MD04G1046000.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0028675
Physical Location & Seq
Reverse (-)
5332407 .. 5334295
1889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1046000.v1.1.491

Sequence Viewer

Length: 1281 bp
ATGCAAAGCACCAATCAGCAGCCCCAGAATAGTCACACATCAACAAGCCGTTCATGGGGGTCCAGCGAGCCTTGTCCGGAGGGTGCTAAATGGGCTTCAAGGCTTCTCATGGAGTGTGCAAGGGCAGTCTCAGAAAAAGACTCCAGTAAAATTAATCACCTTCTCTGGATGTTGAACGAGATGGCTTCTCCTTATGGGGACTGTGAGCAGAAACTGGCTTTTTATTTTTTGCAGGCTCTCTTTTGCAAGGCTACGGAATCAGGTCTCCGGTGCTACAAAACCCTAACCTCAGTAGCTGAAAAGAGTCACTCCTTTGATTCAGCCAGGAAGTTAATACTAAAGTTCCAAGAGGTAAGCCCTTGGACAACTTTTGGTCATGTGGCTTCAAACGGTGCTATATTGGAAGCCTTGGAGGGAGAAACCAAACTTCACATAATTGATATAAGCAATACCATTTGCACCCAGTGGCCTACTTTGTTAGAAGCTTTGGCCACGAGAAACGATGAGACGCCGCATTTGAAGCTCACCGTCGTGGTAACAGCAAGCATAGTCAAGTCAGTGATGAAGGAAATTGGCCAAAGAATGGAGAAATTTGCTAGGTTAATGGGTGTTCCCTTTGAGATTAATGTGATCAGCGGGTTAAACAATTTGGGAGAGCTCACAAAAGAGGATCTAGGTGTTCAGGAGGATGAGGCTGTTGCTGTGAATTGCATTGGGGCCTTGAGAAGAGTGGAAGTGGAAGAGAGAGGGGCGGTTATTCGGATGTTTCAATCGCTTCGGCCTCGCGTTGTGACTGTTGTTGAGGAAGAAGCTGATCTTTTCAGCTCATCAGTAAATCATGACTTTGTCAAGTGCTTTGAAGAGTGCCTTAGGTTTTACACTTTGTACTTTGACATGCTAGAGGAAAGCTTTGTCCCAACTAGCAATGAAAGACTGATGCTAGAGAGGGAGTGCTCGAGAAGCATAGTTAGGGTTTTGGGTTGTGATCATCACGATCACGATGATGACAAAAATGGTGGGGAGTGTGAGAGAAGGGAGAGAGGAAGCCAATGGTCTGACAGGCTCAAGGTGGCATTTTCGCCGGTTGGATTCAGCGACGATGTTGTGGATGATGTCAAGGCGTTGCTGAAGAGGTATCGAGCCGGCTGGGCACTAGTACTACCACCACCACAAGGAGGAGGAGAAGGAGGAGGGGATGATATTGATCAAACAGGAATATACTTAACATGGAAAGAGGAACCTGTAGTATGGGCTTCCATATGGAAACCCTCTGCTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GRAS

Protein Analysis

427

Amino Acids

47.81

Weight (kDa)

5.21

Isoelectric Point (pI)

47.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 34 - 422 1.7e-93 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0028675)

Species Orthologous Gene IDs
malus_domestica MD04G1046000.v1.1
prunus_persica Prupe.1G199300_v2.0.a1

