MD04G1168700.v1.1

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0024414
Physical Location & Seq
Reverse (-)
25911582 .. 25912302
721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1168700.v1.1.491

Sequence Viewer

Length: 675 bp
ATGGCACTCCTTTTGTGGATAGTTTGTGTTGCTGTCTCCGGAGCAATCCTTTTTCTCATGATGACAGGAATCTTAAACCATGTCCTACCGACGCAGTCACAGAGAAATGAGTGGCATGAGATTAACAATCAAATTCTCAATGCTCTCTGCACTCTCATGAGTCTTTACCATCACCTGAAAAGGATATACCGTAAGAACGGAACTTACAAGCCCCATGAACAGTTAAACATTATGGTCGTTGTTGTGTTACTCCATCTGGATTGCTTTGCTCGGTATTCCTTGTGTACGCTTAACTTGGGATACAAAAGATCTGAGCGACCGGCTATTGGTGTGTGTACTGCATTGTCAGCTCCCTTGGCACGGAGTATGAAGTTGATAGCAAATCGCAGGATCATATTCTCACAAACCGAACATTTGCATCTAGAAATGAGCAAAAGATTGCTGAATTTGAACCCCAATGGAGAGGGGGGTTTATTTGATTTTCAAGATGATATTGCAACATCACATCTTTCTTTTTTCTGCTCCTTTTGTGTTTTTGGGTGGAACACCGATAGGCTTGGATTTGGCAACATGTACGTGCACATTGCAACATTTCTCCTCTTCCGCACAGCCCCATTTGGATCCTCAGTTTGGCTGCCTCCCATATCGACAACGAAATTGCAAGAGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

225

Amino Acids

25.52

Weight (kDa)

9.17

Isoelectric Point (pI)

50.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 1 - 63 1.4e-17 Protein of unknown function (DUF2985)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024414)

