MD12G1182300.v1.1

Protein of unknown function (DUF2985)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0024414
Physical Location & Seq
Reverse (-)
26207403 .. 26209591
2189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1182300.v1.1.491

Sequence Viewer

Length: 1434 bp
ATGGCTACAAGGCTGATGACAAAAATGTTCTTTGTTTTTCGACATCACAAAAGGGACGGCTTACTTAGTGACAAAAATCGCACAAGAGACGAGATCTCAAGTACCGCACCTTCTTCAAATAGCCAAGGTTTTCGAAAACGTTTCAATGGGATTCTTTCTCAGAAGATCGATTGGGAGTCCATGAGGAAAATATGCAAAAAGTGGATCAAGAACCCTCTGAATATGGCACTCCTTTTGTGGATAAGTTGTGTTGCTGTCTCCGGAGCAATCCTTTTTCTTGTGATGACAGGAATGTTAAACCATGTCCTACCGGCGCAGTCTCAGAGAAATGAGTGGTTTGAGATTAACAATCAAATCATCAATGCTCTCTTCACTCTCATGAGTCTTTACCATCACCCGAAAAGGATCCACCACCTTGTACTTCTCTTAAGATGGAAGCCGAAAGATGTCACATTTCTCAGAAAAATATACTGTAAGAACGGAACTTACAAGCCCCATGAACGGATGCACATTCTGGTTGTTGTTGCGTTCCTCCATCTGAATTGCTTTGCTCAGTATGCCTTGTGTACGCTTAACTTGAGATACAAAAGATCTGAGCGATCGGCTATTGGTGTCGGTATCTGCCTTTCAGTTGCAATTGCTGCTCCTGCAATCGCAGGTGTGTACTGCATTGTCAGCCCCCTTGGAAAGGAGTACGAAGTTGATAGCGAATCGCAGGATCATATTCTCACAAATCAAACGAGGAAATTTTCATTTGCATCTAGGAATGAGCAAGGGATTGCTGAATTTGCACCTCAATGGAGAGGGGGTTTATTTGATTTTCGGGAAGATATTGCCATATCTTATCTCTCTTTTTTCTGCACCTTTTGTGTTTTTGGATGGAACGCCAAAAGGCTTGGATTTGGCAACATGTATGTTCACATTGCAACATTTCTCCTCTTCTGCACAGCCCCATTTTGGATCCTCAGTTTAGCTGCCTTCCATATTGACAACAACGTTGCAAGAGAAGCCATGACTATAACCGGCATTGTCCTTTGTATGTTTGGCTTGCTGTACGGTGGCTTCTGGAGAATCCAAATGAGGAAGAGGTTCAACTTGCCGGCGAATAACTTATGCTGTGGAAAGCCTGCGGTAACAGACTGTGCACAATGGCTTTTCTGCTGCTGGTGCTCACTAGCTCAGGAAGTTCGAACTGCGAATTTCTACAGCATAATGGAAGATAACTTGTGCAGGAAGCAAATGTCTGAGCACGAAAATCCCTCACTAGTCCCTTTGCGGCGTGAAAGCGGAAAAACTCAATATAGATCTGGCGCAAATCATCTACCTTCAAGCAGAGTTGAAAAAGATTACCCTAATGTAGACAGACAGCTCCCCATGGAACCCCCTGTTCCATCCAGAATACAGAGAGAAGAAAAAATCGAACCGAAGACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2985 PLAC8

Protein Analysis

478

Amino Acids

55.02

Weight (kDa)

9.45

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2985 PF11204 67 - 145 2.3e-28 Protein of unknown function (DUF2985)
PLAC8 PF04749 267 - 397 9.7e-20 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024414)