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 583
AccII CGCG 1 cut(s) 786
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 3 cut(s) 512, 636, 752
AclWI GGATC 1 cut(s) 678
AcoI YGGCCR 2 cut(s) 489, 574
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 1148
AcyI GRCGYC 1 cut(s) 509
AdeI CACNNNGTG 1 cut(s) 465
AfaI GTAC 2 cut(s) 887, 1158
AfiI CCNNNNNNNGG 4 cut(s) 55, 583, 1172, 1175
AgsI TTSAA 6 cut(s) 99, 175, 387, 520, 770, 860
AhlI ACTAGT 1 cut(s) 1153
AjnI CCWGG 1 cut(s) 323
AleI CACNNNNGTG 1 cut(s) 530
AluBI AGCT 7 cut(s) 296, 485, 523, 658, 812, 825, 909
AluI AGCT 7 cut(s) 296, 485, 523, 658, 812, 825, 909
Alw21I GWGCWC 2 cut(s) 660, 956
Alw26I GTCTC 3 cut(s) 133, 269, 500
AlwI GGATC 1 cut(s) 678
AlwNI CAGNNNCTG 2 cut(s) 214, 296
Ama87I CYCGRG 1 cut(s) 955
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 5 cut(s) 467, 489, 574, 717, 779
ApeKI GCWGC 1 cut(s) 19
ApoI RAATTY 1 cut(s) 590
ArsI GACNNNNNNTTYG 2 cut(s) 499, 531
AseI ATTAAT 2 cut(s) 153, 624
AspS9I GGNCC 2 cut(s) 60, 717
AsuHPI GGTGA 2 cut(s) 149, 517
AvaI CYCGRG 1 cut(s) 955
AvaII GGWCC 1 cut(s) 60
AxyI CCTNAGG 1 cut(s) 869
BaeGI GKGCMC 1 cut(s) 1153
BalI TGGCCA 2 cut(s) 491, 576
BanII GRGCYC 1 cut(s) 660
BauI CACGAG 1 cut(s) 493
Bbv12I GWGCWC 2 cut(s) 660, 956
BbvI GCAGC 1 cut(s) 31
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 33
BciT130I CCWGG 1 cut(s) 325
BclI TGATCA 3 cut(s) 630, 985, 1204
BcoDI GTCTC 3 cut(s) 133, 269, 500
BcuI ACTAGT 1 cut(s) 1153
BfaI CTAG 7 cut(s) 597, 674, 899, 921, 941, 1154, 1275
BfmI CTRYAG 1 cut(s) 1242
BglI GCCNNNNNGGC 1 cut(s) 1148
BisI GCNGC 2 cut(s) 20, 512
BlsI GCNGC 2 cut(s) 21, 513
BmcAI AGTACT 1 cut(s) 1158
Bme1390I CCNGG 1 cut(s) 325
Bme18I GGWCC 1 cut(s) 60
BmeT110I CYCGRG 1 cut(s) 955
BmgT120I GGNCC 2 cut(s) 60, 717
BmiI GGNNCC 3 cut(s) 61, 718, 1239
BmrFI CCNGG 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 457
BmsI GCATC 1 cut(s) 927
BmuI ACTGGG 1 cut(s) 457
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 742, 1049
BsaBI GATNNNNATC 1 cut(s) 1203
BsaHI GRCGYC 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 359, 408
BsaWI WCCGGW 2 cut(s) 76, 267
Bsc4I CCNNNNNNNGG 4 cut(s) 55, 583, 1172, 1175
Bse118I RCCGGY 2 cut(s) 1081, 1142
Bse1I ACTGG 3 cut(s) 144, 219, 463
Bse21I CCTNAGG 1 cut(s) 869
Bse3DI GCAATG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 1203
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 1 cut(s) 325
BseDI CCNNGG 2 cut(s) 359, 408
BseGI GGATG 5 cut(s) 174, 694, 768, 1114, 1201
BseJI GATNNNNATC 1 cut(s) 1203
BseLI CCNNNNNNNGG 4 cut(s) 55, 583, 1172, 1175
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 2 cut(s) 144, 303
BseNI ACTGG 3 cut(s) 144, 219, 463
BseRI GAGGAG 3 cut(s) 1191, 1194, 1203
BseSI GKGCMC 1 cut(s) 1153