Species Orthologous Gene IDs
malus_domestica MD04G1168700.v1.1 MD12G1182300.v1.1
pyrus_communis pycom12g17140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 38
AciI CCGC 1 cut(s) 604
AclWI GGATC 3 cut(s) 398, 615, 628
AcsI RAATTY 2 cut(s) 132, 445
AfaI GTAC 3 cut(s) 286, 337, 575
AfiI CCNNNNNNNGG 3 cut(s) 326, 360, 630
AflIII ACRYGT 1 cut(s) 570
AgsI TTSAA 2 cut(s) 451, 485
AluBI AGCT 1 cut(s) 350
AluI AGCT 1 cut(s) 350
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 578
AlwI GGATC 3 cut(s) 398, 615, 628
Aor13HI TCCGGA 1 cut(s) 38
ApaLI GTGCAC 1 cut(s) 578
ApeKI GCWGC 1 cut(s) 634
ApoI RAATTY 2 cut(s) 132, 445
AsuHPI GGTGA 1 cut(s) 164
BaeGI GKGCMC 1 cut(s) 582
BamHI GGATCC 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 1 cut(s) 621
BccI CCATC 2 cut(s) 177, 261
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 422
BfuI GTATCC 1 cut(s) 293
BglII AGATCT 1 cut(s) 308
BisI GCNGC 1 cut(s) 635
BlsI GCNGC 1 cut(s) 636
BmiI GGNNCC 1 cut(s) 622
BmsI GCATC 1 cut(s) 427
BsaAI YACGTR 1 cut(s) 577
BsaJI CCNNGG 1 cut(s) 354
BsaWI WCCGGW 1 cut(s) 38
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 360, 630
Bse118I RCCGGY 1 cut(s) 319
Bse3DI GCAATG 1 cut(s) 582
BseAI TCCGGA 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 326, 360, 630
BseMI GCAATG 1 cut(s) 582
BseMII CTCAG 2 cut(s) 303, 639
BseRI GAGGAG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 582
BseXI GCAGC 1 cut(s) 621
BsgI GTGCAG 1 cut(s) 133
Bsh1285I CGRYCG 1 cut(s) 320
BsiEI CGRYCG 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 582
BsiSI CCGG 2 cut(s) 39, 320
BslI CCNNNNNNNGG 3 cut(s) 326, 360, 630
BsmAI GTCTC 1 cut(s) 40
Bsp1286I GDGCHC 1 cut(s) 582
Bsp13I TCCGGA 1 cut(s) 38
Bsp143I GATC 3 cut(s) 308, 390, 620
BspACI CCGC 1 cut(s) 604
BspCNI CTCAG 2 cut(s) 304, 638
BspEI TCCGGA 1 cut(s) 38
BspHI TCATGA 2 cut(s) 57, 156
BspLI GGNNCC 1 cut(s) 622
BspPI GGATC 3 cut(s) 398, 615, 628
BsrDI GCAATG 1 cut(s) 582
BsrFI RCCGGY 1 cut(s) 319
BssAI RCCGGY 1 cut(s) 319
BssECI CCNNGG 1 cut(s) 354
BssMI GATC 3 cut(s) 308, 390, 620
BssT1I CCWWGG 1 cut(s) 354
Bst4CI ACNGT 2 cut(s) 191, 222
Bst6I CTCTTC 1 cut(s) 605
BstBAI YACGTR 1 cut(s) 577
BstDEI CTNAG 2 cut(s) 312, 625
BstKTI GATC 3 cut(s) 311, 393, 623
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 308, 390, 620
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 3 cut(s) 347, 356, 667
BstNSI RCATGY 1 cut(s) 574
BstSLI GKGCMC 1 cut(s) 582
BstV1I GCAGC 1 cut(s) 621
BstX2I RGATCY 2 cut(s) 308, 620
BstYI RGATCY 2 cut(s) 308, 620
BsuI GTATCC 1 cut(s) 293
CciI TCATGA 2 cut(s) 57, 156
Cfr10I RCCGGY 1 cut(s) 319
CseI GACGC 1 cut(s) 100
Csp6I GTAC 3 cut(s) 285, 336, 574
CviAII CATG 6 cut(s) 58, 80, 116, 157, 215, 571
CviJI RGCY 7 cut(s) 211, 323, 350, 556, 611, 634, 670
CviKI_1 RGCY 7 cut(s) 211, 323, 350, 556, 611, 634, 670
CviQI GTAC 3 cut(s) 285, 336, 574
DdeI CTNAG 2 cut(s) 312, 625
DpnI GATC 3 cut(s) 310, 392, 622
DpnII GATC 3 cut(s) 308, 390, 620
Eam1104I CTCTTC 1 cut(s) 605
EarI CTCTTC 1 cut(s) 605
Eco130I CCWWGG 1 cut(s) 354
EcoT14I CCWWGG 1 cut(s) 354
ErhI CCWWGG 1 cut(s) 354
FaeI CATG 6 cut(s) 61, 83, 119, 160, 218, 574