Species Orthologous Gene IDs
malus_domestica MD04G1168700.v1.1 MD12G1182300.v1.1
pyrus_communis pycom12g17140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 647
Acc36I ACCTGC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 1359
AccIII TCCGGA 1 cut(s) 260
AciI CCGC 4 cut(s) 105, 1130, 1276, 1287
AclI AACGTT 2 cut(s) 139, 996
AclWI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
AcsI RAATTY 3 cut(s) 746, 785, 1198
AfaI GTAC 6 cut(s) 103, 420, 568, 665, 695, 1055
AfiI CCNNNNNNNGG 3 cut(s) 501, 688, 957
AflII CTTAAG 1 cut(s) 427
AflIII ACRYGT 1 cut(s) 909
AgsI TTSAA 5 cut(s) 117, 145, 1093, 1329, 1340
AhlI ACTAGT 1 cut(s) 1264
AjiI CACGTC 1 cut(s) 1431
AluBI AGCT 3 cut(s) 974, 1178, 1369
AluI AGCT 3 cut(s) 974, 1178, 1369
Alw21I GWGCWC 3 cut(s) 1147, 1172, 1251
Alw26I GTCTC 3 cut(s) 81, 262, 324
Alw44I GTGCAC 1 cut(s) 1143
AlwI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
Aor13HI TCCGGA 1 cut(s) 260
ApaLI GTGCAC 1 cut(s) 1143
ApeKI GCWGC 3 cut(s) 641, 974, 1161
ApoI RAATTY 3 cut(s) 746, 785, 1198
Asp700I GAANNNNTTC 1 cut(s) 1088
AspLEI GCGC 2 cut(s) 316, 1313
AsuHPI GGTGA 1 cut(s) 386
AsuII TTCGAA 2 cut(s) 133, 1189
BaeGI GKGCMC 1 cut(s) 1147
BamHI GGATCC 2 cut(s) 405, 960
BarI GAAGNNNNNNTAC 4 cut(s) 94, 126, 1046, 1078
Bbv12I GWGCWC 3 cut(s) 1147, 1172, 1251
BbvI GCAGC 3 cut(s) 628, 961, 1148
BccI CCATC 5 cut(s) 399, 426, 543, 873, 1399
BceAI ACGGC 1 cut(s) 73
BcoDI GTCTC 3 cut(s) 81, 262, 324
BcuI ACTAGT 1 cut(s) 1264
BfaI CTAG 3 cut(s) 762, 1175, 1265
BfmI CTRYAG 1 cut(s) 1204
BfrI CTTAAG 1 cut(s) 427
BfuAI ACCTGC 1 cut(s) 647
BglII AGATCT 3 cut(s) 93, 590, 1304
BisI GCNGC 4 cut(s) 642, 975, 1162, 1277
BlsI GCNGC 4 cut(s) 643, 976, 1163, 1278
BmgBI CACGTC 1 cut(s) 1431
BmiI GGNNCC 3 cut(s) 407, 962, 1380
BmsI GCATC 2 cut(s) 495, 767
BpmI CTGGAG 1 cut(s) 1087
Bpu10I CCTNAGC 1 cut(s) 1179
Bpu14I TTCGAA 2 cut(s) 133, 1189
BpuEI CTTGAG 2 cut(s) 82, 598
Bsa29I ATCGAT 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 124, 682, 1374
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 2 cut(s) 793, 823
Bsc4I CCNNNNNNNGG 3 cut(s) 501, 688, 957
Bse118I RCCGGY 3 cut(s) 310, 1022, 1099
Bse3DI GCAATG 1 cut(s) 921
BseAI TCCGGA 1 cut(s) 260
BseCI ATCGAT 1 cut(s) 168
BseDI CCNNGG 3 cut(s) 124, 682, 1374
BseGI GGATG 3 cut(s) 510, 884, 1391
BseLI CCNNNNNNNGG 3 cut(s) 501, 688, 957
BseMI GCAATG 1 cut(s) 921
BseMII CTCAG 8 cut(s) 173, 335, 472, 566, 585, 979, 1193, 1236
BseRI GAGGAG 1 cut(s) 926
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 3 cut(s) 628, 961, 1148