BseXI GCAGC 1 cut(s) 31
BseYI CCCAGC 1 cut(s) 1146
Bsh1236I CGCG 1 cut(s) 786
BshFI GGCC 5 cut(s) 469, 491, 576, 719, 781
BsiHKAI GWGCWC 2 cut(s) 660, 956
BsiHKCI CYCGRG 1 cut(s) 955
BsiSI CCGG 4 cut(s) 77, 268, 1082, 1143
BslFI GGGAC 2 cut(s) 212, 899
BslI CCNNNNNNNGG 4 cut(s) 55, 583, 1172, 1175
BsmAI GTCTC 3 cut(s) 133, 269, 500
BsmBI CGTCTC 1 cut(s) 500
BsmFI GGGAC 2 cut(s) 212, 899
BsnI GGCC 5 cut(s) 469, 491, 576, 719, 781
Bso31I GGTCTC 1 cut(s) 269
BsoBI CYCGRG 1 cut(s) 955
Bsp1286I GDGCHC 3 cut(s) 660, 956, 1153
Bsp13I TCCGGA 1 cut(s) 76
Bsp143I GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
BspACI CCGC 3 cut(s) 512, 636, 752
BspANI GGCC 5 cut(s) 469, 491, 576, 719, 781
BspCNI CTCAG 2 cut(s) 143, 302
BspEI TCCGGA 1 cut(s) 76
BspFNI CGCG 1 cut(s) 786
BspHI TCATGA 1 cut(s) 838
BspLI GGNNCC 3 cut(s) 61, 718, 1239
BspPI GGATC 1 cut(s) 678
BspTNI GGTCTC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 931
BsrFI RCCGGY 2 cut(s) 1081, 1142
BsrI ACTGG 3 cut(s) 144, 219, 463
BssAI RCCGGY 2 cut(s) 1081, 1142
BssECI CCNNGG 2 cut(s) 359, 408
BssMI GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
BssNI GRCGYC 1 cut(s) 509
BssSI CACGAG 1 cut(s) 493
BssT1I CCWWGG 2 cut(s) 359, 408
Bst2BI CACGAG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 325
Bst4CI ACNGT 4 cut(s) 203, 392, 529, 796
Bst6I CTCTTC 4 cut(s) 721, 735, 855, 1124
BstACI GRCGYC 1 cut(s) 509
BstC8I GCNNGC 4 cut(s) 68, 234, 544, 1144
BstDEI CTNAG 3 cut(s) 130, 289, 869
BstF5I GGATG 5 cut(s) 174, 694, 768, 1114, 1201
BstFNI CGCG 1 cut(s) 786
BstKTI GATC 6 cut(s) 633, 673, 817, 988, 997, 1207
BstMAI GTCTC 3 cut(s) 133, 269, 500
BstMBI GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
BstMWI GCNNNNNNNGC 4 cut(s) 92, 520, 960, 1148
BstNI CCWGG 1 cut(s) 325
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 323
BstSFI CTRYAG 1 cut(s) 1242
BstSLI GKGCMC 1 cut(s) 1153
BstUI CGCG 1 cut(s) 786
BstV1I GCAGC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
Bsu36I CCTNAGG 1 cut(s) 869
BsuRI GGCC 5 cut(s) 469, 491, 576, 719, 781
BtsCI GGATG 5 cut(s) 174, 694, 768, 1114, 1201
BtsIMutI CAGTG 2 cut(s) 470, 564
Cac8I GCNNGC 4 cut(s) 68, 234, 544, 1144
CaiI CAGNNNCTG 2 cut(s) 214, 296
CciI TCATGA 1 cut(s) 838
Cfr10I RCCGGY 2 cut(s) 1081, 1142
Cfr13I GGNCC 2 cut(s) 60, 717
CseI GACGC 1 cut(s) 517
Csp6I GTAC 2 cut(s) 886, 1157
CspCI CAANNNNNGTGG 2 cut(s) 997, 1032
CviAII CATG 6 cut(s) 54, 109, 377, 839, 895, 1227
CviQI GTAC 2 cut(s) 886, 1157
DdeI CTNAG 3 cut(s) 130, 289, 869
DpnI GATC 6 cut(s) 632, 672, 816, 987, 996, 1206
DpnII GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
DraIII CACNNNGTG 1 cut(s) 465
EaeI YGGCCR 2 cut(s) 489, 574
Eam1104I CTCTTC 4 cut(s) 721, 735, 855, 1124
EarI CTCTTC 4 cut(s) 721, 735, 855, 1124
