MD04G1089000.v1.1

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0021188
Physical Location & Seq
Reverse (-)
15420869 .. 15423355
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1089000.v1.1.491

Sequence Viewer

Length: 540 bp
ATGAGCTTGATGCTCACGCGCCTCCCCACCTTCGCGCCATCGCAGACGCCTCTCAGCGGGGCGACCTCGACGCCCTTCGCCTCGCTCTCGGTAGCATTGATGATTCAGTGGAAGATGGAGACACAGCTCTCCATCTGGCCTGTCTATATGGTAATCTTTCTTGTGTCCGGAATAATTCTTCGGATGTGGTTACTTTTGGAGAGAGGAGCAAATTTGGAGGCTAAGGATGAAGATGGAGCAATTCCTTTGCATGATGCTTGTGCTGGGGGATACACCGAAGTGGTTCAACTTTTAATTAACAGTGCTAATGATGCTGAGCGTGTAAATAAAATGCTAGAAACAGTTGATGCTGAAGGTAATACTCCTCTTCATCATGCAGCAAGAGGTGAACATGCTGATATCATAAGATTGTTGCTGGCATCTGGAGCTTTGCCTACCAAGGCGAACCTATATGGAAAGATCCCAAGTGACCTACCAGAGGCCGACACAGAAGCTAGAAGACTTTTGGACGCTGCCTCTGCTGGTATGGCATGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000209 GO:0000242 GO:0000723 GO:0003674 GO:0003824 GO:0003950 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005813 GO:0005815 GO:0005856 GO:0006139 GO:0006259 GO:0006464 GO:0006471 GO:0006725 GO:0006807 GO:0006996 GO:0008104 GO:0008150 GO:0008152 GO:0009892 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010604 GO:0010605 GO:0010638 GO:0010639 GO:0010646 GO:0010647 GO:0012505 GO:0015630 GO:0016043 GO:0016567 GO:0016740 GO:0016757 GO:0016763 GO:0019219 GO:0019222 GO:0019538 GO:0019899 GO:0023051 GO:0023056 GO:0030111 GO:0030177 GO:0031323 GO:0031324 GO:0031325 GO:0031967 GO:0031975 GO:0032200 GO:0032204 GO:0032205 GO:0032206 GO:0032446 GO:0032501 GO:0032502 GO:0033036 GO:0033043 GO:0033044 GO:0033365 GO:0034502 GO:0034613 GO:0034641 GO:0035264 GO:0036211 GO:0040007 GO:0040008 GO:0040014 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044430 GO:0044444 GO:0044446 GO:0044450 GO:0044464 GO:0045934 GO:0045935 GO:0046483 GO:0048471 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048589 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0051052 GO:0051053 GO:0051054 GO:0051128 GO:0051129 GO:0051130 GO:0051171 GO:0051172 GO:0051173 GO:0051179 GO:0051239 GO:0051276 GO:0051641 GO:0060249 GO:0060255 GO:0060828 GO:0065007 GO:0065008 GO:0070198 GO:0070213 GO:0070647 GO:0070727 GO:0071704 GO:0071840 GO:0080090 GO:0090263 GO:0090304 GO:1901360 GO:1901564 GO:1904353 GO:1904355 GO:1904356 GO:1904357 GO:2001251 GO:2001252
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

180

Amino Acids

19.31

Weight (kDa)

5.0

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 64 - 146 1.9e-14 Ankyrin repeats (3 copies)
Ank_KRIT1 PF24521 82 - 153 2.9e-06 KRIT1 ankyrin-repeats domain
Ank PF00023 119 - 147 3.2e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021188)