BsgI GTGCAG 3 cut(s) 844, 928, 1249
Bsh1285I CGRYCG 1 cut(s) 602
BshVI ATCGAT 1 cut(s) 168
BsiEI CGRYCG 1 cut(s) 602
BsiHKAI GWGCWC 3 cut(s) 1147, 1172, 1251
BsiSI CCGG 4 cut(s) 261, 311, 1023, 1100
BslFI GGGAC 2 cut(s) 68, 1253
BslI CCNNNNNNNGG 3 cut(s) 501, 688, 957
BsmAI GTCTC 3 cut(s) 81, 262, 324
BsmBI CGTCTC 1 cut(s) 81
BsmFI GGGAC 2 cut(s) 68, 1253
Bsp119I TTCGAA 2 cut(s) 133, 1189
Bsp1286I GDGCHC 3 cut(s) 1147, 1172, 1251
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
Bsp19I CCATGG 1 cut(s) 1374
BspACI CCGC 4 cut(s) 105, 1130, 1276, 1287
BspCNI CTCAG 8 cut(s) 172, 334, 471, 565, 586, 978, 1192, 1237
BspDI ATCGAT 1 cut(s) 168
BspEI TCCGGA 1 cut(s) 260
BspHI TCATGA 1 cut(s) 378
BspLI GGNNCC 3 cut(s) 407, 962, 1380
BspMI ACCTGC 1 cut(s) 647
BspPI GGATC 6 cut(s) 212, 400, 413, 726, 955, 968
BspT104I TTCGAA 2 cut(s) 133, 1189
BspTI CTTAAG 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 921
BsrFI RCCGGY 3 cut(s) 310, 1022, 1099
BssAI RCCGGY 3 cut(s) 310, 1022, 1099
BssECI CCNNGG 3 cut(s) 124, 682, 1374
BssMI GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
BssT1I CCWWGG 3 cut(s) 124, 682, 1374
Bst4CI ACNGT 3 cut(s) 473, 1058, 1142
Bst6I CTCTTC 3 cut(s) 374, 944, 1079
BstAFI CTTAAG 1 cut(s) 427
BstAPI GCANNNNNTGC 1 cut(s) 641
BstBI TTCGAA 2 cut(s) 133, 1189
BstC8I GCNNGC 3 cut(s) 1049, 1101, 1128
BstDEI CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
BstDSI CCRYGG 1 cut(s) 1374
BstENI CCTNNNNNAGG 1 cut(s) 686
BstF5I GGATG 3 cut(s) 510, 884, 1391
BstHHI GCGC 2 cut(s) 316, 1313
BstKTI GATC 9 cut(s) 96, 168, 207, 408, 593, 602, 721, 963, 1307
BstMAI GTCTC 3 cut(s) 81, 262, 324
BstMBI GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
BstMCI CGRYCG 1 cut(s) 602
BstMWI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
BstNSI RCATGY 1 cut(s) 913
BstSFI CTRYAG 1 cut(s) 1204
BstSLI GKGCMC 1 cut(s) 1147
BstV1I GCAGC 3 cut(s) 628, 961, 1148
BstX2I RGATCY 5 cut(s) 93, 405, 590, 960, 1304
BstYI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
Bsu15I ATCGAT 1 cut(s) 168
BsuTUI ATCGAT 1 cut(s) 168
BtgI CCRYGG 1 cut(s) 1374
BtrI CACGTC 1 cut(s) 1431
BtsCI GGATG 3 cut(s) 510, 884, 1391
BveI ACCTGC 1 cut(s) 647
Cac8I GCNNGC 3 cut(s) 1049, 1101, 1128
CciI TCATGA 1 cut(s) 378
CfoI GCGC 2 cut(s) 316, 1313
Cfr10I RCCGGY 3 cut(s) 310, 1022, 1099
ClaI ATCGAT 1 cut(s) 168
Csp6I GTAC 6 cut(s) 102, 419, 567, 664, 694, 1054
CviAII CATG 7 cut(s) 181, 302, 379, 497, 910, 1012, 1375
CviQI GTAC 6 cut(s) 102, 419, 567, 664, 694, 1054
DdeI CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
