RLG00000024239

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0021188
Physical Location & Seq
Reverse (-)
33397859 .. 33398593
735 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024239

Sequence Viewer

Length: 447 bp
ATGGAGTCTCTAGGATTGGCGCCGGATAACTCCTCTCTCCCTCTCGTTCTCAAAGCCTGTGCGCGGCCGAGCGCCGTCGAGAGAGGGAAGGGAATACATTATTCGAGTATTCGGAACTCGGGTTTCGATAAAGATGTGAGGATTGGGACTGCCCTTGTTGATTTCTACTGCAAAAGATACACTGAGATAGTTCAATTTCTAGTTAACAGTGCTAATGACACCGAGCGTGTAAAGAGAATGCTAGAAGCAGTTGACGCTGAAGGTGATACTCCTCTGCATCATGCAGCCAGAGGTGAGCACGCTGATATTATAAGATTGTTGCTGGCTTCTGGAGCTTCTCCTACAAAGGCAAATCTGTACGGAAAGATCCCAAGTGAGTTACCAGAACCGAACATAGAAGCTAAGGGGATTTTGGAGGCTGCTGCTGGTGCTATGGCATGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_3 Ank_4 Ank_5

Protein Analysis

149

Amino Acids

15.87

Weight (kDa)

6.08

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 49 - 116 1.8e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 61 - 108 2.6e-06 Ankyrin repeats (many copies)
Ank PF00023 88 - 118 8.4e-08 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021188)