Species Orthologous Gene IDs
malus_domestica MD04G1089000.v1.1
rosa_chinensis RchiOBHm_Chr3g0470861
rosa_laevigata RLG00000024239
rosa_samantha Rh3BG188100 Rh3CG178100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 19, 35
AccIII TCCGGA 1 cut(s) 167
AciI CCGC 1 cut(s) 57
AclWI GGATC 1 cut(s) 454
AcsI RAATTY 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 372
AcyI GRCGYC 2 cut(s) 47, 71
AfiI CCNNNNNNNGG 2 cut(s) 56, 478
AgsI TTSAA 1 cut(s) 287
AleI CACNNNNGTG 1 cut(s) 278
AluBI AGCT 4 cut(s) 6, 127, 428, 494
AluI AGCT 4 cut(s) 6, 127, 428, 494
Alw26I GTCTC 1 cut(s) 113
AlwI GGATC 1 cut(s) 454
Aor13HI TCCGGA 1 cut(s) 167
AoxI GGCC 2 cut(s) 137, 480
ApeKI GCWGC 2 cut(s) 377, 512
ApoI RAATTY 1 cut(s) 211
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 2 cut(s) 21, 37
AsuHPI GGTGA 1 cut(s) 398
BbsI GAAGAC 1 cut(s) 505
BbvI GCAGC 2 cut(s) 389, 499
BccI CCATC 4 cut(s) 46, 109, 140, 227
BciVI GTATCC 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 113
BfaI CTAG 2 cut(s) 335, 495
BfuI GTATCC 1 cut(s) 263
BisI GCNGC 2 cut(s) 378, 513
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 2 cut(s) 379, 514
BmsI GCATC 4 cut(s) 244, 301, 337, 428
BpiI GAAGAC 1 cut(s) 505
BpmI CTGGAG 1 cut(s) 444
Bpu10I CCTNAGC 1 cut(s) 222
Bpu1102I GCTNAGC 1 cut(s) 315
BsaHI GRCGYC 2 cut(s) 47, 71
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 1 cut(s) 167
Bsc4I CCNNNNNNNGG 2 cut(s) 56, 478
Bse1I ACTGG 1 cut(s) 535
BseAI TCCGGA 1 cut(s) 167
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 2 cut(s) 189, 232
BseLI CCNNNNNNNGG 2 cut(s) 56, 478
BseMII CTCAG 2 cut(s) 67, 306
BseNI ACTGG 1 cut(s) 535
BseRI GAGGAG 2 cut(s) 219, 354
BseXI GCAGC 2 cut(s) 389, 499
BseYI CCCAGC 1 cut(s) 263
Bsh1236I CGCG 2 cut(s) 19, 35
BshFI GGCC 2 cut(s) 139, 482
BsiSI CCGG 1 cut(s) 168
BslI CCNNNNNNNGG 2 cut(s) 56, 478
BsmAI GTCTC 1 cut(s) 113
BsnI GGCC 2 cut(s) 139, 482
Bsp13I TCCGGA 1 cut(s) 167
Bsp143I GATC 1 cut(s) 459
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 139, 482
BspCNI CTCAG 2 cut(s) 66, 307
BspEI TCCGGA 1 cut(s) 167
BspFNI CGCG 2 cut(s) 19, 35
BspPI GGATC 1 cut(s) 454
BsrI ACTGG 1 cut(s) 535
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 1 cut(s) 459
BssNI GRCGYC 2 cut(s) 47, 71
BssT1I CCWWGG 1 cut(s) 438
Bst4CI ACNGT 2 cut(s) 302, 343
Bst6I CTCTTC 1 cut(s) 372
BstACI GRCGYC 2 cut(s) 47, 71
BstC8I GCNNGC 2 cut(s) 417, 532
BstDEI CTNAG 3 cut(s) 53, 222, 315
BstENI CCTNNNNNAGG 1 cut(s) 476
BstF5I GGATG 2 cut(s) 189, 232
BstFNI CGCG 2 cut(s) 19, 35
BstHHI GCGC 2 cut(s) 21, 37
BstKTI GATC 1 cut(s) 462
BstMAI GTCTC 1 cut(s) 113
BstMBI GATC 1 cut(s) 459
BstMWI GCNNNNNNNGC 4 cut(s) 311, 425, 518, 527
BstNSI RCATGY 2 cut(s) 395, 534
BstUI CGCG 2 cut(s) 19, 35
BstV1I GCAGC 2 cut(s) 389, 499
BstV2I GAAGAC 1 cut(s) 505
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuI GTATCC 1 cut(s) 263
BsuRI GGCC 2 cut(s) 139, 482
BtgZI GCGATG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 189, 232
BtsIMutI CAGTG 2 cut(s) 113, 307
Cac8I GCNNGC 2 cut(s) 417, 532
CfoI GCGC 2 cut(s) 21, 37
CseI GACGC 3 cut(s) 55, 79, 518
CviAII CATG 4 cut(s) 251, 374, 392, 531
CviJI RGCY 7 cut(s) 6, 127, 139, 221, 428, 482, 494
CviKI_1 RGCY 7 cut(s) 6, 127, 139, 221, 428, 482, 494
DdeI CTNAG 3 cut(s) 53, 222, 315
