MD04G1040900.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0018338
Physical Location & Seq
Reverse (-)
4778868 .. 4785830
6963 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1040900.v1.1.491

Sequence Viewer

Length: 1350 bp
ATGTCTCTGATTGCTTACTTACAGGAGGGTTTTGGTGGTCCAATGTATAATCTCTGGGGGAATTTTTATGCTCAATGCAATGGGAATGGTCTTAGCCATAACGAGTACCCAAGAAAATACTCAGTTGACGATTCTTTGGGTTTGGCAAGAGATAGAACCATGGAAAATGTGCCTGAAACTGAAAGTAGGGAGATAAATTTCAGTGCATACCCTGCTCAAACTGCTGTTGATTGTTCCGTGGAAACTCCTACACAAGTTCCTTCCTCTTCTTTTGAGTTTCATGTTAGGTCAGAAGAGGGGATTAATCTTTATGTTGACTTCAATTCGAGCCTATCTGAGTGGACCAAGAGGTTCAAAAGTGAGGTTTGCACATGTCAAAATGTGCACAGAAACAAGTCTATGAGTCTTTATGAGGACCTTGGGTGCATTGGAAAAGGTGATAAAGAAAAGAGAAGTTCCTTCATACAGAACATAAATTATGGGTACATTGAAGATAAAGAACCGACAAAAAGTGACAATGTGGGCCTTGATCATTCTGTTAAACTAGCTGAAACTTCAGTAACCTCAGCAGTAAGATCGTGCAACATGGCTGCAGACGTGTCAGAGCATTTGGTGGAAGATCAAGCACTTGTCTCATCTAAACTTGACTCGGCTCCACTGAAGCAGATAATTTCTGGTGCTCATTCCTTTGCTATAGATGGCTCTTTAATCAAGCTTGATTCAAATGTCCTTAATAATTTCCAGAAGTCCATCTGTGCTCCTGCTGTCAACTCGTTATCAAAAGGTCAATTAAGTCCTGTCATAATGGAGCATCAAAATTCAAACCTCAATAATTGTCTGAATCCAACCCTGCAGAATAATTATAGCCCTGTCAACCTTAGTGTGGTATATCCTGGATGCTCAGCTAGTGCTTCTATAGAGATGCAGTCATCAGAGGTTGTGAGGTGCTCCAAAGACATATCATGTTCACCTTGTGGAAATGGAGGATTGGTGGGTCTGTTCGTACAAAAGGACAATTTAGAAACAGAACTTGGAATAATCAGCTCATGTGAGCAGGTTCCTGATCTGCTGGGGAACTACGTGCCAAAATCTGCTGAAGCGTGGGAGAGGAACAACATCACAAATGGGAGGGATAGTCCAGAATGCTCACAGTTAACCAGTTCAGTTGAGAAGAACAGTTTCTGTTACGATAATTTGGAATCTAATGAAGAACTTTCCAGAAAGAGGAAACATATAGAAGGTGAACTTCAAAGTGGCTATGGTAATCCGGATGCAAAGAATTTAACAAGTAGGAAGCATATTACTAGGAAAGTCCTTCCTAGAAGATCCGTGCGGCTGATCTCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

450

Amino Acids

49.54

Weight (kDa)