DpnI GATC 9 cut(s) 95, 167, 206, 407, 592, 601, 720, 962, 1306
DpnII GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
Eam1104I CTCTTC 3 cut(s) 374, 944, 1079
EarI CTCTTC 3 cut(s) 374, 944, 1079
Eco130I CCWWGG 3 cut(s) 124, 682, 1374
EcoNI CCTNNNNNAGG 1 cut(s) 686
EcoT14I CCWWGG 3 cut(s) 124, 682, 1374
ErhI CCWWGG 3 cut(s) 124, 682, 1374
Esp3I CGTCTC 1 cut(s) 81
FaeI CATG 7 cut(s) 184, 305, 382, 500, 913, 1015, 1378
FaqI GGGAC 2 cut(s) 68, 1253
FatI CATG 7 cut(s) 180, 301, 378, 496, 909, 1011, 1374
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 4 cut(s) 642, 975, 1162, 1277
FokI GGATG 3 cut(s) 517, 891, 1378
Fsp4HI GCNGC 4 cut(s) 642, 975, 1162, 1277
FspBI CTAG 3 cut(s) 762, 1175, 1265
GlaI GCGC 2 cut(s) 315, 1312
GluI GCNGC 4 cut(s) 642, 975, 1162, 1277
GsuI CTGGAG 1 cut(s) 1087
HapII CCGG 4 cut(s) 261, 311, 1023, 1100
HhaI GCGC 2 cut(s) 316, 1313
Hin1II CATG 7 cut(s) 184, 305, 382, 500, 913, 1015, 1378
Hin6I GCGC 2 cut(s) 314, 1311
HinP1I GCGC 2 cut(s) 314, 1311
HinfI GANTC 5 cut(s) 151, 176, 382, 710, 1071
HpaII CCGG 4 cut(s) 261, 311, 1023, 1100
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 5 cut(s) 567, 664, 919, 1145, 1360
Hpy188I TCNGA 7 cut(s) 162, 219, 324, 461, 540, 595, 1246
Hpy188III TCNNGA 7 cut(s) 208, 261, 379, 824, 1066, 1181, 1395
Hpy8I GTNNAC 5 cut(s) 567, 664, 919, 1145, 1360
HpyAV CCTTC 3 cut(s) 120, 988, 1335
HpyCH4III ACNGT 3 cut(s) 473, 1058, 1142
HpyCH4IV ACGT 3 cut(s) 139, 996, 1430
HpyF10VI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
HpyF3I CTNAG 9 cut(s) 65, 159, 321, 458, 552, 594, 965, 1179, 1245
HpySE526I ACGT 3 cut(s) 139, 996, 1430
Hsp92II CATG 7 cut(s) 184, 305, 382, 500, 913, 1015, 1378
HspAI GCGC 2 cut(s) 314, 1311
Kpn2I TCCGGA 1 cut(s) 260
KroI GCCGGC 1 cut(s) 1099
KroNI GCCGGC 1 cut(s) 1101
Kzo9I GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
LmnI GCTCC 3 cut(s) 263, 649, 1374
Lsp1109I GCAGC 3 cut(s) 628, 961, 1148
LweI GCATC 2 cut(s) 495, 767
MaeI CTAG 3 cut(s) 762, 1175, 1265
MaeII ACGT 3 cut(s) 139, 996, 1430
MaeIII GTNAC 3 cut(s) 68, 448, 1132
MalI GATC 9 cut(s) 95, 167, 206, 407, 592, 601, 720, 962, 1306
MboI GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
MboII GAAGA 8 cut(s) 105, 175, 361, 839, 931, 1096, 1229, 1421
MfeI CAATTG 1 cut(s) 636
MflI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
MhlI GDGCHC 3 cut(s) 1147, 1172, 1251
MluCI AATT 5 cut(s) 541, 636, 746, 785, 1198
MlyI GAGTC 2 cut(s) 185, 391
MroI TCCGGA 1 cut(s) 260
MroNI GCCGGC 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 1088
