MD04G1140600.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0024415
Physical Location & Seq
Reverse (-)
22893411 .. 22893881
471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1140600.v1.1.491

Sequence Viewer

Length: 471 bp
ATGTCACTCATTCCTAGTAACATCTTTGTTGGTCACTACGATCCTTTCTGCCACAATGTTTGGGACCCATTTGAGGAATTTCACTATGGAGGCCCAATGATCATCCCACACCATGCTTTCCCAAGTGAAGCAACCTCCTTGGTGACCTCAATGACCGACTGGAAGGAGACCTCAGATGGGCACTTAGTACTCAAGGCACATCTTCCGGGGTTCAAGAGGGAAGAAGTGAAGGTGGATGTGGAAGAAGGTAGGGTTCTTAGTATTAGGGGCGAGAAAAAGGTGGAGAGAGAAGAAAATCACGACGGTTGGCACCGTTTCGAGAGGAGCAGTGGCAAGTTCATTAGGCGTTTGAGGTTGCCTGAGAATGCCAAGGCCGAGAAGATGAAGGTGTTCGTGGAAAATGGAATGCTCACTGTCACGGTTCCTAAGGAGAATGTGGTTAAGTACCATCCTCATACCAGAATGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

157

Amino Acids

18.17

Weight (kDa)

6.87

Isoelectric Point (pI)

56.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 58 - 147 8.9e-22 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0024415)