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
Eam1104I CTCTTC 1 cut(s) 372
EarI CTCTTC 1 cut(s) 372
Eco130I CCWWGG 1 cut(s) 438
Eco32I GATATC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 372
EcoNI CCTNNNNNAGG 1 cut(s) 476
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 4 cut(s) 254, 377, 395, 534
FatI CATG 4 cut(s) 250, 373, 391, 530
FauI CCCGC 1 cut(s) 50
Fnu4HI GCNGC 2 cut(s) 378, 513
FokI GGATG 2 cut(s) 196, 239
Fsp4HI GCNGC 2 cut(s) 378, 513
FspBI CTAG 2 cut(s) 335, 495
GlaI GCGC 2 cut(s) 20, 36
GluI GCNGC 2 cut(s) 378, 513
GsaI CCCAGC 1 cut(s) 267
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 2 cut(s) 139, 482
HapII CCGG 1 cut(s) 168
HgaI GACGC 3 cut(s) 55, 79, 518
HhaI GCGC 2 cut(s) 21, 37
Hin1I GRCGYC 2 cut(s) 47, 71
Hin1II CATG 4 cut(s) 254, 377, 395, 534
Hin6I GCGC 2 cut(s) 19, 35
HinP1I GCGC 2 cut(s) 19, 35
HinfI GANTC 1 cut(s) 103
HpaII CCGG 1 cut(s) 168
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 1 cut(s) 183
Hpy188III TCNNGA 2 cut(s) 168, 423
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 1 cut(s) 73
HpyAV CCTTC 3 cut(s) 40, 85, 347
HpyCH4III ACNGT 2 cut(s) 302, 343
HpyCH4V TGCA 2 cut(s) 250, 377
HpyF10VI GCNNNNNNNGC 4 cut(s) 311, 425, 518, 527
HpyF3I CTNAG 3 cut(s) 53, 222, 315
Hsp92I GRCGYC 2 cut(s) 47, 71
Hsp92II CATG 4 cut(s) 254, 377, 395, 534
HspAI GCGC 2 cut(s) 19, 35
Kpn2I TCCGGA 1 cut(s) 167
Kzo9I GATC 1 cut(s) 459
LmnI GCTCC 3 cut(s) 206, 236, 425
LpnPI CCDG 8 cut(s) 121, 153, 181, 249, 401, 408, 489, 507
Lsp1109I GCAGC 2 cut(s) 389, 499
LweI GCATC 4 cut(s) 244, 301, 337, 428
MaeI CTAG 2 cut(s) 335, 495
MaeIII GTNAC 2 cut(s) 189, 467
MalI GATC 1 cut(s) 461
MboI GATC 1 cut(s) 459
MboII GAAGA 5 cut(s) 124, 170, 242, 359, 510
MflI RGATCY 1 cut(s) 459
MluCI AATT 4 cut(s) 174, 211, 240, 294
MroI TCCGGA 1 cut(s) 167
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 2 cut(s) 293, 297
MslI CAYNNNNRTG 1 cut(s) 278
MspA1I CMGCKG 1 cut(s) 57
MspI CCGG 1 cut(s) 168
MvnI CGCG 2 cut(s) 19, 35
MwoI GCNNNNNNNGC 4 cut(s) 311, 425, 518, 527
NdeII GATC 1 cut(s) 459
NlaIII CATG 4 cut(s) 254, 377, 395, 534
NmuCI GTSAC 1 cut(s) 467
NspI RCATGY 2 cut(s) 395, 534
OliI CACNNNNGTG 1 cut(s) 278
PacI TTAATTAA 1 cut(s) 297
PaeI GCATGC 1 cut(s) 534
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 379, 514
PspFI CCCAGC 1 cut(s) 263
PsuI RGATCY 1 cut(s) 459
RseI CAYNNNNRTG 1 cut(s) 278
SaqAI TTAA 2 cut(s) 293, 297
SatI GCNGC 2 cut(s) 378, 513
Sau3AI GATC 1 cut(s) 459
SfaNI GCATC 4 cut(s) 244, 301, 337, 428
SmiMI CAYNNNNRTG 1 cut(s) 278
SphI GCATGC 1 cut(s) 534
Sse9I AATT 4 cut(s) 174, 211, 240, 294
SsiI CCGC 1 cut(s) 57
SspMI CTAG 2 cut(s) 335, 495
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 2 cut(s) 302, 343
TaqI TCGA 1 cut(s) 68
TasI AATT 4 cut(s) 174, 211, 240, 294
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 293, 297
Tru9I TTAA 2 cut(s) 293, 297
TscAI CASTG 2 cut(s) 113, 307
TseFI GTSAC 1 cut(s) 467
TseI GCWGC 2 cut(s) 377, 512
Tsp45I GTSAC 1 cut(s) 467
TspDTI ATGAA 2 cut(s) 243, 359
TspRI CASTG 2 cut(s) 113, 307
XagI CCTNNNNNAGG 1 cut(s) 476
XapI RAATTY 1 cut(s) 211
XceI RCATGY 2 cut(s) 395, 534
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 2 cut(s) 335, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.