Prupe.6G266500_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0024415
Physical Location & Seq
Reverse (-)
25480861 .. 25481379
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G266500.1

Sequence Viewer

Length: 462 bp
ATGGCATTAATTCCTAGTACCATTTTTGTTGGTCACTATGATCCTTTCTGCCACGATGTTTGGGACCCATTTCAGGAATTCCACTATGGATTTCCAAGAGAAGCAACCTCCTTCACCAACTCCATGATTGATTGGAAGGAGACCTCAGATGGGCACGCATATGTACTCAAGGAAGATCTTCCAGGCTTCAGGAGAGAAGAAATAAAGGTGGATGTGGAGGAGGGTAAGGTTCTTCGCATTAGGGGGGAGAAAAATGTGGAGAGAGAAGAGAAGAGGGACCATTGGCACCGCATCGAAAGGAGCAGCGGCAAGTTCATCAGGCGTTTGAGTTTGCCTGAGAATGCCAAGGCTGACAAGATGAAGGTGTTCATGGAAAATGGAGAGCTCACTGTCACGGTTCCTAAGGAGAAGGTTAATTGCTACCCTCATGCAACCAGAGCCGTTCAAATTTCTGGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

154

Amino Acids

17.93

Weight (kDa)

6.45

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024415)

Species Orthologous Gene IDs
malus_domestica MD04G1140600.v1.1
prunus_persica Prupe.6G266500_v2.0.a1
pyrus_communis pycom04g12820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 285
AciI CCGC 2 cut(s) 289, 306
AclWI GGATC 1 cut(s) 35
AcsI RAATTY 2 cut(s) 77, 447
AcuI CTGAAG 1 cut(s) 172
AfaI GTAC 2 cut(s) 19, 165
AfiI CCNNNNNNNGG 2 cut(s) 73, 150
AgsI TTSAA 1 cut(s) 446
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 1 cut(s) 385
AluI AGCT 1 cut(s) 385
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 35
ApeKI GCWGC 1 cut(s) 303
ApoI RAATTY 2 cut(s) 77, 447
AseI ATTAAT 1 cut(s) 8
Asp700I GAANNNNTTC 2 cut(s) 177, 365
AspS9I GGNCC 2 cut(s) 64, 277
AsuHPI GGTGA 1 cut(s) 106
AvaII GGWCC 2 cut(s) 64, 277
AxyI CCTNAGG 1 cut(s) 402
BaeGI GKGCMC 2 cut(s) 156, 459
BanI GGYRCC 1 cut(s) 285
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 315
BccI CCATC 1 cut(s) 143
BceAI ACGGC 1 cut(s) 425
BciT130I CCWGG 1 cut(s) 183
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 15
BglII AGATCT 1 cut(s) 175
BisI GCNGC 2 cut(s) 304, 307
BlsI GCNGC 2 cut(s) 305, 308
Bme1390I CCNGG 1 cut(s) 183
Bme18I GGWCC 2 cut(s) 64, 277
BmgT120I GGNCC 2 cut(s) 64, 277
BmiI GGNNCC 5 cut(s) 65, 66, 278, 287, 399
BmrFI CCNGG 1 cut(s) 183
BmsI GCATC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 152
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 345
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 150
Bse21I CCTNAGG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 345
BseGI GGATG 1 cut(s) 217
BseLI CCNNNNNNNGG 2 cut(s) 73, 150
BseMII CTCAG 2 cut(s) 159, 327
BseRI GAGGAG 1 cut(s) 233
BseSI GKGCMC 2 cut(s) 156, 459
BseXI GCAGC 1 cut(s) 315
BshNI GGYRCC 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 387
BslFI GGGAC 2 cut(s) 77, 290
BslI CCNNNNNNNGG 2 cut(s) 73, 150
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 2 cut(s) 77, 290
BsmI GAATGC 1 cut(s) 346
Bso31I GGTCTC 1 cut(s) 134
Bsp1286I GDGCHC 3 cut(s) 156, 387, 459
Bsp143I GATC 2 cut(s) 40, 175
BspACI CCGC 2 cut(s) 289, 306
BspCNI CTCAG 2 cut(s) 158, 328
BspLI GGNNCC 5 cut(s) 65, 66, 278, 287, 399
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 285
BspTNI GGTCTC 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 345
BssMI GATC 2 cut(s) 40, 175
BssT1I CCWWGG 1 cut(s) 345
Bst2UI CCWGG 1 cut(s) 183
Bst4CI ACNGT 2 cut(s) 391, 397
Bst6I CTCTTC 2 cut(s) 261, 266
BstC8I GCNNGC 1 cut(s) 156
BstDEI CTNAG 3 cut(s) 145, 336, 402
BstF5I GGATG 1 cut(s) 217
BstKTI GATC 2 cut(s) 43, 178
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 40, 175
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstSLI GKGCMC 2 cut(s) 156, 459
BstV1I GCAGC 1 cut(s) 315
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
Bsu36I CCTNAGG 1 cut(s) 402
BtsCI GGATG 1 cut(s) 217
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 64, 277
Csp6I GTAC 2 cut(s) 18, 164
CspCI CAANNNNNGTGG 2 cut(s) 41, 76
CviAII CATG 3 cut(s) 124, 370, 428