5.44

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018338)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G59430 AT3G59430 AT3G59430 AT3G59430 AT3G59430 AT3G59430 AT3G59430 AT3G59430
malus_domestica MD04G1040900.v1.1
pyrus_communis pycom04g03580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1045
AccIII TCCGGA 1 cut(s) 1267
AciI CCGC 1 cut(s) 1333
AclWI GGATC 1 cut(s) 1320
AcsI RAATTY 4 cut(s) 61, 196, 817, 1279
AcuI CTGAAG 3 cut(s) 540, 680, 1116
AdeI CACNNNGTG 1 cut(s) 974
AfaI GTAC 3 cut(s) 107, 485, 1005
AfiI CCNNNNNNNGG 2 cut(s) 883, 1224
AflIII ACRYGT 2 cut(s) 371, 597
AgsI TTSAA 6 cut(s) 322, 355, 491, 723, 822, 1250
AjiI CACGTC 1 cut(s) 598
AjnI CCWGG 1 cut(s) 892
AjuI GAANNNNNNNTTGG 2 cut(s) 1014, 1046
AluBI AGCT 4 cut(s) 548, 715, 905, 1044
AluI AGCT 4 cut(s) 548, 715, 905, 1044
Alw21I GWGCWC 4 cut(s) 387, 682, 760, 950
Alw26I GTCTC 2 cut(s) 9, 637
Alw44I GTGCAC 1 cut(s) 383
AlwI GGATC 1 cut(s) 1320
AlwNI CAGNNNCTG 1 cut(s) 1182
Aor13HI TCCGGA 1 cut(s) 1267
AoxI GGCC 1 cut(s) 523
ApaLI GTGCAC 1 cut(s) 383
ApeKI GCWGC 1 cut(s) 590
ApoI RAATTY 4 cut(s) 61, 196, 817, 1279
AseI ATTAAT 1 cut(s) 303
Asp700I GAANNNNTTC 1 cut(s) 1178
AspS9I GGNCC 4 cut(s) 38, 342, 415, 523
AsuHPI GGTGA 3 cut(s) 449, 960, 1253
AvaII GGWCC 3 cut(s) 38, 342, 415
BaeGI GKGCMC 1 cut(s) 387
Bbv12I GWGCWC 4 cut(s) 387, 682, 760, 950
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 1 cut(s) 577
BccI CCATC 2 cut(s) 692, 758
BcgI CGANNNNNNTGC 2 cut(s) 558, 592
BciT130I CCWGG 1 cut(s) 894
BclI TGATCA 1 cut(s) 529
BcoDI GTCTC 2 cut(s) 9, 637
BfaI CTAG 4 cut(s) 545, 906, 1305, 1320
BfmI CTRYAG 4 cut(s) 591, 693, 851, 915
BfuAI ACCTGC 1 cut(s) 1045
BisI GCNGC 2 cut(s) 591, 1334
BlpI GCTNAGC 1 cut(s) 901
BlsI GCNGC 2 cut(s) 592, 1335
Bme1390I CCNGG 1 cut(s) 894
Bme18I GGWCC 3 cut(s) 38, 342, 415
BmgBI CACGTC 1 cut(s) 598
BmgT120I GGNCC 4 cut(s) 38, 342, 415, 523
BmiI GGNNCC 2 cut(s) 654, 1059
BmrFI CCNGG 1 cut(s) 894
BmsI GCATC 4 cut(s) 820, 887, 912, 1261
Bpu10I CCTNAGC 1 cut(s) 565
Bpu1102I GCTNAGC 1 cut(s) 901
BsaAI YACGTR 1 cut(s) 1081
BsaJI CCNNGG 3 cut(s) 159, 237, 418
BsaWI WCCGGW 1 cut(s) 1267
BsaXI ACNNNNNCTCC 4 cut(s) 975, 1005, 1120, 1150
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 1224
Bse1I ACTGG 1 cut(s) 1158
Bse3DI GCAATG 1 cut(s) 85
BseAI TCCGGA 1 cut(s) 1267
BseBI CCWGG 1 cut(s) 894
BseDI CCNNGG 3 cut(s) 159, 237, 418
BseGI GGATG 2 cut(s) 902, 1276
BseLI CCNNNNNNNGG 2 cut(s) 883, 1224
BseMI GCAATG 1 cut(s) 85
BseMII CTCAG 4 cut(s) 135, 327, 579, 915
BseNI ACTGG 1 cut(s) 1158
BseSI GKGCMC 1 cut(s) 387
BseXI GCAGC 1 cut(s) 577
BseYI CCCAGC 1 cut(s) 1069
BshFI GGCC 1 cut(s) 525
BsiHKAI GWGCWC 4 cut(s) 387, 682, 760, 950
BsiSI CCGG 1 cut(s) 1268
BslI CCNNNNNNNGG 2 cut(s) 883, 1224
BsmAI GTCTC 2 cut(s) 9, 637
BsmI GAATGC 1 cut(s) 1148
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 4 cut(s) 387, 682, 760, 950
Bsp13I TCCGGA 1 cut(s) 1267
Bsp143I GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
Bsp1720I GCTNAGC 1 cut(s) 901
Bsp19I CCATGG 1 cut(s) 159
BspACI CCGC 1 cut(s) 1333
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 4 cut(s) 134, 328, 578, 914
BspEI TCCGGA 1 cut(s) 1267