Species Orthologous Gene IDs
malus_domestica MD04G1089000.v1.1
rosa_chinensis RchiOBHm_Chr3g0470861
rosa_laevigata RLG00000024239
rosa_samantha Rh3BG188100 Rh3CG178100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 19
AccII CGCG 1 cut(s) 64
AciI CCGC 1 cut(s) 64
AclWI GGATC 1 cut(s) 361
AcoI YGGCCR 1 cut(s) 65
AcuI CTGAAG 1 cut(s) 279
AcyI GRCGYC 1 cut(s) 20
AfaI GTAC 1 cut(s) 359
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 1 cut(s) 194
AluBI AGCT 2 cut(s) 335, 401
AluI AGCT 2 cut(s) 335, 401
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 12
AlwI GGATC 1 cut(s) 361
Ama87I CYCGRG 1 cut(s) 118
AoxI GGCC 1 cut(s) 65
ApeKI GCWGC 3 cut(s) 284, 419, 422
AspLEI GCGC 3 cut(s) 22, 64, 74
AsuHPI GGTGA 2 cut(s) 275, 305
AvaI CYCGRG 1 cut(s) 118
BanI GGYRCC 1 cut(s) 19
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 296, 406, 409
BceAI ACGGC 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 12
BfaI CTAG 3 cut(s) 11, 200, 242
BfoI RGCGCY 2 cut(s) 23, 75
BisI GCNGC 4 cut(s) 65, 285, 420, 423
BlsI GCNGC 4 cut(s) 66, 286, 421, 424
BmeT110I CYCGRG 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 21
BmsI GCATC 1 cut(s) 286
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 20
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 1 cut(s) 442
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 1 cut(s) 174
BseNI ACTGG 1 cut(s) 442
BseRI GAGGAG 2 cut(s) 22, 261
BseX3I CGGCCG 1 cut(s) 65
BseXI GCAGC 3 cut(s) 296, 406, 409
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 68
BshFI GGCC 1 cut(s) 67
BshNI GGYRCC 1 cut(s) 19
BsiEI CGRYCG 1 cut(s) 68
BsiHKAI GWGCWC 1 cut(s) 300
BsiHKCI CYCGRG 1 cut(s) 118
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 160
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 1 cut(s) 160
BsmI GAATGC 1 cut(s) 243
BsnI GGCC 1 cut(s) 67
BsoBI CYCGRG 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 300
Bsp143I GATC 1 cut(s) 366
BspACI CCGC 1 cut(s) 64
BspANI GGCC 1 cut(s) 67
BspCNI CTCAG 1 cut(s) 175
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 21
BspPI GGATC 1 cut(s) 361
BspT107I GGYRCC 1 cut(s) 19
BsrI ACTGG 1 cut(s) 442
BssMI GATC 1 cut(s) 366
BssNI GRCGYC 1 cut(s) 20
Bst4CI ACNGT 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 20
BstC8I GCNNGC 3 cut(s) 300, 324, 439
BstDEI CTNAG 2 cut(s) 183, 402
BstFNI CGCG 1 cut(s) 64
BstH2I RGCGCY 2 cut(s) 23, 75
BstHHI GCGC 3 cut(s) 22, 64, 74
BstKTI GATC 1 cut(s) 369
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 366
BstMCI CGRYCG 1 cut(s) 68
BstMWI GCNNNNNNNGC 3 cut(s) 254, 332, 428
BstNSI RCATGY 1 cut(s) 441
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 296, 406, 409
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BstZI CGGCCG 1 cut(s) 65
BsuRI GGCC 1 cut(s) 67
BtsIMutI CAGTG 2 cut(s) 180, 214
Cac8I GCNNGC 3 cut(s) 300, 324, 439
CfoI GCGC 3 cut(s) 22, 64, 74
CseI GACGC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 358
CviAII CATG 2 cut(s) 281, 438
CviJI RGCY 7 cut(s) 56, 67, 287, 326, 335, 401, 419
CviKI_1 RGCY 7 cut(s) 56, 67, 287, 326, 335, 401, 419
CviQI GTAC 1 cut(s) 358
DdeI CTNAG 2 cut(s) 183, 402
DinI GGCGCC 1 cut(s) 21
DpnI GATC 1 cut(s) 368
DpnII GATC 1 cut(s) 366
EaeI YGGCCR 1 cut(s) 65
EagI CGGCCG 1 cut(s) 65
EclXI CGGCCG 1 cut(s) 65
Eco52I CGGCCG 1 cut(s) 65
Eco57I CTGAAG 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 118
EgeI GGCGCC 1 cut(s) 21
EheI GGCGCC 1 cut(s) 21
FaeI CATG 2 cut(s) 284, 441
FaiI YATR 5 cut(s) 282, 311, 395, 434, 439
FaqI GGGAC 1 cut(s) 160
FatI CATG 2 cut(s) 280, 437
Fnu4HI GCNGC 4 cut(s) 65, 285, 420, 423
Fsp4HI GCNGC 4 cut(s) 65, 285, 420, 423
FspBI CTAG 3 cut(s) 11, 200, 242
GlaI GCGC 3 cut(s) 21, 63, 73
GluI GCNGC 4 cut(s) 65, 285, 420, 423
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 2 cut(s) 23, 75
HaeIII GGCC 1 cut(s) 67
HapII CCGG 1 cut(s) 23
HgaI GACGC 1 cut(s) 263
HhaI GCGC 3 cut(s) 22, 64, 74
Hin1I GRCGYC 1 cut(s) 20
Hin1II CATG 2 cut(s) 284, 441
Hin6I GCGC 3 cut(s) 20, 62, 72
HinP1I GCGC 3 cut(s) 20, 62, 72
HincII GTYRAC 2 cut(s) 205, 253
HindII GTYRAC 2 cut(s) 205, 253
HinfI GANTC 1 cut(s) 5
HpaI GTTAAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 23
HphI GGTGA 2 cut(s) 275, 305
Hpy166II GTNNAC 2 cut(s) 205, 253
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 2 cut(s) 79, 330
Hpy8I GTNNAC 2 cut(s) 205, 253
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 82, 254
HpyCH4III ACNGT 1 cut(s) 209
HpyCH4V TGCA 3 cut(s) 171, 277, 284
HpyF10VI GCNNNNNNNGC 3 cut(s) 254, 332, 428
HpyF3I CTNAG 2 cut(s) 183, 402
Hsp92I GRCGYC 1 cut(s) 20
Hsp92II CATG 2 cut(s) 284, 441
HspAI GCGC 3 cut(s) 20, 62, 72
KasI GGCGCC 1 cut(s) 19
KspAI GTTAAC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 366
LmnI GCTCC 1 cut(s) 332
LpnPI CCDG 7 cut(s) 36, 70, 301, 308, 315, 396, 411
Lsp1109I GCAGC 3 cut(s) 296, 406, 409
LweI GCATC 1 cut(s) 286
MaeI CTAG 3 cut(s) 11, 200, 242
MaeIII GTNAC 1 cut(s) 378
MalI GATC 1 cut(s) 368
MboI GATC 1 cut(s) 366
MflI RGATCY 1 cut(s) 366
MhlI GDGCHC 1 cut(s) 300
MluCI AATT 1 cut(s) 194
Mly113I GGCGCC 1 cut(s) 20
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 7 cut(s) 43, 51, 77, 132, 282, 284, 409
MseI TTAA 1 cut(s) 204
MspI CCGG 1 cut(s) 23
Mva1269I GAATGC 1 cut(s) 243
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 3 cut(s) 254, 332, 428
NarI GGCGCC 1 cut(s) 20
NdeII GATC 1 cut(s) 366
NlaIII CATG 2 cut(s) 284, 441
NlaIV GGNNCC 1 cut(s) 21
NmeAIII GCCGAG 1 cut(s) 93
NspI RCATGY 1 cut(s) 441
PaeI GCATGC 1 cut(s) 441
PctI GAATGC 1 cut(s) 243
PkrI GCNGC 4 cut(s) 66, 286, 421, 424
PleI GAGTC 1 cut(s) 13
PluTI GGCGCC 1 cut(s) 23
PpsI GAGTC 1 cut(s) 13
PsiI TTATAA 1 cut(s) 311
PspN4I GGNNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 1 cut(s) 359
RsaNI GTAC 1 cut(s) 358
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 4 cut(s) 65, 285, 420, 423
Sau3AI GATC 1 cut(s) 366
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 300
SetI ASST 4 cut(s) 265, 295, 337, 403
SfaNI GCATC 1 cut(s) 286
SfoI GGCGCC 1 cut(s) 21
SphI GCATGC 1 cut(s) 441
Sse9I AATT 1 cut(s) 194
SsiI CCGC 1 cut(s) 64
SspDI GGCGCC 1 cut(s) 19
SspMI CTAG 3 cut(s) 11, 200, 242
TaaI ACNGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 78, 104, 126
TasI AATT 1 cut(s) 194
TauI GCSGC 1 cut(s) 67
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 2 cut(s) 187, 214
TseI GCWGC 3 cut(s) 284, 419, 422
TspGWI ACGGA 1 cut(s) 375
TspRI CASTG 2 cut(s) 187, 214
XceI RCATGY 1 cut(s) 441
XspI CTAG 3 cut(s) 11, 200, 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.