Species Orthologous Gene IDs
malus_domestica MD04G1140600.v1.1
prunus_persica Prupe.6G266500_v2.0.a1
pyrus_communis pycom04g12820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 309
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 1 cut(s) 77
AfaI GTAC 2 cut(s) 189, 446
AfiI CCNNNNNNNGG 2 cut(s) 73, 177
AgsI TTSAA 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 161
AlwI GGATC 1 cut(s) 35
AoxI GGCC 2 cut(s) 91, 372
ApoI RAATTY 1 cut(s) 77
Asp700I GAANNNNTTC 1 cut(s) 389
AspS9I GGNCC 2 cut(s) 64, 92
AsuC2I CCSGG 1 cut(s) 207
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 64
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 309
BccI CCATC 2 cut(s) 170, 456
BclI TGATCA 1 cut(s) 99
BcnI CCSGG 1 cut(s) 207
BcoDI GTCTC 1 cut(s) 161
BfaI CTAG 2 cut(s) 15, 469
BmcAI AGTACT 1 cut(s) 189
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 64
BmgT120I GGNCC 2 cut(s) 64, 92
BmiI GGNNCC 4 cut(s) 65, 66, 311, 423
BmrFI CCNGG 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 207
BsaI GGTCTC 1 cut(s) 161
BsaJI CCNNGG 3 cut(s) 138, 206, 369
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 177
Bse1I ACTGG 1 cut(s) 164
Bse21I CCTNAGG 1 cut(s) 426
BseDI CCNNGG 3 cut(s) 138, 206, 369
BseGI GGATG 3 cut(s) 102, 241, 448
BseLI CCNNNNNNNGG 2 cut(s) 73, 177
BseMII CTCAG 2 cut(s) 186, 351
BseNI ACTGG 1 cut(s) 164
BseRI GAGGAG 1 cut(s) 337
BseSI GKGCMC 1 cut(s) 183
BshFI GGCC 2 cut(s) 93, 374
BshNI GGYRCC 1 cut(s) 309
BsiSI CCGG 1 cut(s) 206
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 73, 177
BsmAI GTCTC 1 cut(s) 161
BsmFI GGGAC 1 cut(s) 77
BsmI GAATGC 3 cut(s) 370, 411, 468
BsnI GGCC 2 cut(s) 93, 374
Bso31I GGTCTC 1 cut(s) 161
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 40, 99
BspANI GGCC 2 cut(s) 93, 374
BspCNI CTCAG 2 cut(s) 185, 352
BspLI GGNNCC 4 cut(s) 65, 66, 311, 423
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 161
BsrI ACTGG 1 cut(s) 164
BssECI CCNNGG 3 cut(s) 138, 206, 369
BssMI GATC 2 cut(s) 40, 99
BssT1I CCWWGG 2 cut(s) 138, 369
Bst4CI ACNGT 4 cut(s) 305, 314, 415, 421
BstDEI CTNAG 5 cut(s) 172, 184, 257, 360, 426
BstEII GGTNACC 1 cut(s) 142
BstF5I GGATG 3 cut(s) 102, 241, 448
BstKTI GATC 2 cut(s) 43, 102
BstMAI GTCTC 1 cut(s) 161
BstMBI GATC 2 cut(s) 40, 99
BstPI GGTNACC 1 cut(s) 142
BstSCI CCNGG 1 cut(s) 205
BstSLI GKGCMC 1 cut(s) 183
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 2 cut(s) 93, 374
BtsCI GGATG 3 cut(s) 102, 241, 448
BtsI GCAGTG 1 cut(s) 334
BtsIMutI CAGTG 2 cut(s) 334, 411
Cfr13I GGNCC 2 cut(s) 64, 92
Csp6I GTAC 2 cut(s) 188, 445
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 1 cut(s) 113
CviJI RGCY 2 cut(s) 93, 374
CviKI_1 RGCY 2 cut(s) 93, 374
CviQI GTAC 2 cut(s) 188, 445
DdeI CTNAG 5 cut(s) 172, 184, 257, 360, 426
DpnI GATC 2 cut(s) 42, 101
DpnII GATC 2 cut(s) 40, 99
Eco130I CCWWGG 2 cut(s) 138, 369
Eco31I GGTCTC 1 cut(s) 161
Eco47I GGWCC 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 426
Eco91I GGTNACC 1 cut(s) 142
EcoO109I RGGNCCY 1 cut(s) 64
EcoO65I GGTNACC 1 cut(s) 142
EcoT14I CCWWGG 2 cut(s) 138, 369
ErhI CCWWGG 2 cut(s) 138, 369
FaeI CATG 1 cut(s) 116
FaiI YATR 3 cut(s) 87, 114, 456
FaqI GGGAC 1 cut(s) 77
FatI CATG 1 cut(s) 112
FbaI TGATCA 1 cut(s) 99
FokI GGATG 3 cut(s) 89, 248, 435
FspBI CTAG 2 cut(s) 15, 469
HaeIII GGCC 2 cut(s) 93, 374
HapII CCGG 1 cut(s) 206
Hin1II CATG 1 cut(s) 116
HpaII CCGG 1 cut(s) 206
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 1 cut(s) 175
Hpy188III TCNNGA 3 cut(s) 214, 299, 319
Hpy99I CGWCG 1 cut(s) 305
HpyAV CCTTC 4 cut(s) 157, 223, 239, 379
HpyCH4III ACNGT 4 cut(s) 305, 314, 415, 421
HpyCH4V TGCA 1 cut(s) 466
HpyF3I CTNAG 5 cut(s) 172, 184, 257, 360, 426
Hsp92II CATG 1 cut(s) 116
KflI GGGWCCC 1 cut(s) 64
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 2 cut(s) 40, 99
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 3 cut(s) 145, 219, 372
MaeI CTAG 2 cut(s) 15, 469
MaeIII GTNAC 5 cut(s) 3, 17, 32, 142, 415
MalI GATC 2 cut(s) 42, 101
MboI GATC 2 cut(s) 40, 99
MboII GAAGA 5 cut(s) 194, 233, 254, 302, 391
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 1 cut(s) 77
MnlI CCTC 9 cut(s) 67, 83, 145, 157, 181, 210, 315, 345, 462
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 1 cut(s) 441
MspI CCGG 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 207
Mva1269I GAATGC 3 cut(s) 370, 411, 468
NciI CCSGG 1 cut(s) 207
NdeII GATC 2 cut(s) 40, 99
NlaIII CATG 1 cut(s) 116
NlaIV GGNNCC 4 cut(s) 65, 66, 311, 423
NmeAIII GCCGAG 1 cut(s) 400
NmuCI GTSAC 4 cut(s) 3, 32, 142, 415
PctI GAATGC 3 cut(s) 370, 411, 468
PdmI GAANNNNTTC 1 cut(s) 389
PpuMI RGGWCCY 1 cut(s) 64
Psp5II RGGWCCY 1 cut(s) 64
PspEI GGTNACC 1 cut(s) 142
PspN4I GGNNCC 4 cut(s) 65, 66, 311, 423
PspPI GGNCC 2 cut(s) 64, 92
PspPPI RGGWCCY 1 cut(s) 64
RsaI GTAC 2 cut(s) 189, 446
RsaNI GTAC 2 cut(s) 188, 445
SaqAI TTAA 1 cut(s) 441
Sau3AI GATC 2 cut(s) 40, 99
Sau96I GGNCC 2 cut(s) 64, 92
ScaI AGTACT 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 207
SduI GDGCHC 1 cut(s) 183
SetI ASST 8 cut(s) 137, 149, 173, 234, 250, 282, 356, 390
SinI GGWCC 1 cut(s) 64
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 77
SspMI CTAG 2 cut(s) 15, 469
StyD4I CCNGG 1 cut(s) 205
StyI CCWWGG 2 cut(s) 138, 369
TaaI ACNGT 4 cut(s) 305, 314, 415, 421
TaqI TCGA 1 cut(s) 318
TaqII GACCGA 1 cut(s) 170
TasI AATT 1 cut(s) 77
TatI WGTACW 1 cut(s) 187
Tru1I TTAA 1 cut(s) 441
Tru9I TTAA 1 cut(s) 441
TscAI CASTG 2 cut(s) 334, 418
TseFI GTSAC 4 cut(s) 3, 32, 142, 415
Tsp45I GTSAC 4 cut(s) 3, 32, 142, 415
TspDTI ATGAA 2 cut(s) 328, 398
TspRI CASTG 2 cut(s) 334, 418
VpaK11BI GGWCC 1 cut(s) 64
XapI RAATTY 1 cut(s) 77
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 2 cut(s) 15, 469
ZrmI AGTACT 1 cut(s) 189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.