BspLI GGNNCC 2 cut(s) 654, 1059
BspMAI CTGCAG 2 cut(s) 595, 855
BspMI ACCTGC 1 cut(s) 1045
BspPI GGATC 1 cut(s) 1320
BsrDI GCAATG 1 cut(s) 85
BsrI ACTGG 1 cut(s) 1158
BssECI CCNNGG 3 cut(s) 159, 237, 418
BssMI GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
BssT1I CCWWGG 2 cut(s) 159, 418
Bst2UI CCWGG 1 cut(s) 894
Bst4CI ACNGT 2 cut(s) 1152, 1178
Bst6I CTCTTC 2 cut(s) 271, 288
BstAPI GCANNNNNTGC 1 cut(s) 212
BstBAI YACGTR 1 cut(s) 1081
BstDEI CTNAG 7 cut(s) 92, 121, 336, 565, 878, 901, 1344
BstDSI CCRYGG 2 cut(s) 159, 237
BstF5I GGATG 2 cut(s) 902, 1276
BstKTI GATC 6 cut(s) 532, 578, 622, 1066, 1328, 1341
BstMAI GTCTC 2 cut(s) 9, 637
BstMBI GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
BstMWI GCNNNNNNNGC 2 cut(s) 212, 221
BstNI CCWGG 1 cut(s) 894
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 1 cut(s) 892
BstSFI CTRYAG 4 cut(s) 591, 693, 851, 915
BstSLI GKGCMC 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 577
BstX2I RGATCY 1 cut(s) 1325
BstYI RGATCY 1 cut(s) 1325
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 2 cut(s) 159, 237
BtrI CACGTC 1 cut(s) 598
BtsCI GGATG 2 cut(s) 902, 1276
BtsIMutI CAGTG 2 cut(s) 208, 656
BveI ACCTGC 1 cut(s) 1045
CaiI CAGNNNCTG 1 cut(s) 1182
Cfr13I GGNCC 4 cut(s) 38, 342, 415, 523
Csp6I GTAC 3 cut(s) 106, 484, 1004
CviAII CATG 6 cut(s) 160, 281, 372, 586, 963, 1047
CviQI GTAC 3 cut(s) 106, 484, 1004
DdeI CTNAG 7 cut(s) 92, 121, 336, 565, 878, 901, 1344
DpnI GATC 6 cut(s) 531, 577, 621, 1065, 1327, 1340
DpnII GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
DraIII CACNNNGTG 1 cut(s) 974
Eam1104I CTCTTC 2 cut(s) 271, 288
EarI CTCTTC 2 cut(s) 271, 288
Eco130I CCWWGG 2 cut(s) 159, 418
Eco47I GGWCC 3 cut(s) 38, 342, 415
Eco57I CTGAAG 3 cut(s) 540, 680, 1116
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 892
EcoT14I CCWWGG 2 cut(s) 159, 418
ErhI CCWWGG 2 cut(s) 159, 418
FaeI CATG 6 cut(s) 163, 284, 375, 589, 966, 1050
FalI AAGNNNNNCTT 2 cut(s) 1230, 1262
FatI CATG 6 cut(s) 159, 280, 371, 585, 962, 1046
FbaI TGATCA 1 cut(s) 529
Fnu4HI GCNGC 2 cut(s) 591, 1334
FokI GGATG 2 cut(s) 909, 1283
Fsp4HI GCNGC 2 cut(s) 591, 1334
FspBI CTAG 4 cut(s) 545, 906, 1305, 1320
GluI GCNGC 2 cut(s) 591, 1334
GsaI CCCAGC 1 cut(s) 1073
HaeIII GGCC 1 cut(s) 525
HapII CCGG 1 cut(s) 1268
Hin1II CATG 6 cut(s) 163, 284, 375, 589, 966, 1050
HincII GTYRAC 5 cut(s) 127, 316, 769, 874, 1155
HindII GTYRAC 5 cut(s) 127, 316, 769, 874, 1155
HindIII AAGCTT 1 cut(s) 713
HinfI GANTC 6 cut(s) 131, 403, 647, 719, 841, 1199
HpaI GTTAAC 1 cut(s) 1155
HpaII CCGG 1 cut(s) 1268
HphI GGTGA 3 cut(s) 449, 960, 1253
Hpy166II GTNNAC 9 cut(s) 127, 316, 342, 385, 769, 874, 968, 1155, 1244
Hpy188I TCNGA 6 cut(s) 9, 292, 337, 604, 840, 934
Hpy188III TCNNGA 5 cut(s) 742, 1061, 1139, 1218, 1268
Hpy8I GTNNAC 9 cut(s) 127, 316, 342, 385, 769, 874, 968, 1155, 1244
HpyAV CCTTC 4 cut(s) 270, 469, 1232, 1325
HpyCH4III ACNGT 2 cut(s) 1152, 1178
HpyCH4IV ACGT 2 cut(s) 597, 1080
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 221
HpyF3I CTNAG 7 cut(s) 92, 121, 336, 565, 878, 901, 1344
HpySE526I ACGT 2 cut(s) 597, 1080
Hsp92II CATG 6 cut(s) 163, 284, 375, 589, 966, 1050