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 2 cut(s) 359, 408
Eco24I GRGCYC 1 cut(s) 660
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 1 cut(s) 60
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 1 cut(s) 1148
Eco81I CCTNAGG 1 cut(s) 869
Eco88I CYCGRG 1 cut(s) 955
EcoICRI GAGCTC 1 cut(s) 658
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 323
EcoT14I CCWWGG 2 cut(s) 359, 408
EcoT38I GRGCYC 1 cut(s) 660
ErhI CCWWGG 2 cut(s) 359, 408
Esp3I CGTCTC 1 cut(s) 500
FaeI CATG 6 cut(s) 57, 112, 380, 842, 898, 1230
FalI AAGNNNNNCTT 4 cut(s) 801, 833, 852, 884
FaqI GGGAC 2 cut(s) 212, 899
FatI CATG 6 cut(s) 53, 108, 376, 838, 894, 1226
FauI CCCGC 1 cut(s) 629
FauNDI CATATG 1 cut(s) 1259
FbaI TGATCA 3 cut(s) 630, 985, 1204
Fnu4HI GCNGC 2 cut(s) 20, 512
FokI GGATG 5 cut(s) 181, 701, 775, 1121, 1208
FriOI GRGCYC 1 cut(s) 660
Fsp4HI GCNGC 2 cut(s) 20, 512
FspBI CTAG 7 cut(s) 597, 674, 899, 921, 941, 1154, 1275
GluI GCNGC 2 cut(s) 20, 512
GsaI CCCAGC 1 cut(s) 1150
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 5 cut(s) 469, 491, 576, 719, 781
HapII CCGG 4 cut(s) 77, 268, 1082, 1143
HgaI GACGC 1 cut(s) 517
Hin1I GRCGYC 1 cut(s) 509
Hin1II CATG 6 cut(s) 57, 112, 380, 842, 898, 1230
HindIII AAGCTT 2 cut(s) 483, 907
HinfI GANTC 5 cut(s) 140, 257, 304, 317, 1089
HpaII CCGG 4 cut(s) 77, 268, 1082, 1143
HphI GGTGA 2 cut(s) 149, 517
Hpy188I TCNGA 3 cut(s) 133, 762, 1057
Hpy188III TCNNGA 7 cut(s) 77, 166, 683, 839, 957, 992, 998
Hpy99I CGWCG 2 cut(s) 533, 1100
HpyAV CCTTC 4 cut(s) 170, 559, 1026, 1178
HpyCH4III ACNGT 4 cut(s) 203, 392, 529, 796
HpyCH4V TGCA 6 cut(s) 4, 119, 232, 246, 459, 711
HpyF10VI GCNNNNNNNGC 4 cut(s) 92, 520, 960, 1148
HpyF3I CTNAG 3 cut(s) 130, 289, 869
Hsp92I GRCGYC 1 cut(s) 509
Hsp92II CATG 6 cut(s) 57, 112, 380, 842, 898, 1230
Kpn2I TCCGGA 1 cut(s) 76
KroI GCCGGC 1 cut(s) 1142
KroNI GCCGGC 1 cut(s) 1144
Ksp22I TGATCA 3 cut(s) 630, 985, 1204
Kzo9I GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
Lsp1109I GCAGC 1 cut(s) 31
LweI GCATC 1 cut(s) 927
MaeI CTAG 7 cut(s) 597, 674, 899, 921, 941, 1154, 1275
MaeIII GTNAC 4 cut(s) 32, 305, 535, 790
MalI GATC 6 cut(s) 632, 672, 816, 987, 996, 1206
MboI GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
MboII GAAGA 5 cut(s) 738, 752, 818, 872, 1141
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 3 cut(s) 660, 956, 1153
MlsI TGGCCA 2 cut(s) 491, 576
MluCI AATT 6 cut(s) 150, 435, 570, 590, 646, 706
MluNI TGGCCA 2 cut(s) 491, 576
MlyI GAGTC 2 cut(s) 134, 313
MmeI TCCRAC 1 cut(s) 1066
Mox20I TGGCCA 2 cut(s) 491, 576
MroI TCCGGA 1 cut(s) 76
MroNI GCCGGC 1 cut(s) 1142
MscI TGGCCA 2 cut(s) 491, 576
MseI TTAA 7 cut(s) 153, 332, 602, 624, 641, 1223, 1279
MslI CAYNNNNRTG 2 cut(s) 530, 1002
Msp20I TGGCCA 2 cut(s) 491, 576
MspA1I CMGCKG 1 cut(s) 636