CviJI RGCY 4 cut(s) 186, 350, 385, 440
CviKI_1 RGCY 4 cut(s) 186, 350, 385, 440
CviQI GTAC 2 cut(s) 18, 164
DdeI CTNAG 3 cut(s) 145, 336, 402
DpnI GATC 2 cut(s) 42, 177
DpnII GATC 2 cut(s) 40, 175
Eam1104I CTCTTC 2 cut(s) 261, 266
EarI CTCTTC 2 cut(s) 261, 266
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 134
Eco47I GGWCC 2 cut(s) 64, 277
Eco53kI GAGCTC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 172
Eco81I CCTNAGG 1 cut(s) 402
EcoICRI GAGCTC 1 cut(s) 385
EcoO109I RGGNCCY 1 cut(s) 64
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 345
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 345
FaeI CATG 3 cut(s) 127, 373, 431
FaiI YATR 7 cut(s) 39, 87, 125, 160, 162, 371, 429
FaqI GGGAC 2 cut(s) 77, 290
FatI CATG 3 cut(s) 123, 369, 427
FauNDI CATATG 1 cut(s) 160
Fnu4HI GCNGC 2 cut(s) 304, 307
FokI GGATG 1 cut(s) 224
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 2 cut(s) 304, 307
FspBI CTAG 1 cut(s) 15
GluI GCNGC 2 cut(s) 304, 307
Hin1II CATG 3 cut(s) 127, 373, 431
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 1 cut(s) 148
Hpy188III TCNNGA 2 cut(s) 74, 190
HpyAV CCTTC 4 cut(s) 121, 130, 355, 403
HpyCH4III ACNGT 2 cut(s) 391, 397
HpyCH4V TGCA 1 cut(s) 431
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
HpyF3I CTNAG 3 cut(s) 145, 336, 402
Hsp92II CATG 3 cut(s) 127, 373, 431
KflI GGGWCCC 1 cut(s) 64
Kzo9I GATC 2 cut(s) 40, 175
LmnI GCTCC 1 cut(s) 300
LpnPI CCDG 8 cut(s) 59, 168, 175, 195, 304, 348, 438, 448
Lsp1109I GCAGC 1 cut(s) 315
LweI GCATC 1 cut(s) 300
MaeI CTAG 1 cut(s) 15
MaeIII GTNAC 2 cut(s) 32, 391
MalI GATC 2 cut(s) 42, 177
MboI GATC 2 cut(s) 40, 175
MboII GAAGA 6 cut(s) 170, 185, 209, 224, 278, 283
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 3 cut(s) 156, 387, 459
MluCI AATT 4 cut(s) 9, 77, 415, 447
MnlI CCTC 6 cut(s) 118, 154, 211, 214, 267, 435
MroXI GAANNNNTTC 2 cut(s) 177, 365
MseI TTAA 2 cut(s) 8, 414
MslI CAYNNNNRTG 1 cut(s) 159
MspA1I CMGCKG 1 cut(s) 306
MspR9I CCNGG 1 cut(s) 183
Mva1269I GAATGC 1 cut(s) 346
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeI CATATG 1 cut(s) 160
NdeII GATC 2 cut(s) 40, 175
NlaIII CATG 3 cut(s) 127, 373, 431
NlaIV GGNNCC 5 cut(s) 65, 66, 278, 287, 399
NmuCI GTSAC 2 cut(s) 32, 391
PctI GAATGC 1 cut(s) 346
PdmI GAANNNNTTC 2 cut(s) 177, 365
PkrI GCNGC 2 cut(s) 305, 308
PpuMI RGGWCCY 1 cut(s) 64
PshBI ATTAAT 1 cut(s) 8
Psp124BI GAGCTC 1 cut(s) 387
Psp5II RGGWCCY 1 cut(s) 64
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 5 cut(s) 65, 66, 278, 287, 399
PspPI GGNCC 2 cut(s) 64, 277
PspPPI RGGWCCY 1 cut(s) 64
PsuI RGATCY 1 cut(s) 175
RsaI GTAC 2 cut(s) 19, 165
RsaNI GTAC 2 cut(s) 18, 164
RseI CAYNNNNRTG 1 cut(s) 159
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 8, 414
SatI GCNGC 2 cut(s) 304, 307
Sau3AI GATC 2 cut(s) 40, 175
Sau96I GGNCC 2 cut(s) 64, 277
ScrFI CCNGG 1 cut(s) 183
SduI GDGCHC 3 cut(s) 156, 387, 459
SetI ASST 7 cut(s) 110, 146, 210, 231, 366, 387, 414
SfaNI GCATC 1 cut(s) 300
SinI GGWCC 2 cut(s) 64, 277
SmiMI CAYNNNNRTG 1 cut(s) 159
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 4 cut(s) 9, 77, 415, 447
SsiI CCGC 2 cut(s) 289, 306
SspMI CTAG 1 cut(s) 15
SstI GAGCTC 1 cut(s) 387
StyD4I CCNGG 1 cut(s) 181
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 2 cut(s) 391, 397
TaqI TCGA 1 cut(s) 294
TasI AATT 4 cut(s) 9, 77, 415, 447
TatI WGTACW 1 cut(s) 163
TauI GCSGC 1 cut(s) 309
Tru1I TTAA 2 cut(s) 8, 414
Tru9I TTAA 2 cut(s) 8, 414
TscAI CASTG 1 cut(s) 394
TseFI GTSAC 2 cut(s) 32, 391
TseI GCWGC 1 cut(s) 303
Tsp45I GTSAC 2 cut(s) 32, 391
TspDTI ATGAA 3 cut(s) 304, 358, 374
TspRI CASTG 1 cut(s) 394
VpaK11BI GGWCC 2 cut(s) 64, 277
VspI ATTAAT 1 cut(s) 8
XapI RAATTY 2 cut(s) 77, 447
XmnI GAANNNNTTC 2 cut(s) 177, 365
XspI CTAG 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.