FatI CATG 6 cut(s) 57, 79, 115, 156, 214, 570
Fnu4HI GCNGC 1 cut(s) 635
Fsp4HI GCNGC 1 cut(s) 635
FspBI CTAG 1 cut(s) 422
GluI GCNGC 1 cut(s) 635
HapII CCGG 2 cut(s) 39, 320
HgaI GACGC 1 cut(s) 100
Hin1II CATG 6 cut(s) 61, 83, 119, 160, 218, 574
HinfI GANTC 2 cut(s) 69, 160
HpaII CCGG 2 cut(s) 39, 320
HphI GGTGA 1 cut(s) 164
Hpy166II GTNNAC 3 cut(s) 285, 336, 580
Hpy188I TCNGA 1 cut(s) 313
Hpy188III TCNNGA 6 cut(s) 39, 58, 157, 257, 422, 485
Hpy8I GTNNAC 3 cut(s) 285, 336, 580
Hpy99I CGWCG 1 cut(s) 94
HpyCH4III ACNGT 2 cut(s) 191, 222
HpyCH4IV ACGT 1 cut(s) 576
HpyCH4V TGCA 7 cut(s) 150, 341, 418, 497, 580, 587, 661
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 356, 667
HpyF3I CTNAG 2 cut(s) 312, 625
HpySE526I ACGT 1 cut(s) 576
Hsp92II CATG 6 cut(s) 61, 83, 119, 160, 218, 574
Kpn2I TCCGGA 1 cut(s) 38
Kzo9I GATC 3 cut(s) 308, 390, 620
LmnI GCTCC 3 cut(s) 41, 355, 527
LpnPI CCDG 6 cut(s) 51, 52, 188, 242, 333, 373
Lsp1109I GCAGC 1 cut(s) 621
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 422
MaeII ACGT 1 cut(s) 576
MaeIII GTNAC 2 cut(s) 96, 246
MalI GATC 3 cut(s) 310, 392, 622
MboI GATC 3 cut(s) 308, 390, 620
MboII GAAGA 1 cut(s) 592
MflI RGATCY 2 cut(s) 308, 620
MhlI GDGCHC 1 cut(s) 582
MluCI AATT 3 cut(s) 132, 445, 656
MlyI GAGTC 1 cut(s) 169
MnlI CCTC 4 cut(s) 457, 608, 634, 648
MroI TCCGGA 1 cut(s) 38
MseI TTAA 4 cut(s) 74, 123, 224, 291
MslI CAYNNNNRTG 2 cut(s) 155, 575
MspI CCGG 2 cut(s) 39, 320
MwoI GCNNNNNNNGC 3 cut(s) 347, 356, 667
NdeII GATC 3 cut(s) 308, 390, 620
NlaIII CATG 6 cut(s) 61, 83, 119, 160, 218, 574
NlaIV GGNNCC 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 96
NspI RCATGY 1 cut(s) 574
PagI TCATGA 2 cut(s) 57, 156
PciI ACATGT 1 cut(s) 570
PfeI GAWTC 1 cut(s) 69
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 1 cut(s) 636
PleI GAGTC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 168
Ppu21I YACGTR 1 cut(s) 577
PscI ACATGT 1 cut(s) 570
PspN4I GGNNCC 1 cut(s) 622
PsrI GAACNNNNNNTAC 2 cut(s) 188, 220
PsuI RGATCY 2 cut(s) 308, 620
PsyI GACNNNGTC 1 cut(s) 94
RsaI GTAC 3 cut(s) 286, 337, 575
RsaNI GTAC 3 cut(s) 285, 336, 574
RseI CAYNNNNRTG 2 cut(s) 155, 575
SaqAI TTAA 4 cut(s) 74, 123, 224, 291
SatI GCNGC 1 cut(s) 635
Sau3AI GATC 3 cut(s) 308, 390, 620
SchI GAGTC 1 cut(s) 169
SduI GDGCHC 1 cut(s) 582
SetI ASST 3 cut(s) 177, 352, 579
SfaNI GCATC 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 155, 575
Sse9I AATT 3 cut(s) 132, 445, 656
SsiI CCGC 1 cut(s) 604
SspMI CTAG 1 cut(s) 422
StyI CCWWGG 1 cut(s) 354
TaaI ACNGT 2 cut(s) 191, 222
TaiI ACGT 1 cut(s) 579
TaqI TCGA 1 cut(s) 647
TasI AATT 3 cut(s) 132, 445, 656
TatI WGTACW 1 cut(s) 335
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 4 cut(s) 74, 123, 224, 291
Tru9I TTAA 4 cut(s) 74, 123, 224, 291
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 634
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 2 cut(s) 231, 383
TspGWI ACGGA 2 cut(s) 213, 376
Tth111I GACNNNGTC 1 cut(s) 94
VneI GTGCAC 1 cut(s) 578
XapI RAATTY 2 cut(s) 132, 445
XbaI TCTAGA 1 cut(s) 421
XceI RCATGY 1 cut(s) 574
XspI CTAG 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.