Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0028685

Physical Location & Seq

Genomic Coordinates Forward (+)

1147246 .. 1149364

Gene Structure 2119 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1010400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 312 bp

ATGTCTGACCCCTCTGGGATTATTACTCCTGATGAAGCCAAAGAGCAAAAACCCACCATTAATCTGAAGGTTAAGATTAACAAGGATGGAAGTGAAGTGTTTTTCAGGATTAAACCAACTACAAAGCTAAACAAAGTCATTGATGCATTGTGCACAAAACGTTCCCTGGATCGCAATTCCTTAGTGTTCATCTTCGATGGCGATCACATTAATGGCAAGAAGACTGCTGCGGAGCATCATATGGAAGATGGTGATGAAATCGATGTCTTTCTTCTCACTGATGGAGGTTCACTGACATTCAAAAGTTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016925 GO:0018193 GO:0018205 GO:0019538 GO:0031386 GO:0032446 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko03013 ko05418 map03013 map05418

Pfam Domains

Protein Families

Rad60-SLD

Protein Analysis

104

Amino Acids

11.5

Weight (kDa)

5.87

Isoelectric Point (pI)

34.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rad60-SLD	PF11976	20 - 90	1.1e-20	Ubiquitin-2 like Rad60 SUMO-like
ubiquitin	PF00240	23 - 91	4.6e-09	Ubiquitin family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0028685)

