYI	RGATCY	1 cut(s)	496
BsuRI	GGCC	2 cut(s)	699, 917
BtgI	CCRYGG	1 cut(s)	47
BtgZI	GCGATG	1 cut(s)	540
BtsCI	GGATG	1 cut(s)	559
BtsIMutI	CAGTG	1 cut(s)	253
Cac8I	GCNNGC	3 cut(s)	287, 828, 832
CciI	TCATGA	1 cut(s)	991
Cfr10I	RCCGGY	1 cut(s)	826
Cfr13I	GGNCC	2 cut(s)	44, 485
CsiI	ACCWGGT	1 cut(s)	756
Csp6I	GTAC	1 cut(s)	708
CspCI	CAANNNNNGTGG	2 cut(s)	741, 776
CviAII	CATG	4 cut(s)	48, 101, 584, 992
CviQI	GTAC	1 cut(s)	708
DdeI	CTNAG	2 cut(s)	476, 514
DpnI	GATC	7 cut(s)	66, 311, 366, 393, 498, 624, 726
DpnII	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
EaeI	YGGCCR	2 cut(s)	697, 915
Eam1104I	CTCTTC	1 cut(s)	219
EarI	CTCTTC	1 cut(s)	219
Ecl136II	GAGCTC	1 cut(s)	505
Eco130I	CCWWGG	1 cut(s)	47
Eco24I	GRGCYC	2 cut(s)	507, 767
Eco31I	GGTCTC	1 cut(s)	221
Eco47I	GGWCC	2 cut(s)	44, 485
Eco53kI	GAGCTC	1 cut(s)	505
EcoICRI	GAGCTC	1 cut(s)	505
EcoRII	CCWGG	3 cut(s)	289, 756, 808
EcoT14I	CCWWGG	1 cut(s)	47
EcoT38I	GRGCYC	2 cut(s)	507, 767
ErhI	CCWWGG	1 cut(s)	47
Esp3I	CGTCTC	2 cut(s)	17, 392
FaeI	CATG	4 cut(s)	51, 104, 587, 995
FatI	CATG	4 cut(s)	47, 100, 583, 991
FbaI	TGATCA	1 cut(s)	622
FblI	GTMKAC	1 cut(s)	269
Fnu4HI	GCNGC	4 cut(s)	455, 519, 890, 970
FokI	GGATG	1 cut(s)	546
FriOI	GRGCYC	2 cut(s)	507, 767
Fsp4HI	GCNGC	4 cut(s)	455, 519, 890, 970
GluI	GCNGC	4 cut(s)	455, 519, 890, 970
HaeIII	GGCC	2 cut(s)	699, 917
HapII	CCGG	3 cut(s)	56, 827, 914
Hin1II	CATG	4 cut(s)	51, 104, 587, 995
HincII	GTYRAC	1 cut(s)	531
HindII	GTYRAC	1 cut(s)	531
HindIII	AAGCTT	1 cut(s)	859
HinfI	GANTC	6 cut(s)	52, 207, 469, 580, 773, 785
HpaI	GTTAAC	1 cut(s)	531
HpaII	CCGG	3 cut(s)	56, 827, 914
HphI	GGTGA	1 cut(s)	397
Hpy166II	GTNNAC	2 cut(s)	270, 531
Hpy188I	TCNGA	6 cut(s)	90, 338, 445, 468, 729, 841
Hpy188III	TCNNGA	7 cut(s)	56, 313, 389, 398, 500, 563, 992
Hpy8I	GTNNAC	2 cut(s)	270, 531
HpyAV	CCTTC	4 cut(s)	173, 422, 582, 912
HpyCH4III	ACNGT	2 cut(s)	240, 248
HpyCH4IV	ACGT	2 cut(s)	846, 1014
HpyCH4V	TGCA	5 cut(s)	131, 454, 834, 942, 969
HpyF10VI	GCNNNNNNNGC	1 cut(s)	556
HpyF3I	CTNAG	2 cut(s)	476, 514
HpySE526I	ACGT	2 cut(s)	846, 1014
Hsp92II	CATG	4 cut(s)	51, 104, 587, 995
Kpn2I	TCCGGA	1 cut(s)	55
KroI	GCCGGC	1 cut(s)	826
KroNI	GCCGGC	1 cut(s)	828
Ksp22I	TGATCA	1 cut(s)	622
KspAI	GTTAAC	1 cut(s)	531
Kzo9I	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
LmnI	GCTCC	3 cut(s)	510, 654, 719
Lsp1109I	GCAGC	3 cut(s)	466, 876, 981
LweI	GCATC	1 cut(s)	568
MabI	ACCWGGT	1 cut(s)	756
MaeII	ACGT	2 cut(s)	846, 1014
MaeIII	GTNAC	3 cut(s)	240, 370, 1015
MalI	GATC	7 cut(s)	66, 311, 366, 393, 498, 624, 726
MboI	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
MboII	GAAGA	7 cut(s)	74, 230, 233, 236, 306, 386, 959
MfeI	CAATTG	1 cut(s)	934