MseI TTAA 4 cut(s) 296, 345, 428, 573
MslI CAYNNNNRTG 2 cut(s) 377, 796
MspCI CTTAAG 1 cut(s) 427
MspI CCGG 4 cut(s) 261, 311, 1023, 1100
MunI CAATTG 1 cut(s) 636
MwoI GCNNNNNNNGC 7 cut(s) 557, 641, 647, 675, 788, 1007, 1167
NaeI GCCGGC 1 cut(s) 1101
NcoI CCATGG 1 cut(s) 1374
NdeII GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
NgoMIV GCCGGC 1 cut(s) 1099
NlaIII CATG 7 cut(s) 184, 305, 382, 500, 913, 1015, 1378
NlaIV GGNNCC 3 cut(s) 407, 962, 1380
NmuCI GTSAC 2 cut(s) 68, 448
NspI RCATGY 1 cut(s) 913
NspV TTCGAA 2 cut(s) 133, 1189
PagI TCATGA 1 cut(s) 378
PaqCI CACCTGC 1 cut(s) 647
PciI ACATGT 1 cut(s) 909
PdiI GCCGGC 1 cut(s) 1101
PdmI GAANNNNTTC 1 cut(s) 1088
PfeI GAWTC 3 cut(s) 151, 710, 1071
PkrI GCNGC 4 cut(s) 643, 976, 1163, 1278
Ple19I CGATCG 1 cut(s) 602
PleI GAGTC 2 cut(s) 184, 390
PpsI GAGTC 2 cut(s) 184, 390
PscI ACATGT 1 cut(s) 909
Psp1406I AACGTT 2 cut(s) 139, 996
PspN4I GGNNCC 3 cut(s) 407, 962, 1380
PsrI GAACNNNNNNTAC 2 cut(s) 470, 502
PsuI RGATCY 5 cut(s) 93, 405, 590, 960, 1304
PvuI CGATCG 1 cut(s) 602
RsaI GTAC 6 cut(s) 103, 420, 568, 665, 695, 1055
RsaNI GTAC 6 cut(s) 102, 419, 567, 664, 694, 1054
RseI CAYNNNNRTG 2 cut(s) 377, 796
SaqAI TTAA 4 cut(s) 296, 345, 428, 573
SatI GCNGC 4 cut(s) 642, 975, 1162, 1277
Sau3AI GATC 9 cut(s) 93, 165, 204, 405, 590, 599, 718, 960, 1304
SchI GAGTC 2 cut(s) 185, 391
SduI GDGCHC 3 cut(s) 1147, 1172, 1251
SfaNI GCATC 2 cut(s) 495, 767
SfcI CTRYAG 1 cut(s) 1204
SfuI TTCGAA 2 cut(s) 133, 1189
SmiMI CAYNNNNRTG 2 cut(s) 377, 796
SmlI CTYRAG 3 cut(s) 97, 427, 577
SmoI CTYRAG 3 cut(s) 97, 427, 577
SpeI ACTAGT 1 cut(s) 1264
Sse9I AATT 5 cut(s) 541, 636, 746, 785, 1198
SsiI CCGC 4 cut(s) 105, 1130, 1276, 1287
SspMI CTAG 3 cut(s) 762, 1175, 1265
StyI CCWWGG 3 cut(s) 124, 682, 1374
TaaI ACNGT 3 cut(s) 473, 1058, 1142
TaiI ACGT 3 cut(s) 142, 999, 1433
TaqI TCGA 5 cut(s) 40, 133, 168, 1189, 1419
TasI AATT 5 cut(s) 541, 636, 746, 785, 1198
TatI WGTACW 2 cut(s) 418, 663
TauI GCSGC 1 cut(s) 1279
TfiI GAWTC 3 cut(s) 151, 710, 1071
Tru1I TTAA 4 cut(s) 296, 345, 428, 573
Tru9I TTAA 4 cut(s) 296, 345, 428, 573
TseFI GTSAC 2 cut(s) 68, 448
TseI GCWGC 3 cut(s) 641, 974, 1161
Tsp45I GTSAC 2 cut(s) 68, 448
TspDTI ATGAA 2 cut(s) 513, 741
TspGWI ACGGA 2 cut(s) 495, 517
Vha464I CTTAAG 1 cut(s) 427
VneI GTGCAC 1 cut(s) 1143
XagI CCTNNNNNAGG 1 cut(s) 686
XapI RAATTY 3 cut(s) 746, 785, 1198
XceI RCATGY 1 cut(s) 913
XmiI GTMKAC 1 cut(s) 1359
XmnI GAANNNNTTC 1 cut(s) 1088
XspI CTAG 3 cut(s) 762, 1175, 1265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.