Species	Orthologous Gene IDs
malus_domestica	MD04G1010400.v1.1
pyrus_communis	pycom04g00850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	230
AclI	AACGTT	1 cut(s)	160
AclWI	GGATC	1 cut(s)	177
AcuI	CTGAAG	1 cut(s)	86
AgsI	TTSAA	1 cut(s)	301
AjnI	CCWGG	1 cut(s)	165
AluBI	AGCT	1 cut(s)	127
AluI	AGCT	1 cut(s)	127
Alw21I	GWGCWC	1 cut(s)	155
Alw44I	GTGCAC	1 cut(s)	151
AlwI	GGATC	1 cut(s)	177
ApaLI	GTGCAC	1 cut(s)	151
ApeKI	GCWGC	1 cut(s)	227
AseI	ATTAAT	2 cut(s)	60, 210
AsuHPI	GGTGA	1 cut(s)	263
BaeGI	GKGCMC	1 cut(s)	155
BbsI	GAAGAC	1 cut(s)	227
Bbv12I	GWGCWC	1 cut(s)	155
BbvI	GCAGC	1 cut(s)	214
BccI	CCATC	4 cut(s)	80, 191, 242, 275
BciT130I	CCWGG	1 cut(s)	167
BisI	GCNGC	1 cut(s)	228
BlsI	GCNGC	1 cut(s)	229
Bme1390I	CCNGG	1 cut(s)	167
BmrFI	CCNGG	1 cut(s)	167
BmsI	GCATC	2 cut(s)	133, 244
BpiI	GAAGAC	1 cut(s)	227
Bsa29I	ATCGAT	1 cut(s)	261
BsaBI	GATNNNNATC	1 cut(s)	201
BsaJI	CCNNGG	1 cut(s)	165
Bse8I	GATNNNNATC	1 cut(s)	201
BseBI	CCWGG	1 cut(s)	167
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	1 cut(s)	165
BseGI	GGATG	1 cut(s)	91
BseJI	GATNNNNATC	1 cut(s)	201
BseSI	GKGCMC	1 cut(s)	155
BseXI	GCAGC	1 cut(s)	214
BshVI	ATCGAT	1 cut(s)	261
BsiHKAI	GWGCWC	1 cut(s)	155
Bsp1286I	GDGCHC	1 cut(s)	155
Bsp143I	GATC	2 cut(s)	169, 202
BspACI	CCGC	1 cut(s)	230
BspDI	ATCGAT	1 cut(s)	261
BspPI	GGATC	1 cut(s)	177
BssECI	CCNNGG	1 cut(s)	165
BssMI	GATC	2 cut(s)	169, 202
Bst2UI	CCWGG	1 cut(s)	167
BstDEI	CTNAG	1 cut(s)	181
BstF5I	GGATG	1 cut(s)	91
BstKTI	GATC	2 cut(s)	172, 205
BstMBI	GATC	2 cut(s)	169, 202
BstNI	CCWGG	1 cut(s)	167
BstSCI	CCNGG	1 cut(s)	165
BstSLI	GKGCMC	1 cut(s)	155
BstV1I	GCAGC	1 cut(s)	214
BstV2I	GAAGAC	1 cut(s)	227
Bsu15I	ATCGAT	1 cut(s)	261
BsuTUI	ATCGAT	1 cut(s)	261
BtsCI	GGATG	1 cut(s)	91
BtsIMutI	CAGTG	2 cut(s)	276, 290
ClaI	ATCGAT	1 cut(s)	261
CviJI	RGCY	2 cut(s)	38, 127
CviKI_1	RGCY	2 cut(s)	38, 127
DdeI	CTNAG	1 cut(s)	181
DpnI	GATC	2 cut(s)	171, 204
DpnII	GATC	2 cut(s)	169, 202
Eco57I	CTGAAG	1 cut(s)	86
EcoRII	CCWGG	1 cut(s)	165
EcoT22I	ATGCAT	1 cut(s)	148
FaiI	YATR	2 cut(s)	240, 242
FauNDI	CATATG	1 cut(s)	240
Fnu4HI	GCNGC	1 cut(s)	228
FokI	GGATG	1 cut(s)	98
Fsp4HI	GCNGC	1 cut(s)	228
GluI	GCNGC	1 cut(s)	228
HphI	GGTGA	1 cut(s)	263
Hpy166II	GTNNAC	2 cut(s)	153, 290
Hpy188I	TCNGA	2 cut(s)	7, 66
Hpy188III	TCNNGA	2 cut(s)	29, 106
Hpy8I	GTNNAC	2 cut(s)	153, 290
HpyAV	CCTTC	1 cut(s)	61
HpyCH4IV	ACGT	1 cut(s)	160
HpyCH4V	TGCA	2 cut(s)	146, 153
HpyF3I	CTNAG	1 cut(s)	181
HpySE526I	ACGT	1 cut(s)	160
Kzo9I	GATC	2 cut(s)	169, 202
LmnI	GCTCC	1 cut(s)	232
LpnPI	CCDG	4 cut(s)	42, 91, 152, 179
Lsp1109I	GCAGC	1 cut(s)	214
LweI	GCATC	2 cut(s)	133, 244
MaeII	ACGT	1 cut(s)	160
MalI	GATC	2 cut(s)	171, 204
MboI	GATC	2 cut(s)	169, 202
MboII	GAAGA	4 cut(s)	184, 232, 257, 263
MhlI	GDGCHC	1 cut(s)	155
MluCI	AATT	1 cut(s)	175
MnlI	CCTC	2 cut(s)	22, 278
Mph1103I	ATGCAT	1 cut(s)	148
MseI	TTAA	5 cut(s)	60, 72, 78, 111, 210
MslI	CAYNNNNRTG	1 cut(s)	210
MspR9I	CCNGG	1 cut(s)	167
MvaI	CCWGG	1 cut(s)	167
NdeI	CATATG	1 cut(s)	240
NdeII	GATC	2 cut(s)	169, 202
NsiI	ATGCAT	1 cut(s)	148
PkrI	GCNGC	1 cut(s)	229
PshBI	ATTAAT	2 cut(s)	60, 210
Psp1406I	AACGTT	1 cut(s)	160
Psp6I	CCWGG	1 cut(s)	165
PspGI	CCWGG	1 cut(s)	165
RseI	CAYNNNNRTG	1 cut(s)	210
SaqAI	TTAA	5 cut(s)	60, 72, 78, 111, 210
SatI	GCNGC	1 cut(s)	228
Sau3AI	GATC	2 cut(s)	169, 202
ScrFI	CCNGG	1 cut(s)	167
SduI	GDGCHC	1 cut(s)	155
SetI	ASST	4 cut(s)	72, 129, 163, 289
SfaNI	GCATC	2 cut(s)	133, 244
SgeI	CNNG	7 cut(s)	27, 41, 94, 118, 178, 179, 229
SmiMI	CAYNNNNRTG	1 cut(s)	210
Sse9I	AATT	1 cut(s)	175
SsiI	CCGC	1 cut(s)	230
StyD4I	CCNGG	1 cut(s)	165
TaiI	ACGT	1 cut(s)	163
TaqI	TCGA	2 cut(s)	195, 261
TasI	AATT	1 cut(s)	175
Tru1I	TTAA	5 cut(s)	60, 72, 78, 111, 210
Tru9I	TTAA	5 cut(s)	60, 72, 78, 111, 210
TscAI	CASTG	2 cut(s)	283, 297
TseI	GCWGC	1 cut(s)	227
TspDTI	ATGAA	3 cut(s)	48, 178, 270
TspRI	CASTG	2 cut(s)	283, 297
VneI	GTGCAC	1 cut(s)	151
VspI	ATTAAT	2 cut(s)	60, 210
Zsp2I	ATGCAT	1 cut(s)	148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.