MflI	RGATCY	1 cut(s)	496
MhlI	GDGCHC	2 cut(s)	507, 767
MlsI	TGGCCA	1 cut(s)	699
MluCI	AATT	5 cut(s)	448, 566, 632, 691, 934
MluNI	TGGCCA	1 cut(s)	699
MlyI	GAGTC	3 cut(s)	61, 767, 794
Mox20I	TGGCCA	1 cut(s)	699
MroI	TCCGGA	1 cut(s)	55
MroNI	GCCGGC	1 cut(s)	826
MscI	TGGCCA	1 cut(s)	699
MseI	TTAA	8 cut(s)	182, 195, 350, 459, 530, 849, 980, 1010
MslI	CAYNNNNRTG	1 cut(s)	99
Msp20I	TGGCCA	1 cut(s)	699
MspI	CCGG	3 cut(s)	56, 827, 914
MspR9I	CCNGG	3 cut(s)	291, 758, 810
MunI	CAATTG	1 cut(s)	934
Mva1269I	GAATGC	1 cut(s)	133
MvaI	CCWGG	3 cut(s)	291, 758, 810
MwoI	GCNNNNNNNGC	1 cut(s)	556
NaeI	GCCGGC	1 cut(s)	828
NcoI	CCATGG	1 cut(s)	47
NdeII	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
NgoMIV	GCCGGC	1 cut(s)	826
NlaIII	CATG	4 cut(s)	51, 104, 587, 995
NlaIV	GGNNCC	3 cut(s)	45, 498, 721
NmuCI	GTSAC	1 cut(s)	370
PagI	TCATGA	1 cut(s)	991
PctI	GAATGC	1 cut(s)	133
PdiI	GCCGGC	1 cut(s)	828
PfeI	GAWTC	3 cut(s)	207, 469, 580
PfoI	TCCNGGA	1 cut(s)	808
PkrI	GCNGC	4 cut(s)	456, 520, 891, 971
PleI	GAGTC	3 cut(s)	60, 767, 793
PpsI	GAGTC	3 cut(s)	60, 767, 793
Psp124BI	GAGCTC	1 cut(s)	507
Psp1406I	AACGTT	2 cut(s)	846, 1014
Psp6I	CCWGG	3 cut(s)	289, 756, 808
PspGI	CCWGG	3 cut(s)	289, 756, 808
PspN4I	GGNNCC	3 cut(s)	45, 498, 721
PspPI	GGNCC	2 cut(s)	44, 485
PsrI	GAACNNNNNNTAC	2 cut(s)	868, 900
PsuI	RGATCY	1 cut(s)	496
RsaI	GTAC	1 cut(s)	709
RsaNI	GTAC	1 cut(s)	708
RseI	CAYNNNNRTG	1 cut(s)	99
SacI	GAGCTC	1 cut(s)	507
SaqAI	TTAA	8 cut(s)	182, 195, 350, 459, 530, 849, 980, 1010
SatI	GCNGC	4 cut(s)	455, 519, 890, 970
Sau3AI	GATC	7 cut(s)	64, 309, 364, 391, 496, 622, 724
Sau96I	GGNCC	2 cut(s)	44, 485
SchI	GAGTC	3 cut(s)	61, 767, 794
ScrFI	CCNGG	3 cut(s)	291, 758, 810
SduI	GDGCHC	2 cut(s)	507, 767
SexAI	ACCWGGT	1 cut(s)	756
SfaNI	GCATC	1 cut(s)	568
SinI	GGWCC	2 cut(s)	44, 485
SmiMI	CAYNNNNRTG	1 cut(s)	99
SmlI	CTYRAG	1 cut(s)	907
SmoI	CTYRAG	1 cut(s)	907
Sse9I	AATT	5 cut(s)	448, 566, 632, 691, 934
SsiI	CCGC	2 cut(s)	519, 548
SspI	AATATT	2 cut(s)	463, 805
SstI	GAGCTC	1 cut(s)	507
StyD4I	CCNGG	3 cut(s)	289, 756, 808
StyI	CCWWGG	1 cut(s)	47
TaaI	ACNGT	2 cut(s)	240, 248
TaiI	ACGT	2 cut(s)	849, 1017
TaqI	TCGA	1 cut(s)	390
TasI	AATT	5 cut(s)	448, 566, 632, 691, 934
TatI	WGTACW	1 cut(s)	707
TauI	GCSGC	1 cut(s)	521
TfiI	GAWTC	3 cut(s)	207, 469, 580
Tru1I	TTAA	8 cut(s)	182, 195, 350, 459, 530, 849, 980, 1010
Tru9I	TTAA	8 cut(s)	182, 195, 350, 459, 530, 849, 980, 1010
TscAI	CASTG	1 cut(s)	253
TseFI	GTSAC	1 cut(s)	370
TseI	GCWGC	3 cut(s)	454, 889, 969
Tsp45I	GTSAC	1 cut(s)	370
TspDTI	ATGAA	1 cut(s)	68
TspRI	CASTG	1 cut(s)	253
VpaK11BI	GGWCC	2 cut(s)	44, 485
XapI	RAATTY	1 cut(s)	566
XmiI	GTMKAC	1 cut(s)	269

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.