Kpn2I TCCGGA 1 cut(s) 1267
Ksp22I TGATCA 1 cut(s) 529
KspAI GTTAAC 1 cut(s) 1155
Kzo9I GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
LmnI GCTCC 4 cut(s) 658, 763, 808, 953
Lsp1109I GCAGC 1 cut(s) 577
LweI GCATC 4 cut(s) 820, 887, 912, 1261
MaeI CTAG 4 cut(s) 545, 906, 1305, 1320
MaeII ACGT 2 cut(s) 597, 1080
MaeIII GTNAC 3 cut(s) 512, 559, 1184
MalI GATC 6 cut(s) 531, 577, 621, 1065, 1327, 1340
MboI GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
MboII GAAGA 7 cut(s) 258, 305, 503, 629, 1183, 1220, 1335
MflI RGATCY 1 cut(s) 1325
MhlI GDGCHC 4 cut(s) 387, 682, 760, 950
MlyI GAGTC 2 cut(s) 412, 641
MmeI TCCRAC 1 cut(s) 869
MroI TCCGGA 1 cut(s) 1267
MroXI GAANNNNTTC 1 cut(s) 1178
MseI TTAA 7 cut(s) 303, 540, 707, 732, 791, 1154, 1283
MspI CCGG 1 cut(s) 1268
MspR9I CCNGG 1 cut(s) 894
Mva1269I GAATGC 1 cut(s) 1148
MvaI CCWGG 1 cut(s) 894
MwoI GCNNNNNNNGC 2 cut(s) 212, 221
NcoI CCATGG 1 cut(s) 159
NdeII GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
NlaIII CATG 6 cut(s) 163, 284, 375, 589, 966, 1050
NlaIV GGNNCC 2 cut(s) 654, 1059
NmeAIII GCCGAG 1 cut(s) 629
NmuCI GTSAC 1 cut(s) 512
NspI RCATGY 1 cut(s) 375
PciI ACATGT 1 cut(s) 371
PctI GAATGC 1 cut(s) 1148
PdmI GAANNNNTTC 1 cut(s) 1178
PfeI GAWTC 4 cut(s) 131, 719, 841, 1199
PfoI TCCNGGA 1 cut(s) 892
PkrI GCNGC 2 cut(s) 592, 1335
PleI GAGTC 2 cut(s) 411, 641
PpsI GAGTC 2 cut(s) 411, 641
Ppu21I YACGTR 1 cut(s) 1081
PpuMI RGGWCCY 1 cut(s) 415
PscI ACATGT 1 cut(s) 371
PshBI ATTAAT 1 cut(s) 303
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 892
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 1 cut(s) 892
PspN4I GGNNCC 2 cut(s) 654, 1059
PspPI GGNCC 4 cut(s) 38, 342, 415, 523
PspPPI RGGWCCY 1 cut(s) 415
PstI CTGCAG 2 cut(s) 595, 855
PstNI CAGNNNCTG 1 cut(s) 1182
PsuI RGATCY 1 cut(s) 1325
RsaI GTAC 3 cut(s) 107, 485, 1005
RsaNI GTAC 3 cut(s) 106, 484, 1004
SaqAI TTAA 7 cut(s) 303, 540, 707, 732, 791, 1154, 1283
SatI GCNGC 2 cut(s) 591, 1334
Sau3AI GATC 6 cut(s) 529, 575, 619, 1063, 1325, 1338
Sau96I GGNCC 4 cut(s) 38, 342, 415, 523
SchI GAGTC 2 cut(s) 412, 641
ScrFI CCNGG 1 cut(s) 894
SduI GDGCHC 4 cut(s) 387, 682, 760, 950
SfaNI GCATC 4 cut(s) 820, 887, 912, 1261
SfcI CTRYAG 4 cut(s) 591, 693, 851, 915
SinI GGWCC 3 cut(s) 38, 342, 415
SsiI CCGC 1 cut(s) 1333
SspMI CTAG 4 cut(s) 545, 906, 1305, 1320
StyD4I CCNGG 1 cut(s) 892
StyI CCWWGG 2 cut(s) 159, 418
TaaI ACNGT 2 cut(s) 1152, 1178
TaiI ACGT 2 cut(s) 600, 1083
TaqI TCGA 1 cut(s) 326
TauI GCSGC 1 cut(s) 1336
TfiI GAWTC 4 cut(s) 131, 719, 841, 1199
Tru1I TTAA 7 cut(s) 303, 540, 707, 732, 791, 1154, 1283
Tru9I TTAA 7 cut(s) 303, 540, 707, 732, 791, 1154, 1283
TscAI CASTG 2 cut(s) 208, 663
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 1 cut(s) 590
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 3 cut(s) 269, 451, 1221
TspGWI ACGGA 2 cut(s) 226, 1318
TspRI CASTG 2 cut(s) 208, 663
VneI GTGCAC 1 cut(s) 383
VpaK11BI GGWCC 3 cut(s) 38, 342, 415
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 4 cut(s) 61, 196, 817, 1279
XceI RCATGY 1 cut(s) 375
XmnI GAANNNNTTC 1 cut(s) 1178
XspI CTAG 4 cut(s) 545, 906, 1305, 1320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.