MspI CCGG 4 cut(s) 77, 268, 1082, 1143
MspR9I CCNGG 1 cut(s) 325
MvaI CCWGG 1 cut(s) 325
MvnI CGCG 1 cut(s) 786
MwoI GCNNNNNNNGC 4 cut(s) 92, 520, 960, 1148
NaeI GCCGGC 1 cut(s) 1144
NdeI CATATG 1 cut(s) 1259
NdeII GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
NgoMIV GCCGGC 1 cut(s) 1142
NlaIII CATG 6 cut(s) 57, 112, 380, 842, 898, 1230
NlaIV GGNNCC 3 cut(s) 61, 718, 1239
NmuCI GTSAC 3 cut(s) 32, 305, 790
NspI RCATGY 1 cut(s) 898
OliI CACNNNNGTG 1 cut(s) 530
PaeR7I CTCGAG 1 cut(s) 955
PagI TCATGA 1 cut(s) 838
PdiI GCCGGC 1 cut(s) 1144
PfeI GAWTC 3 cut(s) 257, 317, 1089
PflFI GACNNNGTC 1 cut(s) 845
PflMI CCANNNNNTGG 1 cut(s) 583
PkrI GCNGC 2 cut(s) 21, 513
PleI GAGTC 2 cut(s) 134, 312
PpsI GAGTC 2 cut(s) 134, 312
PshBI ATTAAT 2 cut(s) 153, 624
Psp124BI GAGCTC 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 323
PspFI CCCAGC 1 cut(s) 1146
PspGI CCWGG 1 cut(s) 323
PspN4I GGNNCC 3 cut(s) 61, 718, 1239
PspPI GGNCC 2 cut(s) 60, 717
PstNI CAGNNNCTG 2 cut(s) 214, 296
PsuI RGATCY 1 cut(s) 670
PsyI GACNNNGTC 1 cut(s) 845
RsaI GTAC 2 cut(s) 887, 1158
RsaNI GTAC 2 cut(s) 886, 1157
RseI CAYNNNNRTG 2 cut(s) 530, 1002
SacI GAGCTC 1 cut(s) 660
SaqAI TTAA 7 cut(s) 153, 332, 602, 624, 641, 1223, 1279
SatI GCNGC 2 cut(s) 20, 512
Sau3AI GATC 6 cut(s) 630, 670, 814, 985, 994, 1204
Sau96I GGNCC 2 cut(s) 60, 717
ScaI AGTACT 1 cut(s) 1158
SchI GAGTC 2 cut(s) 134, 313
ScrFI CCNGG 1 cut(s) 325
SduI GDGCHC 3 cut(s) 660, 956, 1153
SfaNI GCATC 1 cut(s) 927
SfcI CTRYAG 1 cut(s) 1242
Sfr274I CTCGAG 1 cut(s) 955
SinI GGWCC 1 cut(s) 60
SlaI CTCGAG 1 cut(s) 955
SmiMI CAYNNNNRTG 2 cut(s) 530, 1002
SmlI CTYRAG 3 cut(s) 721, 955, 1064
SmoI CTYRAG 3 cut(s) 721, 955, 1064
SpeI ACTAGT 1 cut(s) 1153
Sse9I AATT 6 cut(s) 150, 435, 570, 590, 646, 706
SsiI CCGC 3 cut(s) 512, 636, 752
SspMI CTAG 7 cut(s) 597, 674, 899, 921, 941, 1154, 1275
SstI GAGCTC 1 cut(s) 660
StyD4I CCNGG 1 cut(s) 323
StyI CCWWGG 2 cut(s) 359, 408
TaaI ACNGT 4 cut(s) 203, 392, 529, 796
TaqI TCGA 2 cut(s) 956, 1138
TasI AATT 6 cut(s) 150, 435, 570, 590, 646, 706
TatI WGTACW 2 cut(s) 885, 1156
TauI GCSGC 1 cut(s) 514
TfiI GAWTC 3 cut(s) 257, 317, 1089
Tru1I TTAA 7 cut(s) 153, 332, 602, 624, 641, 1223, 1279
Tru9I TTAA 7 cut(s) 153, 332, 602, 624, 641, 1223, 1279
TscAI CASTG 2 cut(s) 470, 564
TseFI GTSAC 3 cut(s) 32, 305, 790
TseI GCWGC 1 cut(s) 19
Tsp45I GTSAC 3 cut(s) 32, 305, 790
TspDTI ATGAA 3 cut(s) 42, 578, 942
TspGWI ACGGA 1 cut(s) 269
TspRI CASTG 2 cut(s) 470, 564
Tth111I GACNNNGTC 1 cut(s) 845
Van91I CCANNNNNTGG 1 cut(s) 583
VpaK11BI GGWCC 1 cut(s) 60
VspI ATTAAT 2 cut(s) 153, 624
XapI RAATTY 1 cut(s) 590
XceI RCATGY 1 cut(s) 898
XhoI CTCGAG 1 cut(s) 955
XspI CTAG 7 cut(s) 597, 674, 899, 921, 941, 1154, 1275
ZrmI AGTACT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.